Syne1 (NM_153399) Mouse Untagged Clone

CAT#: MC224453

Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syne1
• Synonyms: 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224453 representing NM_153399
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCTCCAGAGCATCTTCCCGGTCCCATCGGGACATCACCAATGTGATGCAGAGGCTACAAGATG
  AACAAGAGATTGTACAGAAACGTACCTTCACAAAATGGATCAATTCCCATCTGGCCAAGCGGAAGCCTCC
  CATGGTGGTGGATGACCTTTTTGAAGACATGAAAGATGGCATCAAGCTGCTTGCTTTACTGGAGGTCCTG
  TCTGGGCAGAAACTGCCTTGTGAACAAGGACACCGGGTGAAGCGTATTCATGCTGTGGCTAACATTGGCA
  CCGCACTCAAATTCCTTGAAGGAAGGAAGTCCATGTACAGAGGATCACCGATTAAATTAGTCAACATTAA
  TGCTACTGATATTGCTGATGGCCGACCATCAATCGTTCTTGGGCTGATGTGGACCATTATCCTGTATTTC
  CAGATAGAAGAGTTGACCAGCAACCTACCACAGCTGCAGTCTCTATCCAGCAGTGCTTCCTCTGTGGACA
  GCATGGTCAGCACCGAGACTGCCAGCCCACCCAGCAAACGAAAGGTGGCTGCCAAGATCCAGGGAAATGC
  CAAGAAAACTCTATTAAAGTGGGTCCAGCACACAGCAGGCAAGCAGATGGGAATAGAAGTGAAAGACTTC
  GGGAAGAGTTGGAGAACTGGGTTGGCCTTTCATTCAGTCATCCACGCTATCCAGCCTGAGCTGGTGGACC
  TGGAGAAGGTGAAGACTAGATCTAACAGGGAAAACCTGGAGGACGCCTTCACGATCGCTGAAACACAGCT
  AGGCATCCCCAGACTGCTTGATCCCGAAGATGTGGATGTGGACAAGCCAGATGAAAAGTCTATCATGACT
  TACGTGGCCCAGTTTCTAACACAATACCCTGACATTCATGGTGCAGGCTGTGATGGGCAAGAGGATGATA
  GGGATGACAGACTAATTTTGAAGGAAACAAAAGTTTGGATAGAACAATTTGAGAGAGATTTTACAAGGGC
  ACAGATGACCGAATCAAGTCTGCAGGACAAATATCAGGCATTTAAGCACTTCAGAGTTCAATATGAAATG
  AAAAGGAAACAAGTGGAGCATATAATACAGCCATTGCAAAGAGATGGCAAACTGACGCTCGACCAAGCCT
  TGGTCAAGCAGTGCTGGGAGAGAGTATCATCCCGGCTCTTCGATTGGCATATACAGCTGGATAAATCTCT
  CCCTGCACCTCTGGGCACTATAGGTGCTTGGCTATACAGGGCAGAAGTGGCCCTGAGAGAAGAGATCACC
  ATTCAGCAGGTTCACGAGGAGACAGCAAACACAATCCAACGGAAACTTGAGCAACATAAGGATCTGCTTC
  AAAATACAGATGCCCACAAAAGGGCGTTCCATGAAATCTACCAGACCAGGTCTGTCAATGGGATCCCGAT
  GCCTCCTGATCAGCTAGAGGACATGGCTGAGAGGTTTCATTTTGTGTCCTCCACATCCGAGCTGCACTTA
  ATGAAAATGGAGTTTCTCGAACTGAAGTACCGTCTGCTGTCCCTGCTGGTCCTCGCTGAGTCAAAGCTGA
  AGTCCTGGATCATCAAGTATGGGAGGCGAGAGTCTGTGGAGCTGCTGCTGCAAAGCTATATCTCTTTTAT
  AGAAAACAGCAAGTTCTTTGAACAGTATGAAGTGACATACCAGATCCTGAAACAGACAGCTGACATATAC
  GTCAAAGCAGAGGGCTCAGTGGAGGAAGCTGAGAACGTGATGAAATTCATGAGTGAAGCCACTGCCCAGT
  GGAGGAACCTCTCCGTGGAAGTGCGGAGTGTACGGAGCATGCTGGAGGAGGTGATCTCCAACTGGGATCG
  CTATGGTGATACCGTGGCTAGTCTCCAAGCCTGGCTAGAAGATGCAGAGAAAATGCTAAGCCAATCAGAA
  CATGCCAAGAAGGATTTTTTTCGAAATCTGCCTCACTGGATTCAGCAGCACACAGCCATGAACGATGCCG
  GGAATTTCCTGATTGAAACCTGTGATGAGATTGTTTCTCGAGACCTTAAACAGCAGTTGCTCTTATTGAA
  CGGTCGATGGAGGGAGCTCTTCATGGAAGTCAAGCAATATGCAAGAGCAGACGAGATGGACAGAATGAAG
  AAAGAGTATATAGACGTCACTACCACCCTCTTTGGTTTTGCAACTGAAGCCCACAGGAAACTCTCTGAGC
  CTTTAGAAGTCTCTTTTATCAATGTCAAGTTATTAATTCAAGACTTGGAGGATCTGGAGAAGAGGGTGCC
  TGTGATGGATGCTCAGTACAAGATGATTGCCAAGAAGGCACATCTCTTTGCCAAAGAAAGTCCCCAAGAA
  GAAGCGAATGAAATGCTTACAACCATGTCCAAGCTCAAAGAACAGCTCTCCAAGGTCAAAGAATGTTGCT
  CCCCACTGTTGTACGAGGCCCAGCAACTGACGGTTCCTTTGGAAGAACTGGAGACGCAAATCACGTCCTT
  CTATGACTCCCTTGGAAAAATCAATGAGATTCTATCAGTTCTTGAGCAAGAAGCACAATCTAGCACTCTT
  TTTAAGCAAAAACACCAGGAACTGTTAGCTTCTCAAGAGAACTGTAAGAAATCCTTGACACTCATAGAGA
  AGGGCAGCCAAAGTGTTCAGAAGCTGGTGACCTCGAGCCAGGCACGAAAGCCCTGGGATCACACAAAACT
  GCAGAAGCAGATAGCAGATGTCCATCATGCATTCCAGAGCATGATCAAGAAAACTGGAGACTGGAAGAAG
  CACGTGGAAGCCAACAGCCGTTTGATGAAGAAATTTGAGGAGTCTCGGGCCGAGTTGGAGAAGGTGCTTA
  GGGTTGCTCAGGAGGGCCTAGAGGAGAAGGGGGACCCCGAGGAACTCCTTCGGAGACACACGGAGTTCTT
  CAGCCAACTGGACCAGAGGGTCCTCAATGCTTTCCTAAAAGCCTGTGATGAGCTAACTGACATCCTCCCA
  GAGCAGGAGCAACAAGGCCTACAGGAGGCTGTTCGAAAGCTACACAAACAGTGGAAGGATCTTCAGGGAG
  AAGCGCCTTATCATTTGCTCCATCTGAAAATCGCTGTGGAGAAAGACAGATTCTCAGCTGCTGTGGAAGA
  GTGCAGAGCTGAGCTGGAGCAAGAGACCAAGCTGGCTCCTCAAGAAGGCAGCGAGAAGATCATAAAGGAA
  CACAGGGTTTTCTTCAGTGACAAGGGTCCTCACCATCTCTGTGAGAAGAGACTACAGCTCATAGAGGAAT
  TGTGTGGGAAACTCCCCGTCCAGGACCCAGTAAGGGACACATGTGGAGCCTGTCACACAGCTCTCAAAGA
  GCTCAAAGCTTCCATTGACAACACTTACACAATGCTCGTGGATGACCCAGACAAGTGGAAGGACTATACC
  AGCAGGTTTTCTGAGTTCTCCTCTTGGGTGTCTGCAAAGAAGGCATGTTTGAAGAAAATCAAGGATGAGC
  CCATTGACACTGGCAACCACGACGAGGTTAAGCATATGGTGGATGAGATCAGAAATGACATTACCAAGAA
  AGGAGAGAGTCTCAGCTGGCTGAAATCCAGGCTTAAGTATTTGATTGATATTTCTTCAGAGAATGAAGCC
  CAAAAGCGAGGAGATGAGCTGGCAGAATTATCAAGCTCTTTCAAGGCTCTTGTGGCATTACTGTCAGAGG
  TCGAAAAGCTTCTAAGCAACTTTGGAGAATGCGTTCAGTACAAGGAAATCGTGAAAAGTTCCCTTGAGGG
  GTTGATATCGGGCCCCCAAGAAAGCAAGGAAGAGGCTGAGATGATCCTGGACTCGAAAAATCTGCTTGAG
  GCACAGCAGTTGCTCCTGCATCACCAGCAAAAGACAAAGATGATCTCAGCAAAGAAGAGAGACCTCCAGG
  AGCAGATGGAGCAGGCACAGCAGGGTGGGCAGGCAGGCCCAGGTCAAGAGGAGCTGCGGAAGCTGGAGAG
  CACCCTCACAGGCCTGGAGCAGAGCAGGGAGAGGCAGGAACGTCGCATCCAGGTCAGCTTGAGGAAATGG
  GAGCGGTTTGAGACAAACAAGGAGACGGTGGTCAGATATCTTTTCCAGACAGGTTCCAGCCACGAACGCT
  TCTTGAGCTTCAGCAGTTTGGAAAGCCTGTCATCAGAGTTGGAGCAGACAAAGGAGTTTTCTAAGAGGAC
  GGAAAGCATTGCCACCCAGGCTGAGAACCTGGTCAAGGAAGCCGCAGAGTTGCCGCTGGGGCCCAGGAAT
  AAGCGTGTGCTTCAGCGGCAGGCCAAGTCAATCAAGGAGCAGGTCACAACCCTGGAAGACACGCTTGAAG
  AAGAGTATGTGCTTCACCACTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153399
• Insert Size: 4296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153399.2, NP_700448.2
• RefSeq Size: 6982 bp
• RefSeq ORF: 4296 bp
• Locus ID: 64009
• Cytogenetics: 10 A1
• Gene Summary: Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in both UTRs and has multiple differences in the coding region compared to variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (1) is shorter and has distinct N- and C-termini compared to isoform 4.