Met (NM_008591) Mouse Untagged Clone

CAT#: MC224369

Met (untagged) - Mouse met proto-oncogene (Met), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Met
• Synonyms: AI838057; c-Met; HGF; HGFR; Par4
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224369 representing NM_008591
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTCCCACCGTGCTGGCACCTGGCATTCTGGTGCTGCTGTTGTCCTTGGTGCAGAGGAGCCATG
  GGGAGTGCAAGGAGGCCCTAGTGAAGTCTGAGATGAACGTGAACATGAAGTATCAGCTCCCCAACTTCAC
  GGCAGAAACCCCCATCCAGAATGTCGTCCTACACGGCCATCATATTTATCTCGGAGCCACAAACTACATT
  TATGTTTTAAATGACAAAGACCTTCAGAAGGTATCCGAATTCAAGACCGGGCCCGTGTTGGAACACCCAG
  ATTGTTTACCTTGTCGGGACTGCAGCAGCAAAGCCAATTCATCAGGAGGGGTTTGGAAAGACAACATCAA
  CATGGCTCTGCTTGTTGACACATACTATGATGATCAACTCATTAGCTGTGGCAGTGTCAACAGAGGGACT
  TGCCAGCGGCATGTCCTTCCTCCTGACAATTCTGCTGACATCCAGTCTGAGGTCCACTGCATGTTCTCCC
  CAGAAGAGGAGTCAGGGCAGTGTCCTGACTGTGTAGTGAGTGCCCTCGGAGCCAAAGTCCTCCTGTCGGA
  AAAGGACCGGTTCATCAATTTCTTTGTGGGGAATACGATCAATTCCTCCTATCCTCCTGGTTATTCACTG
  CATTCGATATCGGTGAGACGGCTGAAGGAAACCCAAGATGGTTTTAAGTTTTTGACAGACCAGTCCTATA
  TTGATGTCTTACCAGAATTCCAAGATTCCTACCCCATAAAGTACATACATGCCTTCGAAAGCAACCATTT
  TATTTACTTTCTGACTGTCCAAAAGGAAACTCTAGATGCTCAGACTTTTCATACAAGAATAATCAGGTTC
  TGTTCCGTAGACTCTGGGTTGCACTCCTACATGGAAATGCCCCTGGAATGCATCCTGACAGAAAAAAGAA
  GGAAGAGATCCACAAGGGAAGAAGTGTTTAATATCCTCCAAGCCGCGTATGTCAGTAAACCAGGGGCCAA
  TCTTGCTAAGCAAATAGGAGCTAGCCCTTCTGATGACATTCTCTTCGGGGTGTTTGCACAAAGCAAGCCA
  GATTCTGCTGAACCTGTGAATCGATCAGCAGTCTGTGCATTCCCCATCAAATATGTCAATGACTTCTTCA
  ACAAGATTGTCAACAAAAACAACGTGAGATGTCTCCAGCATTTTTACGGACCCAACCATGAGCACTGTTT
  CAATAGGACCCTGCTGAGAAACTCTTCCGGCTGTGAAGCGCGCAGTGACGAGTATCGGACAGAGTTTACC
  ACGGCTTTGCAGCGCGTCGACTTATTCATGGGCCGGCTTAACCAAGTGCTCCTGACATCCATCTCCACCT
  TCATCAAAGGTGACCTCACCATTGCTAATCTAGGGACGTCAGAAGGTCGCTTCATGCAGGTGGTGCTCTC
  TCGAACAGCACACCTCACTCCTCATGTGAACTTCCTCCTGGACTCCCATCCTGTATCTCCAGAAGTTATT
  GTTGAGCATCCATCAAATCAAAATGGCTATACATTGGTTGTCACAGGAAAGAAGATCACCAAGATTCCAT
  TGAATGGCCTGGGCTGTGGACATTTCCAATCCTGCAGTCAGTGCCTCTCTGCCCCTTACTTTATACAGTG
  TGGCTGGTGCCACAATCAATGTGTGCGTTTTGATGAATGCCCCAGCGGTACATGGACTCAAGAGATCTGT
  CTGCCGGCGGTTTATAAGGTGTTCCCCACCAGCGCGCCCCTTGAAGGAGGAACAGTGTTGACCATATGTG
  GCTGGGACTTTGGATTCAGGAAGAATAATAAATTTGATTTAAGGAAAACCAAAGTTCTGCTTGGCAACGA
  GAGCTGTACCTTGACCTTAAGCGAGAGCACGACAAATACGTTGAAATGCACAGTTGGTCCCGCGATGAGT
  GAGCACTTCAATGTGTCTGTAATTATCTCAAACAGTCGAGAGACGACGCAATACAGTGCATTCTCCTATG
  TAGATCCTGTAATAACAAGCATTTCTCCGAGGTACGGCCCTCAGGCTGGAGGCACCTTACTCACTCTTAC
  TGGGAAATACCTCAACAGTGGCAATTCTAGACACATTTCAATTGGAGGGAAAACATGTACTTTAAAAAGT
  GTATCAGATAGTATTCTTGAATGCTACACCCCAGCCCAAACTACCTCTGATGAGTTTCCTGTGAAATTGA
  AGATTGACTTGGCTAACCGAGAGACCAGCAGCTTCAGTTACCGGGAAGACCCCGTTGTCTATGAAATCCA
  CCCGACCAAATCTTTTATTAGTGGTGGAAGCACAATAACGGGTATTGGGAAGACCCTGAACTCGGTTAGC
  CTCCCAAAGCTGGTAATAGATGTGCATGAAGTGGGTGTGAACTACACAGTGGCATGTCAGCATCGCTCAA
  ATTCAGAGATCATCTGCTGCACTACTCCTTCACTGAAACAGCTGGGCCTGCAACTCCCCCTGAAGACCAA
  AGCCTTCTTCCTGTTAGACGGGATTCTTTCCAAACACTTTGATCTCACTTATGTGCATAATCCTGTGTTT
  GAGCCTTTTGAAAAGCCAGTAATGATCTCAATAGGCAATGAAAATGTAGTGGAAATTAAGGGAAACAATA
  TTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTTGGAAATCAGAGCTGCGAGAGTCTCCACTGGCA
  CTCTGGAGCTGTGTTGTGTACAGTCCCCAGTGACCTGCTCAAACTGAACAGCGAGCTAAATATAGAGTGG
  AAGCAAGCAGTCTCTTCAACTGTTCTTGGAAAAGTGATCGTTCAACCGGATCAGAATTTTGCAGGATTGA
  TCATTGGTGCGGTCTCAATATCAGTAGTAGTTTTGTTATTATCCGGGCTCTTCCTGTGGATGAGAAAGAG
  AAAGCATAAAGATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCCTCATTTGGATAGG
  CTTGTAAGTGCCCGAAGTGTAAGTCCAACTACAGAGATGGTTTCAAATGAGTCTGTAGACTACAGAGCTA
  CTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCATGCAGACAAGTGCAATACCCTCTGAC
  AGACCTGTCCCCTATCCTGACAAGTGGAGACTCTGATATATCCAGCCCATTACTACAAAATACTGTTCAC
  ATTGACCTCAGTGCTCTAAATCCAGAGCTGGTCCAAGCAGTTCAGCACGTAGTGATTGGACCCAGCAGCC
  TGATTGTGCATTTCAATGAAGTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATGGGACTTTGCTGGA
  CAATGACGGAAAGAAAATTCACTGTGCTGTGAAATCCTTAAATAGAATCACAGATATAGAAGAGGTCTCC
  CAGTTTCTGACTGAGGGAATCATCATGAAAGACTTCAGCCATCCCAATGTTCTCTCACTCTTGGGAATCT
  GCCTGAGGAGTGAAGGGTCTCCTCTGGTGGTCCTGCCCTATATGAAGCATGGAGATCTGCGAAATTTCAT
  TCGAAACGAGACTCATAATCCAACTGTGAAAGATCTTATAGGATTTGGCCTTCAAGTAGCCAAAGGCATG
  AAATATCTTGCCAGCAAAAAGTTTGTCCACAGAGACTTAGCTGCAAGAAACTGCATGTTGGATGAAAAAT
  TCACTGTCAAGGTTGCTGATTTCGGTCTTGCCAGAGACATGTACGATAAAGAGTACTATAGTGTCCACAA
  CAAGACGGGTGCCAAGCTACCAGTAAAGTGGATGGCTTTAGAGAGTCTGCAAACGCAGAAGTTCACCACC
  AAGTCAGATGTGTGGTCCTTTGGTGTGCTCCTCTGGGAGCTCATGACGAGAGGAGCCCCTCCTTATCCCG
  ACGTGAACACATTTGATATCACTATCTACCTGTTGCAAGGCAGAAGACTCTTGCAACCAGAATACTGTCC
  AGACGCCTTGTACGAAGTGATGCTAAAATGCTGGCACCCCAAAGCGGAAATGCGCCCGTCCTTTTCCGAA
  CTGGTCTCCAGGATATCCTCAATCTTCTCCACGTTCATTGGGGAACACTACGTCCACGTGAACGCTACTT
  ATGTGAATGTAAAATGTGTTGCTCCATATCCTTCTCTGTTGCCATCCCAAGACAACATTGATGGCGAGGG
  GAACACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008591
• Insert Size: 4140 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008591.2, NP_032617.2
• RefSeq Size: 6652 bp
• RefSeq ORF: 4140 bp
• Locus ID: 17295
• Cytogenetics: 6 7.83 cM