Ythdc2 (NM_001163013) Mouse Untagged Clone

CAT#: MC224492

Ythdc2 (untagged) - Mouse YTH domain containing 2 (Ythdc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ythdc2
• Synonyms: 3010002F02Rik; BC037178
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224492 representing NM_001163013
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGCCGAGCAGCGTCTCGCCGCGTCCGCCGGCTCCTAGCGGAGGCGGCACGGGCGGCGGTGGAG
  GTGGCAGTGGCGGCGGCGGTGGCGGTGGCGGCGGCGGCCCAGCTTCGTGTGGTCCTGGAGGCGGTGGCCG
  GGCCAAGGGCCTCAAAGACATTCGTATCGACGAGGAGGTGAAGATCGCCGTCAACATCGCTCTGGAGCGC
  TTCCGCTACGGTGACCAGAGAGAAATGGAATTCCCTTCTTCTTTGACCAGTACGGAAAGAGCCTTTATTC
  ACCGACTAAGTCAATCTCTTGGTTTAGTTTCTAAAAGTAAAGGAAAGGGAGCAAATAGATACCTAACTGT
  GAAGAAGAAAGATGGATCAGAAACAGCTCATGCAATGATGACCTGTAATTTGACTCATAACACAAAACAT
  GCTGTTAGGAGCCTAATTCAGAGATTTCCTGTCACCAATAAAGAACGTACTGAACTCCTACCTAAAACAG
  AAAGAGGAAATGTGTTTGCAGTTGAAGCTGAAAACCGAGAAATGAGCAAGACAAGCGGGCGACTCAACAA
  TGGCATTCCTCAAGTTCCTGTGAAAAGGGGAGAGTCTGAATTTGACTCATTCAGGCAGTCTTTACCAGTT
  TTTGAGAAACAAGAAGAAATTGTTAAAATAATTAAGGAAAATAAAGTAGTTTTGATAGTAGGAGAAACTG
  GGTCTGGAAAGACCACACAGATTCCTCAGTTCCTGTTAGATGATTGCTTTAAAAATGGCATCCCCTGCCG
  AATATTTTGTACTCAACCAAGACGACTAGCAGCGATTGCTGTGGCTGAAAGAGTTGCTGCAGAGAGAAGA
  GAAAGAATTGGTCAAACAATTGGTTATCAGATCCGATTAGAAAGCAGGGTTTCGCCAAAGACACTTCTGA
  CATTTTGCACTAATGGGGTATTGCTTCGTACATTGATGGCAGGTGATAGCACATTGTCAACTGTGACCCA
  TGTTATTGTGGATGAAGTGCATGAAAGGGATCGATTCAGTGATTTTTTGCTTACTAAGTTAAGAGATTTG
  TTGCAAAAGCACCCAACTTTGAAACTAATTCTTTCGAGTGCTGCCTTGGATGTGAACCTCTTCATAAGAT
  ATTTTGGAAGTTGTCCAGTGATATATATACAGGGAAGACCATTTGAAGTAAAAGAAATGTTTCTGGAAGA
  TATTTTAAGAACTACAGGATATACCAACAAAGAAATGTTAAAGTACAAAAAGGAAAAGCAACGAGAGGAG
  AAACAACAGACCACCCTCACAGAGTGGTACTCAGCTCAAGAGAATACCTTCAAGCCTGAGTCTCAGAGGC
  AGAGAGCTGTTGCTAGTGTGTCTGAAGAGTATGACTTATTGGATGATGGAGGTGATGCTGTCTTCAGTCA
  GCTGACAGAAAAAGATGTGAATTGCCTTGAACCATGGTTAATCAAGGAGATGGATGCCTGCCTTTCTGAC
  ATATGGCTGCATAAAGATGTTGATGCTTTTGCTCAGGTCTTTCATCTTATTTTAACTGAAAATGTTAGTG
  TTGATTATAGGCATAGTGAGACCAGTGCAACAGCTCTGATGGTTGCTGCAGGACGGGGCTTTACGAGCCA
  AGTAGAGCAGTTAATTAGTATGGGAGCCAATGTCCACAGTAAAGCATCAAATGGCTGGATGGCTTTAGAT
  TGGGCTAAGCACTTTGGACAGACTGAAATTGTGGATCTTCTAGAATCTTACAGTGCTTCATTGGAATTTG
  GAAATCTAGATGAAAGTTCCTTGGTTCAGACAAATGGGAATGACCTCAGTGCAGAAGACAGAGAGCTGTT
  GAAAGCTTACCATCATAGTTTTGATGATGAGAAAGTAGACTTGGATTTGATCATGCATCTCCTATATAAT
  ATCTGCCATAGCTGTGATGCTGGTGCAATATTAATTTTTCTACCTGGATATGATGAAATTGTTGGTTTGA
  GGGATCGTATCCTCTTTGATGACAAGCGGTTTGCTGACAATACACATAGGTATCAAGTCTTTATGCTTCA
  TTCAAATATGCAGACATCTGATCAAAAGAAGGTATTAAAAAATCCACCTGCAGGTGTCCGAAAAATAATC
  CTTTCCACCAATATTGCTGAGACGAGCATCACTGTCAATGATGTTGTGTTTGTTATTGATTCTGGTAAGG
  TGAAAGAGAAATCCTTTGATGCACTGAATTTTGTTACAATGTTAAAAATGGTATGGATTTCCAAAGCTAG
  TGCAATACAGCGCAAAGGCAGAGCGGGACGGTGTAGACCTGGAATTTGTTTCCGTCTGTTTAGTAGACTT
  CGATTTCAGAACATGCTGGAATTTCAGACGCCAGAACTCTTGAGAATGCCATTGCAGGAACTTTGTTTAC
  ATACCAAGCTGTTAGCCCCAGTTAACTGCACCATTGCCGACTTCCTTATGAAAGCCCCTGAACCGCCTCC
  AGCTTTAATTGTGAGAAATGCTGTGCAGATGCTTAAGACAATAGATGCAATGGATGCATGGGAAGATCTG
  ACTGAACTTGGGTATCATTTGGCTGATTTGCCAGTAGAACCGCATCTTGGGAAAATGGTCCTGTGTGCAG
  TGGTTTTGAAGTGTCTGGACCCCATCCTTACAATTGCCTGCACGCTCGCTTACCGTGACCCTTTTGTCCT
  GCCTACCCAGGCCTCTCAGAAACGGGCAGCTATGCTTTGTAGGAAACGCTTCACTGCAGGGACTTTCAGT
  GACCACATGGCACTTCTGAGAGCATTTCAGGCATGGCAGAAAGCACGAAGTGATGGGTGGGAGCGAGCCT
  TTTGTGAAAAGAATTTTCTTTCACAAGCTACTATGGAAATCATTATAGGCATGAGAACACAGTTGCTTGG
  TCAGCTTAGAGCATCAGGTTTTGTTAGAGCACGAGGTGGTGGTGACATTCGAGATGTTAATACAAACTCC
  GAGAACTGGGCTGTTGTTAAAGCTGCATTGGTAGCAGGCATGTATCCTAATTTAGTCCACGTGGACAGAG
  AGAATGTAATATTGACAGGGCCAAAGGAAAAGAAAGTACGCTTTCATCCCACTTCAGTTCTCAGTCAACC
  TCAATACAAAAAGATTCCTCCCGCCAATGGTCAGGCTGCAGCGATTCAGGCATTGCCCACAGATTGGCTT
  ATTTATGATGAAATGACCAGAGCCCATCGAATTGCTAATATCAGATGTTGTTCAGCAGTGACACCTGTTA
  CTGTCCTGGTGTTCTGTGGACCAGCAAGACTGGCAAGTAACGCTCTTCAGGAACCTTCATCCTTTAGAGC
  GGATGGTATTCCCAATGACAGCAGTGACAGTGAGATGGAAGACAGAACTACTGCCAATTTGGCTGCTTTG
  AAGCTTGATGAATGGCTTAACTTCAAACTAGAGCCAGAGGCTGCCAGTTTATTGCTACAGCTCAGACAGA
  AGTGGCATAGTTTGTTTTTACGCCGAATGAGAGCGCCATCTAAACCTTGGTCTCAAGTCGATGAAGCTAC
  CATACGAGCAATTATAGCTGTTTTAAGCACTGAAGAACAGTCTGCAGGTTTACAGCAGCCATCTGGAATT
  GGCCAGCGGCCAAGACCCATGTCTTCAGAAGAACTTCCTTTGGCTTCATCTTGGAGATCCAATAATAGTA
  GGAAAAGTACAGCTGACACTGAGTTTGCGGATGGATCTACTACTGGAGAAAGAGTACTGATGAAATCTCC
  ATCTCCAGCCTTACATCCACCTCAGAAGTACAAAGATAGAGGAATTTTACATCCTAAGCGGAGCACCGAT
  GACCGGTCCGATCAATCATCTGTGAAATCCACAGACAGCAGCAGTTACCCAAGTCCTTGTGCCAGCCCCT
  CTCCTCCATCCTCAGGGAAGGGATCAAAATCTCCTTCACCAAGACCAAACATGCCTATTCGTTACTTCAT
  AATGAAGAGTAGCAATCTGAGAAACCTTGAAATTTCTCAACAGAAGGGTATCTGGTCTACAACCCCTAGT
  AATGAGCGGAAGCTAAATCGAGCGTTTTGGGAGAGCAGCATGGTTTACTTGGTATTTTCTGTTCAAGGAT
  CTGGACATTTCCAGGGATTTTCTAGGATGTCTTCTGAGATAGGAAGGGAGAAGAGCCAGGACTGGGGGTC
  AGCTGGACTAGGTGGAGTATTTAAAGTGGAGTGGATACGCAAAGAAAGCCTTCCCTTTCAGTTTGCACAC
  CATTTACTCAATCCTTGGAACGACAACAAGAAAGTACAGATAAGCAGAGATGGGCAGGAGCTAGAACCTC
  AGGTTGGGGAACAGTTGCTCCAGTTATGGGAACGCCTTCCATTGGGAGAAAAAACAACTTCTGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163013
• Insert Size: 4338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163013.1, NP_001156485.1
• RefSeq Size: 6299 bp
• RefSeq ORF: 4338 bp
• Locus ID: 240255
• UniProt ID: B2RR83
• Cytogenetics: 18 B3
• Gene Summary: 3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells (PubMed:28380054, PubMed:28809393, PubMed:29087293, PubMed:29033321, PubMed:29360036). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability (PubMed:29360036). Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs (PubMed:29033321). Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease (PubMed:29033321). Required for both spermatogenesis and oogenesis (PubMed:28809393, PubMed:29033321).[UniProtKB/Swiss-Prot Function]