Tnks (NM_175091) Mouse Untagged Clone

CAT#: MC224230

Tnks (untagged) - Mouse tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase (Tnks), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnks
• Synonyms: 4930554K12Rik; AI662855; ARTD5; C86528; D130072O21Rik; mTNKS1; TANK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224230 representing NM_175091
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCGCGTCGCTCTCAGCATCATCACCACCATCATCAGCAACAGCTCCAGCCCGCCCCAGGGG
  CTTCGGCTCCGCCACCGCCACCTCCCCCGCCGCTCAGCCCGGGCTTGGCCCCGGGACCCACCCCTGCCTC
  GCCTACGGCCGGCGGCCTGGCGCCCTTCGCCTCCCCGCGGCACGGCCTGGCGCTGCCGGAGGGGGATGGC
  AGCCGGGACCCGCCCGACAGGCCGCGATCTCCCGACCCGGTGGACGGCGCCGTCTGCACCGTGGCTGCTC
  CGGCCGCGGTCCCGGCAGCTTCCGCCGCCGTCGGGGTCGCTCCCACCCCAGCCGGCGGCGGCGGCGGCGG
  CGGGAACAACTCCGCGTCGTCCGCTTCTTCCCCGACTTCGTCTTCGTCTTCCTCCCCGTCCTCCCCTGGC
  TCGAGCTTAGCGGAGAGTCCCGAGGCGGCCGGAGTTGGCTCCACGGCAACTTTGGGAGCCGGGGCGGCGG
  GCCTCGGCCCGGGGGTCCCAGCCGTGAGTGGGGCCCTGCGGGAGCTGCTCGAGGCCTGTCGGAATGGGGA
  CGTGTCCCGGGTGAAGAGGTTGGTGGACGCCGCGAACGTGAATGCCAAGGACATGGCCGGCCGAAAGTCT
  TCTCCCCTGCACTTCGCAGCAGGTTTTGGAAGGAAGGATGTTGTAGAACACTTGCTGCAGATGGGTGCTA
  ATGTCCACGCCCGTGATGACGGGGGTCTCATCCCACTTCATAATGCCTGCTCTTTCGGCCATGCTGAGGT
  TGTGAGTCTGTTACTGTGTCAAGGAGCAGATCCGAATGCCAGAGATAACTGGAACTATACACCACTGCAT
  GAAGCTGCCATTAAAGGGAAAATTGATGTCTGTATTGTGCTGCTGCAGCACGGAGCCGATCCAAACATCC
  GGAACACCGATGGGAAATCAGCTCTGGACCTGGCAGATCCTTCAGCAAAAGCTGTCCTCACAGGTGAATA
  CAAAAAAGACGAACTCCTAGAAGCTGCTAGGAGTGGTAATGAAGAAAAACTAATGGCTTTACTGACTCCT
  CTAAATGTGAATTGCCATGCAAGTGATGGACGAAAGTCTACTCCGTTACACCTGGCAGCAGGCTACAACA
  GAGTTCGGATAGTTCAGCTGCTTCTACAGCATGGTGCTGATGTCCACGCGAAAGACAAAGGTGGACTTGT
  ACCTCTTCATAATGCATGCTCATACGGACACTATGAGGTCACAGAACTGCTGCTAAAGCATGGAGCTTGT
  GTCAACGCTATGGATCTGTGGCAGTTCACACCGCTGCATGAAGCTGCTTCAAAGAACCGGGTAGAAGTCT
  GTTCTCTGTTACTTAGTCACGGTGCCGATCCAACCTTGGTCAATTGCCATGGCAAAAGTGCTGTCGACAT
  GGCTCCAACTCCTGAGCTCCGGGAAAGACTGACATATGAATTTAAAGGCCATTCTTTACTCCAAGCCGCC
  AGAGAAGCAGACCTGGCCAAAGTTAAAAAAACGCTTGCTTTGGAAATCATTAACTTCAAGCAGCCACAGT
  CTCATGAAACTGCATTGCACTGTGCTGTGGCCTCCTTGCATCCCAAACGAAAGCAGGTGGCAGAGCTGTT
  ACTGAGGAAGGGAGCCAACGTCAACGAAAAGAACAAGGATTTCATGACCCCTCTGCATGTTGCAGCTGAA
  AGAGCTCATAATGATGTCATGGAAGTTCTGCATAAGCACGGAGCAAAGATGAATGCACTGGATAGTCTTG
  GTCAGACTGCTTTGCACAGAGCTGCCTTAGCGGGCCACCTGCAGACCTGCCGCCTCCTGCTGAGTTATGG
  CTCTGATCCTTCCATCATCTCCCTGCAAGGCTTCACGGCAGCGCAGATGGGGAATGAAGCAGTGCAACAG
  ATTCTGAGCGAGAGTACTCCTATGCGCACTTCGGACGTGGACTATCGTCTCTTAGAGGCATCGAAAGCTG
  GTGACTTGGAAACTGTGAAGCAACTCTGCAGCCCGCAGAATGTGAACTGCAGAGACCTGGAGGGCCGGCA
  CTCTACACCCTTACACTTCGCTGCAGGCTATAACCGTGTGTCTGTCGTTGAGTACCTTCTACATCATGGA
  GCTGATGTCCATGCCAAAGACAAAGGTGGCTTGGTTCCCCTTCACAATGCCTGCTCCTATGGACACTATG
  AGGTCGCCGAGCTCTTGGTGAGGCACGGGGCTTCAGTCAATGTGGCCGACCTGTGGAAATTCACCCCTCT
  CCATGAAGCGGCAGCCAAGGGAAAATACGAAATCTGCAAACTCCTTCTAAAACATGGAGCAGATCCAACT
  AAGAAGAACAGAGATGGAAATACACCCCTAGATCTGGTCAAAGAAGGAGACACGGATATTCAGGACCTGC
  TGAGGGGCGATGCTGCCCTGTTGGATGCTGCCAAGAAGGGCTGCCTAGCCAGAGTGCAGAAGCTTTGTAC
  TCCAGAGAATATCAACTGCCGAGACACCCAGGGCAGAAATTCAACCCCTCTCCACCTGGCAGCAGGCTAC
  AATAACCTGGAAGTAGCTGAGTACCTTCTAGAGCACGGGGCTGATGTCAATGCCCAGGACAAAGGAGGGC
  TAATCCCACTTCACAACGCTGCGTCTTATGGGCATGTTGACATAGCAGCGTTACTGATAAAATACAACAC
  GTGTGTAAACGCAACGGATAAGTGGGCCTTTACTCCCCTTCACGAAGCTGCCCAGAAAGGAAGGACGCAG
  TTATGTGCCCTGCTCCTAGCACATGGCGCAGACCCAACCATGAAGAACCAGGAAGGTCAGACACCTTTAG
  ATCTGGCGACGGCTGATGACATCCGGGCTCTGCTGATAGATGCCATGCCCCCTGAGGCCTTACCTACCTG
  TTTTAAACCTCAGGCAACTGTAGTTAGTGCCTCTCTGATCTCACCAGCGTCCACCCCCTCCTGCCTCTCT
  GCTGCCAGCAGCATAGATAACCTCACTGGCCCGTTAACGGATCTGGCTGTAGGAGGGGCCTCCAATGCAG
  GGGATGGCGCTGCGGGTGCAGAAAGGAAGGAAGGAGAAGTTGCGGGTCTTGACATGAACATCAGCCAGTT
  CCTTAAGAGCCTTGGCCTTGAGCACCTGAGGGACATTTTTGAAACAGAGCAGATTACGCTAGATGTGTTG
  GCTGATATGGGTCATGAAGAATTGAAAGAAATAGGCATCAATGCATATGGACACCGCCACAAGTTGATCA
  AGGGTGTTGAAAGACTTTTAGGTGGACAACAAGGAACCAATCCATATTTGACTTTTCACTGTGTGAATCA
  GGGAACTATTTTGCTAGATCTTGCTCCAGAAGATAAAGAATATCAATCAGTAGAAGAAGAGATGCAGAGC
  ACTATTCGAGAGCACAGAGACGGTGGGAACGCTGGCGGCATCTTCAACAGATACAACGTCATTCGAATTC
  AGAAAGTTGTGAATAAGAAGCTGAGGGAGCGATTCTGCCACAGACAAAAGGAGGTGTCTGAGGAGAACCA
  CAACCATCACAATGAGCGCATGCTGTTCCACGGTTCTCCTTTCATTAATGCAATTATTCATAAAGGGTTT
  GATGAGCGACATGCGTACATTGGAGGGATGTTTGGAGCTGGAATTTACTTTGCTGAAAACTCCTCAAAAA
  GCAATCAGTATGTTTATGGAATTGGAGGAGGAACAGGCTGTCCCACACACAAAGACAGATCATGTTATAT
  ATGTCACAGACAAATGCTTTTCTGTAGAGTGACCCTTGGAAAGTCCTTCCTGCAATTCAGCACCATGAAA
  ATGGCCCATGCACCCCCAGGCCATCACTCAGTCATCGGTAGACCAAGTGTCAATGGGCTGGCATATGCTG
  AATATGTCATCTACAGAGGGGAACAGGCATATCCAGAATATCTCATCACCTACCAGATCATGAAGCCAGA
  AGCCCCTTCACAGACCGCAACGGCAGCAGAGCAGAAGACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175091
• Insert Size: 3963 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175091.3, NP_780300.2
• RefSeq Size: 6444 bp
• RefSeq ORF: 3963 bp
• Locus ID: 21951
• UniProt ID: Q6PFX9
• Cytogenetics: 8 21.16 cM
• Gene Summary: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity.[UniProtKB/Swiss-Prot Function]