Sik3 (NM_027498) Mouse Untagged Clone

CAT#: MC224338

Sik3 (untagged) - Mouse SIK family kinase 3 (Sik3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sik3
• Synonyms: 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; mKIAA0999; Qsk; SIK-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224338 representing NM_027498
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGTTGCCGTGGCCGGAGCAGGGGGAGCCGGGCCGGCCG
  GCCGCCTGCTGCCTCCGCCCGCCGCGGGGCCCCCGGCAGCCCCCGCCGCAGTGCCCCCGGCCGCCGTCCC
  GGCGCGTCCCACAGCCCCGGCCTCCCGCGGGTCGATGGCCGCTCGCATCGGCTACTACGAGATCGACCGC
  ACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTA
  TTAAAATCATAGATAAGAGCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAAT
  GAAGATGCTTTGCCATCCACACATCATCAGACTCTACCAGGTCATGGAGACAGAGCGCATGATTTACCTG
  GTGACAGAATACGCTAGCGGAGGGGAGATATTTGACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGG
  AAGCTCGACGGAAGTTCAAACAGATCGTCACAGCGGTGTATTTTTGTCACTGTCGGAATATCGTTCATCG
  TGATTTAAAAGCCGAAAACTTACTTCTGGACGCCAATCTGAATATCAAAATAGCAGACTTTGGCTTCAGC
  AACCTCTTCACTCCAGGGCAGCTGCTGAAGACGTGGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCT
  TCGAAGGGAAGGAATATGATGGGCCCAAAGTGGACATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGT
  GTGTGGCGCCCTGCCGTTTGATGGGAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTC
  CGCATCCCGTTCTTTATGTCCACAGAGTGTGAGCACTTGATCCGCCACATGCTGGTGTTAGATCCAAATA
  AGCGCCTCTCAATGGAACAGATCTGCAGGCACAAGTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGA
  CAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAGGAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTC
  TTGGCTATGGAAGACATGGGGCTGGACAAGGAGCGCACACTTCAGTCATTAAGGTCAGATGCCTATGACC
  ACTATAGTGCAATCTACAGCCTGCTGTGCGATCGACACAAGAAACATAAAACTCTGCGTCCCGGAGCACT
  TCCCAGCATGCCCCAAGCCATGACTTTCCAGGCACCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCT
  ATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCAACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTG
  TGAACTTGGACAGTGATGAGGGTGAAGAGCCTTCTCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAG
  GCACACGGTGGGAGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTT
  CCTGGAGTAAACCCACAGGGTCCATTCCTACAAGTGGCCCCTAACATGAACTTCACGCACAACCTGTTGC
  CCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGAGTACAAGGAACAGTCTCTGTTACAGCCGCCTACACT
  ACAGCTACTGAACGGAATGGGGCCCCTTGGCCGGAGAGCCTCAGATGGAGGCGCCAACATCCAACTGCAT
  GCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGTCCCCACTTGTCACCATGACACCAGCAGTGCCAGCAG
  TTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAA
  TAGGTCCAAAAGGCACACACTGGCCATGACCAGCCCGACAGCTGAGATCCCACCGGACCTGCAACGGCAG
  CTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGACCAGCATCGCTCCACCT
  ACAAGGACTCCAACACCCTGCATCTCCCTACGGAGCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGC
  TGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAG
  CAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGC
  AGCAGCATATGTTGTACCAGCAGGAGCAGCACCATCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTG
  TCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCCTGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTC
  CAGAGGTTAAGGATTCAACCTTCAAGCCCACCCCCCAACCATCCCAGCAACCATCTCTTCAGACAGCCAA
  GTAACAGTCCTCCCCCGGTCAGCAGTGCCATGATCACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCA
  AGGCTTACCCTCCCATGGTGCAATCTTCCAGCAGCAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCA
  CTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGTCACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTG
  ACATGCTCAGCAACATGGCCGGAACCGCGGCAGGCTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGC
  CAGTCAGATTCAGATACAGCACCGAGCCAGCCTAATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCC
  AAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACAGCTTGAACATGAATCGGTTCTCCCCTGCCAACTACG
  ACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTC
  AACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTGAAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCT
  CCCAGTGCCCAGCAGCAGCCACCCCACTATACCACGTCAGCACTACAGCAGGCCCTGCTATCTCCTACAC
  CGCCAGACTATCCCCGACACCAGCAGGTTCCCCATATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACT
  CACCGGCCACTCGGACATTCGGCTGCCTCCGGCAGAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCAT
  CGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAGAATACCATGAATTGTTCAGGCACATGAACCAAGGGG
  ATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACAGAATATGACAGAGCAACAGGCTTTATCTTATCAAAA
  TGCTGACTCGTACCACCGCCACCACACCAGCCCCCAGCATATCTTACAGATCAGGGCGCAAGATTGTATC
  TCACAGGGTCCCTCGCCCACCCCCACCCATGGCTATGCCCATCAGCCACCACTAATGCATTCGGAGAGTA
  TGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGAGGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAA
  AGGTTGCCACAACAGCCCTCTGCTCTTGTGTACCAGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACT
  GTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCTACGGACACCAGCCAACTGCCACCACGTTCAGTAGAA
  ATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAACTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGA
  GCTGCCGGATCACAACGGCCTTGGGTACCCAGTACGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACG
  CTCCAGAGACACCACACGATCCAGAACAGCGACGATGCCTATGTACAGCTGGATACCTTGCCAGGAATGA
  GCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCGGATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCAT
  GGGCAGCCAACAGTTTCAGGATGAGGAAGATGAAGAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGC
  CTGGGTGATGGCAGCCAGCATCTCAACTCCTCTCGCTATCCAGCTACGTGTGTTACAGACATCATGCTCA
  GCCACAAGCACCCAGAGGTCTCCTTCAGCATGGAGCAAGCCGGCGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027498
• Insert Size: 4110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027498.3, NP_081774.3
• RefSeq Size: 6296 bp
• RefSeq ORF: 4110 bp
• Locus ID: 70661
• UniProt ID: Q6P4S6
• Cytogenetics: 9 A5.2
• Gene Summary: Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).[UniProtKB/Swiss-Prot Function]