Rims1 (NM_001012623) Mouse Untagged Clone

CAT#: MC224364

Rims1 (untagged) - Mouse regulating synaptic membrane exocytosis 1 (Rims1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rims1
• Synonyms: C030033M19Rik; mKIAA0340; Rab3ip1; Rim; RIM1; RIM1a; RIM1alpha; Serg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224364 representing NM_001012623
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG
  ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA
  GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA
  AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC
  CAAGCAGCGAAGAGGGAGGCCCAGACAGAAACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAACA
  AGTGAGAAAAATCGGAGAGGAAGCCAGGCGTTACCAGGGCGAGCACAAAGATGATGCCCCGACGTGTGGA
  ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG
  CACGCTGTGGAGGCCGAGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT
  ATGTCGAAAGCAACAAGAGATATTAACGAAATCTGGAGCGTGGTTCTTCGGAAGTGGTCCTCAGCAGCCC
  AGTCAAGATGGGACTCTGAGTGACACGGCTACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCTC
  GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTTC
  CCATGGTGCACCACTGGACAGGAACAAAGGGGCGGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG
  CAGGCATCAAGGTCAAGAAGTGAGCCACCAAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG
  GCAAGGGAGGCCAGAAGAGTGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGACCGC
  GGATGAACGGGAGAGGAAGGAGAGGCGGGAAACCCGCAGGCTGGAGAAAGGGCGCTCTCAGGACTACCCG
  GACCGGCTCGAGAAACGCGAGGATGGCAGGGTGGCTGAAGACGAAAAGCAGAGGAAGGAGGAGGAGGGCG
  TGTCCACGCCGGAGTACACGAGCTGCGAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGA
  CTTGGACTACTGGTTGGATCCTGCCACGTGGCATAGCAGGGAAACGTCGCCTATTAGTTCGCATCCTGTA
  ACGTGGCAGCCATCTAAAGAGGGGGACCGATTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGC
  CCAAAGAATCAGGTGCATTATTGGGTCTCAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGTCTTGG
  TGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTGGCAGATGTTGTCGGACACCTAAGAGCAGGGGATGAA
  GTTCTAGAGTGGAATGGTAAACCCCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATTTTAGAAT
  CAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAG
  TTCCCACCCTCCTCTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCT
  GTTATTTCTCCAACCAGCCCTGGAGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGA
  AGCTGTGGTATGATAAGGTGGGGCACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAG
  AGTAGATGGGCGTCCCAGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAGAGTAAA
  AGAAGAACCAAAACGGTAAAGAAACTTCTAGAGCCAAAATGGAATCAAACATTTGTCTACTCACACGTAC
  ATCGTCGAGATTTTCGAGAGCGAATGTTAGAAATTACTGTGTGGGACCAGCCAAGAGTACAGGACGAGGA
  GAGTGAATTTCTAGGAGAGATCCTCATAGAATTGGAAACAGCACTTTTAGATGATGAGCCCCATTGGTAT
  AAACTGCAGACACATGATGAATCTTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATA
  TCCACGGAGAAAGCTCCAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTA
  TGAGGTTGATGATGGTATTGGCGTAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCC
  ACCACGTTAACAGTGCCAGAGCAGCAAAGAACCACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCG
  GCGATGATCAGGGAAGGCCTCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATCCA
  TCCAACACGAAGGTCACGTTCTCCAACCCGACACCATGATGCCTCCCGAAGCCTGGCCGATCACAGATCA
  CGACATGCGGAAAGTCAATATTCGTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAG
  GACGAAGTGCAGAATGCCTACACATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAG
  TACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAA
  AGATCTAGCATCCAAAAACAGTCTAGGAAAGGCACAGCCTCTGATGCAGACAGGACGCACCGACAAGGAA
  GCCCCACCCAGTCACCTCCAGCTGACACATCCTTTGGCAGTCGCCGCGGAAGACAGCTCCCACAGGTGCC
  GGTCCGAAGCGGCAGTATAGAACAAGCGAGCTTAGTAGTGGAGGAACGAACGAGACAGATGAAAATGAAG
  GTACACCGATTTAAGCAGACAACAGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGT
  ACAACATACATAAAGATCAGTACAGAAGCTGTGATAACGCGTCCGCCAAGTCATCAGATAGTGATGTCAG
  TGATGTGTCCGCCATTTCCAGAGCCAGCAGCACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAG
  TCTGAGCGCCCCAGGGGCAGGATCAGTTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAG
  GAAGAGCCATCATCAAGAGCACCAGTGTAAGTGGAGAGATATATACACTGGAGCACAATGACGGCAGCCA
  GTCCGACACGGCCGTGGGTACCGTTGGAGCTGGTGGAAAGAAGCGGAGGTCCAGCCTGAGTGCCAAAGTG
  GTAGCCATTGTGTCTCGAAGAAGCAGAAGCACATCACAGCTCAGCCAGACAGAGTCCGGCCACAAGAAGT
  TAAAAAGCACCATTCAGAGGAGTACGGAAACGGGAATGGCGGCTGAAATGCGGAAGATGGTGAGACAGCC
  CAGCCGGGAGTCCACGGATGGCAGCATCAACAGTTATAGCTCAGAAGGAAACTTAATATTTCCTGGAGTT
  CGCGTGGGACCTGACAGTCAGTTCAGTGATTTCCTTGATGGACTGGGGCCAGCCCAGCTTGTCGGCCGCC
  AAACCCTAGCCACCCCAGCCATGGGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGT
  TGAGGTTATCAGAGCCCGGAGCCTTACACAAAAACCTGGCTCCAAATCTACACCTGCTCCCTATGTGAAA
  GTTTATCTTTTGGAAAATGGAGCCTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATC
  CCTTGTATCAGCAGTCCCTGGTTTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGG
  TGACTATGGAAGAATGGACCACAAATGCTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTG
  TCCAGCATGGTGATTGGATGGTATAAATTGTTCCCTCCATCCTCCCTGGTGGATCCCACACTCACTCCCC
  TGACCCGCCGGGCTTCCCAATCATCACTGGAAAGTTCGTCTGGGCCTCCCTGCATCCGGTCATAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001012623
• Insert Size: 4125 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012623.1, NP_001012641.1
• RefSeq Size: 4125 bp
• RefSeq ORF: 4125 bp
• Locus ID: 116837
• UniProt ID: Q99NE5
• Cytogenetics: 1 A5
• Gene Summary: Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]