Fancd2 (NM_001033244) Mouse Untagged Clone

CAT#: MC224490

Fancd2 (untagged) - Mouse Fanconi anemia, complementation group D2 (Fancd2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fancd2
• Synonyms: 2410150O07Rik; AU015151; BB137857; FA-D2; FA4; FACD; FAD; FANCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224490 representing NM_001033244
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCCAAAAGACGTCGGCTAGATTCTGAGGATAAAGAAAACCTGACAGAAGATGCCTCCAAAACCA
  TGCCCCTTTCCAAGCTGGCAAAGAAGTCTCACAATTCTCATGAAGTTGAAGAAAATGGCAGTGTCTTTGT
  AAAGCTTCTTAAGGCTTCAGGACTCACTCTTAAAACTGGAGAGAACCAAAATCAGCTAGGTGTGGATCAG
  GTAATCTTCCAAAGGAAGCTCTTTCAGGCCTTGAGGAAGCATCCTGCTTATCCCAAAGTAATAGAAGAGT
  TTGTTAATGGCCTGGAGTCCTACACTGAGGACAGTGAGAGTCTCAGGAACTGCCTGCTGTCTTGTGAGCG
  CCTGCAGGATGAGGAAGCCAGCATGGGCACATTTTACTCCAAGAGTCTGATCAAGCTACTTCTGGGGATT
  GACATTTTACAGCCTGCCATTATCAAAATGTTATTTGAAAAAGTGCCTCAGTTTCTTTTTGAAAGTGAGA
  ACAGAGATGGAATCAACATGGCCAGACTCATTATCAATCAACTAAAATGGCTGGATAGAATTGTGGATGG
  CAAGGACCTCACGGCCCAGATGATGCAGTTGATCAGTGTTGCTCCCGTGAACTTACAGCATGACTTCATC
  ACGAGCCTTCCTGAAATCCTAGGGGATTCCCAGCATGCTAATGTGGGGAAAGAGCTTGGCGAGCTGCTGG
  TGCAGAATACTTCCCTGACTGTTCCAATTTTGGATGTCTTTTCCAGTCTCCGACTTGACCCCAACTTCCT
  GTCCAAGATCCGCCAGTTGGTGATGGGCAAGCTGTCATCTGTCCGTCTAGAGGATTTCCCTGTGATTGTA
  AAGTTCCTTCTTCATTCTGTAACAGACACCACTTCCCTTGAGGTCATTGCCGAGCTTCGGGAGAACTTGA
  ACGTCCAGCAGTTTATTTTGCCGTCACGAATTCAGGCTTCCCAAAGCAAATTGAAAAGTAAAGGACTAGC
  AAGCTCTTCAGGAAATCAAGAGAACAGTGATAAAGACTGTATTGTTCTTGTCTTTGATGTAATAAAGTCA
  GCCATTAGATATGAGAAAACCATTTCAGAGGCCTGGTTTAAGGCAATTGAACGCATTGAGTCCGCGGCTG
  AACATAAGGCTTTGGACGTGGTCATGCTGCTCATCATCTACAGCACCAGCACGCAGACCAAGAAGGGCGT
  GGAGAAGCTGCTGAGAAACAAGATTCAGTCAGACTGCATTCAAGAACAGCTGCTTGACAGTGCGTTCTCT
  ACACATTACCTGGTTCTTAAGGATATTTGCCCATCTATTCTTTTGCTGGCTCAGACTTTGTTTCACTCTC
  AAGACCAGAGGATCATTTTGTTTGGCAGTCTTCTGTACAAATATGCTTTTAAGTTTTTTGATACTTACTG
  CCAGCAGGAAGTGGTTGGTGCCCTAGTCACCCATGTCTGCAGTGGGACTGAGGCTGAAGTCGACACTGCA
  CTGGATGTCCTCCTGGAGCTGATTGTGCTAAACGCCTCTGCTATGAGGCTCAATGCTGCTTTTGTTAAGG
  GCATCTTAGATTATTTGGAAAATATGTCCCCTCAGCAAATACGAAAAATCTTCTGTATTCTCAGCACTCT
  TGCATTTAGCCAACAGCCCGGAACCAGCAACCATATCCAGGACGACATGCACCTGGTGATCCGGAAGCAG
  CTCTCTAGCACTGTGTTCAAGTACAAGCTCATTGGGATCATTGGTGCAGTCACCATGGCCGGCATCATGG
  CGGAAGACAGAAGTGTACCATCTAACTCATCCCAGAGGAGCGCCAATGTGAGCAGTGAGCAGCGCACACA
  GGTGACTTCTTTGCTACAACTAGTTCATTCTTGCACTGAGCACTCTCCTTGGGCCTCTTCTCTGTATTAT
  GATGAATTTGCCAACCTGATCCAAGAAAGGAAGTTGGCTCCAAAAACCTTGGAGTGGGTTGGGCAGACCA
  TCTTCAATGATTTCCAAGATGCCTTTGTGGTAGACTTCTGTGCTGCTCCAGAGGGTGACTTTCCATTTCC
  TGTGAAAGCGCTCTATGGACTGGAAGAGTACAGCACTCAAGACGGCATTGTCATCAACCTCCTGCCGCTG
  TTCTATCAGGAATGTGCAAAAGATGCCAGTCGAGCGACATCACAAGAATCGAGCCAGAGATCAATGTCTT
  CTTTGTGCCTGGCTTCCCATTTCCGGCTGCTGAGACTTTGCGTGGCAAGACAACATGATGGAAACTTGGA
  TGAGATCGATGGTCTCTTAGATTGTCCCCTGTTCCTCCCTGACCTGGAACCTGGAGAGAAACTGGAGTCC
  ATGTCTGCTAAAGACCGTTCGCTTATGTGTTCGCTCACATTCCTAACTTTCAACTGGTTCCGAGAGGTTG
  TGAATGCCTTCTGCCAACAAACATCTCCTGAGATGAAGGGCAAGGTTCTTAGTCGGCTAAAGGACCTTGT
  AGAACTTCAGGGAATCCTAGAGAAGTACTTGGCAGTCATCCCAGACTATGTTCCGCCTTTCGCAAGCGTT
  GACTTGGACACTTTAGATATGATGCCTAGGAGCAGTTCTGCTGTTGCAGCAAAAAACAGAAACAAGGGAA
  AGACGGGGGGAAAGAAACAAAAAGCTGATAGCAACAAAGCATCCTGTTCGGACACACTTCTAACAGAAGA
  CACTTCAGAGTGTGACATGGCGCCATCTGGGAGAAGCCACGTAGACAAGGAGTCCACAGGGAAGGAAGGA
  AAGACGTTTGTGTCACTGCAGAATTACCGCGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTC
  TACATTCTGGACTTGTGACCAAGTTCATCTTAGACACTGAAATGCACACTGAAGCTACAGAGGTCGTACA
  GCTGGGGCCTGCTGAGCTGCTCTTCTTGCTGGAAGATCTTTCCCAGAAGCTAGAGAATATGCTGACTGCT
  CCTTTTGCCAAGAGAATCTGCTGCTTTAAGAATAAAGGAAGGCAGAATATTGGCTTCTCACATCTTCATC
  AGAGATCTGTCCAGGACATTGTGCACTGTGTGGTTCAGCTGCTAACCCCGATGTGTAACCATCTGGAGAA
  CATTCACAACTTCTTTCAGTGCTTAGGTGCTGAGCATCTCAGTGCAGATGACAAGGCGAGAGCGACAGCT
  CAGGAGCAGCACACCATGGCCTGCTGCTACCAGAAGCTGCTGCAGGTCTTGCACGCGCTCTTTGCGTGGA
  AGGGATTTACTCACCAATCAAAGCACCGCCTCCTGCACTCAGCCCTTGAGGTCCTCTCGAACCGACTAAA
  GCAGATGGAACAGGACCAGCCCTTGGAGGAACTGGTCAGCCAGAGCTTCAGTTACTTGCAGAACTTCCAC
  CATAGTGTTCCCAGTTTCCAGTGTGGTCTCTACCTTCTCAGACTTCTGATGGCCCTTCTGGAGAAGTCTG
  CAGTACCTAACCAGAAGAAAGAAAAACTTGCCTCTCTGGCCAAACAGCTGCTTTGCCGAGCATGGCCTCA
  TGGGGAAAAAGAGAAGAACCCCACTTTTAATGACCACCTGCATGATGTGCTTTACATCTACTTGGAGCAC
  ACAGACAATGTTCTGAAGGCCATAGAGGAGATCACTGGTGTTGGTGTCCCAGAACTGGTCAGTGCTCCGA
  AAGACGCCGCCTCCTCTACATTCCCTACGTTGACCAGGCACACCTTTGTCATATTCTTCCGTGTGATGAT
  GGCTGAACTCGAGAAGACGGTGAAGGGTCTTCAGGCTGGCACAGCAGCAGATTCGCAGCAGGTTCACGAA
  GAGAAGCTCCTCTATTGGAACATGGCTGTCCGAGATTTCAGCATCCTTCTCAATCTGATGAAAGTATTTG
  ACAGTTATCCTGTTCTGCATGTGTGTTTAAAGTATGGCCGTCGCTTTGTGGAGGCATTTCTGAAGCAATG
  TATGCCACTCCTCGACTTCAGCTTTAGAAAGCATCGGGAAGATGTTCTGAGCTTGCTGCAAACCCTTCAG
  TTGAACACGAGGCTACTTCATCACCTTTGTGGACACTCCAAGATTCGCCAGGACACAAGACTCACCAAGC
  ATGTGCCTTTACTCAAAAAGTCACTGGAACTGTTAGTTTGCAGAGTCAAAGCCATGCTTGTCCTCAACAA
  CTGTAGAGAGGCTTTCTGGTTGGGTACTCTCAAAAACCGAGACTTACAGGGTGAAGAAATTATTTCCCAG
  GATCCCTCTTCCTCAGAGAGCAATGCAGAGGACAGTGAGGATGGCGTGACATCTCACGTCTCCAGGAACA
  GAGCAACAGAGGATGGGGAAGATGAAGCAAGTGATGAACAGAAGGACCAGGACAGTGATGAAAGTGACGA
  CAGCTCCAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033244
• Insert Size: 4353 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033244.3, NP_001028416.2
• RefSeq Size: 4780 bp
• RefSeq ORF: 4353 bp
• Locus ID: 211651
• UniProt ID: Q80V62
• Cytogenetics: 6 E3
• Gene Summary: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress (By similarity). Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).