Astn2 (NM_207109) Mouse Untagged Clone

CAT#: MC224303

Astn2 (untagged) - Mouse astrotactin 2 (Astn2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Astn2
• Synonyms: 1d8; Astnl; bM452J22.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224303 representing NM_207109
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCCGGCGCCCGGCGCAGCCCGGGCCGCGGCTTGGGGCTTCGGGGGCGGCCGAGGCTCGGCT
  TCCACCCGGGGCCGCCGCCGCCACCGCCGCCGCCGCTGCTGTTGCTGTTTCTGCTCCTGCTGCCGCCGCC
  GCCGTTGCTGGCCGGCGCCACGGCCGCCGCCGCCTCGCGGGAGCCCGACAGCCCATGCCGGCTCAAGACC
  GTCACCGTATCCACGCTGCCCGCTCTGCGGGAAAGCGACATCGGCTGGAGCGGTGCCCGCACCGGGGCCG
  CGGCCGGGGCTGGGGCCGGGACCGGAGCCGGGGCCGGAGCTGCTGCTGCTGCCGCCTCCGCCGCGTCCCC
  GGGCTCCGCGGGTTCAGCCGGCACAGCCGCGGAGTCTCGTCTCCTTCTCTTTGTGCGTAATGAGCTCCCC
  GGGCGCATCGCGGTACAAGATGACCTGGACAACACTGAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCA
  CAGCAGCGGACATTTCTTTGGTTCACTGGAGGCAGCAGTGGCTGGAGAATGGCACATTGTACTTCCACGT
  TTCCATGAGCAGCTCTGGGCAACTGGCTCAGGCCACTGCTCCCACACTCCAGGAGCCCTCGGAGATCGTT
  GAGGAACAGATGCATATCCTCCACATTTCTGTGATGGGTGGACTCATTGCACTTCTCCTCCTGCTGTTGG
  TGTTCACAGTGGCACTGTATGCCCAGCGACGCTGGCAGAAGCGCCGGCGCATCCCACAGAAGAGCGCAAG
  TACAGAAGCTACTCATGAGATTCACTACATCCCATCAGTGCTGCTGGGTCCTCAGGCCAGGGAAAGCTTC
  CGATCCTCCAGACTTCAGACACACAACTCAGTCATTGGTGTGCCTATCCGGGAAACCCCCATCCTGGATG
  ACTATGACTATGAAGAGGAGGAGGAACCACCCAGGCGAGCCAACCACGTCTCCCGTGAGGATGAGTTTGG
  TAGCCAGATGACCCATGCCCTGGACAGCTTGGGAAGGCCAGGAGAAGAGAAAGTGGAATTTGAGAAGAAA
  GCAGCAGCTGAGGCGACACAGGAAACAGTGGAGTCCCTGATGCAGAAGTTCAAGGAGAGTTTCCGTGCTA
  ACACACCAGTGGAGATCGGTCAGTTGCAGCCAGCCTCGCGCAGCAGCACCTCTGCAGGGAAGAGGAAGCG
  GAGGAACAAATCTCGAGGGGGAATCAGCTTTGGGAGAACCAAGGGGACATCAGGCTCAGAAGCAGATGAC
  GAAACACAGCTGACCTTCTACACAGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTACTGAAAAGCC
  CTGTGAATAAGACAGCCTTAACACTGATTGCTGTGAGCTCCTGCATCTTGGCCATGGTGTGTGGCAACCA
  GATGTCCTGTCCACTTACTGTGAAGGTGACTTTGCATGTGCCTGAACACTTCATTGCAGATGGGAGCAGC
  TTTGTGGTGAGTGAAGGAAGCTACCTGGATATCTCCGATTGGCTAAACCCTGCCAAGCTGTCTCTGTATT
  ACCAGATCAATGCCACCTCGCCATGGGTGAGAGACCTCTGTGGACAGAGGACGACAGATGCCTGTGAACA
  GCTCTGTGACCCAGATACTGGGGAGTGCAGTTGTCATGAAGGTTACGCCCCTGACCCAGTCCACAGACAT
  CTATGTGTTCGCAGTGACTGGGGTCAAAGTGAAGGACCTTGGCCTTACACAACACTGGAGAGGGGCTATG
  ATCTGGTGACAGGAGAGCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAGCTTGGGCCAAGGCCTCTG
  GCTTCCGGTCAGCAAAAGCTTTGTGGTGCCACCGGTGGAGCTGTCCATCAACCCCCTGGCCAGCTGCAAG
  ACGGATGTGCTCGTCACAGAAGACCCTGCGGATGTCAGGGAAGAAGCAATGTTGTCCACGTACTTTGAAA
  CCATCAACGACCTGCTGTCCTCCTTTGGTCCAGTCCGTGACTGCTCAAGGAACAACGGGGGCTGTACCCG
  TAACTTCAAGTGTGTGTCTGACCGCCAGGTGGACTCCTCGGGATGTGTGTGTCCTGAGGAACTGAAGCCC
  ATGAAGGATGGCTCTGGCTGCTATGATCACTCCAAGGGCATCGACTGCTCTGATGGCTTTAATGGAGGGT
  GTGAGCAGCTTTGCCTACAACAAACACTGCCTTTGCCCTATGATACCACCTCCAGCACCATCTTCATGTT
  CTGCGGCTGTGTGGAAGAGTACAAGCTGGCTCCTGATGGGAAATCCTGTCTAATGCTCTCAGATGTCTGT
  GAGGGCCCCAAGTGCCTCAAACCTGACTCCAAATTCAATGACACCCTCTTTGGAGAGATGCTACATGGTT
  ACAACAACCGGACACAACATGTAAACCAAGGCCAAGTCTTCCAAATGACATTCAGGGAGAATAACTTCAT
  CAAGGACTTTCCACAGCTGGCAGATGGGCTGTTGGTGATCCCGCTGCCAGTGGAAGAGCAATGCCGGGGG
  GTTCTCTCTGAGCCCCTTCCAGACCTTCAGCTTCTCACAGGAGACATCAGGTATGATGAGGCCATGGGGT
  ACCCCATGGTGCAACAGTGGCGTGTCCGGAGCAATCTCTATCGTGTGAAGCTGAGTACTATCACCCTCTC
  AGCAGGCTTCACCAATGTCCTAAAGATCCTGACCAAAGAGAGCAGTCGGGATGAGCTGCTGTCCTTCATC
  CAGCACTATGGCTCCCACTACATTGCCGAGGCTTTATACGGTTCTGAGCTCACCTGCATCATCCATTTTC
  CCAGCAAGAAGGTCCAGCAGCAGCTATGGCTCCAATATCAGAAAGAGACCACGGAGCTGGGCAGCAAGAA
  GGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCCGGTCTCCTGACAGCCCAGATGCTATCAGATGAC
  CAGCTCATCTCAGGTGTGGAGATTCGATGTGAGGAGAAGGGCCGCTGTCCATCTACCTGTCACCTTTGCC
  GCCGGCCAGGCAAAGAGCAGCTGAGCCCCACACCAGTGCTGCTGGAGATAAACCGTGTGGTTCCACTTTA
  TACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAATGCACTGATGAGTTCTTACTGGTGTTCT
  GGTAAAGGAGATGTGATTGATGACTGGTGCAGGTGTGACCTCAGTGCCTTTGATGCCAGTGGGCTTCCAA
  ATTGTAGCCCCCTTCCACAGCCGGTGTTACGACTTTCTCCAACAGTGGAGCCTTCCAGCACTGTGGTTTC
  CCTGGAGTGGGTAGATGTTCAGCCAGCCATTGGAACCAAAGTCTCTGACTATATTCTGCAGCACAAGAAA
  GTGGATGAATACACAGACACGGACCTATACACAGGAGAATTCCTGAGTTTTGCCGATGACTTACTTTCTG
  GCCTGGGTACATCTTGTGTAGCAGCTGGTCGAAGCCATGGAGAGGTTCCTGAAGTCAGTATCTACTCGGT
  GATCTTCAAGTGCCTGGAGCCAGATGGTCTTTACAAGTTCACTCTGTATGCTGTGGATACTCGAGGGAGG
  CACTCAGAGCTCAGCACAGTGACTCTAAGGACAGCTTGTCCACTGGTAGATGACAACAAAGCAGAAGAGA
  TAGCGGACAAGATTTACAATCTATACAATGGTTACACAAGTGGCAAGGAGCAGCAGACTGCTTACAACAC
  ACTGATGGAAGTCTCCGCCTCCATGCTCTTCCGTGTCCAGCACCACTACAACTCTCACTATGAAAAGTTT
  GGTGACTTCGTCTGGAGGAGTGAGGATGAGCTAGGACCAAGGAAGGCTCATCTGATTCTGCGGCGGCTGG
  AGCGGGTCAGCAGCCACTGTTCTAGTCTCTTGAGAAGCGCCTACATCCAGAGTCGTGTGGACACCATACC
  CTATCTCTTCTGCCGCAGTGAGGAGGTCCGGCCTGCAGGAATGGTGTGGTACAGCATCCTCAAAGACACC
  AAAATCACATGTGAGGAGAAGATGGTGTCTATGGCCCGAAACACGTACGGGGAAACCAAAGGCCGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207109
• Insert Size: 4059 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207109.2, NP_996992.1
• RefSeq Size: 4831 bp
• RefSeq ORF: 4059 bp
• Locus ID: 56079
• UniProt ID: Q80Z10
• Cytogenetics: 4 C1
• Gene Summary: Mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. Promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1 (PubMed:20573900). Selectively binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes that contain lipids with a phosphoinositide headgroup (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and it encodes the longer isoform (b). Isoform b is most similar to the protein encoded by transcript variant 3 of the human gene. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.