Sos1 (NM_009231) Mouse Untagged Clone

CAT#: MC224217

Sos1 (untagged) - Mouse son of sevenless homolog 1 (Drosophila) (Sos1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sos1
• Synonyms: 4430401P03Rik; 9630010N06; AI449023
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224217 representing NM_009231
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGCAGCAGCTGCCTTACGAGTTTTTCAGCGAGGAGAACGCGCCCAAGTGGCGGGGGCTGCTGG
  TGCCTGCGCTGAAAAAGGTTCAGGGGCAAGTTCACCCTACTCTTGAGTCTAATGATGATGCTCTTCAGTA
  TGTTGAAGAATTAATTTTGCAATTACTAAATATGCTATGCCAAGCTCAGCCCCGGAGTGCTTCAGATGTG
  GAGGAACGTGTTCAAAAGAGTTTTCCTCATCCAATTGATAAGTGGGCAATAGCTGATGCCCAATCAGCCA
  TTGAAAAGAGGAAGAGACGAAATCCTTTATCGCTGCCAGCAGAAAGAATTCATCATTTATTAAGGGAGGT
  CCTCGGTTATAAAATTGACCACCAGGTTTCTGTTTACATAGTAGCAGTATTAGAATACATTTCTGCAGAT
  ATTTTAAAGCTCGTGGGGAATTATGTAAGAAATATACGGCATTATGAAATTACAAAACAAGACATTAAAG
  TGGCAATGTGTGCTGATAAGGTATTGATGGATATGTTTCATCAAGATGTAGAAGATATAAATATCTTATC
  TTTAACTGATGAAGAGCCTTCCACCTCAGGAGAACAAACTTATTATGATTTGGTAAAAGCATTCATGGCA
  GAAATTCGACAGTATATAAGAGAATTAAATCTAATTATAAAAGTTTTTCGAGAGCCCTTTGTCTCTAATT
  CCAAATTGTTTTCATCTAATGATGTAGAAAACATATTCAGTCGTATAGTAGATATACATGAACTTAGTGT
  AAAGTTACTGGGCCATATAGAAGATACTGTAGAAATGACAGATGAAGGCAGTCCCCACCCATTAGTAGGA
  AGCTGTTTTGAAGACTTAGCAGAAGAACTGGCATTTGACCCGTATGAGTCATATGCTCGGGATATTTTAC
  GACCCGGATTCCATGGCCATTTTCTTAGTCAGTTATCAAAGCCTGGGGCAGCACTTTATTTGCAGTCCAT
  AGGCGAAGGCTTCAAAGAAGCTGTCCAGTACGTCCTGCCCCGGCTGCTGCTTGCCCCTGTGTACCACTGT
  CTGCATTACTTTGAACTTCTGAAGCAGTTAGAAGAAAAGAGTGAAGATCAAGAAGACAAGGAGTGTATGA
  AGCAAGCAATAACAGCCCTGCTTAATGTCCAAAGTGGCATGGAAAAAATTTGCTCCAAAAGTCTTGCAAA
  ACGAAGACTAAGTGAGTCTGCATGTCGGTTTTACAGCCAGCAGATGAAGGGGAAACAGCTAGCCATCAAG
  AAGATGAACGAGATCCAGAAGAACATTGATGGCTGGGAGGGGAAGGACATTGGACAGTGTTGCAATGAGT
  TCATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACACGAGAGACACATATTTCTCTTCGATGGCTT
  AATGATTTGCTGTAAATCAAACCATGGGCAGCCAAGACTCCCTGGTGCTAGCAGTGCAGAATACCGGCTT
  AAAGAAAAGTTTTTTATGCGAAAGGTACAGATTAATGATAAAGATGACACCAGTGAGTACAAGCATGCTT
  TTGAAATCATTCTGAAAGATGGCAATAGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAAAACAACTG
  GATGGCAGCACTGATCTCTTTGCAGTACCGCAGCACCCTGGAGAGGATGCTGGACGTAACGGTGCTGCAG
  GAGGAGAAGGAGGAGCAGATGAGGCTGCCCAGTGCTGAAGTGTACAGGTTTGCAGAACCTGACTCCGAGG
  AGAATATTCTATTCGAAGAGAATGTGCAGCCCAAAGCTGGGATCCCCATTATCAAGGCAGGGACAGTGCT
  TAAGCTCATTGAGAGGCTTACCTACCACATGTACGCAGATCCAAATTTTGTTCGGACGTTTCTTACAACA
  TACAGGTCCTTTTGCAGACCTCAAGAACTACTGAGTCTTCTGATAGAAAGATTTGAAATTCCAGAGCCTG
  AGCCAACAGAAGCTGATCGCATAGCTATAGAGAATGGAGATCAGCCCCTGAGTGCAGAGCTGAAGAGGTT
  TAGAAAGGAATATATTCAGCCTGTGCAGTTGAGGGTGTTAAATGTGTGTCGGCACTGGGTGGAGCACCAT
  TTCTATGACTTTGAAAGAGATGCAGACCTTTTACAGAGAATGGAGGAATTTATTGGAACAGTAAGAGGTA
  AAGCAATGAAAAAATGGGTCGAATCCATCACTAAGATAATCCAAAGGAAAAAAATTGCAAGAGACAATGG
  CCCAGGTCATAACATTACATTTCAGAGCTCACCTCCCACAGTTGAGTGGCACATAAGCAGACCTGGGCAC
  ATAGAGACTTTTGACTTGCTCACCTTACACCCAATAGAAATTGCTCGGCAACTCACTTTACTTGAATCAG
  ATCTATACCGGGCTGTGCAGCCATCAGAATTAGTTGGAAGTGTGTGGACAAAAGAAGATAAAGAAATTAA
  TTCTCCCAACCTTCTGAAGATGATTCGGCACACCACTAACCTCACTTTGTGGTTTGAGAAATGTATTGTA
  GAAACAGAAAACTTAGAAGAAAGAGTAGCTGTAGTAAGTCGGATAATTGAGATTCTACAAGTCTTTCAAG
  AGCTGAACAACTTCAATGGTGTCCTGGAAGTTGTCAGTGCTATGAACTCGTCACCTGTTTACAGACTAGA
  CCACACATTTGAGCAAATACCAAGCAGACAAAAGAAAATTTTAGAAGAAGCTCATGAATTGAGTGAAGAT
  CACTATAAGAAATATTTGGCAAAACTCAGGTCTATTAATCCACCGTGTGTGCCTTTCTTTGGAATTTATC
  TCACAAATATCCTGAAGACAGAAGAGGGCAACCCTGAGGTCCTGAGGAGACACGGGAAAGAGCTTATTAA
  CTTCAGCAAGAGGAGGAGAGTGGCCGAGATCACAGGCGAGATCCAGCAGTACCAGAACCAGCCCTACTGC
  TTACGGGTGGAGCCGGACATCAAGAGGTTCTTTGAAAACTTGAATCCAATGGGAAACAGCATGGAGAAAG
  AATTTACAGACTATCTGTTCAACAAATCCCTAGAAATAGAACCCCGGCACCCTAAGCCTCTTCCGAGATT
  CCCAAAAAAATACAGCTATCCCCTAAAATCTCCTGGTGTTCGTCCATCAAATCCAAGACCAGGAACCATG
  AGACATCCCACACCTCTGCAGCAGGAGCCAAGAAAAATTAGCTACAGTCGGATTCCTGAAAGTGAGACGG
  AAAGCACAGCATCTGCACCAAACTCCCCTCGGACCCCACTGACGCCGCCCCCTGCATCTGGCACCTCCAG
  CAACACAGATGTTTGCAGCGTGTTCGATTCTGACCACTCGGCAAGCCCTTTTCATTCAAGATCTGCTTCA
  GTCTCATCTATAAGTTTATCCAAGGGCACTGATGAAGTGCCTGTCCCCCCTCCTGTACCCCCTCGAAGAC
  GTCCAGAGTCTGCCCCAGCTGAATCCTCCCCATCCAAGATTATGTCTAAGCACTTGGACAGCCCCCCAGC
  TATTCCTCCTAGGCAACCCACATCCAAAGCCTATTCACCACGCTATTCAATATCAGATCGGACCTCTATA
  TCAGATCCTCCTGAAAGCCCTCCCTTGTTACCACCACGGGAACCTGTGAGGACACCTGATGTTTTCTCAA
  GCTCACCATTACATCTCCAACCTCCTCCTTTGGGCAAAAAGAGTGATCATGGCAACGCCTTCTTCCCAAA
  CAGCCCATCCCCTTTTACACCGCCACCCCCCCAAACCCCCTCTCCTCATGGCACGAGAAGGCATCTGCCA
  TCACCACCACTGACACAGGAGATGGACCTCCATTCCATTGCTGGGCCTCCTGTTCCTCCACGACAAAGCA
  CTTCTCAACTTATCCCCAAACTCCCTCCAAAAACTTACAAAAGGGAGCACACACACCCATCCATGCATAG
  AGATGGACCACCACTGCTGGAGAATGCCCATTCTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009231
• Insert Size: 3960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009231.2, NP_033257.2
• RefSeq Size: 8919 bp
• RefSeq ORF: 3960 bp
• Locus ID: 20662
• UniProt ID: Q62245
• Cytogenetics: 17 50.67 cM
• Gene Summary: Promotes the exchange of Ras-bound GDP by GTP. Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (By similarity). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (PubMed:10499589, PubMed:11524436).[UniProtKB/Swiss-Prot Function]