Usp47 (NM_133758) Mouse Untagged Clone

CAT#: MC224327

Usp47 (untagged) - Mouse ubiquitin specific peptidase 47 (Usp47), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp47
• Synonyms: 4930502N04Rik; A630020C16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224327 representing NM_133758
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAGGAGATAGAAAATGCTGCTGAAGAACCTAGAGTCT
  TATGTATAATACAAGACACTACTAACTCGAAGACAGTGAGTGAACGGATCACATTAAATTTACCAGCATC
  TACCCCAGTCCGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGATTTG
  ACCAGGGAAAATGGAGTCACTACTGCTGACATGGCACCACTGGATCACACCAGTGACAAGTCTCTTCTTG
  ATGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACCGCCTCAGAT
  GTTGTTGGAGGACTCCAATAACGTGGATGACAGCGTGCATGACAGGTTCATAGGTCCACTGCCAAGAGAA
  GGTTCTGTGGCCTCTACCAATGATTATGTCAGCCAGAACTACTCCTATTCATCAATTTTGAACAAGTCAG
  AAACTGGTTATGTGGGATTAGTAAACCAGGCGATGACGTGCTATTTGAATAGCCTTTTGCAAACACTTTT
  TATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGACTCTGAAGAAGATCCTGTAACG
  AGTATCCCATACCAGCTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAGACCA
  CAGATGTTACAAGGAGCTTTGGATGGGATAGCAGTGAGGCTTGGCAACAGCATGATGTACAAGAACTGTG
  CCGTGTTATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTG
  TACCAAGGCAAGCTGAAAGATTATGTGAGGTGTCTGGAATGCGGTTATGAAGGCTGGAGAATTGACACGT
  ATCTTGATATTCCACTGGTCATCCGACCTTATGGGTCCAGCCAAGCGTTTGCTAGTGTGGAAGAAGCGTT
  GCATGCATTTATTCAACCAGAGATCTTGGATGGCCCAAATCAATATTTTTGTGAACGTTGTAAGAAGAAG
  TGTGATGCTCGGAAGGGCCTTCGGTTTTTGCATTTCCCCTATCTGCTGACCTTACAGTTGAAAAGGTTTG
  ATTTTGATTATACAACCATGCATAGAATTAAACTGAATGATCGAATGTCATTTCCGGAGGAGCTGGATAT
  GAGCACATTTATTGATATTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGATAGTGGAGCAGAA
  AATGAAGGCAGTTGCCACAGCGACCAGATGAGTAATGATTTCTCCACGGATGATGCTGTTGATGAAGGGA
  TATGCCTGGAGAGCAGTAGTGGGAGTGAGAAGATTTCAAAACCTGGCCTGGAAAAGAACTCCCTGATGTA
  TGAGCTCTTCTCAGTGATGGTGCATTCAGGGAGTGCTGCTGGAGGACACTATTATGCTTGCATAAAGTCT
  TTCAGTGACGATCAATGGTACAGTTTCAATGACCAACATGTGAGCAGGATAACACAAGAAGACATTAAGA
  AGACACATGGTGGGTCTTCAGGAAGCAGAGGCTACTACTCCAGTGCTTTTGCAAGCTCAACAAATGCATA
  TATGCTGATATACAGACTGAAGGATCCAACAAGAAATGCAAAGTTTCTAGAAGTGGACGAGTACCCAGAA
  CACATTAAGAATCTGGTGCAGAAGGAGAGAGAGCTGGAAGAGCAGGAAAAGAGGCAACGAGAGATTGAAC
  GCAACACGTGCAAGATAAAATTATTTTGTTTGCATCCTGTGAAACAAGTAATGATGGAAAACAAGCTGGA
  GGTTCATAAGGATAAGACGCTAAAGGAAGCCGTTGAAATGGCTTATAAGATGATGGATTTAGAAGATGTG
  ATTCCCTTGGATTGCTGTCGCCTGGTAAAATATGACGAGTTTCATGATTACCTGGAACGATCGTATGAGG
  GAGAAGAAGATACACCAATGGGGTTGCTTCTAGGAGGAGTCAAGTCCACATACATGTTTGATCTGCTGTT
  GGAAACGAGAAAGCCTGATCAAATTTTTCAGTCTTACAAACCTGGAGAAGTGATGGTGAAAGTTCATGTT
  GTTGATCTGAAAGCAGAGACTGTCGCTGCTCCTGTAACAGTGCGTGCGTATTTAAATCAGACAGTTACAG
  AGTTTAAACAGCTGATCTCAAAGGCCACGCACTTACCTGCAGACTCCATGAGGATAGTGCTGGAGCGCTG
  CTACAATGACCTGCGCCTTCTCAGCATGCCCAGCAAGACCCTGAAGGCTGAGGGCTTCTTCAGAAGTAAC
  AAGGTGTTTGTTGAAAGCTCTGAGACAGTGGATCACCAGGCAGCCTTTACAGACTCCCACTTGTGGAAAC
  TCCTGGATCGACATGCAAATACAATCCGGTTATTTGTTTTGCTGCCTGAACAATCTCCAGGATCATATTC
  GAAAAGGACAGCATACCAGAAAGCTGGAGGTGATTCTGGTAATGTGGACGATGATTGTGAAAGAGTCAAA
  GGACCTGCAGGGAATTTAAAGTCTGTGGATGCTATTCTAGAAGAGAGCACTGAAAAACTGAAAAGCTTGT
  CACTGCAGCAGCAGCAGCAAGATGGGGACAACGGAGACAGCAGCAAAAGCACTGAGACAAGTGACTTTGA
  AAATATTGAATCCCCTCTCAATGAGAGGGGCTCTTCCACCTCTGTGGATAATCGAGAACTTGAACAGCAC
  ATTCAGACTTCTGACCCAGAAAACTTTCAGTCTGAGGAACGCTCGGACTCAGATGTGAATAATGACCGGA
  GTACAAGTTCGGTAGACAGTGATATTCTTAGCTCCAGTCACAGCAGTGACACTTTGTGCAATGCAGACAG
  CGCTCAGATTCCTCTGGCCAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACTAAAGCAAAC
  GAGGGGAAAAAGGAGACCTGGGACACAGCTGAAGAAGACTCTGGGACTGACAGTGAATATGACGAGAGTG
  GCAAGAGCAGGGGGGACATGCAGTACATGTACTTCAAAGCTGACCCCTACACTGCGGATGAAGGCTCTGG
  GGAAGGACACAAATGGTTGATGGTGCATGTTGATAAGAGAATCACCCTGGCAGCTTTCAAACAGCATTTA
  GAACCTTTTGTTGGAGTCCTCTCCTCTCACTTCAAGGTTTTTCGAGTGTACACCAGCAATCAAGAGTTTG
  AGACCGTCCGGTTGAATGAGACCCTTTCATCATTTTCAGATGACAATAAGATCACAATCCGACTGGGGAG
  AGCACTGAAGAAGGGAGAGTACAGAGTTAAAGTGTGCCAGCTCTTAGTCAATGAGCAAGAGCCATGCAAG
  TTTCTGCTAGACGCTGTGTTTGCTAAAGGCATGACTGTGCGGCAATCAAAAGAGGAGCTGATTCCTCAAC
  TCAGGGAGCAGTGTGGCTTAGACCTCAGCATCGACAGGTTTCGTCTAAGGAAGAAAACATGGAAGAATCC
  TGGCACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAAGTTTTC
  CTGGAAGTTCTTGATGGTGTGGAGAAGATGAAGTCCATGTCACAGCTTGCCATCTTAACAAGACGCTGGA
  GGCCTGCAGAGATGAAGTTGGACCCCTTCCAGGAGCTTGTGTTGGAAAGCAATAGTGTTGATGAACTCCG
  AGAGAAGCTAAGTGAAATCAGTGGGATTCCTTTGGAAGACATTGAATTTGCCAAGGGTAGGGGAACCTTC
  CCCTGTGATATTTCTGTCCTTGATATTCATCAAGATTTGGACTGGAATCCTAAAGTCTCTACCCTGAATG
  TTTGGCCTCTTTATATCTGTGATGATGGTGCCGTCATATTTTATAGGGATAGAACAGAAGAAGTGATGGA
  ATTGACAGATGAGCAAAGAAATGAATTGATGAAAAAGGAAAGCAGCCGACTGCAGAAGACGGGACATCGT
  GTGACATACTCCCCTCGCAAAGAGAAAGCACTCAAGATCTACCTGGATGGGGCGCCAAATAAAGATGTGG
  CTCAAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133758
• Insert Size: 4071 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133758.3, NP_598519.2
• RefSeq Size: 5540 bp
• RefSeq ORF: 4071 bp
• Locus ID: 74996
• UniProt ID: Q8BY87
• Cytogenetics: 7 F1
• Gene Summary: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) (By similarity). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1.