Taok2 (NM_001163775) Mouse Untagged Clone

CAT#: MC224047

Taok2 (untagged) - Mouse TAO kinase 2 (Taok2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taok2
• Synonyms: 1110033K02Rik; B230344N16; MAP3K17; mKIAA0881; PSK; PSK1; TAO1; TAO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224047 representing NM_001163775
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCTGATGTGGCTGAGCTCTTCTTCAAGGATGACC
  CTGAGAAGCTCTTCTCTGACCTCCGGGAGATCGGCCATGGCAGCTTTGGAGCAGTGTACTTTGCCCGGGA
  TGTCCGGAACAGTGAGGTGGTGGCCATCAAGAAGATGTCCTATAGTGGGAAGCAATCAAATGAGAAATGG
  CAGGATATCATCAAGGAAGTGCGGTTCTTACAGAAGCTACGGCATCCTAATACCATTCAGTACCGGGGCT
  GTTACCTGAGGGAGCACACAGCTTGGCTGGTGATGGAGTATTGCCTGGGCTCAGCTTCTGATCTTCTAGA
  AGTGCACAAGAAACCCCTGCAGGAGGTAGAGATTGCAGCTGTGACCCATGGGGCTCTTCAGGGCCTGGCA
  TATCTACACTCACACAACATGATCCATAGAGATGTGAAGGCTGGAAACATCTTGCTGTCAGAACCAGGCT
  TGGTAAAACTGGGGGACTTTGGCTCTGCGTCAATCATGGCACCTGCCAACTCTTTTGTGGGTACTCCATA
  CTGGATGGCTCCAGAGGTGATCCTAGCCATGGATGAGGGACAATATGATGGCAAAGTGGATGTCTGGTCC
  TTGGGGATAACCTGTATTGAGCTAGCGGAGCGGAAGCCACCACTGTTCAACATGAATGCAATGAGTGCCT
  TATACCACATTGCACAGAATGAATCTCCTGCTCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT
  TGTTGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTTTTGAAGCACCGCTTT
  GTGCTCCGGGAGCGACCGCCCACAGTCATCATGGACCTAATCCAGAGGACCAAGGATGCTGTACGGGAAC
  TGGATAACCTGCAGTACCGAAAGATGAAGAAGATACTGTTCCAAGAGGCACCCAATGGCCCTGGTGCTGA
  GGCCCCAGAGGAAGAGGAGCTCACACCCTGTTCCCAGGAGGCAGAACCTTACACGCACCGTGCAGGGACA
  CTGACCAGTCTAGAGAGCAGCCATTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCAG
  TCAACAGCCTAGCAGATGCCTCAGATAATGAAGAAGAGGAGGAAGAAGAAGAGGAAGAGGAGGAGGAGGA
  GGAGGAAGAAGGCCCTGAATCCAGAGAGATGGCCATGATGCAGGAGGGGGAGCATACAGTCACTTCCCAC
  AGCTCCATCATCCACCGGCTGCCGGGCTCAGACAACCTATATGATGATCCCTACCAGCCAGAGATGACCC
  CAGGTCCACTCCAGCCACCTGCAGCCCCTCCCACCTCCACCTCCTCTTCTGCTCGCCGCAGAGCTTATTG
  CCGCAACCGAGACCACTTTGCTACCATTCGTACTGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAG
  CAGGACTCAGCCCTGCGAGAGCAGCTGAGTGGCTACAAGCGGATGCGACGTCAGCACCAGAAGCAACTGC
  TGGCCCTGGAGTCCCGTCTGAGGGGTGAACGTGAGGAGCACAGTGGGCGGTTACAGCGGGAGCTCGAGGC
  ACAGCGGGCTGGCTTTGGGACCGAGGCTGAGAAGCTGGCCCGGAGGCACCAGGCCATTGGTGAGAAGGAG
  GCACGGGCTGCTCAGGCGGAGGAGCGGAAGTTCCAGCAGCACATCCTGGGGCAGCAGAAGAAGGAGCTGG
  CCGCCCTGCTGGAGGCGCAGAAGCGAACCTACAAGCTGCGGAAGGAGCAGTTGAAAGAGGAGCTCCAGGA
  GAACCCTAGCACACCCAAACGAGAGAAGGCTGAGTGGCTGTTGAGGCAAAAGGAGCAGTTGCAACAGTGC
  CAGGCAGAAGAGGAAGCGGGGCTGCTGCGGAGGCAGCGCCAGTACTTTGAACTTCAGTGTCGCCAATACA
  AGCGCAAGATGCTACTGGCTCGGCACAGCCTAGATCAGGACCTGCTTCGAGAGGACTTGAATAAGAAACA
  GACACAGAAGGACTTGGAGTGTGCTCTGCTGTTACGGCAGCATGAGGCTACCCGAGAGCTGGAGCTAAGA
  CAGCTCCAGGCTGTCCAGCGCACACGTGCTGAACTTACCCGCCTTCAGCACCAGACAGAACTAGGCAACC
  AGCTGGAGTACAACAAGCGACGGGAGCAAGAATTACGGCAGAAGCACGCGGCCCAGGTTCGCCAGCAGCC
  CAAGAGCCTCAAAGTACGTGCAGGCCAGCTACCCATGGGCCTCCCTGCTACTGGGGCTCTGGGACCACTC
  AGCACAGGCACCCCTAGTGAAGAGCAGCCTTGCTCATCTGGCCAGGAGGCAATCCTGGACCAAAGGATGC
  TGGGAGAGGAGGAGGAAGCAGTGCCAGAGAGAAGGATTCTGGGAAAGGAAGGGACTACCTTGGAGCCAGA
  GGAGCAGAGGATTCTGGGGGAAGAAATGGGAACCTTTAGTTCCAGCCCACAAAAACATAGGAGTCTGGCT
  AATGAGGAAGATTGGGATATATCTGAGGAGATGAAGGAGATTAGAGTCCCATCACTGGCATCCCAGGAGA
  GAAATATTATTGGCCAGGAAGAGGCAGCGGCATGGAGTCTGTGGGAGAAGGAGGGTGGGAACCTTGTGGA
  TGTGGAGTTCAAGCTTGGCTGGGTCCAGGGTCCAGTTCTGACTCCAGTCCCGGAGGAGGAAGAGGAGGAG
  GAAGAAGAGGGAGGGGCTCCAATTGGAACCCACAGGGACCCTGGAGATGGCTGTCCTTCCCCAGATATCC
  CCCCAGAGCCACCTCCATCACATCTGAGACAGTACCCTACTAGCCAGCTCCCTGGACTCCTGTCTCATGG
  CCTCCTGGCTGGCCTATCCTTTGCAGTGGGGTCCTCCTCTGGCCTCCTGCCCCTACTCCTCCTGCTGCTA
  CTCCCATTGCTGGCAGCCCAGGGTGGAGGTGGCTTGCAGGCAGCACTGCTGGCCCTTGAGGTAGGGCTAG
  TGGGCCTGGGGGCCTCCTACCTGTTCCTTTGTACAGCTCTACACCTGCCCCCCGGTCTGTTCTTACTCCT
  GGCTCAGGGTACTGCACTGTTGGCTGTCCTTAGCCTGAGCTGGCGCAGAGGCCTTATGGGTGTGCCTCTG
  GGCCTTGGGGCTGCCTGGCTCCTAGCTTGGCCCAGCCTGGCTTTACCTCTGGCAGCTATGGCAGCTGGGG
  GCAAATGGGTACGGCAACAAGGCCCCCAGATGCGTCGGGGTATCTCTCGACTCTGGTTGAGGATTTTGCT
  ACGCCTGTCACCCATGGTCTTTCGGGCCCTACAGGGCTGTGGGGCTGTAGGAGACCGGGGGCTGTTTGCC
  CTGTACCCTAAGACCAATAAGAATGGTTTCCGAAGTCGACTACCTGTCCCTTGGCCCCGTCAGGGAAATC
  CTCGCACTACTCAACACCCCCTAGCTCAGTTAACAAGAGTTTGGGCTGTGTGCAAGGGCTGGAACTGGCG
  CCTAGCACGGGCTAGCCACAGATTAGCTTCTTGCTTGCCCCCCTGGGCTGTTCATATACTAGCCAGCTGG
  GGCCTGCTTAAGGGTGAACGGCCTAGTCGGATCCCTCGGCTGCTGCCACGCAGCCAACGCCGTCTTGGGC
  TCTCTGCTTCCCGACAGCTACCACCAGGGACTGTAGCTGGGCGGAGATCTCAGACTCGCAGGACCCTGCC
  TCCCTGGAGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163775
• Insert Size: 3723 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163775.1, NP_001157247.1
• RefSeq Size: 5270 bp
• RefSeq ORF: 3723 bp
• Locus ID: 381921
• UniProt ID: Q6ZQ29
• Cytogenetics: 7 F3
• Gene Summary: Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. May affect microtubule organization and stability. May play a role in the osmotic stress-MAPK8 pathway. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14/p38 MAPK activation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.