Inpp5d (NM_001110193) Mouse Untagged Clone

CAT#: MC223682

Inpp5d (untagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Inpp5d
• Synonyms: p150Ship; s-SHIP; SHIP; SHIP-1; SHIP1; SIP-145
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223682 representing NM_001110193
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCCATGGTCCCTGGGTGGAACCATGGCAACATCACCCGCTCCAAGGCAGAGGAGCTACTTTCCA
  GAGCCGGCAAGGACGGGAGCTTCCTTGTGCGTGCCAGCGAGTCCATCCCCCGGGCCTACGCACTCTGCGT
  GCTGTTCCGGAATTGTGTTTACACTTACAGGATTCTGCCCAATGAGGACGATAAATTCACTGTTCAGGCA
  TCCGAAGGTGTCCCCATGAGGTTCTTCACGAAGCTGGACCAGCTCATCGACTTTTACAAGAAGGAAAACA
  TGGGGCTGGTGACCCACCTGCAGTACCCCGTGCCCCTGGAGGAGGAGGATGCTATTGATGAGGCTGAGGA
  GGACACTGTAGAAAGTGTCATGTCACCACCTGAGCTGCCTCCCAGAAACATTCCTATGTCTGCCGGGCCC
  AGCGAGGCCAAGGACCTTCCTCTTGCAACAGAGAACCCCCGAGCCCCTGAGGTCACCCGGCTGAGTCTCT
  CCGAGACACTGTTTCAGCGTCTACAGAGCATGGATACCAGTGGGCTTCCCGAGGAGCACCTGAAAGCCAT
  CCAGGATTATCTGAGCACTCAGCTCCTCCTGGATTCCGACTTTTTGAAGACGGGCTCCAGCAACCTCCCT
  CACCTGAAGAAGCTGATGTCACTGCTCTGCAAGGAGCTCCATGGGGAAGTCATCAGGACTCTGCCATCCC
  TGGAGTCTCTGCAGAGGTTGTTTGACCAACAGCTCTCCCCAGGCCTTCGCCCACGACCTCAGGTGCCCGG
  AGAGGCCAGTCCCATCACCATGGTTGCCAAACTCAGCCAATTGACAAGTCTGCTGTCTTCCATTGAAGAT
  AAGGTCAAGTCCTTGCTGCACGAGGGCTCAGAATCTACCAACAGGCGTTCCCTTATCCCTCCGGTCACCT
  TTGAGGTGAAGTCAGAGTCCCTGGGCATTCCTCAGAAAATGCATCTCAAAGTGGACGTTGAGTCTGGGAA
  ACTGATCGTTAAGAAGTCCAAGGATGGTTCTGAGGACAAGTTCTACAGCCACAAAAAAATCCTGCAGCTC
  ATTAAGTCCCAGAAGTTTCTAAACAAGTTGGTGATTTTGGTGGAGACGGAGAAGGAGAAAATCCTGAGGA
  AGGAATATGTTTTTGCTGACTCTAAGAAAAGAGAAGGCTTCTGTCAACTCCTGCAGCAGATGAAGAACAA
  GCATTCGGAGCAGCCAGAGCCTGACATGATCACCATCTTCATTGGCACTTGGAACATGGGTAATGCACCC
  CCTCCCAAGAAGATCACGTCCTGGTTTCTCTCCAAGGGGCAGGGAAAGACACGGGACGACTCTGCTGACT
  ACATCCCCCATGACATCTATGTGATTGGCACCCAGGAGGATCCCCTTGGAGAGAAGGAGTGGCTGGAGCT
  ACTCAGGCACTCCCTGCAAGAAGTCACCAGCATGACATTTAAAACAGTTGCCATCCACACCCTCTGGAAC
  ATTCGCATAGTGGTGCTTGCCAAGCCAGAGCATGAGAATCGGATCAGCCATATCTGCACTGACAACGTGA
  AGACAGGCATCGCCAACACCCTGGGAAACAAGGGAGCAGTGGGAGTGTCCTTCATGTTCAATGGAACCTC
  CTTGGGGTTCGTCAACAGCCACTTGACTTCTGGAAGTGAAAAAAAGCTCAGGAGAAATCAAAACTATATG
  AACATCCTGCGGTTCCTGGCCCTGGGAGACAAGAAGCTAAGCCCATTTAACATCACCCACCGCTTCACCC
  ACCTCTTCTGGCTTGGGGATCTCAACTACCGCGTGGAGCTGCCCACTTGGGAGGCAGAGGCCATCATCCA
  GAAGATCAAGCAACAGCAGTATTCAGACCTTCTGGCCCACGACCAACTGCTCCTGGAGAGGAAGGACCAG
  AAGGTCTTCCTGCACTTTGAGGAGGAAGAGATCACCTTCGCCCCCACCTATCGATTTGAAAGACTGACCC
  GGGACAAGTATGCATACACGAAGCAGAAAGCAACAGGGATGAAGTACAACTTGCCGTCCTGGTGCGACCG
  AGTCCTCTGGAAGTCTTACCCGCTGGTGCATGTGGTCTGTCAGTCCTATGGCAGTACCAGTGACATCATG
  ACGAGTGACCACAGCCCTGTCTTTGCCACGTTTGAAGCAGGAGTCACATCTCAATTCGTCTCCAAGAATG
  GTCCTGGCACTGTAGATAGCCAAGGGCAGATCGAGTTTCTTGCATGCTACGCCACACTGAAGACCAAGTC
  CCAGACTAAGTTCTACTTGGAGTTCCACTCAAGCTGCTTAGAGAGTTTTGTCAAGAGTCAGGAAGGAGAG
  AATGAAGAGGGAAGTGAAGGAGAGCTGGTGGTACGGTTTGGAGAGACTCTTCCCAAGCTAAAGCCCATTA
  TCTCTGACCCCGAGTACTTACTGGACCAGCATATCCTGATCAGCATTAAATCCTCTGACAGTGACGAGTC
  CTATGGTGAAGGCTGCATTGCCCTTCGCTTGGAGACCACAGAGGCTCAGCATCCTATCTACACGCCTCTC
  ACCCACCATGGGGAGATGACTGGCCACTTCAGGGGAGAGATTAAGCTGCAGACCTCCCAGGGCAAGATGA
  GGGAGAAGCTCTATGACTTTGTGAAGACAGAGCGGGATGAATCCAGTGGAATGAAATGCTTGAAGAACCT
  CACCAGCCATGACCCTATGAGGCAATGGGAGCCTTCTGGCAGGGTCCCTGCATGTGGTGTCTCCAGCCTC
  AATGAGATGATCAATCCAAACTACATTGCCAACCGAGGTCCCTGCCCCAGGGTGCAAGAGGCAAGACCTG
  GGGATCTGGGAAAGGTGGAAGCTCTGCTCCAGGAGGACCTGCTGCTGACGAAGCCCGAGATGTTTGAGAA
  CCCACTGTATGGATCCGTGAGTTCCTTCCCTAAGCTGGTGCCCAGGAAAGAGCAGGAGTCTCCCAAGATG
  CTGCGGAAGGAGCCCCCGCCCTGTCCAGACCCAGGAATCTCATCACCCAGCATCGTGCTCCCCAAAGCCC
  AAGAGGTGGAGAGTGTCAAGGGGACAAGCAAACAGGCCCCTGTGCCTGTCCTTGGCCCCACACCCCGGAT
  CCGCTCCTTTACCTGTTCTTCTTCTGCTGAGGGCAGAATGACCAGTGGGGACAAGAGCCAAGGGAAGCCC
  AAGGCCTCAGCCAGTTCCCAAGCCCCAGTGCCAGTCAAGAGGCCTGTCAAGCCTTCCAGGTCAGAAATGA
  GCCAGCAGACAACACCCATCCCAGCTCCACGGCCACCCCTGCCAGTCAAGAGTCCTGCTGTCCTGCAGCT
  GCAACATTCCAAAGGCAGAGACTACCGTGACAACACAGAACTCCCCCACCATGGCAAGCACCGCCAAGAG
  GAGGGGCTGCTTGGCAGGACTGCCATGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110193
• Insert Size: 3393 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110193.2, NP_001103663.1
• RefSeq Size: 4757 bp
• RefSeq ORF: 3393 bp
• Locus ID: 16331
• UniProt ID: Q9ES52
• Cytogenetics: 1 44.44 cM
• Gene Summary: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (By similarity). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9367159). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains two alternate in-frame exons in the 3' coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.