Wrn (NM_011721) Mouse Untagged Clone

CAT#: MC224398

Wrn (untagged) - Mouse Werner syndrome homolog (human) (Wrn), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wrn
• Synonyms: AI846146
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224398 representing NM_011721
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCACTTCACTACAGCGGAAATTTCCAGAATGGATGTCTATGCAGAGTCAAAGATGTGCTACAG
  AAGAAAAGGCCTGCGTTCAGAAGAGTGTTCTTGAAGATAACCTCCCATTCTTAGAATTCCCTGGATCCAT
  TGTTTACAGTTATGAAGCTAGTGATTGCTCCTTCCTGTCTGAAGACATTAGCATGCGTCTGTCTGATGGC
  GATGTGGTGGGATTTGACATGGAATGGCCGCCCATATACAAGCCAGGGAAAAGAAGCAGAGTCGCAGTGA
  TCCAGTTGTGTGTGTCTGAGAGCAAATGTTACTTGTTTCACATTTCTTCCATGTCAGTTTTCCCCCAGGG
  ATTAAAAATGTTACTAGAAAACAAATCAATTAAGAAGGCAGGGGTTGGGATTGAAGGGGACCAGTGGAAA
  CTTCTGCGTGATTTTGACGTCAAGTTGGAGAGTTTTGTGGAGCTGACGGATGTTGCCAATGAAAAGTTGA
  AGTGCGCAGAGACCTGGAGCCTCAATGGTCTGGTTAAACACGTCTTAGGGAAACAACTTTTGAAAGACAA
  GTCCATCCGCTGCAGCAATTGGAGTAATTTCCCCCTCACTGAGGACCAGAAACTGTATGCAGCCACTGAT
  GCTTATGCTGGTCTTATCATCTATCAAAAATTAGGAAATTTGGGTGATACTGCGCAAGTGTTTGCTCTAA
  ATAAAGCAGAGGAAAACCTACCTCTGGAGATGAAGAAACAGTTGAATTCAATCTCCGAAGAAATGAGGGA
  CCTAGCCAATCGTTTTCCTGTCACTTGCAGAAATTTGGAAACTCTCCAGAGGGTTCCTGTAATATTGAAG
  AGTATTTCAGAAAATCTCTGTTCATTGAGAAAAGTGATCTGTGGTCCTACAAACACTGAGACTAGACTGA
  AGCCGGGCAGTAGTTTTAATTTACTGTCATCAGAGGATTCAGCTGCTGCTGGAGAAAAAGAGAAACAGAT
  TGGAAAACATAGTACTTTTGCTAAAATTAAAGAAGAACCATGGGACCCAGAACTTGACAGTTTAGTGAAG
  CAAGAGGAGGTTGATGTATTTAGAAATCAAGTGAAGCAAGAAAAAGGTGAATCTGAAAATGAAATAGAAG
  ACAATCTGTTGAGAGAAGATATGGAAAGAACTTGTGTGATTCCTAGTATTTCAGAAAATGAACTCCAAGA
  TTTGGAACAGCAAGCTAAAGAAGAAAAATATAATGATGTTTCTCACCAACTTTCTGAGCATTTATCTCCC
  AATGATGATGAGAATGACTCCTCCTATATAATTGAAAGTGATGAAGATTTGGAAATGGAGATGCTGAAGT
  CTTTAGAAAACCTAAATAGTGACGTGGTGGAACCCACTCACTCTACATGGTTGGAAATGGGAACCAATGG
  GCGTCTTCCTCCTGAGGAGGAAGATGGACACGGAAATGAAGCCATCAAAGAGGAGCAGGAAGAAGAGGAC
  CATTTATTGCCGGAACCCAACGCAAAGCAAATTAATTGCCTCAAGACCTATTTCGGACACAGCAGTTTTA
  AACCGGTTCAGTGGAAAGTCATCCATTCTGTATTAGAAGAGAGAAGAGATAATGTTGTTGTCATGGCAAC
  TGGATATGGGAAGAGTCTGTGCTTCCAGTATCCGCCTGTTTATACAGGCAAGATTGGCATTGTCATTTCA
  CCTCTCATTTCCTTAATGGAAGACCAAGTCCTCCAGCTTGAGCTGTCCAATGTTCCAGCCTGTTTACTTG
  GATCTGCACAGTCAAAAAATATTCTAGGAGATGTTAAATTAGGCAAATATAGGGTCATCTACATAACTCC
  AGAGTTCTGTTCTGGTAACTTGGATCTACTCCAGCAACTTGACTCTAGTATTGGCATCACTCTCATTGCT
  GTGGATGAGGCTCACTGCATTTCAGAGTGGGGCCATGATTTCAGAAGTTCATTCAGGATGCTGGGCTCTC
  TTAAAACAGCGCTCCCATTGGTTCCAGTCATTGCACTCTCCGCTACTGCAAGCTCTTCCATCCGGGAAGA
  CATTATAAGCTGCTTAAACCTGAAAGACCCTCAGATCACCTGCACTGGATTTGATCGGCCAAATCTGTAC
  TTAGAAGTTGGACGGAAAACAGGGAACATCCTTCAGGATCTAAAGCCGTTTCTCGTCCGAAAGGCAAGTT
  CTGCCTGGGAATTTGAAGGTCCAACCATCATCTATTGTCCTTCGAGAAAAATGACAGAACAAGTTACTGC
  TGAACTTGGGAAACTGAACTTAGCCTGCAGAACATACCACGCTGGCATGAAAATTAGCGAAAGGAAGGAC
  GTTCATCATAGGTTCCTGAGAGATGAAATTCAGTGTGTTGTAGCTACTGTAGCTTTTGGAATGGGCATTA
  ATAAAGCTGACATTCGCAAAGTTATTCATTATGGTGCGCCTAAGGAAATGGAATCCTATTACCAGGAAAT
  TGGTAGAGCTGGCCGGGATGGACTTCAGAGTTCCTGTCACTTGCTCTGGGCTCCAGCAGACTTTAACACA
  TCCAGGAATCTCCTTATTGAGATTCACGATGAAAAGTTCCGGTTATATAAATTAAAGATGATGGTAAAGA
  TGGAAAAATACCTTCACTCCAGTCAGTGTAGGCGACGAATCATCTTGTCCCATTTTGAGGACAAATGTCT
  GCAGAAGGCCTCCTTGGACATTATGGGAACTGAAAAATGCTGTGATAATTGCAGGCCCAGGCTGAATCAT
  TGCCTTACTGCTAACAACTCAGAGGACGCATCCCAAGACTTTGGGCCACAAGCATTCCAGCTACTGTCTG
  CTGTGGACATCCTGCAGGAGAAATTTGGAATTGGGATTCCGATCTTATTTCTCCGAGGATCTAATTCTCA
  GCGTCTTCCTGATAAATATCGGGGTCACAGGCTCTTTGGTGCTGGAAAGGAGCAAGCAGAAAGTTGGTGG
  AAGACCCTTTCTCACCATCTCATAGCTGAAGGATTCTTGGTAGAAGTTCCCAAGGAAAACAAATATATAA
  AGACATGTTCCCTCACAAAAAAGGGTAGAAAGTGGCTTGGAGAAGCCAGTTCGCAGTCTCCTCCGAGCCT
  TCTCCTTCAAGCTAATGAAGAGATGTTTCCAAGGAAAGTTCTGCTACCAAGTTCTAATCCTGTATCTCCA
  GAAACGACGCAACATTCCTCTAATCAAAACCCAGCTGGATTAACTACCAAGCAGTCTAATTTGGAGAGAA
  CGCATTCTTACAAAGTGCCTGAGAAAGTTTCTTCTGGGACTAACATTCCTAAAAAAAGTGCCGTGATGCC
  GTCACCAGGAACATCTTCCAGCCCCTTAGAACCTGCCATCTCAGCCCAAGAGCTGGACGCTCGGACTGGG
  CTATATGCCAGGCTGGTGGAAGCAAGGCAGAAACACGCTAATAAGATGGATGTACCTCCAGCTATTTTAG
  CAACAAACAAGGTTCTGCTGGACATGGCTAAAATGAGACCGACTACTGTTGAAAACATGAAACAGATCGA
  CGGTGTCTCTGAAGGCAAAGCTGCTCTGTTGGCCCCTCTGTTGGAAGTCATCAAACATTTCTGTCAAGTA
  ACTAGTGTTCAGACAGACCTCCTTTCCAGTGCCAAACCTCACAAGGAACAGGAGAAAAGTCAGGAGATGG
  AAAAGAAAGACTGCTCACTCCCCCAGTCTGTGGCCGTCACATACACTCTATTCCAGGAAAAGAAAATGCC
  CTTACACAGCATAGCTGAGAACAGGCTCCTGCCTCTCACAGCAGCCGGCATGCACTTAGCCCAGGCGGTG
  AAAGCCGGCTACCCCCTGGATATGGAGCGAGCTGGCCTGACCCCAGAGACTTGGAAGATTATTATGGATG
  TCATCCGAAACCCTCCCATCAACTCAGATATGTATAAAGTTAAACTCATCAGAATGTTAGTTCCTGAAAA
  CTTAGACACGTACCTCATCCACATGGCGATTGAGATTCTTCAGAGTGGTTCCGACAGCAGAACCCAGCCT
  CCTTGTGATTCCAGCAGGAAGAGGCGTTTCCCCAGCTCTGCAGAGAGTTGTGAGAGCTGTAAGGAGAGCA
  AAGAGGCGGTCACCGAGACCAAGGCATCATCTTCAGAGTCAAAGAGAAAATTACCCGAGTGGTTTGCCAA
  AGGAAATGTGCCCTCAGCTGATACCGGCAGCTCATCATCAATGGCCAAGACCAAAAAGAAAGGTCTCTTT
  AGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011721
• Insert Size: 4206 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011721.4, NP_035851.3
• RefSeq Size: 6383 bp
• RefSeq ORF: 4206 bp
• Locus ID: 22427
• UniProt ID: O09053
• Cytogenetics: 8 20.3 cM
• Gene Summary: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.