Asap1 (NM_010026) Mouse Untagged Clone

CAT#: MC223725

Asap1 (untagged) - Mouse ArfGAP with SH# domain, ankyrin repeat and PH domain1 (Asap1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asap1
• Synonyms: AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223725 representing NM_010026
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATCTTCAGCCTCCCGGCTCTCCAGTTTTTCCTCCAGAGATTCACTATGGAATCGGATGCCGGACC
  AGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC
  CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC
  CTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAAAATGAAGAAA
  ACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTTGGCACCGC
  TTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCAGGGTCTGAGC
  CACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGAGATCTCAAGA
  AGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGAAAAGAGAGCA
  CGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAATGGAAAAGGAA
  CGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACCAAAAAGGGTG
  TGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATGGCTTGAAAAC
  GGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGAT
  GAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGATCCGAAAGAAG
  TAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTTCTAGAAGAGACTCTCAGAGCCGGCAAGGTGGGTA
  CAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGT
  GATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACG
  CAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAAGTGAAACCGAATGCTGAGGACAA
  GAAGTCTTTTGACCTGATATCACATAATCGAACATATCACTTTCAAGCAGAAGATGAGCAGGATTATATA
  GCGTGGATATCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCA
  CAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCCATCATCGAGGATGTACAGCGGCTCCCTGGGAATGA
  CATCTGCTGTGACTGCGGCTCATCAGAACCCACGTGGCTTTCAACCAACTTGGGTATTTTGACCTGTATA
  GAATGTTCCGGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAAT
  TAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAAA
  TTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACCGTGCGTAAAGAGTACATCACTGCA
  AAGTATGTCGACCACCGCTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTG
  AGGCCATCAAGTCCAGGGACTTACTTGCATTAATCCAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCC
  ACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAGCCCTTCATCTTGCCGTCAGGACCGCAGACCAGACA
  TCTCTCCATCTGGTTGATTTCCTTGTACAAAACTGTGGGAACCTGGATAAGCAAACGTCTGTGGGGAATA
  CAGTTCTGCACTATTGCAGCATGTACGGCAAGCCGGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCAC
  CGTGGACATCGTTAACCAGAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACCCAGTGT
  GAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAACCCTCATGTCCACGTAGAGTATGAGTGGAATC
  TTCGACAGGACGAGATGGATGAGAGCGATGACGATCTGGATGACAAGCCAAGCCCGATCAAAAAGGAACG
  CTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCAGCATCTCGCCACAGGACAAGCTGGCACTGCCAGGG
  TTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGCCTCTACGA
  GCACAGATTTGCCCACATCACCCACCAGTGAGGCTCCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTCC
  AACTGGCCCACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAG
  AGGCCTCCTCCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCACGGGC
  CCCCAAACAAAGGCGCAATTCCTTGGGGTAATGATGTGGGCCCATTATCTTCAAGTAAGACGGCCAACAA
  GTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCT
  AAACTACCTCAGAAAGTGGCACTAAGGAAGACGGAGACCAGCCATCATCTCTCCCTCGACAGAACCAACA
  TCCCACCTGAGACTTTTCAGAAATCATCACAGTTGACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCT
  GCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCCAAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTA
  CCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAGA
  TGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGATCTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGC
  CAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGTCACACACCGGGGATCTGTCTCCAAATGTACAGTCC
  AGAGATGCCATCCAGAAGCAAGCATCTGAAGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCCG
  TACCACTGCCGAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGGGTGAAGACCATTTATGACTGCCA
  GGCAGATAATGACGACGAACTCACATTTATTGAGGGGGAAGTGATCATTGTCACCGGGGAAGAGGACCAG
  GAGTGGTGGATCGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACA
  TCCTGTCTGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010026
• Insert Size: 3444 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010026.3, NP_034156.2
• RefSeq Size: 6281 bp
• RefSeq ORF: 3444 bp
• Locus ID: 13196
• UniProt ID: Q9QWY8
• Cytogenetics: 15 D1
• Gene Summary: May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).