Dgkz (NM_001166597) Mouse Untagged Clone

CAT#: MC223658

Dgkz (untagged) - Mouse diacylglycerol kinase zeta (Dgkz), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgkz
• Synonyms: E130307B02Rik; ENSMUSG00000075035; F730209L11Rik; mDGK[z]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223658 representing NM_001166597
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCTTCTTTAGGAAGCATTTCCAACGAAAGGCACGAGGTCCCGGAGAGAGGCAGCGGCGGTCCA
  GTGGCGTGGGGCTGCCCACAGGCAAGGCTCGACGCCGCTCCCCTGCAGGGCAGGCGTCCTCTTCACTGGC
  CCAGAGGCGCCGCTCCAGTGCACAGCTCCAGGGCTGTTTTCCCAGCTGCGGGGTGGGCACGAGAGGTGCC
  AGCCGCAGGCGATCCAGCACTGCACCCCCTACCTGCAATCCCCGCTTCATGGTGGATGTGGTGCCTACCC
  TACAGCCTGTCACAGTTGGGGCCCAGCTTCTGGGTGCACCCCTGCTGTTGGCTGGGCTCACAAGCATGAA
  GGAGGAAGAGGAGGAGGAAGGGGTCCAGGAGGGAAATGAGACAGCTGAGGCGACAAGTGATGCTAGGCCA
  GGTACCCGGACAACGCCCTTCTCACACCAGGGCACCTGGCCACTGCTTCCCATGCCCCGCTGCCTGCGTC
  GAGCCTCCTCCCACCTGCTCCCTGCTGATGTGGAGTGTGGCCATACCCTTTGGGGCCTGCATGGTCACTA
  TCGACGCCTCAGCCAGCGGTGGCCCTCAGGCCAGCACCCTGGTCTTGGGGGCCGAAGCCCAAGAGCCTCG
  GGCGCTGCAGCATGCCCCATGATACCGGCCCGTGTGCGCCCACTGTCTCGCAGACGCCAGGTAGCTCTAA
  GGCGGAAGTCAGCTGGACCCCAGCCCTGGAGCACCCTGCTTATGAAAGCCATCACCAAGTCGGGCCTCCA
  GCACCTGGCACCCCCTCCTCCCACGCCCGGGGCCCCGTGCGGTGAATCTGAGGAGCAGATCCAGAGCACT
  GTGGACTGGAGTGAGTCAGCAGTGTATGGGGAGCACATCTGGTTTGAGACCAACGTGTCCGGTGACTTCT
  GCTATGTCGGGGAGCAGCACTGTGTAGCTAAGATGCTGCCGAAGTCAGCGCCCAGAAAAAAGTGTGCAGC
  CTGTAAGATCGTGGTGCATACCCAATGCATTAAGCAGCTGGAAAAGATCAATTTCCGCTGTAAGCCGTCC
  TTCCGCGAATCAGGCTCCAGGAATGTCCGTGAGCCAACCTTCGTAAGACACCACTGGGTCCACAGACGAC
  GCCAGGATGGCAAGTGTCGGCACTGTGGGAAGGGCTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGAT
  CGTAGCCATCAGCTGCTCCTGGTGCAAACAGGCATACCACAGCAAGGTGTCGTGCTTCATGATGCAACAG
  ATTGAGGAACCCTGCTCCCTAGGGGTGCATGCAGCCGTGGTCATCCCACCCACCTGGATCCTGCGGGCCC
  GGAGGCCCCAGAACACCCTCAAGGCCAGCAAGAAGAAAAAGAGAGCGTCCTTCAAGAGGAGGTCCAGCAA
  GAAAGGACCTGAGGAAGGCCGCTGGAGACCCTTCATCATCAGACCCACCCCATCCCCCCTCATGAAACCC
  CTGCTGGTGTTTGTGAACCCCAAGAGTGGGGGCAACCAGGGTGCCAAGATCATCCAGTCTTTTTTGTGGT
  ATCTGAATCCCCGACAAGTCTTTGATCTGAGCCAGGGAGGACCCAGGGAAGCGCTGGAAATGTACCGCAA
  AGTACATAATTTGAGGATTCTGGCTTGCGGGGGTGATGGCACGGTTGGCTGGATTCTCTCCACCCTGGAC
  CAGCTGCGCTTAAAACCACCGCCTCCTGTAGCCATCCTGCCTCTGGGCACTGGCAATGACCTGGCCCGAA
  CCCTCAACTGGGGTGGGGGTTACACAGATGAGCCTGTGTCAAAGATCCTTTCCCATGTTGAGGAGGGGAA
  TGTGGTACAGCTGGACCGTTGGGACCTCCGAGCAGAGCCCAACCCTGAGGCGGGGCCTGAGGAGCGAGAT
  GATGGAGCCACTGACCGGCTGCCCCTGGATGTCTTTAACAACTACTTCAGCCTGGGCTTCGATGCCCACG
  TCACCCTGGAATTTCATGAGTCTCGAGAAGCCAACCCAGAGAAGTTCAACAGCCGCTTTCGGAATAAGAT
  GTTCTATGCTGGGACGGCCTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACTTAGCCAAGCACATCCGA
  GTAGTGTGTGACGGAATGGACCTAACCCCCAAGATTCAGGACCTGAAACCGCAGTGCATCGTCTTTCTGA
  ATATCCCCAGGTACTGTGCAGGCACCATGCCCTGGGGCCACCCCGGGGAGCACCATGACTTCGAGCCCCA
  GAGGCATGATGATGGCTACCTGGAGGTCATCGGCTTCACCATGACATCCTTGGCAGCACTGCAGGTGGGT
  GGGCACGGCGAGCGATTGACGCAGTGCCGAGAAGTGCTGCTCACCACTGCCAAGGCCATCCCTGTGCAGG
  TGGACGGTGAGCCCTGCAAGCTTTCAGCATCACGGATTCGAATTGCCCTGCGCAACCAGGCCACTATGGT
  GCAGAAGGCCAAGCGCCGGAGTACTGCCCCACTGCACAGCGACCAGCAGCCAGTTCCTGAGCAGCTGAGG
  ATCCAGGTGAGCAGGGTCAGTATGCACGACTACGAGGCTCTACATTATGACAAGGAGCAGCTCAAGGAAG
  CCTCTGTGCCTCTGGGCACTGTGGTGGTCCCCGGAGACAGTGACCTGGAGCTCTGCCGCGCCCACATTGA
  GAGACTGCAGCGGGAGCCCGATGGGGCTGGAGCCAAGTCCCCGATGTGCCACCAACTCTCCTCCAAGTGG
  TGTTTCCTGGATGCCACCACTGCCAGCCGCTTCTACAGGATCGACAGAGCCCAGGAGCACCTCAACTATG
  TGACGGAGATTGCCCAGGACGAGATTTACATCCTAGACCCAGAGCTTCTGGGAGCATCAGCCCGGCCTGA
  CCTCCCCACCCCTACCTCCCCACTCCCTGCTTCCCCCTGCTCCCCTACACCCGGGTCAATGCAGGGGGAC
  ACTGCACTGCCCCAAGGTGAAGAACTGATTGAAGCAGCCAAGAGGAATGACTGCTGCAAGCTCCAGGAGC
  TACACCGAGCAGGAGGTGACCTCATGCACCGTGACCAGAAGAGCCGCACGCTCTTGCACCACGCGGTCAG
  CACTGGCAGTAAGGAAGTGGTCCGCTATCTGCTGGATCATGCACCTCCAGAGATCCTTGATGCTGTGGAG
  GAGAATGGGGAGACCTGTCTACACCAGGCAGCCGCCCTGGGTCAGCGCACCATCTGCCACTACATTGTGG
  AAGCCGGGGCCTCCCTCATGAAGACAGATCTGCAGGGCGACACTCCCCGGCAGCGCGCTGAGAAGGCTCA
  AGACACAGAGCTCGCTGCCTACCTGGAGAACAGACAGCATTACCAGATGATCCAGCGTGAGGACCAGGAG
  ACAGCTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166597
• Insert Size: 3372 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166597.1, NP_001160069.1
• RefSeq Size: 4090 bp
• RefSeq ORF: 3372 bp
• Locus ID: 104418
• Cytogenetics: 2 E1
• Gene Summary: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Regulates RASGRP1 activity (By similarity). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Activates PIP5K1A activity via generation of phosphatidic acid (PubMed:27739494).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).