Wnk4 (NM_175638) Mouse Untagged Clone

CAT#: MC223989

Wnk4 (untagged) - Mouse WNK lysine deficient protein kinase 4 (Wnk4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wnk4
• Synonyms: 2010002J11Rik; Pha2b; Prkwnk4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223989 representing NM_175638
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGCACCTCGAAATACGGAGACTGGGGTCCCCATGTCCCAAACCGAGGCTGACCTGGCTCTGCGGC
  CATCTCCGGCTCTCACCTCTACGGGGCCGACCCGCCTGGGGCCACCTCCTCGCCGAGTGCGCCGCTTCTC
  TGGGAAGGCTGAGCCTCGGCCGCGCTCTTCGAGACCTAGCCGCCGCAGCTCAGTCGATCTGGGACTGCTG
  AGCTCCTGGTCTCAACCAGCCTCACTCCTTCCGGAACCCCCGGATCCTCCAGACTCCGCTGGCCCCACGA
  GGAGCCCACCTTCAAGCTCTAAAGAACCCCCCGAGGGCACATGGATGGGGGCAGCTCCCGTGAAGGCTGT
  GGACTCTGCATGTCCTGAGCTTACGGGATCTTCAGGAGGTCCAGGGTCCCGGGAGCCACCGAGGGTTCCT
  GATGCTGCAGCCCGGGAACGGCGGCGGGAGCAGGAAGAAAAAGAGGACACAGAAACCCAGGCTGTGGCAA
  CGTCTCCGGACGGCCGATACCTCAAGTTTGACATTGAGATTGGAAGAGGTTCCTTCAAGACCGTGTATCG
  AGGGCTAGACACCGACACCACGGTAGAGGTGGCCTGGTGTGAGCTGCAGACCCGGAAACTCTCTCGGGCC
  GAGCGGCAGCGTTTCTCCGAGGAAGTTGAGATGCTCAAGGGGCTGCAGCACCCCAACATCGTCCGCTTCT
  ATGACTCCTGGAAGTCGGTGCTGAGGGGCCAGGTTTGCATCGTGCTGGTCACAGAACTCATGACCTCGGG
  CACGCTCAAGACGTACCTGAGGCGGTTCCGCGAAATGAAGCCCCGAGTCCTTCAGCGCTGGAGTCGCCAG
  ATTCTACGGGGACTTCATTTCCTTCATTCCCGAGTGCCCCCCATCCTGCACCGAGATCTCAAGTGTGACA
  ACGTCTTCATTACTGGCCCTTCAGGCTCTGTCAAAATCGGAGACCTCGGACTGGCCACGCTCAAGCGCGC
  CTCCTTTGCCAAGAGCGTCATTGGGACCCCGGAATTCATGGCTCCTGAGATGTACGAGGAAAAGTACGAC
  GAGGCTGTGGATGTGTACGCCTTTGGCATGTGCATGCTGGAGATGGCTACGTCTGAGTATCCCTACTCCG
  AGTGTCAGAATGCAGCACAAATCTACCGCAAGGTCACTTCGGGCACAAAGCCCAACAGCTTCTACAAGGT
  GAAGATGCCAGAGGTGAAGGAGATCATTGAAGGCTGCATCCGCACGGATAAGAACGAGAGGTTCACCATC
  CAGGATCTCCTGGCCCATGCGTTCTTCCGCGAGGAACGAGGTGTGCACGTGGAGCTGGCAGAGGAAGATG
  ATGGAGAGAAGCCGGGCCTCAAGCTCTGGCTGCGCATGGAGGATGCGCGGCGCGGGGGGCGTCCACGAGA
  CAACCAGGCCATCGAGTTCCTGTTTCAGCTCGGCCGGGACGCTGCGGAGGAGGTGGCTCAGGAGATGGTA
  GCCTTGGGCTTAGTCTGTGAAGCTGACTACCAGCCAGTGGCCCGTGCAGTTCGAGAACGGGTTGCTGCTA
  TCCAGCGGAAGCGGGAGAAGCTTCGAAAAGCTAGGGAATTGGAGGTTCTCCCACCAGACTCAGGACCTCC
  TCCAGCAACTGTGTCTCTGGCTCCGGGTCCCCCCAGTGCCTTCCCCCCAGAGCCTGAGGAGCCAGAGGCT
  GACCAGCATCAATCCTTCCTCTTCCGCCATGCCAGCTACTCATCAACTACATCTGATTGCGAGACTGATG
  GCTACCTCAGTTCCTCCGGCTTCCTGGATGCCTCAGACCCTGCCCTTCAGCCCCCTGGGGGGTTACCATC
  CAGCCCCGCTGAATCCCATCTCTGCTTGCCCTCGGGTTTTGCCTTGTCTATTCCACGGTCTGGCCCTGGC
  AGTGACTTTTCTCCTGGGGATAGCTACGCCTCAGATGCGGCATCAGGCCTTAGTGACATGGGAGAAGGGG
  GGCAAATGAGGAAAAATCCAGTGAAGACTCTTCGACGGAGACCTCGATCCCGGCTCCGGGTCACTAGTGT
  CTCAGACCAGAGCGACAGAGTTGTCGAGTGTCAGCTGCAGACTCACAACAGCAAGATGGTGACGTTCCGA
  TTTGATCTGGATGGGGACAGCCCAGAAGAAATTGCAGCTGCCATGGTATACAATGAGTTCATCCTGCCCT
  CGGAGCGAGATGGATTCCTGAGCCGGATCCGGGAGATTATCCAGCGAGTGGAGACCCTGCTGAAGAGAGA
  TGCTGGTCCCCCAGAGGCTGCTGAAGATGCCCTGAGCCCCCAGGAAGAGCCAGCAGCCCTGCCTGCCCTC
  CCAGGCCCACCCAATGCAGAGCCCCAGAGAAGCATCTCCCCAGAACAGAGGAGCTGGGCAGCCTTCTCCA
  CCTCTCCATCTTCTCCTGGCACCCCCTTGTCCCCTGGGGCCCCCTTTTCCCCTGGGACCCCTCCTGTCTT
  CCCATGCCCCATCTTTCCTATCACTTCACCCTCATGCTATCCCTGCCCATTCTCCCAGGTCTCTTCAAAC
  CCCTATCCACAGGCCCCCAGTTCCCTGCTTCCCTTATCCTCCAGTGCTTCTCAGGTTCCACTTCCATCTT
  CCTCTCTTCCCATCAGCGCTCCCCTCCCATTCTCTCCTAGTTATCCCCAAGACCCTCTTAGCCCCACTTC
  TCTTCCCGTCTGCCCCTCTCCTCCCTCTCTTCCCTCTACCACAGCAGCCCCTCTCCTCTCTCTGGCTAGT
  GCCTTCTCTCTGGCTGTGATGACTGTGGCCCAGTCCCTGCTGTCCCCATCCCCTGGACTTCTTTCTCAGT
  CTCCTCCAGCCCCTCCAGGTCCTCTGCCTAGCCTGCCCCTTTCCCTTGCTTCTTGTGACCAGGAGAGCCT
  TTCGGCCCAAACAGCTGAGACAGAGAATGAGGCTTCCCGTAATCCTGCTCAGCCACTACTGGGTGATGCT
  AGACTGGCACCTATATCTGAAGAGGGAAAGCCCCAGCTTGTTGGCCGTTTCCAAGTGACTTCATCTAAGG
  AACCAGCAGAGCCTCCCCTGCAACCAGCATCCCCAACTCTCTCCAGATCCCTAAAGCTGCCAAGCCCTCC
  GCTGACCTCAGAGAGCTCAGACACAGAGGACAGTGCTGCAGGAGGCCCAGAGACTAGGGAGGCTCTGGCA
  GAGAGTGACCGTGCAGCCGAAGGCCTGGGGGTTGCGGTCGATGATGAAAAGGATGAAGGGAAGGAACCCC
  TACTTGGAGGCAGTTCCCCAATCTTGAGCCATCCCAGCCCAGTGTGGATGAACTACTCCTACAGCAGCCT
  GTGTCTGAGCAGTGAGGAGTCGGAGAGCAGCGGGGAAGACGAGGAATTCTGGGCTGAGCTGCAGAACCTT
  CGGCAGAAGCACTTGTCGGAAGTGGAGGCACTACAGACACTACAGAAGAAGGAAATCGAGGACTTATACA
  GCCGGCTTGGGAAACAGCCCCCACCTGGTATTGTAGCTCCAGCTGCTATGCTGTCCTGCCGCCAGCGCCG
  CCTCTCGAAGGGCAGCTTTCCCACCTCCCGCCGCAACAGCCTGCAGCGCTCTGATCTCCCTGGTCCTGGC
  ATCATGCGAAGGAACTCTCTGAGTGGCAGCAGCACCGGCTCCCAGGAGCAGCGGGCAAGCAAGGGGGTGA
  CATTCGCCGGGGATATTGGCAGGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175638
• Insert Size: 3669 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175638.3, NP_783569.1
• RefSeq Size: 4148 bp
• RefSeq ORF: 3669 bp
• Locus ID: 69847
• UniProt ID: Q80UE6
• Cytogenetics: 11 D
• Gene Summary: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537).[UniProtKB/Swiss-Prot Function]