Tbcd (NM_029878) Mouse Untagged Clone

CAT#: MC223905

Tbcd (untagged) - Mouse tubulin-specific chaperone d (Tbcd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbcd
• Synonyms: 2310057L06Rik; A030005L14Rik; mKIAA0988
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223905 representing NM_029878
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACTGAGCAATGAGCCGGCTGCGAGCGCCGCCGAGGAGGAGGTGGAGGATGATGCGCTTGTTCGCG
  CCTCCGCCCTGGAAGCGTTCGGCGAGAGTGCGGAGACCCGCGCGCTGCTCCGCAGCTTGCCGGCGGTGCA
  CAGAGAGCGCGCGTCCCGGGAGGTGGCGGAGGAGCGGTTCCGCGTAATTATGGACAAATACCAGGAGCAG
  CCCCACCTCCTGGATCCACACCTTGAGTGGATGATGAACTCACTGTTGGACCTGGTACAGGATGAGACAT
  CTCTGCCCGACCTTGTCCATCTGGCTTTTAAGTTTCTTTATATCATCACCAAGGTTCGTGGATATAAAGT
  GTTTCTGCGTCTGTTTCCTCACGAAGTGGCTAATGTTCAGCCTGTGTTAGATATGTTCACAGGTCAGAAC
  CCTAAAGACCATGAGACGTGGGAAACACGCTACATGCTTCTACTGTGGCTCTCTGTGACCTGCTTGATCC
  CTTTTGATTTTTCACGCCTTGATGGGAACCTGTCTACTCAAACTGGAGAAACACGAGTACCCACCATGGA
  CCGAATTCTGCAAATAGCAGAGTCTTACTTGGTGGTCAGTGACAAGGCGCGAGATGCAGCTGCTGTCCTT
  GTGTCCAAATTTATCACACGTCCCGATGTCAAGCAGAGAAAGATGGCCAGCTTTCTGGACTGGAGCCTTT
  GCACCTTGGCCCACTCTTCCTTCCAGACCATCGAGGGTGTCATTACCATGGATGGGATGCTGCAGGCCCT
  GGCTCAAATATTTAAGCATGGAAAACGTGAAGACTGCCTTCCCTATGCCAACACTGTGCTCCAGTGCCTC
  GACGGCTGCAGACTCCCCGAGAGCAGTCATACCTCGCTGCGGAAGCTTGGTGTGAAGCTTGTCCAGAGGC
  TGGGGCTGACTTTCCTGAAGCCAAAGGTGGCCACGTGGAGGTATCAGCGTGGCTGTCGGTCTTTGGCTGC
  TAACCTGAAGCTCTGTGCCCCGGGTAAGAGTGACCAGAAGCTGCTGTCTGATAGTTTGACCTCTGATGGT
  GATGAAGACTATGATGTCCCAGAGGGGGTAGAAACTGTGATAGAGCAGCTACTGGTTGGGTTGAAGGACA
  AGGACACTGTGGTGCGGTGGTCTGCAGCGAAAGGAATTGGTAGGATGGCTGGAAGGCTTCCCAGAGAGCT
  GGCCGATGATGTGGTGGGGTCTGTGTTGGACTGTTTCAGTTTTCAGGAGACGGATAAGGCATGGCATGGT
  GGATGTCTGGCATTGGCAGAGCTGGGTAGACGAGGCCTGTTGCTACCATCCAGACTCTCAGAGGTTGTCA
  CGGTGATCCTGAAGGCCCTTACCTACGATGAGAAGCGGGGTGCCTGCAGCGTTGGGGCCAATGTCAGGGA
  TGCTGCCTGTTATGTGTGCTGGGCTTTTGCACGTGCCTATGAACCTCAGGAGTTGACACCTTTTGTGACT
  GCCATTTCAAGTGCCCTGGTCATTGCTGCTGTATTTGACCGAAATGTGAACTGCAGAAGAGCAGCCTCTG
  CTGCCTTCCAGGAGAACGTGGGGAGACAGGGTACTTTCCCTCATGGAATTGATATTTTGACTACAGCAGA
  CTATTTTGCAGTTGGTAACATATCTAACTGTTTCCTGATTATAAGTGTGTTTATTGCTGGCTTCCAGGAA
  TACACCAAGCCCATGATTGATCACCTGGTCTCCATGAAAATCAACCATTGGGATGGGGCCATCCGAGAAC
  TGTCTGCAAAGGCATTGCACAACCTGACTCCACAAGTACCCGAGTACATAGCCATGCACGTATTCCCTGC
  ACTACTGTTGATGACACAGAGTCCAGATCTACACACGAGACATGGTGCCATCCTTGCCTGTGCAGAGGTC
  ACCTATGCCCTGTACAAGCTGGCCACACAGAGTAACAGGCTTGTCACAGACTATCTGGATGAGAAGGCAG
  TGCAAAGCCTGAAGCAGATCCACCAGCAGCTTTGTGACCGCCATCTCTATAGGGGTCTTGGAGGAGAGCT
  TATGAGACAAGCAGTGTGCATCTTAATAGAGAAGCTGTCACTTTCCAGAATGCCATTTAAAGGGGACGCC
  ACTGTCGAAGGTTGGCAGTGGCTGATAAATGACACTCTCAGGAGTCTTCACCTTGTTTCAAGCCATTCCA
  GACAACAGATCAAGGAGGTGGCTGTCTCTGCCCTGACTGCCCTCTGCAGTGAATACTACGTGAAGGAGCC
  TGGGGAGGCAGGCTCCTCCATCGCGAAGGAGCTGATTCCCCAGTACCTTGCTGAGCTCCAGAGCCCAGAG
  GAGATGGCCCGTTGTGGCTTTTCCTCAGCCTTGGGTGCCCTCCCAGGTTTCCTTCTCAGGGGCCATCTGC
  AGCAGGTTCTCTCAGGTCTGAGAAGAGTCACCTGTATTTCTCCCAATGATGTGAGCTTTGCTGAAGCCCG
  GAGAGATGGTTTGAAAGCAATCTCAAGAATTTGCCAGACGGTTGGTGTGAACACACGGGGGCCTCCAGAT
  GAAGTCATATGCAAAGAGAACATCTCTGAGGTTTATGCTGCATTGCTGGGCTGCATGAGTGATTACACCA
  CAGACAGCAGAGGGGATGTGGGAGCCTGGGTTCGTGAGGCTGCCATGACCAGCCTCATGGATTTGATGCT
  TTTGCTGGCACGGACAGAGCCGGTGCTGATTGAGGCCCACATCTGTGAGCGCGTCATGTGTTGCGTTGCC
  CAGCAAGCAAGTGAGAAGATTGATCGGTTCCGTGCTCATGCTGCCCGGGTGTTCCTGACTCTCTTGCACT
  TCGACAGCCCTCCCATTCCCCATGTTCCCCACCGCCAAGAACTGGAAAGTCTGTTTCCCAGGTCTGATGT
  GGCCACTGTGAACTGGAACGCACCTTCCCAGGCCTTCCCTCTCATCACCCAGCTCTTGGGATTGCCTACC
  TATAGATACCATGTCCTGCTGGGGCTGGCTGTGTCTGTGGGTGGCTTGACAGAGTCTACGGTCAGACATT
  CTACACAGAGCCTCTTTGAGTACATGAAGGGGATTCAGAAGGATGCTCAGGTCCTGCAGAGCTTCAGTGA
  GACCCTCCTGAAGGTCTTTGAGGACAACCTTTTGAATGATAGGGTGTCTGTATCACTGCTGAAAATGCTG
  GACCAGCTGTTGGCTAATGGGTGCTTTGACATCTTTACTGCCGAGGAGAACCACCCCTTTTGTGTGAAGT
  TGCTCACACTTTGTAAGGAAGAAATCAAGAAGTCAAAAGACATCCAGAAACTTCGGTCAAGCATCGCAGT
  GCTCTGTGGGATGGTGCAGTTCAATGGTGATGTGAGAAAGAAGATTCTTCTTCAGCTGTTCCTCCTCCTC
  GGCCACCCTTTCCCTGTGATCCGGAAGAGCACAGCGAGCCAGGTCTACGAGATGGTGCTTACCTACAGCG
  ACCTGGTAGATGCCGAGGTGCTTGATGAGGTCATGTCTGTGCTCAGTGACACAGCCTGGGACGCAGAGCT
  TCCAGTTGTAAGAGAACAGCGGAATCGCCTGTGTGACCTTCTTGGCGTGCCCAGACCCCAGCTGGTTCCA
  AAGCCTATTCCAGGAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029878
• Insert Size: 3591 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029878.3, NP_084154.1
• RefSeq Size: 3912 bp
• RefSeq ORF: 3591 bp
• Locus ID: 108903
• UniProt ID: Q8BYA0
• Cytogenetics: 11 E2
• Gene Summary: Tubulin-folding protein implicated in the first step of the tubulin folding pathway and required for tubulin complex assembly. Involved in the regulation of microtubule polymerization or depolymerization, it modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Acts as a GTPase-activating protein (GAP) for ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. Required for correct assembly and maintenance of the mitotic spindle, and proper progression of mitosis. Involved in neuron morphogenesis.[UniProtKB/Swiss-Prot Function]