Amer1 (NM_175179) Mouse Untagged Clone

CAT#: MC223690

Amer1 (untagged) - Mouse family with sequence similarity 123, member B (Fam123b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amer1
• Synonyms: 2810002O09Rik; AW492303; Fam123b; Wtx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223690 representing NM_175179
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTCAACAGGATGAAGCTGTTCAAACCAAAGGAGCATCAACTTCTAGCGATGCCCAAGACCAGG
  GAGCTGAAAAGGGAGCCAAGAATAAGACAACTGAGGCAACAGAAGGACCAACATCAGAGCCTCCCTTATC
  AGGCCCAGGTAGGCTGAAGAAAACTGCCATGAAACTCTTTGGTGGCAAGAAGGGAATCTGTACTCTGCCT
  AGTTTCTTTGGGGGTGGACGAAGCAAAGGTTCTGGGAAAGTCAGCTCTAAGAAAAGTCTAAACAAGAGTA
  AGACTCACGATGGTCTAAGTGAAGCGTCTCAGGGACCTGAAGATGTTGTCATTGAAGAAACAGACCTCTC
  TACACCTTTGTCCAAGTCATCTGCTCAATTTCCTAGCTCCCAGAGTGCCAATGGAGCGTTGGAGATAGGC
  TCTAAACATAAGACATCTGGGACTGAAGCCATAGAAAAAGCTGGAGTTGAGAAGGTACCCTCTGTGCACA
  AGCCGAAGAAAAGCCTAAAAAGCTTCTTTAGCAGTATCCGCCGTCACCGGAAGGGCAAGACTTCTGGGGC
  TGACCAAAGTGTGCCAGGGGCCAAGGAACTTGAGGGGGCAAGAACCAGGTCTCATGAGCATGTGAGTTCA
  ATCTCTCTGCCCAGCTCTGAGGAGATATTCCGAGACACTAGAAAGGAAAATGCCAAACCCCAGGATGCCC
  CTGGACCAAAAATGTCTCCAGCACAAGTACATTTTTCACCAACTACTGAGAAGGCAGCTTGTAAAAATCC
  AGAAAAACTCACAAGGACCTGTGCCTCAGAGTTCATGCAGCCCAAGCCTGTTCTTGAAGGTGGTAGTCTA
  GAGGAGCCCCACACTTCAGAAACAGAAGGAAAAGTGGTGGCAGGAGAAGTAAACCCACCCAATGGCCCTG
  TTGGGGATCAGCTAAGCCTCTTGTTTGGGGATGTTACATCGCTAAAAAGCTTTGACTCACTGACAGGTTG
  TGGTGACATTATAGCAGAACAGGACATGGACAGCATGACAGATAGCATGGCCTCTGGTGGCCAGAGGGCC
  AACCGAGATGGGACCAAGCGAAGTTCCTGCTTGGTAACCTACCAAGGAGGTGGGGAGGAGATGGCCTTGC
  CAGATGATGATGACAATGATGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA
  AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATTATTAGAGGATGAAGAGGAGGTTAAGGATGGA
  GAGGAAAATGATGACTTAGAGTATCTGTGGGCAAGTGCCCAGATATACCCAAGATTTAATATGAATCTGG
  GCTACCATACTGCTATATCTCCCAGCCACCAGGGCTACATGCTTCTTGACCCAGTTCAGTCTTATCCTAA
  TCTAGGCCTTGGAGAACTTTTGACACCTCAGAGCGACCAGCAAGAGTCTGCTCCCAATAGTGATGAAGGT
  TATTATGACTCTACCACACCAGGATTTGAGGATGATTCAGGTGAGGCTCTGGGACTTGCCCACAGGGATT
  GTCTACCCAGGGACAGCTATAGTGGTGATGCGCTATATGAGTTCTATGAGCCAGATGACAGTCTTGAGCA
  CTCCCCACCTGGGGATGACTGCCTATATGACCTCCGTGGACGCAACTCAGAGATGTTGGATCCTTTCTTG
  AACTTGGAGCCCTTTTCTTCTCGGCCACCTGGGGCAATGGAAACCGAGGAAGAACGGCTAGTGACCATCC
  AGAAGCAGTTGCTATACTGGGAACTTCGCCGGGAGCAGCGTGAGGCTCAAGAGGCATGTGCCAGAGAGGC
  TCATGCCAGAGAAGCTTATGCCCGAGACACCCATACTCGGGAATCTTATGGCAGAAATGTCCGAGCCCGA
  GAAACTCAGGCCTTAGAGGCTCATTCTCAAGAGGGTCGAGTCCAAGAGACCAAGGTACGGCAGGAGAAGC
  CTGCCTTGGAGTATCAGATGAGGCCCTTAGGTCCCTCAGTGATGGGTCTGGTGGCAGGGACATCAGGAGG
  TTCTCAGACTTCTCACCGGGGAACCACTTCAGCTTTCCCTGCTACTTCTAGCAGTGAGCCAGACTGGAGG
  GATTTCCGTCCTCTGGAGAAGCGTTTTGAGGGGACTTGTTCCAAAAAAGATCAAAGTACTTGTTTAATGC
  AGCTCTTCCAGAGTGATGCCATGTTTGAGCCAGACATGCAAGAAGCAAATTTTGGAGGCTCACCCAGGAA
  GGCGTACCCTAGTTACTCACCCCCTGAGGAGCCAGAGGAAGAAGAAGAGGAGAAGGAAGGGAATGCCACT
  GTGAGTTTCTCACAGGCCCTAGTAGAGTTCACCAGCAATGGAAACCTTTTTACCAGCATGTCCTACAGCT
  CTGATTCTGACTCATCCTTCACTCAAAACCTCCCTGAGCTGCCCCCCATGGTCACCTTTGATATTGCTGA
  CGTGGAACGAGATGGAGAAGGCAAGTGTGAAGAGAATCCTGAGTTCAACAATGACGAAGATCTTACAGCC
  TCCTTGGAAGCTTTTGAGCTGGGTTATTACCACAAACATGCCTTCAACAGCTATCACAGTCGGTTCTACC
  AAGGCCTGCCCTGGGGAGTTAGCAGCCTCCCTCGATACTTAGGACTCCCCGGAGTCCACCCTCGACCTCC
  TCCTGCTGCAATGGCACTCAATAGGAGAAGCCGTTCCCTTGACAATGCAGAGTCTCTGGAGCTGGAGCTC
  TCCAGTTCTCACCTAGCCCAGGGTTACATGGAGTCTGATGAGCTCCAGGCCCACCAGGAAGATTCAGATG
  AAGAAGGTGAAGAAGAGGAAGGAGAATGGGGTCGGGATAGTCCCTTGTCTCTCTACACTGAACCTCCAGG
  GGTCTATGACTGGCCTCCCTGGGCTCACTGTCCTCTTCCAGTGGGGCCAGGTCTTGCTTGGATGAGCCCT
  AATCAGTTGTATGAGCCTTTCAATCAGTCTTCCTATGTGCAGGCAACCTGTTGTGTACCTCCTGTGGCAA
  TGCCAGTGTCAGTGCCAGGAAGAACACCTGGAGACTCTGTGTCTCAGCTAGCTCGACCTTCGCACCTACC
  CCTGCCCATGGGCCCTTGCTATAACCTTCAATCACAGGCCTCCCAGAGTGGGAGGGCCAAGCCTCGGGAT
  GTACTGCTGCCTGTAGATGAGCCCAGTTGCTCTTCCATTTCTGGAGCCAACTCTCAGTCTCAAGCCAAGC
  CTGTAGGTATCACCCATGGTATCCCTCAGCTGCCCAGGGTGCGGCCTGAACCCTTCCAGCTTCAACCCAA
  TCACTATCGAGCTTCCAACCTTGACCTGTCAAAGGAGAGGGGTGAGCAAGGTGCCTCTCTCTCTACCAGC
  TACTCCTCTACTGCCATGAATGGAAACCTAGCCAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175179
• Insert Size: 3399 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175179.4, NP_780388.2
• RefSeq Size: 8476 bp
• RefSeq ORF: 3399 bp
• Locus ID: 72345
• UniProt ID: Q7TS75
• Cytogenetics: X C3
• Gene Summary: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development (By similarity).[UniProtKB/Swiss-Prot Function]