Adamts12 (NM_175501) Mouse Tagged ORF Clone

CAT#: MR224137

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Adamts12 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12 (Adamts12)



  "NM_175501" in other vectors (4)

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    • 10 ug


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Specifications

Product Data
Product Name Adamts12 (NM_175501) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Adamts12
Synonyms ADAMTS-12; AI605170
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR224137 representing NM_175501
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATGTGCTCGGGGGAGCTGGCTGGCCAAGCTCTCGATCGTGGCTCAGCTTATTAATTTTGGGGCTT
TTTGCCACGGGAGACAAACTCAGCCCTGGCCGGTTCGCTTCCCAGACCCGAGGCAAGAACATTTTATCAA
GTCCCTGCCAGAATACCACATAGTGAGTCCAGTCCAAGTCGATGCTGGTGGCCATGTTCTGTCCTATGGC
CTGCATCACCCTGTCACTAGCAGCAGGAAGAAGAGAGCTGCAGGTGGTTCTGGGGACCAGCTGTACTACA
GAATTTCGCATGAAGAAAAAGACCTGTTTTTCAATCTGACGGTCAACTGGGAATTTCTTTCCAACGGCTA
TGTCGTGGAGAAGAGATATGGGAACCTTTCCCATGTTAAGATGGTGGCTTCCTCAGGCCAGCCCTGCCAT
CTCAGGGGCACAGTTCTGCAGCAGGGCACCACAGTCGGGATCGGGACAGCAGCTCTCAGTGCCTGCCAAG
GACTGACTGGATTTTTCCATCTACCACATGGAGACTTTTTCATCGAGCCTGTTAAAAAGCATCCACTGAC
CGAGGAAGGGTCCTACCCTCATGTCGTATACAGGAGACAGAGCATCAGAGCTCCAGAGACAAAGGAGCCC
ATCTGTGGATTAAAGGACAGTCTTGATAACTCTGTGAAACAAGAGCTACAGCGGGAGAAGTGGGAGAGGA
AAACCTTGCGGAGCAGAAGCCTCTCCCGACGTTCCATAAGCAAGGAGAGATGGGTGGAGACCCTCGTGGT
GGCCGACACGAAGACGGTAGAGTACCATGGAAGCGAGAACGTGGAGTCGTACATCCTCACCATCATGAAT
ATGGTCACTGGGTTGTTTCATAGCCCAAGCATTGGCAATCTAGTCCACATTGTTGTGGTTCGGCTCATTC
TACTTGAAGAAGAGGAGCAAGGATTGAAAATAGTTCATCATGCAGAGAAGACACTGTCCAGTTTCTGCAA
GTGGCAGAAGAGCATCAATCCCAAGAGTGACCTCAACCCTGTCCATCATGATGTGGCTGTCCTTATCACC
AGAAAGGACATCTGTGCTGGTGTCAATCGCCCTTGTGAGACTCTGGGGCTGTCCCAATTGTCAGGAATGT
GCCAGCCTCACAGGAGCTGTAACATCAATGAAGACTCTGGCCTCCCCCTGGCTTTCACCATTGCACATGA
ACTTGGTCACAGCTTCGGCATCCAGCATGATGGAAAGGAAAATGACTGTGAGCCCGTGGGCAGGCACCCA
TACATCATGTCCCAACAGATTCAGTACGACCCAACTCCACTAACATGGTCGAAATGCAGCAAGGAGTACA
TCACTCGCTTCTTGGACCGAGGCAGGGGGTTCTGTCTTGATGATATCCCCAGCAAGAAAGGCTTGAAGTC
CAACGTCATTGCTCCTGGAGTAATTTACGATGTTCATCACCAGTGCCAGCTCCAATATGGCCCAAATGCT
ACATTCTGCCAGGAAGTGGAAAATGTTTGCCAGACACTGTGGTGCTCGGTGAAAGGCTTCTGTCGCTCTA
AGCTAGATGCTGCTGCAGACGGGACTCGTTGCGGTGAGAAGAAGTGGTGTATGGCTGGAAAGTGTATCAC
AGTGGGGAAGAAACCAGAGAGCATCCCTGGAGGCTGGGGACGCTGGTCACCCTGGTCACACTGTTCTAGA
ACATGTGGGGCAGGAGCTCAGAGCGCAGAGAGGCTCTGCAACAACCCAGAACCAAAATTTGGAGGGAAAT
ACTGCACCGGAGAAAGAAAACGCTATCGCTTATGCAATGTCCACCCCTGCCGCTCAGACACTCCAACTTT
CCGGCAGATGCAGTGCAGTGAATTTGACACAGTCCCCTACAAAAACCAATTCTACCGCTGGTTCCCAGTG
TTTAATGCAGCACATCCTTGTGAGCTGTACTGCAGACCCATAGATGAACAGTTTTCTGAGAGAATGCTGG
AAGCTGTCATTGATGGCACCCCATGCTTCGAAGGTGGCAACAGCAGAAATGTCTGTATTAATGGCATATG
TAAGAGGGTTGGCTGTGACTATGAGATTGATTCCAATGCTACAGAGGATCGCTGTGGTGTGTGCCTTGGA
GATGGCTCTGCCTGCCAGACGGTGAAGAAGCTGTTTAGACAGAAGGAAGGATCTGGTTACGTTGACATTG
GACTTATTCCCAAAGGTGCGAGGGATATAAGGGTGATGGAAATCAAGGCAGCTGGCAATTTCCTGGCCAT
TAGGAGTGAAGATCCGGAAAAATACTACCTCAACGGAGGGTTCATTATCCAGTGGAATGGAAACTATAAA
CTGGCAGGGACCGTTTTCCAGTATGACAGGAAAGGAGATCTGGAGAAACTTATAGCTCCAGGCCCCACCA
ACGAGTCAGTGTGGCTCCAGCTACTTTTCCAGGTGACTAACCCTGGTATCAAGTATGAGTACACAGTCCG
GAAAGATGGCCTTGACAATGACGTGGAGAAGTTGCTGTACTTCTGGCAATTTGGCCGCTGGACAGAATGC
AGTGTAACGTGTGGAACAGGTATCCGCCGGCAGGCTGCTCATTGTGTCAAGAAGGGCCACGGTATAGTGA
AAACGACCTTCTGTAACCCAGAAACACAGCCCAGCGTGAGACAGAAGAAGTGCCATGAAAAGGACTGTCC
ACCCAGGTGGTGGGCAGGGGAGTGGGAAGCGTGTTCAACGACATGTGGGCCCTACGGAGAAAAAAAGAGA
ACGGTGCTGTGCATCCAGACCATGGGCTCGGATGAACAGGCTCTCCCTGCTACAGACTGCCAGCACCTGC
TGAAGCCCAAAGCACTGGTTTCCTGCAACAGAGATATTCTGTGTCCATCAGACTGGACAGTAGGCAACTG
GAGTGAGTGCTCTGTTTCCTGTGGTGGTGGAGTGCGGATTCGCAGTGTCACATGTGCCAAGAACCTTAAT
GAGCCTTGTGACAAGACCCGGAAACCCAACAGTCGAGCTCTGTGCGGCCTCCAGCAGTGCCCATTTAGCC
GTAGAGTTCTGAAACCCAATAAAGATATAGCTCCCAGTGGGAAAAATCAATCGACAGCAGAACATGACCC
CTTCAAGCCCATCCCAGCACCAACATCCAGGCCCACGCCACTGTCCACACCCACAGTGCCTGAGTCTATG
AGCACAAGTACTCCCACAATCAACAGCCTTGGCTCCACCATAGCATCCCAAGAAGACGCAAATGGGATGG
GATGGCAGAATAATTCAACCCAAGCTGAAGAGGGCTCCCACTTCCCTACTTCTTCTGGAAGCACTTCCCA
GGTCCCCGTGACTTCCTGGTCTTTGAGTATCCAACCTGATGATGAGAATGTTTCCAGCTCAGCTATAGGT
CCTACTTCAGAGGGTGACTTTTGGGCAACCACAACAAGTGATTCTGGCTTATCATCTAGCGATGCTATGA
CTTGGCAGGTAACTCCATTTTATAGCACCATGACCACAGATCCAGAAGTGGAGATACATAGTGGTTCAGG
GGAAGACAGTGACCAGCCTTTGAACAAGGACAAAAGCAACTCTGTGATATGGAATAAGATCGGGGTACCT
GAACATGATGCTCCCATGGAGACGGATGCAGAACTACCACTTGGACCTCCACCAACATCTTATATGGGGG
AAGAGCCCTCATGGCCTCCCTTCAGTACAAAAATGGAAGGCTCATTACCTGCCTGGTCCTTCAAAAATGA
GACACCCAGGGATGATGGGATGATCGCTGAAAAATCAAGGAAGATTCCACTCCCTCTTGCAGGAGACCAC
CATCCAGCAACCTCAGAGAAGTTAGAAAACCATGACAAATTGGCATTGCCAAATACTACAAATCCAACCC
AAGGCTTTGGGCCTGTCCTGACTGAAGAAGATGCCTCCAATCTGATTGCCGAGGGGTTTTTGCTAAATGC
TTCTGATTACAAGCATCTTATGAAGGACCACAGCCCTGCATACTGGATTGTTGGAAACTGGAGTAAATGC
TCCACTACCTGTGGGCTGGGGGCCTACTGGAGGAGTGTGGAGTGCAGCTCTGGGGTGGACGCTGACTGCA
CAACCATTCAGAGGCCAGACCCTGCTAAGAAGTGCCACCTCCGCCCCTGTGCTGGCTGGCGAGTGGGAAA
CTGGAGCAAGTGCTCCCGAAATTGCAGTGGAGGCTTCAAGATTCGTGAGGTTCAGTGCATGGACAGCCTG
GACCACCACCGGAGCCTGAGGCCGTTTCACTGTCAATTCCTAGCTGGCGCTCCTCCTCCACTGAGTATGA
GCTGTAACCTGGAGCCTTGTGGAGAATGGCAAGTGGAGCCCTGGAGCCAGTGTTCTAGGTCCTGTGGAGG
AGGTGTTCAGGAGAGAGGAGTGTCTTGTCCTGGAGGCCTCTGTGACTGGACAAAAAGGCCTGCAACCACT
GTGCCCTGCAACAGACACCTTTGCTGTCACTGGGCCACTGGGAACTGGGAGCTGTGTAACACTTCCTGTG
GTGGTGGCTCTCAGAAAAGAACCATTCATTGCATCCCCTCAGAAAACAGTACAACTGAAGATCAAGACCA
GTGCCTCTGTGACCATCAAGTCAAACCCCCAGAATTCCAAACATGCAACCAGCAAGCCTGCAGGAAGAGT
GCTGATTTAACTTGCTTGAAGGACAGACTTTCAATCAGCTTCTGCCAGACACTGAAATCCATGAGGAAGT
GCTCTGTGCCATCAGTGCGAGCCCAGTGCTGTCTCTCGTGTCCACAGGCGCCCAGCATCCACACCCAGAG
GCAAAGAAAACAGCAGTTGCTCCAAAATCATGACATGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR224137 representing NM_175501
Red=Cloning site Green=Tags(s)

MPCARGSWLAKLSIVAQLINFGAFCHGRQTQPWPVRFPDPRQEHFIKSLPEYHIVSPVQVDAGGHVLSYG
LHHPVTSSRKKRAAGGSGDQLYYRISHEEKDLFFNLTVNWEFLSNGYVVEKRYGNLSHVKMVASSGQPCH
LRGTVLQQGTTVGIGTAALSACQGLTGFFHLPHGDFFIEPVKKHPLTEEGSYPHVVYRRQSIRAPETKEP
ICGLKDSLDNSVKQELQREKWERKTLRSRSLSRRSISKERWVETLVVADTKTVEYHGSENVESYILTIMN
MVTGLFHSPSIGNLVHIVVVRLILLEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDLNPVHHDVAVLIT
RKDICAGVNRPCETLGLSQLSGMCQPHRSCNINEDSGLPLAFTIAHELGHSFGIQHDGKENDCEPVGRHP
YIMSQQIQYDPTPLTWSKCSKEYITRFLDRGRGFCLDDIPSKKGLKSNVIAPGVIYDVHHQCQLQYGPNA
TFCQEVENVCQTLWCSVKGFCRSKLDAAADGTRCGEKKWCMAGKCITVGKKPESIPGGWGRWSPWSHCSR
TCGAGAQSAERLCNNPEPKFGGKYCTGERKRYRLCNVHPCRSDTPTFRQMQCSEFDTVPYKNQFYRWFPV
FNAAHPCELYCRPIDEQFSERMLEAVIDGTPCFEGGNSRNVCINGICKRVGCDYEIDSNATEDRCGVCLG
DGSACQTVKKLFRQKEGSGYVDIGLIPKGARDIRVMEIKAAGNFLAIRSEDPEKYYLNGGFIIQWNGNYK
LAGTVFQYDRKGDLEKLIAPGPTNESVWLQLLFQVTNPGIKYEYTVRKDGLDNDVEKLLYFWQFGRWTEC
SVTCGTGIRRQAAHCVKKGHGIVKTTFCNPETQPSVRQKKCHEKDCPPRWWAGEWEACSTTCGPYGEKKR
TVLCIQTMGSDEQALPATDCQHLLKPKALVSCNRDILCPSDWTVGNWSECSVSCGGGVRIRSVTCAKNLN
EPCDKTRKPNSRALCGLQQCPFSRRVLKPNKDIAPSGKNQSTAEHDPFKPIPAPTSRPTPLSTPTVPESM
STSTPTINSLGSTIASQEDANGMGWQNNSTQAEEGSHFPTSSGSTSQVPVTSWSLSIQPDDENVSSSAIG
PTSEGDFWATTTSDSGLSSSDAMTWQVTPFYSTMTTDPEVEIHSGSGEDSDQPLNKDKSNSVIWNKIGVP
EHDAPMETDAELPLGPPPTSYMGEEPSWPPFSTKMEGSLPAWSFKNETPRDDGMIAEKSRKIPLPLAGDH
HPATSEKLENHDKLALPNTTNPTQGFGPVLTEEDASNLIAEGFLLNASDYKHLMKDHSPAYWIVGNWSKC
STTCGLGAYWRSVECSSGVDADCTTIQRPDPAKKCHLRPCAGWRVGNWSKCSRNCSGGFKIREVQCMDSL
DHHRSLRPFHCQFLAGAPPPLSMSCNLEPCGEWQVEPWSQCSRSCGGGVQERGVSCPGGLCDWTKRPATT
VPCNRHLCCHWATGNWELCNTSCGGGSQKRTIHCIPSENSTTEDQDQCLCDHQVKPPEFQTCNQQACRKS
ADLTCLKDRLSISFCQTLKSMRKCSVPSVRAQCCLSCPQAPSIHTQRQRKQQLLQNHDML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_175501
ORF Size 4800 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175501.1, NM_175501.2, NM_175501.3, NP_780710.2
RefSeq Size 8622 bp
RefSeq ORF 4803 bp
Locus ID 239337
Cytogenetics 15 A1
MW 177.8 kDa
Gene Summary This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active protease. Mice lacking the encoded protein exhibit increased angiogenic response and tumor invasion in different models of angiogenesis and, severe inflammation and delayed recovery when subjected to experimental conditions that induce colitis, endotoxic sepsis and pancreatitis. [provided by RefSeq, Jul 2016]

Citations (2)

Other Versions

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