Cngb1 (NM_001195413) Mouse Untagged Clone

CAT#: MC224253

Cngb1 (untagged) - Mouse cyclic nucleotide gated channel beta 1 (Cngb1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cngb1
• Synonyms: Cngb1b; Garp2; Gm1959
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224253 representing NM_001195413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGGCTGGGTCCAAAGGGTGCTGCCTCAGCCTCCGGGGACCCCTCAGAAGACCGTGGAGACAGCAG
  GACCACAGCCAGAAACAGAGTCGAAACCAGAGGCCAACCCACAACCGGAGCCAGAACCTCAGCAGGAACC
  AGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAA
  CCTGAACCTGAACCAGTACCTGAAGAGGCTCCACCTGAGGTCCAGGCCCTGCCACCAGAGGAGCCAATGG
  AAGGAGAGGGAGAGGCTGAGGCTGGTCCCAGCCTTCAAGAGACCCAGGTAGCTGACCCTGCTCAGCCCAC
  CTCCCAGGCCCAGGTTGCTGTTGCCAAGGTGAACAGGCCCAGCTCCTGGATGTTGAGCTGGTTCTGGAGA
  GGCATGCAGAAGGTCGTGCCACAGCCTGTCTGCAGCAATGGGGGCCAGAACCTGGCTGCCGGAGAGCGAG
  ACCCAGATCAGGGTGGAGCACAGATCCCAGAACCTTGTGGCACTGGGGACCCAGGATCTGCAGAAGCTTC
  CGGGACTCAGGACACTGAGCCCAGCCTTTGGCTGCTCAGGTGGCTTGAGCAGAATCTGGAAAAAGTGCTG
  CCCCAGCCCCCTCCGCCTTCCCTGGCCTGGAAAGTTGAACCTGAGGCTGCTGTCTTGGATCCAGATCCTC
  CAGGAACCCCTATGCAGATGGAGCCCACAGAGAGCCCCTCCCAGCCTAATCCTGGACCCCTGGAGCCTGA
  GGAAGAGCCAGCTGCAGAGCCCCAGCCTGGCTTCCAATCCTCTTCCCTGCCACCACCCGGGGACCCCGTC
  AGGCTGATTGAGTGGCTCCTACACAGACTGGAGATGGCCCTGCCTCAGCCCGTGCTCCATGGGAAGGCTG
  CAGAGCAAGAGCCTGGCTGCCCTGGGATGTGTGACGTACAGACCATCAGCATCCTCCCCGTGGAACAGGT
  GGAACATGATCTTGTCCTGGAGGAGGTGGACTCTTGCTGGGAGGATGCTCAGCAGGAAGATGGCGCCAGC
  CCGCAGGAGACAGAGGTGGCTCCAGCTCATGAAGAAGAGAGTGAGGCCATAGTGGAGATACCCAGGGAGC
  TGACGAAGATTCAAGAAGAAAGAGAAGATGAGCAGGAAGAGGATGAGGAAGAGAAGGAGGAAGAGAAGAA
  GAAGGGGGAGGAAGAGAAGGAGAAGGAGGAGGAAGAGAAAGAGAAAGAGAAGGAGAAGGAGGAGGAGAAG
  GAAGAGGAAGAGAAGGAGGAGGAGGAGGAGGAAGAAAAGGAAGAGGAAGAGGAGGAAAAGGAAGAAGAGG
  AGAAGGAAGAGGAGGAGAAGGAAGAGGAGGAAGAAGAAGAGGAGGAGGAAGAAGAGCCTATTGTCCTGCT
  GGATAGCTGTTTGGTGGTGCAGGCTGATGTGGACGAGTGCCAGCTAGAAAGGACACCGTCAGAGCTAGCA
  TCGATCCAGGAGCTGCCAGAAGAGAAAGAGGAGAAGGAAGAGGAGAAGGAGGAGGAGAAAGAGGAGGAGG
  AGGAAAAGAAGGAGGAAGAAGTGGAGAAGAAGGAGGAAGGGGAGGCCACAAACTCAACAGTACCAGCCAC
  AAAGGAGCACCCGGAGCTCCAGGTAGAAGACACGGATGCTGATAGTGGCCCCCTCATCCCAGAGGAGACA
  CTCCCTCCACCTGAGAGACCACCACCGTCTCCTGTCAAGTCGGACACCCTCACAGTTCCCGGCGCCGCTG
  CAGCAGGCCACAGGAAGAAGCTACCTTCTCAGGATGATGAGGCCGAAGAGCTCAAGGCCCTGTCACCAGC
  TGAGTCCCCAGTGGTTGCCTGGTCGGACCCCACCACCCCACAGGAGGCTGATGGCCAGGACCGAGCAGCC
  TCCACCGCCAGCCAGAACAGTGCCATCATCAATGACCGGCTCCAGGAGCTGGTGAAGATGTTCAAGGAGC
  GGACGGAGAAGGTGAAGGAGAAGCTCATTGACCCTGACGTCACCTCTGATGAGGAGAGCCCCAAGCCCTC
  CCCGGCCAAGAAGGCCCCAGAACCAGACCCAGCCCAGAAGCCAGCGGAGGCAGAGGTGGCAGAGGAGGAA
  CACTACTGCGACATGCTCTGCTGCAAGTTCAAGCGCAGGCCCTTGAAGATGTACCGGTTCCCCCAGAGTA
  TCGACCCACTGACCAACCTCATGTACATCTTGTGGCTGTTCTTTGTGGTGCTGGCCTGGAACTGGAACTG
  CTGGCTGATCCCTGTGCGCTGGGCCTTCCCGTACCAGCGGGCAGACAACATCCACTTCTGGCTCCTCATG
  GATTACTTGTGCGACTTCATCTACCTCCTGGACATCACCGTGTTCCAGATGCGCCTGCAGTTTGTCAAAG
  GCGGGGACATCATTACAGACAAGAAGGAGATGCGTAATAACTACCTGAAGTCTCGCCGGTTTAAGATGGA
  CCTGCTCTGCCTCCTGCCCTTGGACTTTCTCTACTTGAAACTTGGCATCAACCCTCTCCTTCGCCTGCCC
  CGCTGCCTGAAGTACATGGCCTTCTTTGAGTTTAATAACCGCCTGGAAGCCATCCTCAGCAAAGCCTACG
  TTTACAGAGTCATCAGGACCACGGCCTACCTGCTGTACAGCCTGCACCTCAACTCCTGCCTTTACTACTG
  GGCATCAGCCTTCCAGGGCATCGGCTCCACTCACTGGGTTTACGACGGAGTGGGGAACAGTTATATTCGA
  TGCTACTACTGGGCTGTGAAAACCCTCATCACCATCGGAGGACTGCCCGACCCCCAGACGCTCTTTGAGA
  TTGTCTTCCAGCTGCTGAACTATTTTACCGGGGTCTTTGCTTTCTCTGTGATGATTGGACAGATGAGAGA
  TGTGGTGGGGGCCGCCACAGCGGGGCAGACCTACTACCGCAGCTGCATGGACAGCACAGTGAAGTACATG
  AACTTCTACAAGATCCCCAGGTCTGTGCAGAACCGTGTCAAGACCTGGTATGAGTACACCTGGCATTCGC
  AAGGCATGCTGGATGAGTCAGAGCTGATGGTACAACTTCCGGACAAGATGCGACTGGACCTGGCCATTGA
  TGTGAACTACAGTATTGTCAGCAAAGTGGCCCTCTTTCAGGGCTGTGACCGGCAGATGATCTTCGACATG
  CTCAAGCGGCTTCGTTCTGTTGTCTACCTACCCAATGACTATGTGTGCAAGAAGGGGGAGATTGGCCGAG
  AGATGTACATTATCCAGGCGGGGCAGGTGCAGGTGCTGGGCGGCCCAGATGGGAAGGCTGTACTGGTGAC
  ACTCAAAGCCGGATCGGTGTTTGGAGAAATAAGCTTGCTGGCTGTCGGGGGTGGCAACCGGCGCACAGCC
  AACGTGGTGGCCCATGGCTTCACCAACCTCTTCATACTGGACAAGAAGGACTTGAATGAAATTTTAGTGC
  ATTACCCGGAATCTCAGAAGTTACTTCGGAAGAAGGCCAGGCGCATGCTAAGAAATAACAACAAACCCAA
  GGAGGAAAAGAGTGTGCTCATCCTGCCCCCGCGTGCGGGCACCCCGAAGCTCTTCAATGCTGCCCTGGCT
  GCTGCAGGAAAGATGGGCCCCAGGGGAGCCAAGGGCGGCAAGCTCGCCCACCTGAGAGCCAGGCTCAAGG
  AACTGGCTGCACTGGAGGCAGCCGCGCGACAGCAACAGCTGCTGGAACAGGCCAAGAGCTCGCAAGAAGC
  TGGGGGAGAGGAGGGCTCTGGGGCCACAGACCAACCTGCACCCCAGGAGCCACCAGAGCCCAAGGACCCT
  CCGAAGCCACCAGGGCCACCAGAGCCCTCAGCCCAGAGCTCTCCACCTCCAGCCTCAGCAAAGCCCGAGG
  AAAGCACGGGGGAGGCAGCAGGGCCCCCGGAGCCCTCGGTGAGGATCCGTGTGAGTCCAGGCCCTGATCC
  CGGGGAGCAGACACTGTCGGTGGAGGTGCTGGAGGAGAAGAAAGAGGGGGCGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195413
• Insert Size: 3978 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195413.1, NP_001182342.1
• RefSeq Size: 6201 bp
• RefSeq ORF: 3978 bp
• Locus ID: 333329
• Cytogenetics: 8 C5