Pard3 (NM_033620) Mouse Untagged Clone

CAT#: MC224270

Pard3 (untagged) - Mouse par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pard3
• Synonyms: AA960621; AI256638; Asip; D8Ertd580e; Par-3; Par3; Pard-3; Pard3a; Phip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224270 representing NM_033620
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGACCGTGTGCTTCGGGAGGACCCGGGTGGTCGTGCCGTGCGGAGATGGCCGCATGAAAGTTT
  TCAGCCTCATCCAGCAGGCGGTGACCCGCTACCGGAAGGCCGTGGCCAAGGATCCAAACTACTGGATACA
  GGTGCATCGCTTGGAGCATGGAGATGGAGGGATTCTAGACCTGGATGACATCCTCTGTGACGTTGCTGAT
  GACAAAGACAGACTGGTAGCAGTATTTGATGAACAGGATCCCCACCATGGAGGAGATGGTACCAGCGCCA
  GCTCCACGGGAACCCAGAGTCCAGAGATATTCGGCAGTGAGCTGGGCACCAACAATGTTTCTGCTTTTCA
  GCCTTATCAAGCCACAAGTGAAATTGAGGTCACGCCTTCAGTTCTTCGGGCAAATATGCCTCTTCATGTC
  CGCCGGAGCAGCGACCCAGCTTTAACTGGCCTTTCCACTTCTGTCAGTGATAACAACTTTTCCTCAGAGG
  AGCCCTCCAGGAAAAACCCCACCCGCTGGTCCACGACAGCTGGCTTTCTCAAGCAGAACACCGCTGGAAG
  TCCCAAAACCTGCGACAGGAAGAAAGATGAAAACTACAGAAGCCTTCCACGGGATCCCAGTAGCTGGTCC
  AACCAGTTCCAGCGAGACAACGCCCGCTCCTCCCTGAGCGCCAGCCACCCAATGGTAGACCGGTGGCTGG
  AGAAGCAAGAACAGGATGAGGAAGGCACAGAAGAAGACAGCAGCCGAGTGGAGCCGGTTGGACATGCTGA
  TACCGGATTGGAGAACATGCCCAACTTTTCCCTCGATGATATGGTAAAGCTCGTACAAGTCCCCAACGAT
  GGAGGGCCCCTGGGAATCCATGTAGTGCCTTTCAGTGCTCGAGGCGGCAGAACATTGGGGTTGTTAGTGA
  AGCGGTTGGAGAAAGGCGGTAAGGCTGAGCAAGAAAACCTTTTCCATGAGAATGACTGCATTGTGAGGAT
  TAACGATGGAGATCTTCGAAACAGAAGATTTGAGCAAGCACAACATATGTTCCGCCAAGCTATGCGTGCG
  CGTGTCATTTGGTTCCATGTGGTCCCTGCAGCAAACAAGGAGCAATATGAACAACTGTCCCAACGCGAGA
  AGAACAACTACTCCCCAGGCCGCTTCAGCCCTGACAGCCACTGTGTGGCCAACAGGAGTGTGGCCAACAA
  TGCCCCTCAAGCATTGCCCAGAGCACCCAGACTGAGTCAGCCACCCGAGCAGCTGGATGCTCACCCCCGA
  CTACCTCATAGTGCTCACGCCTCAACCAAACCACCCGCAGCCCCGGCCTTGGCTCCACCCAGTGTGCTTA
  GTACCAACGTAGGCAGTGTGTACAACACGAAGAAAGTAGGCAAGAGGCTCAACATCCAGCTTAAGAAAGG
  TACAGAAGGACTGGGATTCAGCATCACCTCCCGGGACGTCACCATAGGTGGCTCAGCTCCCATTTATGTC
  AAGAATATCCTTCCTCGAGGGGCTGCCATTCAGGATGGCAGACTCAAGGCAGGAGACCGGCTAATAGAGG
  TCAATGGAGTAGATTTAGCAGGCAAATCCCAGGAGGAAGTTGTTTCCCTGTTGAGAAGCACCAAGATGGA
  GGGGACTGTGAGCCTTCTGGTCTTTCGTCAGGAAGAGGCTTTCCACCCAAGGGAAATGAATGCTGAACCA
  AGCCAGATGCAGACTCCAAAAGAAACGAAAGCTGAAGATGAGGACGTTGTTCTCACACCCGATGGTACCA
  GGGAGTTTCTGACTTTTGAAGTTCCACTGAATGACTCAGGATCTGCAGGGCTTGGTGTCAGTGTCAAGGG
  GAACCGTTCCAAAGAGAACCACGCAGATTTGGGGATCTTCGTTAAATCCATTATCAATGGTGGAGCTGCA
  TCTAAAGATGGAAGGCTGAGGGTAAATGACCAGCTGATAGCTGTGAATGGAGAATCTCTACTGGGCAAAG
  CCAACCAGGAAGCCATGGAGACTCTACGGAGGTCCATGTCCACCGAGGGCAACAAGCGTGGCATGATCCA
  GCTCATTGTGGCGAGGCGGATCAGCAGATGTAACGAGCTGCGGTCTCCTGGGAGCCCTGCTGCACCTGAG
  CTGCCCATCGAGACAGAACTGGATGACCGAGAACGCAGGATCTCACACTCCCTCTACAGTGGGATCGAGG
  GGCTGGATGAGTCGCCCACCAGGAACGCAGCACTGAGCAGGATAATGGGTAAATGCCAGCTGTCCCCCAC
  GGTGAACATGCCTCACGATGACACTGTCATGATTGAAGATGACAGGCTGCCTGTGCTCCCTCCTCACCTC
  TCTGACCAGTCCTCCTCCAGCTCCCATGATGACGTTGGATTCATAATGACAGAAGCAGGCACGTGGGCCA
  AAGCTACCATCAGTGACTCAGCCGACTGCTCATTGAGTCCAGATGTTGATCCGGTTCTCGCTTTTCAACG
  GGAAGGATTTGGACGCCAGAGTATGTCAGAAAAACGCACAAAGCAATTTTCAGATGCCAGTCAATTGGAT
  TTCGTTAAAACACGAAAATCAAAAAGCATGGATTTAGTAGCTGACGAGACTAAACTCAATACAGTGGATG
  ACCAGAGAGCAGGCTCTCCCAGTAGAGATGTGGGACCCTCCTTAGGTCTGAAGAAATCTAGCTCCTTAGA
  AAGTCTGCAGACCGCTGTCGCCGAGGTGACGCTGAACGGGAACATTCCTTTCCACCGCCCTCGGCCACGA
  ATCATCCGGGGAAGGGGCTGCAACGAAAGCTTCAGAGCCGCCATTGACAAGTCCTACGACAAGCCCATGG
  TTGATGATGACGACGAAGGCATGGAGACCTTGGAAGAAGACACAGAGGAAAGCTCGAGGTCAGGGAGGGA
  GTCCGTGTCCACGTCCAGCGATCAGCCTTCCTATTCTCTGGAGAGGCAAATGAATGGAGACCCAGAGAAA
  AGGGACAAGACAGAGAGGAAAAAGGACAAAGCCGGAAAGGATAAGAAGAAAGACCGAGAGAAGGAGAAGG
  ATAAACTGAAAGCCAAGAAGGGGATGCTGAAAGGCTTGGGGGACATGTTCAGGTTTGGCAAACATCGAAA
  AGATGACAAGATGGAAAAAATGGGTCGAATAAAAATCCAGGATTCTTTCACCTCAGAAGAGGACAGGGTG
  CGGATGAAGGAAGAACAGGAGAGGATTCAAGCCAAAACTCGAGAGTTTAGGGAGCGGCAAGCCCGAGAGC
  GTGACTATGCAGAGATCCAAGATTTCCATCGGACGTTTGGCTGTGATGACGAGTTGCTGTATGGGGGCAT
  GTCATCCTATGAAGGCTGCTTGGCTCTCAATGCCAGACCCCAGAGCCCAAGAGAAGGGCACCTGATGGAC
  ACTTTGTATGCCCAAGTAAAGAAACCTCGGAGCTCCAAACCTGGAGACAGCAATCGATCCACTCCTAGCA
  ACCATGACCGGATACAGCGTCTACGGCAAGAGTTCCAGCAAGCCAAACAGGATGAGGATGTGGAAGACCG
  GCGCCGTACCTACAGCTTTGAGCAATCTTGGTCCAGCTCGCGGCCGGCATCGCAGAGTGGTCGGCACTCG
  GTGTCCGTGGAGGTTCAAGTACAACGGCAGCGCCAGGAGGAGCGAGAGAGCTTCCAGCAGGCCCAGCGCC
  AGTACAGCTCACTGCCAAGACAAAGCAGGAAGAATGCCAGCTCCATATCACAGGATTCCTGGGAACAGAA
  CTACGCCCCTGGTGAAGGCTTCCAGAGTGCCAAGGAGAACCCCAGGTATTCCAGTTACCAGGGCTCAAGG
  AACGGCTATCTAGGCGGGCACGGCTTTAATGCCAGGGTCATGCTGGAGACCCAGGAGCTTCTCCGACAGG
  AACAGAGGCGGAAAGAGCAGCAGCTGAAGAAGCAGCCTCCAGCTGATGGAGTCAGAGGGCCCTTCCGGCA
  AGATGTGCCCCCTTCTCCATCTCAGGTGGCTAGGCTGAACAGACTGCAGACACCGGAGAAAGGGCGGCCC
  TTCTACTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033620
• Insert Size: 4002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033620.2, NP_296369.2
• RefSeq Size: 5830 bp
• RefSeq ORF: 4002 bp
• Locus ID: 93742
• Cytogenetics: 8 74.66 cM
• Gene Summary: Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 5. The encoded isoform (3) is shorter than isoform 5.