Ttbk2 (NM_001024856) Mouse Untagged Clone

CAT#: MC224033

Ttbk2 (untagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttbk2
• Synonyms: 2610507N02Rik; AI326283; B930008N24Rik; mKIAA0847; Ttbk; Ttbk1; TTK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224033 representing NM_001024856
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGT
  TAAGAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGT
  GGCGCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAA
  CTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCA
  TGCAATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCAC
  TACCCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGA
  GACATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATT
  TTGGCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCG
  AGGGACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGG
  TCTTTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGC
  AAGTAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCAC
  CTTTCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTT
  GACAATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATG
  GCTCCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGG
  AATTGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAA
  CAGCTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGC
  ACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAAT
  TTGCAAAGCAGCTACTGAAGAAGAAAATAGCCATGGTCAAGTAAATGGCATACTCAATGCTCCAAGCCTT
  GGTTCACCAATTCGTGTCCGATCAGAGATTACTCAGCCAGACAGAGATGTTCCGTTAGTAAGGAAGCTAC
  GTTCTATTCACAGCTTTGAGCTGGAAAAACGTTTGACACTTGAGCCAAAGCCAGATACTGACAAGTTTCT
  GGAGACCTGCATGGAGAAAATGCAGAAAGATTCCAGTGCAGGAAAAGAACCTGTCCCCCCTGCTCTGCCT
  CATAAGCCGTGTGTCCCCGTCGTGACCCACACTGACCACATCTGGCACTATGATGACGAATATCTTCCTG
  ATGCCTCCAAGCCTGCCTCTGCCAACACCCCGGAGCAGGCAGATGGTGGTGGCAGCAACGGATTTATAGC
  TGTTAACTTAAGCTCTTGCAAACAGGAGGTTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAAGAC
  CTTCAGGACTTTAGGACAAATGAGGTGTTAGGCCATAAGACAACTGGAAGCCCTTCAGATGAGGAGCCTG
  AAGTGCTTCAGGTCCTTGAGGGATCACCTCAAGATGAAAAGATCCAAGTAGGTCCTTGGACTGACAACCA
  TCACTTAAAGAAGGAAAGCTCAGGTGTGGTTTTAGCACTTTCTGCTGAATGCCCTGCTACTGCTGCTTCA
  GAACTGTACACAGATAGGCTAGACCTCCAGGCTGGAGCTGCAAGTCAGTTCATCACAGTGACTCCCACAA
  GTCCCATGGAGGCACAAGCAGAAGGACCCCTGACTGCGATTACAATTCCTAGACCTTCTGTGGCATCAAC
  ACAGTCAACTTCAGGAAGCTTTCACTATGGCCCACAACCAGAGAAGAAAGATCTTCAGCCCTTGGAGCCC
  ACTGTAGAACTGTACTCTCCAAGGGAGAACTTCTCCGGCTTGGTTGTGACAGAGGGTGAACTAGCTAGCG
  GAGGAAGCAGAGTGGATTTGGGACTTCAGATAGATCACACTGGTCATGACATGTTACCCAACATGAGAGA
  TGGTGACACATCTCAAGACTTGGGACCAAAAGACCCTCCTGACCATAATAGATTAGCTGTGAAAGAATTT
  GAACATCTCCCTGGAGAAACAGAAGAGAGAAGCCTTCTTCTGGGCTCAGAGAATGAAGATGAGAGGTTAA
  GTAAAGGGCAGCACTGTATTGAAGTCTCTTCCCCAGGAGAGTTAGTGACTGCAGAGAGGGCTCAGTTAGC
  TGCCACAGAACCTCTGCATGTATCAGAGACACAGAACTGTAGTGTGCTACCAAATCAGGACAAAACCCAT
  GAGATAATGAAGCTTTTGGCAGTTGGAACTTCAGAAATTTCTCCTCAAGCCATTGACCCACATGCTGAAG
  GGCAGATAGGCCAGATGGCAGCAATGCAGAAAAATAAGCTATTTAAAGATGATGGTATTCAGAGTGAAAG
  CTTGCCAAGGCAGCAGGGAGACCTCTCTGCTTTTTTGCACCAAGAGGGTAAGAGAGAGAAAGTTGTCCCT
  AGAAATGGAGAGCTCTATCATTGTGTCTCAGAGAATGAGCATGGTCCTCCTACTCGGAAGGACATGCTCC
  GGTCATCCTTTGTGACCAGGCACAGCCGGATCCCTGTTTTAGCACAAGAAATAGACTCAACTTTTGAATC
  ATCCTCTGCTATTTCTGCAAAAGAAAAGCTTCTACAGAAGAAAGCCTATCAGCCAGAAATAGTCAAACTT
  CTTGTAGAAAAAAGGCAGTTCAAGTCTTTCCTGGGAGACCTCTCAAGTGCCTCTGATAAGCTGATAGAGG
  AGAAACTAGCCGCTGTTCCAGTCCCTTTTTCTGAGGAGGAAGTCTTCGCTCCCTTTTCTAGACTGGCAGC
  AGATTCCCACCTGAGTAGATCAGTTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGGCTAGAAAGAGC
  AAGATTCCAAGGCCAGTATCCTGGGTCAGCACAGATCAAATTAATGGCTCCGCTTCACCTCAGTTCTTGC
  CTCGGCCACCACCAGGAAAGCCACCAGTCAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTTCT
  AGGGAGTAGTAACTCTGACTCAGACCTTTTCTCTCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAA
  TCCCGGAGTACTACCCAATGCAAGAGCCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAG
  TTGTACCTCGAAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGAACATCTAGTTCCTCACC
  ATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCTTCCCCACATCTGGGGAGAAGCAAGTCACCC
  CCTAGCCACTCAGGATCATCGTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATG
  GCCCAAAAGGATCTGGCAGCCTCCACCACCACTCAACCAGCTCTAAAACTCCCCCAGGGAAGAGTAAGCC
  AGCCAGTAAACTCAGCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024856
• Insert Size: 3732 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024856.2, NP_001020027.1
• RefSeq Size: 11152 bp
• RefSeq ORF: 3732 bp
• Locus ID: 140810
• UniProt ID: Q3UVR3
• Cytogenetics: 2 E5
• Gene Summary: Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has a different 5' end exon compared to transcript variant 1. It encodes an isoform (2) that is shorter and with a distinct N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform.