Nphs1 (NM_019459) Mouse Untagged Clone

CAT#: MC224090

Nphs1 (untagged) - Mouse nephrosis 1 homolog, nephrin (human) (Nphs1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nphs1
• Synonyms: nephrin; NephrinB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224090 representing NM_019459
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTAAGGAAGCCACAGTCAGAGGCCCCGGGGCAAGCCCAGTGCACAGAACTTGCCACCTGATTC
  CCCTGCTGCTCGCGGGAATGCTGACCACAGGCCTGGCCCAGTCGCCAGTCCCCACCTCAGCACCTCGAGG
  CTTCTGGGCTCTATCTGAAAACCTGACTGTGGTGGAAGGGTCGACAGTTAAGCTGTGGTGTGGCGTCAGG
  GCCCCCGGCAGTGTGGTGCAGTGGGCTAAGGATGGGCTGCTTCTGGGTCCAAACCCTAAGATTCCAGGCT
  TCCCAAGGTACAGCCTGGAAGGAGACAGTGCTAAAGGTGAGTTCCACCTGCTTATTGAAGCCTGTGACCT
  CAGCGATGATGCGGAGTACGAGTGCCAAGTCGGCCGCTCCGAGTTGGGTCCCGAGCTCGTGTCTCCCAGC
  GTAATCCTCTCCATCCTAGTTTCCCCCAAGGTGCTTCAGTTAACCCCTGAGGCAGGAAGCACAGTTACCT
  GGGTAGCTGGACAGGAATATGTGGTCACCTGTGTGTCTGGGGATGCAAAGCCAGCACCTGACATCATCTT
  CATCCAGGGTGGACGCACGGTAGAGGATGTCTCCTCCAGCGTGAATGAGGGATCAGAGGAGAAACTCTTC
  TTCACGGAAGCCGAAGCCAGGGTGACACCCCAGAGCTCAGATAATGGGCAGCTGCTAGTCTGCGAGGGGT
  CCAACCCAGCCTTGGCCACTCCCATAAAGGCTTCGTTCACCATGAATATCCTGTTCCCCCCAGGACCTCC
  TGTCATTGATTGGCCAGGCCTGAATGAGGGGCATGTACGGGCAGGGGAGAACCTGGAGCTACCCTGCATA
  GCCAGAGGTGGAAATCCACCTGCGACCCTGCAGTGGCTGAAGAATGGTAAACCAGTGTCCATAGCTTGGG
  GCACAGAGCATGCCCAGGCAGTGGCTCACAGTGTGCTGGTGATGACCGTTCGACCTGAAGACCACGGAGC
  TCGGCTCAGCTGTCAGTCCTACAACAGTGTGTCTGCAGAGACCCAGGAGCGAAGCATCACTCTGCAGGTC
  ACCTTTCCCCCCAGTGCCGTTACCATCCTGGGATCTACATCACAGTCTGAGAACAAAAATGTGACCCTTT
  GCTGCCTTACCAAGTCCAGTCGCCCACGGGTCCTGCTGCGATGGTGGTTGGGTGGACGGCAGTTGCTGCC
  CACGGATGAGACAGTCATGGATGGCCTGCATGGTGGCCACATCTCCATGTCCAATCTGACACTCTTGGTG
  AAGAGAGAAGACAATGGCCTGTCCCTCACCTGCGAAGCCTTCAGTGATGCCTTCAGCAAGGAGACCTTCA
  AGAAGTCACTCACCTTGAATGTAAAATACCCGGCCCAGAAGCTGTGGATTGAGGGACCCCCAGAGGGGCA
  GAGCATCCGGACTGGAACTCGGGTGAGGCTGGTATGCTTGGCCATTGGAGGCAACCCAGAGCCCTCCCTC
  ACCTGGCTTAAGGATTCGCGCCCGGTGAACGATCCTCGGCAGTCTCAGGAGCCCCGGCGTGTGCAGCTGG
  GCAGTGTGGAGAAGTCCGGCAGCACTTTCTCCCGCGAGCTGGTGCTGATCATAGGCCCGCCGGACAACCT
  AGCCAAGTTCTCCTGCAAGGCGGGTCAGCTCAGTGCGTCTACGCAGCTGGTGGTGCAGTTCCCCCCAACA
  AACCTGACCATCCTGGCCAACTCGTCCGCACTGCGCCCAGGCGACGCCTTGAACTTGACCTGCGTCAGCA
  TCAGCAGCAACCCTCCAGTTAACTTGTCTTTGGACAAGGAAGGAGAGAGGCTGGACGACGTGGCTGCAAA
  ACCCCAGAGCGCCCCATTCAAAGGCTCCGCTGCATCCAGGAGTGTTTTTCTTCGGGTGTCATCCCGAGAC
  CACGGTCACCGGGTTACCTGCCGGGCCCACAGCGAGGCACTTCGTGAAACCGTGAGCTCTTTCTACCGCC
  TCAACGTGTTATACCCTCCAGAGTTCCTGGGAGAGCAAGTGCGGGCAGTGACCGTGGTGGAGCAGGGCCA
  GGCACTGCTGCCTGTGTCCGTGTCTGCTAACCCCGCCCCCGAGGCCTTCAACTGGACCTTCCGAGGCTAC
  CGCCTCAGCCCAGCTGGGGGTCCCCGGCACCGCATCCTGTCTGGAGGGGCACTGCAGCTGTGGAATGTAA
  CCCGAGCTGACGATGGCTTTTATCAGCTACACTGCCAGAACTCGGAGGGCACCGCCGAGGCGCTGTTGAA
  GCTGGACGTGCATTATGCTCCCACCATCCGTGCCCTGAAGGACCCTACTGAGGTGAATGTTGGGGGTTCT
  GTGGATATAGTCTGCACCGTCGATGCCAATCCCATCCTCCCAGAGATGTTCAGCTGGGAGAGACTGGGGG
  AAGACGAGGAGGAACTGAACCTGGACGACATGGAGAAGATGTCAAAGGGATCCACAGGGCGTCTGCGGAT
  TCGCCAGGCCAAGCTGTCCCAGGCTGGCGCTTACCAGTGCATCGTGGACAACGGGGTGGCTCCCGCAGCC
  AGAGGACTGGTTCGTCTTGTTGTCCGATTTGCCCCCCAGGTGGATCATCCTACTCCCCTGACGAAAGTGG
  CTGCTGCTGGGGACAGCACCAGCTCAGCCACACTCCACTGCCGTGCCCGGGGGGTCCCCAACATCGACTT
  CACTTGGACCAAAAATGGGGTCCCTCTGGATCTCCAAGACCCCAGGTACACAGAGCACAAGTACCACCAG
  GGTGTGGTCCACAGCAGCCTCCTGACCATTGCTAATGTGTCTGCAGCCCAGGACTATGCCCTCTTCAAAT
  GCACAGCCACCAATGCCCTTGGTTCAGACCACACCAACATCCAGCTCGTCAGCATCAGCCGTCCTGACCC
  TCCACTGGGGCTGAAGGTTGTGAGCGTGAGCCCTCACTCGGTAGGACTGGAGTGGAAACCTGGCTTTGAT
  GGGGGTCTGCCTCAGAGGTTCCAAATCAGGTATGAGGCCCTCGAGAGCCCAGGATTCCTCTACATGGATG
  TCCTGCCTGCACAGGCCACCACCTTCACACTGACTGGGCTGAAGCCTTCTACACGGTACAGGATCTGGCT
  GTTGGCCAGCAATGCCCTGGGGGACAGTGGATTGACGGACAAGGGGATCCAGGTCTCCATCACTACCCCA
  GGCCTGGACCAGGCTCCTGAAGACACAGACCAGCCGCTGCCCACAGAGCAGCCTCCGGGACCCCCGAGGC
  TGCCCCTGCTGCCTGTGCTCTTTGCGGTTGGTGGTCTTCTGTTGCTTTCCAATGCCTCCTGTGTTGGGGG
  ACTCCTCTGGCGGAGAAGACTGAGGCGCCTTGCTGAGGAGATCTCAGAGAAGACAGAGGCAGGGTCGGAG
  GAGGATCGAATCAGGAATGAATATGAGGAGAGTCAGTGGACTGGGGACCGGGACACAAGAAGCTCCACGG
  TTAGCACAGCAGAAGTGGACCCACACTACTACTCCATGAGGGACTTCAGTCCCCAGCTTCCCCCAACACT
  GGAAGAGGTGTCATATCGCCAAGCCTTCACAGGTATTGAAGATGAGGACATGGCCTTCCCCGGACACCTG
  TATGACGAGGTGGAGAGAGTCTATGGCCCACCTGGGGTCTGGGGACCCCTCTATGATGAAGTACAAATGG
  ACCCCTATGACCTTCGCTGGCCTGAGGTCAAATATGAGGACCCAAGGGGAATCTACGACCAGGTGGCGGC
  AGACATGGATGCTGGGGAGCCTGGTTCTCTGCCCTTTGAGCTGAGGGGACATCTGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019459
• Insert Size: 3771 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019459.2, NP_062332.2
• RefSeq Size: 5661 bp
• RefSeq ORF: 3771 bp
• Locus ID: 54631
• UniProt ID: Q9QZS7
• Cytogenetics: 7 B1
• Gene Summary: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.[UniProtKB/Swiss-Prot Function]