Washc4 (NM_001033375) Mouse Untagged Clone

CAT#: MC223843

A230046K03Rik (untagged) - Mouse RIKEN cDNA A230046K03 gene (A230046K03Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Washc4
• Synonyms: A230046K03Rik; AA589518; Kiaa1033; mKIAA1033
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223843 representing NM_001033375
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGACACTCTGTCGCCCGACTGGGACTTCGACCGCGTGGACGACGGCTCTCAGAAAATTCATG
  CTGAAGTCCAGCTGAAGAACTACGGGAGGTTCCTTGAGGAGTACACATCTCAGCTGAGAAGAATTGAGGA
  CGCCCTAGATGATTTGATTGGCGATGTTTGGGATTTCAATCTTGATCCTATAGCATTAAAGCTTCTGCCC
  TATGAGCAGTCTTCCCTTTTGGAACTCATAAAGACTGAAAACAAGGTCCTGAACAAAGTCATCACTGTTT
  ATGCGGCACTTTGTTGCGAGATCAAGAAATTAAAATATGAGGCAGAAACTAAGTTTTACAATGGTCTTCT
  GTTTTATGGTGAAGGAGCTACAGATTCCAGCATGGTGGAAGGTGACTGCCAAATTCAAATGGGGAGATTC
  GTTTCATTCTTACAGGAACTGTCCTGCTTTGTGACGAGGTGCTACGAAGTGGTGATGAATGTCATCCACC
  AGCTGGCTGCCCTGTACATCAGCAACAAGATAGGACCCAAAATTATAGAGACAACTGGAGTTCATTTTCA
  GACTATGTATGAGCACTTGGGAGAACTGCTAACAGTTTTACTTACCCTGGATGAGATTGTTGATAATCAT
  GTCACGCTGAAAGACCACTGGACTATGTACAAAAGGTTGCTGAAATCTGTCCATCACAACCCTTCAAAAT
  TTGGGATTCAGGAAGAAAAGTTAAAGCCCTTTGAGAAGTTCTTGCTGAAGCTGGAAGGGCAGTTACTTGA
  TGGAATGATATTTCAGGCCTGTATCGAACAGCAGTTTGATTCTCTCAATGGAGGAATATCTGTGTCGAAA
  AATAGCACGTTTGCAGAAGAGTTTGCACATAGCATTCGCTCAATTTTTGCAAATGTGGAAGCCAAGCTTG
  GTGAGCCTTCTGAAATTGACCAGAGAGACAAGTATGTTGGAATTTGTGGGCTCTTTGTACTGCACTTTCA
  GATTTTTCGGACTGTTGATAAAAAGTTTTATAAGTCTTTATTGGACATTTGTAAGAAGGTGCCAGCCATC
  ACTTTAACTGCTAATATTATTTGGTTTCCTGATAATTTTCTGATTCATAAAATGCCAGCAGCTGCCAAAC
  TTCTAGACAGAAAAAGTCTTCAAGCCATTAAAATACACAGGGATACCTTCCTACAGCAGAAAGCTCAGTC
  ACTTAACAAAGATGTGCAGTCTTACTACGTCTTTGTGAGCTCATGGATGATGAAGATGGAGTCTATGTTA
  TCTAAAGAGCAGAGGATGGATACATTTGCTGAAGACCTCACCAACAGATGCAATGTTTTCATACAGGGTT
  TCTTATATGCATATAGTATTAGTACCATTATTAAGACCACAATGAATCTCTACATGTCCATGCAGAAGCC
  TATGACCAAAACTTCAGTGAAGGCATTATGCAGGCTCATTGAACTTCTCAAGGCAATAGAGCATATGTTC
  TACAGGAGAAGCATGGTTGTGGCGGATTCGGTGTCACATATAACACAGCACCTTCAACATCAGGCTCTTA
  GTTCTATTTCTGTAGCCAAGAAAAGAGTAATTTCAGACAAAAAATATAGTGAACAGCGTCTTGATGTGCT
  ATCTGCTCTTGTTTTGGCTGAAAACACCTTAAATGGACCAAGCACAAAACAACGACGACTTATTGTTTCT
  TTGGCATTGAGCGTTGGCACGCAAATGAAAACATTTAAAGATGAAGAACTCTTTCCCCTTCAAGTAGTCA
  TGAAGAAGCTGGATCTTATCAGCGAGCTTAGAGAGCGAGTCCAAGCACAGTGTGACTGCTGTTTTTTATA
  CTGGCATCGCGCTGTCTTCCCGATTTATTTAGATGATGTATATGAAAATGCTGTCGATGCAGCCAGATTA
  CATTACATGTTCAGTGCTTTACGAGACTGTGTGCCTGCCATGATGCATTCAAGGCATTTAGAGTCCCATG
  AGCTGCTTTTGGATTGCTATGACAAGGAAATCATGGACATTTTAAATGAGCATTTGCTGGATAAGCTGTG
  TAAGGAGATCGAGAAGGACTTGCGGCTTTCTGTGCATACTCACTTGAAGCTGGATGACCGGAACCCCTTC
  AAAGTTGGCAGGAAAGACTTGGCGCTCTTTTTCTCTCTGAACCCTATTCGGTTTTTCAATCGATTCATTG
  ATATTCGAGCTTATGTAACTCACTATCTGGATAAGACTTTCTACAATCTCACAACAGTAGCTCTTCATGA
  TTGGGCCACTTACAGTGAAATGAGAAATCTGGCTACGCAGCGTTATGGTTTGGTTATGACAGAGGCACAT
  CTTCCTAGCCAGACTTTGGAGCAGGGACTCGATGTCCTGGAAATTATGAGAAACATTCACATATTTGTCT
  CAAGATACCTCTATAATCTCAATAACCAGATTTTTATTGAACGGACAAGCAACAATAAACATTTGAATAC
  TATTAATATTCGACACATTGCTAATTCGATTCGAACACATGGGACGGGGATCATGAATACAACAGTTAAT
  TTCACGTACCAGTTTTTGAAAAAGAAGTTCTATATATTTAGTCAGTTTATGTATGATGAGCATATCAAAT
  CCAGATTGATTAAAGATATTCGGTTTTTCAGGGAGATCAAGGACCAAAATGACCACAAGTATCCTTTTGA
  TAGAGCAGAAAAATTCAATCGTGGCATAAGAAAACTTGGAATCACACCAGAGGGACAGAGCTACCTTGAC
  CAGTTCAGGCAGCTCATCAGCCAGATTGGTAATGCTATGGGCTATATACGCATGATAAGATCTGGTGGTC
  TTCATTGTAGCAGCAATGCCATTAGATTCGTGCCGGATCTTGAAGACATTGTGAGTTTTGAAGAGCTCGT
  GAAAGAAGAAGGGCTTGCAGAAGAAACACTTAGAGCAGCCAGGCATTTGGACTCAGTCCTCAGTGATCAT
  ACACGGAACTCTGCTGAAGGTACAGAATATTTCAAAATGCTTGTAGATGTTTTTGCTCCAGAATTTCGAA
  GGCCAAAGAATATACATCTCCGAAATTTCTATATCATTGTTCCTCCTCTTACTCTGAACTTTGTAGAACA
  TTCTATAAGTTGCAAAGAGAAGTTGAATAAAAAAAATAAACTAGGAGCTGCATTTACTGACGATGGTTTT
  GCCATGGGTGTGGCTTATACCTTAAAGCTTTTGGATCAGTACCAAGAGTTTGATTCACTTCATTGGTTCC
  AGTCTGTTAGAGAAAAATACATAAAGGAGATCAGAGCAGTTGCTAAGCAACAGAACGTGCAGTCAACTAG
  TCAAGATGAAAAACTACTCCAAACCATGAACCTTACTCAGAAGCGACTGGAGGTCTATTTACAGGAATTT
  GAGCTGCTGTATTTCTCCTTGAGCAGTGCAAGAATATTCTTCAGAGCAGACAAGACCGCAGCTGAAGAGA
  ACCAAGAAAAGAAAGAGAAGGAAGAAGAAACTAAAACAAGCAATGGAGATGGACCCGAGAGCACTGTGTC
  TGCAGATCCCGTGGTGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033375
• Insert Size: 3522 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033375.2, NP_001028547.2
• RefSeq Size: 5863 bp
• RefSeq ORF: 3522 bp
• Locus ID: 319277
• UniProt ID: Q3UMB9
• Cytogenetics: 10 C1
• Gene Summary: Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875).[UniProtKB/Swiss-Prot Function]