Oplah (NM_153122) Mouse Untagged Clone

CAT#: MC224159

Oplah (untagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Oplah
• Synonyms: 1700010G02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224159 representing NM_153122
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCCCAGAAGAGCGCTTCCATTTTGCCATCGACCGTGGTGGCACCTTTACAGATGTCTTTGCCC
  AGTGCCCAGGAGGGCATGTACGTGTCCTGAAGCTGCTCTCAGAGGATCCTGCCAACTATGCAGACGCACC
  CACAGAGGGCATCCGCCGAATTCTAGAGCAGGAGAGGGGTGTGCTGCTGCCCCGAGGCCGGCCGCTAGAC
  ACCAGTCACATCGCCAGCATCCGCATGGGTACCACCGTGGCCACCAATGCTCTGTTGGAACGGCAGGGAG
  AACGGGTGGCACTGCTGGTGACTCGCGGTTTCCGAGACCTGCTGCATATTGGCACTCAGGCCCGCCCAGA
  CCTCTTTGACCTGGCTGTGCCCATGCCAGAGGTACTGTATGAGGAGGTCGTGGAGGTGGACGAGCGAGTG
  CTGCTGTATCGTGGAGAACCCGGTGCCGGCTCTCCCGTCAAAGGCTGTACAGGGGACCTGCTAGAGATAC
  AGCAGCCTGTGGATCTGGCAGCCCTGCGTGGGAAGCTGGAGGGGCTCTTGACTCGGGGCATTCACAGCCT
  GGCAGTGGTACTCATGCATTCGTACACGTGGGCCCAGCACGAGCAGCAGGTGGGCACACTGGCCCGGGAG
  CTGGGCTTCACGCACGTCTCCTTGTCCTCGGAAGTCATGCCCATGGTGCGGATTGTTCCTCGGGGGCATA
  CGGCCTGTGCAGATGCTTACCTCACTCCCACCATCCAGCGCTATGTGCAGGGCTTCCGCCGAGGCTTCCA
  GGGCCAGCTAAAGAATGTGCAAGTCCTCTTCATGCGCTCCGATGGTGGCCTGGCACCCATGGATGCCTTC
  AGTGGCTCCCGGGCTGTGCTCTCTGGCCCTGCTGGTGGTGTGGTTGGCTACTCCACTACCACCTACCAGC
  TGGAAGGTGGTCAGCCTGTCATTGGCTTTGACATGGGAGGCACATCCACAGATGTGAGCCGCTATGCTGG
  AGAGTTTGAGCACGTCTTTGAGGCTAGCACGGCAGGTGTTACCCTCCAGGCCCCACAGCTGGACATCAAT
  ACGGTGGCAGCTGGCGGGGGTTCCCGCCTCTTCTTCAGGTCTGGCCTCTTTGTGGTTGGTCCAGAGTCAG
  CAGGTGCCCACCCAGGTCCTGCCTGCTACCGCAAAGGGGGTCCTGTGACAGTGACAGATGCTAATCTGGT
  TCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCAGGAGAAGACCAGCCATTGTCTCCC
  GAGGCTTCCCGAAAGGCTCTAGAGGCTGTGGCCATGGAGGTCAACAGTTTCTTGGCCAGTGGACCCTGCC
  CAGCTTCCCAGCTAAGTCTGGAGGAGGTGGCCATGGGGTTTGTGCGTGTTGCCAACGAAGCCATGTGCCG
  GCCTATCCGTGCCCTCACACAGGCACGAGGCCACGACCCCTCAGCCCATGTACTGGCTTGCTTTGGAGGA
  GCCGGTGGGCAACACGCTTGTGCCATTGCCCGGGCCCTGGGTATGGACACTGTGCATATTCACAGGCACA
  GCGGGCTGCTGTCAGCACTGGGACTGGCCTTGGCCGATGTGGTTCACGAAGCACAGGAGCCCTGTTCCCT
  GTCTTACACACCTGAAACCTTTGCACAACTGGACCAGAGACTGAGCCGCCTGGAGGAGCAGTGTGTGGAT
  GCCCTGCAGGCCCAGGGCTTCTCTAGGTCTCAGATCAGCACCGAGAGCTTCCTGCACCTTCGCTACCAAG
  GCACTGACTGTGCCCTAATGGTGTCTGCCAATCAGCATCCGGCCACAACCTGCTCACCCCGTGCTGGTGA
  CTTTGGAGCAGCGTTTGTCGAGAGGTACATGAGAGAGTTTGGCTTCATCATTCCTGAGCGCTCGGTGGTG
  GTAGATGATGTGCGTGTGAGGGGAACTGGCCGTAGTGGACTTCAGCTGGAGGAGACCTCCAAAATCCAGA
  GTGGACCTCCGCATGTGGAAAAGGTGACCCAGTGCTACTTTGAAGGGGGTTATCAGGAGACTCCCGTGTA
  CCTTTTAGGAGAACTAGGCTACGGGCATCAGCTTCAAGGGCCCTGCCTGATCATTGACAACAACAGCACC
  ATCCTTGTAGAACCAGGTTGCCAAGCGGAGGTGATTGAGACAGGGGACATCCGCATTTCTGTGGGAGCCG
  AGGCTCCCAGTATGATAGACACCAAGCTTGACCCCATCCAGTTGTCTATTTTCTCACACCGTTTCATGAG
  CATTGCTGAGCAGATGGGCCGCATCCTACAGCGCACAGCCATCTCTACCAACATCAAGGAACGCCTCGAC
  TTCTCCTGTGCCCTCTTTGGGCCAGATGGGGGCCTCGTCTCCAATGCTCCCCACATTCCTGTGCACCTGG
  GTGCCATGCAAGAGACTGTACAGTTCCAGATTCAGCACTTAGGAGCCGACCTCCATCCTGGTGATGTGTT
  GCTTAGTAACCATCCCAGTGCAGGGGGCAGCCATCTTCCTGACCTGACTGTCATCACACCGGTGTTTTGG
  CCAGGTCAGTCGAGGCCTGTGTTCTACGTGGCTAGCCGAGGGCACCACGCGGACATTGGAGGTATCACCC
  CGGGTTCTATGCCTCCTCACTCTACCACACTGCAGCAGGAGGGTGCCGTTTTTCTGTCCTTCAAACTGGT
  CCAGGGAGGAGTCTTCCAGGAAGAGGCGGTGACAGAGGCCTTACAGGCCCCAGGCAAGATCTCTGGCTGC
  AGTGGGACCAGGAACCTGCATGACAACCTGTCGGATCTCCGTGCCCAGGTGGCAGCTAACCAGAAAGGCA
  TCCAGCTGGTGGGAGAGCTGATCGGACAGTATGGCTTAGATGTGGTGCAGGCCTATATGGGCCATATTCA
  GGCAAATGCTGAACTAGCAGTGCGAGACATGCTTCGTGCTTTTGGAACTTCCCGCCAGGCCAGGGGCCTG
  CCCCTGGAGGTGTCTGCCAAGGATCACATGGACGATGGCTCTCCCATCTGCCTGCATGTTCAGATCAACC
  TGAATCAGGGCAGCGCTGTATTTGACTTCAGTGGTTCTGGGTCTGAGGTGTTTGGCAATCTCAATGCCCC
  TCGAGCCATAACACTGTCTGCTCTCATCTATTGCTTGCGCTGTCTAGTGGGCCGTGACATCCCACTTAAC
  CAGGGTTGCCTGGCTCCTGTGCAAGTCATAATTCCCAAAGGCTCCATATTGGATCCATCCCCAGAGGCAG
  CAGTGGTCGGAGGCAACGTGCTCACATCTCAGCGAGTCGTGGATGTCATCCTGGGGGCTTTTGGGGCCTG
  TGCAGCCTCCCAGGGCTGCATGAACAATGTGACCCTGGGCAATGCCCGTATGGGCTACTATGAGACAGTA
  GCTGGTGGTGCGGGTGCAGGCCCTGGCTGGCATGGGCGCAGTGGTGTACACAGTCACATGACCAACACGC
  GCATTACGGACCCAGAGATTTTGGAGAGCCGGTATCCAGTTATCCTGCGCCGCTTTGAGCTGAGGCCAGG
  CTCTGGAGGCCGAGGCCGCTTCCGGGGAGGTGATGGCGTAGTCCGAGAGCTGGTCTTTCGGGAAGAGGCG
  CTACTGTCTGTCCTCACGGAGCGCCGCGCCTTCCAGCCTTATGGCCTCCACGGGGGAGAGCCTGGTACAC
  GTGGCTTAAACCTCCTGATCAGAAAAGATGGGCGCACAGTGAATCTGGGCGGCAAGACATCCGTGACTGT
  GTATCCCGGGGACGCGTTCTGCCTCCACACGCCTGGGGGTGGGGGCTATGGAGACCCGGAGGACCCAGCA
  CCACCACCAGGCTCGCCCCCGCTATATCCAGCCTTCCCAGAGCGAGGCAGTGTGTATGAGTACCGCCGCG
  CCCAGGAAGCAGTATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_153122
• Insert Size: 3867 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153122.2, NP_694762.1
• RefSeq Size: 3970 bp
• RefSeq ORF: 3867 bp
• Locus ID: 75475
• UniProt ID: Q8K010
• Cytogenetics: 15 D3
• Gene Summary: Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.[UniProtKB/Swiss-Prot Function]