Auts2 (NM_177047) Mouse Untagged Clone

CAT#: MC224100

Auts2 (untagged) - Mouse autism susceptibility candidate 2 (Auts2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Auts2
• Synonyms: 2700063G02Rik; A730011F23Rik; D830032G16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224100 representing NM_177047
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCCCGACGCGGGGACATGGACTCCGGAAAAAGCGGAGGTCTCGATCACAGCGAGACCGGGAGA
  GACGCTCCCGGGCCGGGTTAGGAACCGGTGCGGCGGGTGGTATAGGAGCCGGCCGGACCCGGGCTCCCTC
  GCTGGCTTCATCGTCGGGCTCCGACAAGGAAGACAATGGAAAGCCCCCATCCTCCGCCCCGTCCCGGCCC
  AGACCCCCGCGGAGGAAGCGCAGAGAGTCCACCTCAGCCGAAGAGGACATCATTGATGGATTTGCCATGA
  CCAGCTTTGTTACTTTTGAAGCGCTGGAGAAAGATGTAGCAGTTAAGCCGCAGGAACGTGCGGAGAAACG
  ACAGACTCCCCTGACCAAGAAAAAACGAGAAGCTCTTACCAATGGTTTGTCTTTTCACTCAAAGAAGAGC
  CGGCTCAGCCACTCACACCACTACAGCTCAGATCGAGAGAATGACCGCAATCTCTGCCAGCACCTTGGGA
  AGAGAAAGAAGATGCCTAAGGGACTCAGACAGCTCAAGCCAGGACAGAACAGCTGCAGGGACAGTGACAG
  CGAAAGCGCTAGCGGAGAATCCAAGGGCTTCCAGCGGAGCAGCTCTCGGGAGAGGCTCAGTGATAGTTCA
  GCTCCTTCCAGCTTGGGGACAGGCTACTTCTGCGACAGTGACAGCGACCAGGAAGAGAAGGCTTCAGATG
  CTAGCTCTGAAAAACTCTTCAACACTGTTCTTGTAAACAAAGATCCAGAGTTAGGTGTTGGCGCACTACC
  TGAACACAACCAAGATGCGGGACCAATTGTCCCCAAGATCTCGGGTCTAGAGAGAAGCCAGGAGAAGAGC
  CAGGACTGTTGCAAAGAGCCCGTCTTCGAACCCGTGGTGCTCAAAGACCCACATCCTCAGCTCCCTCAGC
  TACCATCCCAGGCCCAGGCCGAACCTCAGCTTCAGATTCCATCTCCAGGCCCAGACTTGGTACCGCGCAC
  TGAAGCTCCGCCCCAGTTTCCTCCTCCAAGTACACAGCCAGCCCAAGGACCCCCAGAAGCCCAGCTGCAG
  CCTGCCCCGCTGCCTCAGGTGCAGCAGCGGCCACCCCGGCCCCAGTCCCCCAGCCACCTGCTCCAGCAGA
  CTCTTCCGCCTGTGCAGTCACATCCCTCCTCCCAGAGCCTCTCACAGCCACTGTCGGCCTACAACAGCAG
  TAGCCTGAGCCTCAACAGCTTAAGCAGCAGAAGCAGTACCCCGGCAAAAACCCAGCCCGCCCCTCCTCAC
  ATCTCCCACCACCCGTCCGCCTCTCCCTTCCCCCTCTCCCTGCCCAACCACAGCCCTCTGCACAGCTTCA
  CACCCACCCTCCAGCCCCCTGCCCACTCACATCACCCCAATATGTTCGCCCCTCCCACTGCTCTGCCTCC
  TCCCCCGCCGCTGACGTCTGGAAGTCTGCAGGTGCCAGGGCATCCCGCAGGGAGCACGTACTCAGAACAA
  GATATCCTGAGGCAGGAACTGAACACGCGTTTCCTGGCCTCTCAGAGTGCCGACCGAGGGGCCTCCCTGG
  GCCCTCCACCCTACCTGCGGACCGAGTTCCATCAGCACCAGCATCAGCACCAGCACACCCATCAGCACAC
  GCACCAGCATACCTTCACACCGTTCCCCCACGCCATCCCGCCCACTGCCATCATGCCGACGCCAGCACCT
  CCCATGTTTGACAAATACCCCACCAAAGTTGACCCATTCTACCGGCATAGTCTCTTCCACTCCTATCCTC
  CTGCGGTCTCGGGTATACCCCCCATGATCCCACCCACGGGCCCTTTCGGGTCACTCCAAGGAGCATTCCA
  GCCGAAGACATCCAACCCTATCGACGTGGCTGCCCGGCCTGGAACAGTCCCACATACTCTGCTCCAGAAG
  GACCCAAGGTTGACAGATCCTTTCAGACCTATGCTAAGGAAACCAGGAAAGTGGTGCGCCATGCATGTGC
  ATATCGCCTGGCAGATTTATCACCATCAACAAAAAGTCAAGAAACAGATGCAGTCAGACCCACACAAGCT
  GGACTTTGGGCTGAAGCCTGAGTTCCTGAGCCGTCCTCCAGGCCCTAGTCTCTTCGGAGCCATCCATCAC
  CCCCACGATCTGGCACGACCTTCCACTTTATTCTCTGCCGCTGGTGCTGCACATCCTACTGGGACCCCTT
  TTGGGCCACCACCTCATCACAGTAACTTTCTCAACCCTGCCGCTCACCTAGAGCCTTTTAACCGGCCGTC
  TACGTTCACGGGCCTCGCTGCAGTTGGTGGCAATGCCTTCGGGGGACTTGGAAACCCTTCAGTTACACCC
  AACTCAGTGTTCGGCCACAAGGATAGCCCCAGTGTGCAGAACTTTAGCAATCCTCACGAACCCTGGAACC
  GGCTGCACCGAACGCCTCCGTCGTTCCCGACCCCTCCGCCCTGGCTGAAGCCAGGGGAATTGGAGCGCAG
  TGCGTCCGCTGCAGCCCATGACAGAGATAGAGATGTAGATAAACGAGACTCCTCCGTTAGTAAAGATGAC
  AAGGAAAGGGAAAGCGTTGAGAAAAGACACCCCAGCCATCCTTCCCCAGCACCTCCCGTCCCGGTGAGCG
  CCCTGGGCCATAACCGCAGCTCCACCGATCCGACGACCAGGGGTCATTTGAATACTGAGGCTCGTGAGAA
  AGATAAACCAAAAGAGAAGGAGAGAGACCACTCGGGATCCCGAAAGGACCTGACCACCGAAGAGCACAAA
  GCCAAGGAAAGCCATCTGCCCGAGAGAGATGGGCACAGCCACGAGGGACGTGCAGCAGGCGAGGAACCCA
  AGCAGCTGTCCCGGGTGCCATCGCCCTACGTGAGGACACCAGGCGTGGATAGCACCAGGCCTAACAGCAC
  CTCTAGTCGGGAAGCCGAGCCACGCAAGGGTGAGCCGGCCTACGAGAACCCCAAGAAGAACGCCGAGGTC
  AAGGTCAAAGAGGAACGCAAGGAAGATCACGACCTACCTACTGAGGCCCCCCAGGCCCACAGGACCTCAG
  AGGCACCTCCGCCCAGCTCCTCAGCCAGTGCCAGCGTGCACCCAGGGCCTCTGGCATCCATGCCCATGAC
  CGTGGGAGTGACTGGCATCCATGCCATGAACAGTATCGGTAGTCTGGACAGGACACGTATGGTGACCCCA
  TTTATGGGCCTCAGTCCGATCCCAGGAGGAGAGCGTTTCCCATACCCCTCTTTCCACTGGGACCCTATGC
  GGGACCCCTTGAGGGATCCTTACCGTGACTTGGACATGCACCGAAGAGACCCTCTGGGCAGGGACTTTCT
  GCTCAGGAATGACCCCCTGCACCGGCTCTCCACACCCCGGTTGTATGAAGCCGACCGATCGTTCCGGGAC
  AGGGAGCCTCATGATTACAGCCACCACCACCACCACCACCATCACCCGCTGGCCGTGGACCCTCGACGGG
  AGCACGAACGAGGAGGACACCTGGATGAACGCGAGCGGCTGCACGTGCTTCGGGAGGACTATGAGCACCC
  GCGGTTGCACCCTGTGCACCCCGCTTCTCTAGATGGACACCTCCCACACCCGAGCCTGCTCACACCAGGC
  CTGCCTAGTATGCACTACCCTCGCATCAGCCCTACTGCGGGCCACCAGAATGGACTGCTTAACAAGACCC
  CGCCCACTGCGGCGCTTAGCGCCCCACCTCCGCTCATCTCCACATTGGGGGGCCGCCCGGGATCCCCCCG
  GAGGACTACGCCTCTGTCGGCTGAAATCCGAGAGAGGCCACCGTCCCACACACTGAAGGACATTGAAGCT
  CGGTAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_177047
• Insert Size: 3786 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177047.3, NP_796021.2
• RefSeq Size: 6060 bp
• RefSeq ORF: 3786 bp
• Locus ID: 319974
• UniProt ID: A0A087WPF7
• Cytogenetics: 5 G2
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B. As a consequence, the complex mediates transcriptional activation (By similarity). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (PubMed:25533347).[UniProtKB/Swiss-Prot Function]