Trim66 (NM_181853) Mouse Untagged Clone

CAT#: MC224046

Trim66 (untagged) - Mouse tripartite motif-containing 66 (Trim66), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim66
• Synonyms: Kiaa0298-hp; Tif1d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224046 representing NM_181853
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGAACTGCTCTGAGTGCAAGGAGAAGCGGGCAGCACACATCCTCTGCACATACTGCAACCGCT
  GGCTGTGCAGCTCCTGCACAGAGGAGCACCGGCATGTCCCAGCCCCGGGGGGCCCACTCTTTGCACGGGC
  ACAGAAGGGATCCTCAGGGGTGAATGGTGGCTCTGGGGACTTTGCCTTGTACTGCCCTCTGCACACACAG
  GAAGTGCTCAAGCTTTTCTGCGAGACGTGCGATGTACTCACCTGCCACAGCTGCCTGATGGTGGAGCACA
  AAGAGCACAGATGCAGGCACGTGGAAGAAGTTCTACAAAACCAGAGGATGCTCCTGGAGAGTGTGACTTC
  CCAGGTGGCACATAAGAAATCCAGCCTACAGACATCTGCAAAGCAAATCGAGGACAGAATTTTTGAAGTG
  AAGCATCAGCACCGGAAGGTGGAGAACCAGATTAAAATGGCCAAGATGGTCCTCATGAACGAGTTGAACA
  AGCAAGCCAACGGGCTCATAGAGGAGCTTGAGGGCATCACTAATGAGAGAAAGCGAAAGCTGGAGCAGCA
  GCTGCAGAGCATCATGGTTCTCAACCGGCAGTTTGAACATGTGCAGAATTTCATCAACTGGGCTGTCTGC
  AGCAAAAGCAGCGTCCCTTTCCTTTTCAGCAAAGAGCTGATTGTGTTTCAGATGCAGCGGTTGCTGGAGA
  CGAGATGTAACACAGATCCTGGCTCTCCTTGGAGTATCAGATTCACCTGGGAGCCCAACTTCTGGACCAA
  GCAGCTGGCTTCCCTTGGCTGCATAACCACTGAAGGTGGACAGCTGACCCGGGCAGATGCTGCAGCTGCC
  TCTTATGGGAGTTTACAGGGGCAGCCATCTTTTTATCAGAGCCACCAGGCCCCCATGGCTCAGCAGGAGG
  CACTCAGCCACCCCTCACACAAGTTCCAGTCTCCAGCACTGTGTTCCTCGTCTGTCTGCTGCTCCCACTG
  CTCTCCAGTCTCGCCTTCCCTCAAAGGTCAAGTCCCTCCACCCAGCATCCACCCGGCTCACAGTTTCCGA
  CAGCCTTCTGAAATGGTGCCCCACCAGCTGGGGTCACTGCAGTGTTCTACCCTGCTGCCCAGGGAGAAAG
  AGCTGGCCTGCAGTCCTCATCCACCCAAACTGATGCAGCCCTGGTTGGAACCACAACCTCCTGCAGAACA
  GGAGAGTACATCCCAGCGGCCAGGACCGCAACTGGTTTCCCAGCCTGTGTGCATCGTCCCTCCGCAAGAT
  GTGCAGCCAGGAGCCCATGCCCAGCCCACCATACAGACACCCTCCATCCAAGTTCAGCTGGGCCACCATC
  AGAAGCTAAAGCTCAGCCACTTTCAGCAGCAGCCGCAGCAGCAGCCACCCCCACCACCGCCACCTCCCCC
  ACCCCCCCAGCATGCACCTCCACCCTTGCCTCCATCCCAGCACCTGGCTTCCAGTCAGCACGAGAGCCCT
  CCTGGGCCTGCCTGTTCGCAGAATGTGGACATTATGCACCACAAGTTTGAGCTGGAGGAGATGCAGAAGG
  ACTTGGAGCTTCTCCTGCAGGCCCAGCAGCCTAGCCTGCAGTTGAGTCAGACCAAGTCTCCTCAGCATCT
  TCAGCAAACCATCGTGGGGCAGATCAACTACATCGTGAGGCAGCCAGCACCCGTGCAGTCCCAGAGCCAG
  GAGGAGACCCTGCAGGTTACAGAGGAGCCACCAGCACCTGAGGGCCCAAAGCCAGCTCTACCTGTTGACA
  AGAACACTGCTGCTCCCTTGCCCCAGACATCTGGGGAGGAAACGCCTCACAGTGTCCCTCCAGTGGATGG
  CACCTCCCAGCACTCTTCTCCAAATGTGGTGAGAAAGCACGCCACCTCAGTGAGCATCATGGGCTTTTCC
  AACACTGTGGAGATGGAGTTGTCATCTACCAGGCTGGCGAGGACCATAGAGCCACAGATACACAGGGTGA
  GCAGCCTGACAGCTGCCCCCACCCACACAATTCCGAGCTTGCTGAGTGGCCCACCACAAACTGTGTCCAG
  CCTGATGAGTGTTTCAAACCATGCCATGCCAAGCCTGACAGCCAGTCACCTGCAGCCCGTGCCAAACCTT
  GTGCGTGGCACATTCCAGTCCACGTCGAACCTGAGGGGTGATTCCTCTCAAGCCATCACAGGCCTGGCAA
  GTAATCACTCACAAGCTGGGCCCAGCCTAATGTCTGGGCACACCCAGGCTGCGCCGAGTCTGGCCACTTG
  TCCTCTGCAGGGAATGCCACCGGTTTCTGATGTGCATGTGGAGCCGAGATCCGTATCCAGTCCTGGGTCT
  GGCCCAGCTGCAGAAAGCCTGGGTACCAGGGATGGGGCTGAGTCCTCCCTGGGGAATGCCTTGTGTAAGA
  TGGAAAGTGAGGATTGTACCCGTTTCTCAGACTCAGTAGGACAAGGCCCTACAGCCTCCAGTCTGGATGG
  TCCCAAGGATTTGGCTATTCCCTCAGAACTGGAGGAACCAATTAACCTCTCTGTGAAAAAACCTTTCCTG
  GCACCAGTGATCAACACATCCACTGCTCTGCAACAGTACCGGAATCCAAAAGAATATGAGAATTTTGAAC
  AAGGAGCCCTAGAGCTGGATACAAAGGAGAATTCAGACATCAGAGCTATCAGTAGTGAGCCCAAGATCCC
  CTACGTGCGACTGGAGCGGCTCAAGATCTGTGCTGCCTCATCAGGAGAGATGCCTGTGTTTAAGCTGAAG
  CCCCAGAAAAATAGCCAGGACGGAAACTTCCTCCTGGTGATTGAGTGTGGCACTGAGTCCTCCAGCATGT
  CCATTAAGGTCAGCCAGAACAGCCTGCCTGATGCCAGCCAGGGCCCAGGTCTTGGGGGAAGAAAGGTCAC
  TGTCACATCTCTGACTGGGCAGCAGCCACAAGAGGTGGAGAGCACATCTGAGGAACACAGACTCATCCCC
  CGAGCTCCAGGAGCCAAGAAGAACACCCCGGCCCCCATAGAGAATGAGGACTTCTGTGCGGTGTGCATCA
  ACGGTGGGGAGCTGCTATGCTGTGACCGCTGCCCCAAAGTGTATCACCTTTCCTGCCACGTGCCAGCCTT
  GCTCAGCTTCCCAGGGGGAGAATGGGTGTGCACCCTGTGCCGCAGCCTGACGCAGCCTGAGATGGAGTAC
  GACTGTGAGAACGCGCGCTACGGTCACCCTGGAGTGCGGGTACTTCCCGGCCTGAGCATGTATGACCAGA
  AGAAGTGTGAGAAGCTGGTCCTGTCCCTGTGCTGCAACAGCCTCAGCCTGCCCTTCCATGAACCAGTCAG
  CCCTCTGGCCAGGCATTATTACCAGATTATCAAGAGGCCCATGGACCTGTCAATCATCCGGAGGAAACTG
  CAAAAGAAGGACCCAGCTCATTACACTACTCCAGAGGAAGTGGTGTCAGATGTACGCCTCATGTTCTGGA
  ACTGTGCTAAGTTCAATTATCCTGACTCGGAGGTTGCAGAGGCTGGCCGCTGTCTGGAAGTGTTCTTTGA
  AGGCTGGCTAAAGGAGATCTACCCAGACAAATGCTTTGCCCAGCCCCAGCAAGAGGATTCAGACTCTGAG
  GACGTATCTGGCGAGAGTGGCTGTTCCACTCCTCAGGGCTTCCCATGGCCTCCCTACATGCAGGAAGGCA
  TCCAGCCCAAGAGGCGACGGCGGCATATGGAGAATGAGAAGACAAAGAGAGTGTCGTTTCGTCTGGCCAA
  CAGCATCTCGCAGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181853
• Insert Size: 3729 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181853.4, NP_862901.3
• RefSeq Size: 9129 bp
• RefSeq ORF: 3729 bp
• Locus ID: 330627
• UniProt ID: Q924W6
• Cytogenetics: 7 57.21 cM
• Gene Summary: May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.