Met (NM_008591) Mouse Tagged ORF Clone

CAT#: MR226566

Met (Myc-DDK-tagged) - Mouse met proto-oncogene (Met)

Product Images

Circular map for MR226566

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Met
• Synonyms: AI838057; c-Met; HGF; HGFR; Par4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226566 representing NM_008591
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTCCCACCGTGCTGGCACCTGGCATTCTGGTGCTGCTGTTGTCCTTGGTGCAGAGGAGCCATG
  GGGAGTGCAAGGAGGCCCTAGTGAAGTCTGAGATGAACGTGAACATGAAGTATCAGCTCCCCAACTTCAC
  GGCAGAAACCCCCATCCAGAATGTCGTCCTACACGGCCATCATATTTATCTCGGAGCCACAAACTACATT
  TATGTTTTAAATGACAAAGACCTTCAGAAGGTATCCGAATTCAAGACCGGGCCCGTGTTGGAACACCCAG
  ATTGTTTACCTTGTCGGGACTGCAGCAGCAAAGCCAATTCATCAGGAGGGGTTTGGAAAGACAACATCAA
  CATGGCTCTGCTTGTTGACACATACTATGATGATCAACTCATTAGCTGTGGCAGTGTCAACAGAGGGACT
  TGCCAGCGGCATGTCCTTCCTCCTGACAATTCTGCTGACATCCAGTCTGAGGTCCACTGCATGTTCTCCC
  CAGAAGAGGAGTCAGGGCAGTGTCCTGACTGTGTAGTGAGTGCCCTCGGAGCCAAAGTCCTCCTGTCGGA
  AAAGGACCGGTTCATCAATTTCTTTGTGGGGAATACGATCAATTCCTCCTATCCTCCTGGTTATTCACTG
  CATTCGATATCGGTGAGACGGCTGAAGGAAACCCAAGATGGTTTTAAGTTTTTGACAGACCAGTCCTATA
  TTGATGTCTTACCAGAATTCCAAGATTCCTACCCCATAAAGTACATACATGCCTTCGAAAGCAACCATTT
  TATTTACTTTCTGACTGTCCAAAAGGAAACTCTAGATGCTCAGACTTTTCATACAAGAATAATCAGGTTC
  TGTTCCGTAGACTCTGGGTTGCACTCCTACATGGAAATGCCCCTGGAATGCATCCTGACAGAAAAAAGAA
  GGAAGAGATCCACAAGGGAAGAAGTGTTTAATATCCTCCAAGCCGCGTATGTCAGTAAACCAGGGGCCAA
  TCTTGCTAAGCAAATAGGAGCTAGCCCTTCTGATGACATTCTCTTCGGGGTGTTTGCACAAAGCAAGCCA
  GATTCTGCTGAACCTGTGAATCGATCAGCAGTCTGTGCATTCCCCATCAAATATGTCAATGACTTCTTCA
  ACAAGATTGTCAACAAAAACAACGTGAGATGTCTCCAGCATTTTTACGGACCCAACCATGAGCACTGTTT
  CAATAGGACCCTGCTGAGAAACTCTTCCGGCTGTGAAGCGCGCAGTGACGAGTATCGGACAGAGTTTACC
  ACGGCTTTGCAGCGCGTCGACTTATTCATGGGCCGGCTTAACCAAGTGCTCCTGACATCCATCTCCACCT
  TCATCAAAGGTGACCTCACCATTGCTAATCTAGGGACGTCAGAAGGTCGCTTCATGCAGGTGGTGCTCTC
  TCGAACAGCACACCTCACTCCTCATGTGAACTTCCTCCTGGACTCCCATCCTGTATCTCCAGAAGTTATT
  GTTGAGCATCCATCAAATCAAAATGGCTATACATTGGTTGTCACAGGAAAGAAGATCACCAAGATTCCAT
  TGAATGGCCTGGGCTGTGGACATTTCCAATCCTGCAGTCAGTGCCTCTCTGCCCCTTACTTTATACAGTG
  TGGCTGGTGCCACAATCAATGTGTGCGTTTTGATGAATGCCCCAGCGGTACATGGACTCAAGAGATCTGT
  CTGCCGGCGGTTTATAAGGTGTTCCCCACCAGCGCGCCCCTTGAAGGAGGAACAGTGTTGACCATATGTG
  GCTGGGACTTTGGATTCAGGAAGAATAATAAATTTGATTTAAGGAAAACCAAAGTTCTGCTTGGCAACGA
  GAGCTGTACCTTGACCTTAAGCGAGAGCACGACAAATACGTTGAAATGCACAGTTGGTCCCGCGATGAGT
  GAGCACTTCAATGTGTCTGTAATTATCTCAAACAGTCGAGAGACGACGCAATACAGTGCATTCTCCTATG
  TAGATCCTGTAATAACAAGCATTTCTCCGAGGTACGGCCCTCAGGCTGGAGGCACCTTACTCACTCTTAC
  TGGGAAATACCTCAACAGTGGCAATTCTAGACACATTTCAATTGGAGGGAAAACATGTACTTTAAAAAGT
  GTATCAGATAGTATTCTTGAATGCTACACCCCAGCCCAAACTACCTCTGATGAGTTTCCTGTGAAATTGA
  AGATTGACTTGGCTAACCGAGAGACCAGCAGCTTCAGTTACCGGGAAGACCCCGTTGTCTATGAAATCCA
  CCCGACCAAATCTTTTATTAGTGGTGGAAGCACAATAACGGGTATTGGGAAGACCCTGAACTCGGTTAGC
  CTCCCAAAGCTGGTAATAGATGTGCATGAAGTGGGTGTGAACTACACAGTGGCATGTCAGCATCGCTCAA
  ATTCAGAGATCATCTGCTGCACTACTCCTTCACTGAAACAGCTGGGCCTGCAACTCCCCCTGAAGACCAA
  AGCCTTCTTCCTGTTAGACGGGATTCTTTCCAAACACTTTGATCTCACTTATGTGCATAATCCTGTGTTT
  GAGCCTTTTGAAAAGCCAGTAATGATCTCAATAGGCAATGAAAATGTAGTGGAAATTAAGGGAAACAATA
  TTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTTGGAAATCAGAGCTGCGAGAGTCTCCACTGGCA
  CTCTGGAGCTGTGTTGTGTACAGTCCCCAGTGACCTGCTCAAACTGAACAGCGAGCTAAATATAGAGTGG
  AAGCAAGCAGTCTCTTCAACTGTTCTTGGAAAAGTGATCGTTCAACCGGATCAGAATTTTGCAGGATTGA
  TCATTGGTGCGGTCTCAATATCAGTAGTAGTTTTGTTATTATCCGGGCTCTTCCTGTGGATGAGAAAGAG
  AAAGCATAAAGATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCCTCATTTGGATAGG
  CTTGTAAGTGCCCGAAGTGTAAGTCCAACTACAGAGATGGTTTCAAATGAGTCTGTAGACTACAGAGCTA
  CTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCATGCAGACAAGTGCAATACCCTCTGAC
  AGACCTGTCCCCTATCCTGACAAGTGGAGACTCTGATATATCCAGCCCATTACTACAAAATACTGTTCAC
  ATTGACCTCAGTGCTCTAAATCCAGAGCTGGTCCAAGCAGTTCAGCACGTAGTGATTGGACCCAGCAGCC
  TGATTGTGCATTTCAATGAAGTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATGGGACTTTGCTGGA
  CAATGACGGAAAGAAAATTCACTGTGCTGTGAAATCCTTAAATAGAATCACAGATATAGAAGAGGTCTCC
  CAGTTTCTGACTGAGGGAATCATCATGAAAGACTTCAGCCATCCCAATGTTCTCTCACTCTTGGGAATCT
  GCCTGAGGAGTGAAGGGTCTCCTCTGGTGGTCCTGCCCTATATGAAGCATGGAGATCTGCGAAATTTCAT
  TCGAAACGAGACTCATAATCCAACTGTGAAAGATCTTATAGGATTTGGCCTTCAAGTAGCCAAAGGCATG
  AAATATCTTGCCAGCAAAAAGTTTGTCCACAGAGACTTAGCTGCAAGAAACTGCATGTTGGATGAAAAAT
  TCACTGTCAAGGTTGCTGATTTCGGTCTTGCCAGAGACATGTACGATAAAGAGTACTATAGTGTCCACAA
  CAAGACGGGTGCCAAGCTACCAGTAAAGTGGATGGCTTTAGAGAGTCTGCAAACGCAGAAGTTCACCACC
  AAGTCAGATGTGTGGTCCTTTGGTGTGCTCCTCTGGGAGCTCATGACGAGAGGAGCCCCTCCTTATCCCG
  ACGTGAACACATTTGATATCACTATCTACCTGTTGCAAGGCAGAAGACTCTTGCAACCAGAATACTGTCC
  AGACGCCTTGTACGAAGTGATGCTAAAATGCTGGCACCCCAAAGCGGAAATGCGCCCGTCCTTTTCCGAA
  CTGGTCTCCAGGATATCCTCAATCTTCTCCACGTTCATTGGGGAACACTACGTCCACGTGAACGCTACTT
  ATGTGAATGTAAAATGTGTTGCTCCATATCCTTCTCTGTTGCCATCCCAAGACAACATTGATGGCGAGGG
  GAACACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226566 representing NM_008591
  Red=Cloning site Green=Tags(s)
  
  MKAPTVLAPGILVLLLSLVQRSHGECKEALVKSEMNVNMKYQLPNFTAETPIQNVVLHGHHIYLGATNYI
  YVLNDKDLQKVSEFKTGPVLEHPDCLPCRDCSSKANSSGGVWKDNINMALLVDTYYDDQLISCGSVNRGT
  CQRHVLPPDNSADIQSEVHCMFSPEEESGQCPDCVVSALGAKVLLSEKDRFINFFVGNTINSSYPPGYSL
  HSISVRRLKETQDGFKFLTDQSYIDVLPEFQDSYPIKYIHAFESNHFIYFLTVQKETLDAQTFHTRIIRF
  CSVDSGLHSYMEMPLECILTEKRRKRSTREEVFNILQAAYVSKPGANLAKQIGASPSDDILFGVFAQSKP
  DSAEPVNRSAVCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARSDEYRTEFT
  TALQRVDLFMGRLNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVLSRTAHLTPHVNFLLDSHPVSPEVI
  VEHPSNQNGYTLVVTGKKITKIPLNGLGCGHFQSCSQCLSAPYFIQCGWCHNQCVRFDECPSGTWTQEIC
  LPAVYKVFPTSAPLEGGTVLTICGWDFGFRKNNKFDLRKTKVLLGNESCTLTLSESTTNTLKCTVGPAMS
  EHFNVSVIISNSRETTQYSAFSYVDPVITSISPRYGPQAGGTLLTLTGKYLNSGNSRHISIGGKTCTLKS
  VSDSILECYTPAQTTSDEFPVKLKIDLANRETSSFSYREDPVVYEIHPTKSFISGGSTITGIGKTLNSVS
  LPKLVIDVHEVGVNYTVACQHRSNSEIICCTTPSLKQLGLQLPLKTKAFFLLDGILSKHFDLTYVHNPVF
  EPFEKPVMISIGNENVVEIKGNNIDPEAVKGEVLKVGNQSCESLHWHSGAVLCTVPSDLLKLNSELNIEW
  KQAVSSTVLGKVIVQPDQNFAGLIIGAVSISVVVLLLSGLFLWMRKRKHKDLGSELVRYDARVHTPHLDR
  LVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGACRQVQYPLTDLSPILTSGDSDISSPLLQNTVH
  IDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIEEVS
  QFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGM
  KYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTT
  KSDVWSFGVLLWELMTRGAPPYPDVNTFDITIYLLQGRRLLQPEYCPDALYEVMLKCWHPKAEMRPSFSE
  LVSRISSIFSTFIGEHYVHVNATYVNVKCVAPYPSLLPSQDNIDGEGNT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008591
• ORF Size: 4137 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008591.2, NP_032617.2
• RefSeq Size: 6652 bp
• RefSeq ORF: 4140 bp
• Locus ID: 17295
• Cytogenetics: 6 7.83 cM
• MW: 154 kDa