Tdrd9 (NM_029056) Mouse Untagged Clone

CAT#: MC224372

Tdrd9 (untagged) - Mouse tudor domain containing 9 (Tdrd9), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tdrd9
• Synonyms: 4930441E05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224372 representing NM_029056
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCGCAAGCTCACGGTCGACCAGATAAATGACTGGTTCACCATCGGCAAGACGGTGACCAATGTGG
  AGCTTCTGGGCTTGCCACCCGCCTTCCCTGCGGAGGCGCCCCGGGAGGAGGTACAGCGCTCGGAGGAGGT
  CCCCAATGAGGATCCTACGGCCCAGGCCCAGGTTCCAGTTAAGGCCACAGCACCGGCCAGACCAGCTTCA
  ACCTCCGGGAGGTCACTCAGTCAGAGGAGCTCAGAAATGGAGTATATTAACAAATACAGACAACTTGAAG
  AACAAGAACTTGATATTTATGGCCAGGATCAGCCACCCAGTGGACCAGGTCTGAGGTCACCATTGGCTAA
  GTTAAGTAATGTGGCATGCATCCCAGAGACAACTTATAAATATCCTGATTTGCCTATAAATCGGTGTAAA
  GAAGAGGTCATTTCCTTGATAGAAAGCAATTCTGTGGTGATCATACACGGTGCCACAGGAAGCGGCAAGA
  GCACACAGCTCCCACAGTACGTGCTAGACCACTACACCCAGCGCTCTGCCTTCTGCAACATCGTAGTCAC
  CCAGCCGCGGAAGATAGGGGCCAGCAGCATTGCCAGGTGGATCAGTAAGGAGCGTTCCTGGACGCTGGGT
  GGCTTGGTAGGCTATCAGGTAGGGCTGGAGAAAATCGCAACAGAAGACACTAGGTTAATTTACATGACCA
  CTGGAGTCCTGCTTCAGAAAATTGTAAGTGCCAAGAGCCTGATGGAGTTCACGCACATCTTCATTGACGA
  GGTGCATGAACGGACCGAGGAGATGGATTTCCTGCTCTTGGTCGTCCGTAAACTCTTGAGGACAAATTCA
  CGGTTTGTGAAGGTGGTCCTCATGTCTGCCACCATCAACTGCAAGCAGTTTGCAGACTACTTTGCTGTTC
  CCGTTCAGAACAAGATGAACCCGGCATATGTTTTTGAAGTGGAAGGCAAGCCCCACGCTATTGAAGAGTA
  TTACCTTAATGACTTGGGGCACATTTATCATAGTGGGCTTCCCTACCGTCTGGAGGAGCCAGTGATAACA
  AAGGATGTCTATGAAGTTGCTGTCTCTCTCATTCAGATGTTCGATGACCTGGACATGAAAGAGAGCGGGA
  ACAAGACCTGGTCCGGGGCGCAGTTTGTGTCGGAGCGGAGCAGCGTGCTGGTGTTCCTGCCAGGTCTGGG
  TGAGATCAATTACATGCATGAGCTCCTCACAAACATGATTCACAAAAGGTTACAGGTCTATCCACTCCAT
  TCCAGTGTGACTTTAGAAGAACAGAATAACGTATTTTTAAGTCCAGTCCCTGGGTACAGAAAGATTATTC
  TGTCAACCAACATCGCAGAGAGTTCTGTCACAGTTCCTGATGTCAAATATGTTATAGATTTCTGTCTGAC
  CAGAACTCTGGTTTGTGATGAAGATACAAATTATCAGAGTCTGCGACTAAGCTGGGCTTCCAAGACCAGC
  TGTGACCAGAGAAAAGGCCGTGCTGGACGAGTGTCTAAAGGATACTGCTACAGACTGATCCCCAGGGACT
  TCTGGGACAGTGCTATCCCTGACCACGTTGTTCCTGAGATGCTGCGCTGTCCGCTGGGAAGCACAATATT
  GAAGGTGAAGTTGCTTGACATGGGTGAACCGAGAGCTCTGCTGGCCACCGCCCTGTCTCCGCCCAGCCTG
  AGTGACATTGAGCGCACCATTCTTCTGTTAAAGGAGGTTGGTGCACTGGCGGTCAGTGGGCAGAGGGAGG
  ACGAGAACCCCCACGATGGCGAGTTGACCTTCCTGGGACGAGTGCTGGCACAGCTCCCTGTCAGCCAGCA
  GCTGGGTAAACTCGTTGTGCTGGGACATGTGTTTGGGTGCCTGGATGAGTGTCTCATCATAGCGGCAGCT
  CTTTCTCTGAAGAATTTTTTCACGATGCCTTTTCGGCAGCATCTGGATGGATATAGGAACAAAGTGCACT
  TCTCAGGTAGCAGCAGGAGTGACTGCCTGGCACTTGTGGAGGCGTTCAGGGCCTGGCAGGCTTGCAGACA
  GCGAGGAGAGCTGCGGCGTCCCAAGGATGAGCTTGACTGGGGACGGCTAAATTACATTCAAATAAAGAGA
  ATCAGAGAGGTGGCTGAATTATATGAAGAATTGAAGAATAGAATCTCACAGTTCAACATGTTTGTGGGTC
  CTCACCATCCCGTCTTGGATCAAGAATATCCTTATAAGCAGCGCTTCATCCTGCAGGTTGTGCTGGCAGG
  CGCTTTTTATCCAAACTACTTCACTTTTGGGCAGCCAGATGAGGAGATGGCAGTGAGGGAACTGGCTGGC
  AAAGACCCCAAGACAACAGTCGTGCTGAAACACATCCCTCCCTATGGATTTCTTTACTACAAGCAACTGC
  AGTCTCTCTTTAGACAGTGTGGTCAAGTCAAGTCCATTGTATTTGATGGTGCAAAAGCATTTGTGGAGTT
  TTCACGGAATCCAACAGAAAGATTTAAGACCCTCCCTGCTGTGAACTTGGCAGTCAAGATGTCCCAGTTG
  AAAGTGTCCCTAGAGCTCAGTGTCCATGCTGCAGAGGAGATCGAGGGGAAGGTGCAAGGCGGCTCGGTGT
  CAAAGCTCAGGAACACAAGGGTGAACGTGGACTTCCAGAAGCAGACGGTGGATCCCATGCAGGTGTCGTT
  CAACACCTTGGACAGACCCCGCACCGTTGCAGACCTCCTCCTGACAATCGATGTCACTGAGGTGGTAGAA
  GTGGGGCACTTCTGGGGGTACAGGATCGACGAAAGGAATGCAGAGCTGCTGAAACAACTGACTGCTGAGA
  TAAACCGCCTAGAGCTGGTCCCTCTGCCTATCCACCCACATCCAGACTTGGTCTGTCTGGCACCTTTTAC
  TGATTACAACAAGGAAAGCTACTTTAGAGCTCAAATTCTTTATGTTTCTGGAAATTCCGCTGAGGTATTC
  TTTGTAGACTATGGGAATAGGTCTCATGTAGACCTAGATCTCTTGAGGGAGATCCCCTGTCAGTTTTTGG
  AGCTTCCATTTCAGGCTTTGGAATTCAAGATTTGCAAAATGCGACCATCAGCAAAGTCTCTCATCTGTGG
  CGAGCACTGGAGTGGGGGTGCCCATGGGCGCTTTGCTGCCTTGGTCGGCGGCTGTCCTCTCCTGGTGAAG
  GTGTTCTCCATTGTACACAGTGTTCTGCATGTGGACGTGTACCGCTACTCGGGTGCCCAGGATGCTGTCA
  ACGTCAGAGATGTTCTCATCAGGGAGGGCTATGCTGAGCTCGCAGAGGAGTCCTATGAGTCAAAACAAAG
  CTATGAAGTCCTCAAGGGTTTCTTTGCTAAGTCAGTGGACACCATGCCGGACGGCTCTGTCTCCTCTCCT
  CTGAAGGACGATGAGAAGCATCTCCTACGGATTCTGTTGGAGAGCTTTGCCTCCAACCGGCTAGGCGCTC
  CGAACTGCAAGGCTGTTCTCCATGGGCCCTTTAACCCTTATGAGCTGAAGTGTCATAGTCTGACCAGAAT
  ATCCAAGTTCAGATGTGTGTGGATTGAGAAGGAGAGCATCAATTCTGTCGTCATCAGTGACAGCCCTGCC
  GACCTGCACCAGAGGATGCTGGTTGCAGCTTCACTCTCAGTTAACGAGACCGGCTCCACCATGCTCCTGA
  GAGAGACCTCCCTGATGCCGCACATCCCAGGCCTCCCAGCGCTCCTCAGCATGCTGTTCGCACCAGTGAT
  GGAGCTGAGAGTTGACCGTGAGGGAAAATGTTACACTGGGGTCCTTTGTGGTTTGGGATGGAATTCAGCT
  ACAGAGGCCCCTATCCTGCCAGAGCATGACATCGAGCTGGCCTTTGATGTGCGCTTAAACGTGGAGGACA
  TCGTTGAGATTAATATTCTCAGGGCTGCTATCAACAAGCTAGTGTGTGATGGGCCAAATGGATCCAAGTA
  TCTTGGGCCTGAAAGAATCGCTCAGTTGCAGGAGAATGCACGCCAGAAACTTCTAGGCTTGTTCTGTCGG
  TTGAAACCTCGAGAGAAGATTACTCCTCAGTGGCACGAGAAACCCTACGAGTGGAACCAGGTGGATCCAA
  GGCTGATCATGGAGCAAGCTGAGCCAGAGGGCAGCCCAGGAAAGAGCACGTCACTGTACCAGCTGCACAC
  GCCTGTGGTGCTCAGCCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029056
• Insert Size: 4152 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029056.1, NP_083332.1
• RefSeq Size: 4809 bp
• RefSeq ORF: 4152 bp
• Locus ID: 74691
• UniProt ID: Q14BI7
• Cytogenetics: 12 F1
• Gene Summary: ATP-binding RNA helicase which plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:20059948, PubMed:28633017). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20059948, PubMed:28633017). Acts downstream of piRNA biogenesis: exclusively required for transposon silencing in the nucleus, suggesting that it acts as a nuclear effector in the nucleus together with PIWIL4 (PubMed:28633017).[UniProtKB/Swiss-Prot Function]