Gucy2d (NM_001130693) Mouse Untagged Clone

CAT#: MC223612

Gucy2d (untagged) - Mouse guanylate cyclase 2d (Gucy2d), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gucy2d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223612 representing NM_001130693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTCTGCAGCAGGGCTGTCACTTTGAAGGCCAGAACTGGACAGCACCACACTGGAAGACCTGCC
  TGCCCTGCCAGGGCCCGTGGAGACTCACTGTCAGTCATCTGAAGACTGTCTCTTCCATATCAGTCCTCTC
  TGTGGTATTTTGGAGTGTCCTACTTTGGGCTGACTCCCTTTCCCTGTTAGCATGGGCAAGAGAGACCTTC
  ACCCTTGGGGTGCTGGGTCCCTGGGACTGTGACCCCATCTTTGCCCAGGCCCTCCCCAGCATAGCTACCC
  AGCTGGCTGTAGATCAAGTTAATCAGGATGCCTCACTGCTGCCAGGCTCACAGTTGGATTTCAAGGTCCT
  CCCCACAGGCTGTGACACTCCTCATGCCCTGGCCACATTCGTGGCCCACAAGAACATAGTGGCTGCTTTT
  GTAGGCCCTGTTAATCCTGGGTTCTGCTCAGCAGCAGCCCTGCTGGCCCAAGGCTGGGGCAAGTCCCTCT
  TCTCCTGGGCCTGTGAAGCTCCAGAGGGAGGAGGTGACTTAGTGCCCACTTTACCTTCTGCGGCCGATGT
  GCTACTGTCTGTCATGAGACACTTTGGCTGGGCTCGCTGGGCCATTGTGTCCTCTCACCAGGACATCTGG
  GTAACCACAGCCCAGCAGCTGGCCACTGCTTTCAGGACTCATGGGCTGCCCATCGGACTGGTGACCTCTT
  TGGGACCTGGAGAGAAGGGGGCCACAGAGGTTTGCAAGCAGCTCCACAGTGTTCATGGTCTGAAAATTGT
  GGTTCTGTGCATGCACTCGGCGCTGCTTGGAGGCCTGGAGCAGACCACCCTGCTGCACTGTGCCTGGGAG
  GAGGGCCTCACAGACGGAAGGCTGGTCTTCCTGCCCTACGACACGCTGCTCTTTGCCCTGCCCTATGGAA
  ACCGCTCCTACCTGGTCCTGGATGACCATGGGCCCCTTCAGGAAGCCTATGACGCAGTACTCACTGTCAG
  TCTGGAGTCCAGTCCTGAGAGCCACGCCTTCACTGCTACCGAGATGAGTGGAGGGGCAACTGCCAACTTG
  GAGCCAGAGCAGGTGTCCCCACTCTTTGGAACTATCTATGATGCCGTCATCCTGTTGGCCCATGCCCTGA
  ACCGATCTGAGACCCATGGAGCGGGGCTCTCAGGGGCTCACTTAGGGGACCACGTAAGAGCTCTTGATGT
  GGCTGGTTTTAGCCAGAGAATCCGAACAGATGGGAAAGGCAGAAGGCTAGCCCAATATGTCATCCTGGAC
  ACAGATGGTGAAGGAAGCCAGTTGGTTCCTACCCACATTCTGGACACAAGCACATGGCAGGTGCAGCCTC
  TGGGAAAGCCTATACACTTTCCTGGGGGGAGCCCTCCAGCCCATGACGCCAGCTGCTGGTTCGACCCCAA
  CACACTATGCATCAGAGGTGTGCAGCCCCTGGGCAGCCTCCTGACTCTGACGATAGCCTGTGTCCTGGCA
  CTGGTTGGTGGATTCCTTGCTTACTTCATCAGGTTAGGCCTCCAGCAGCTGCGGCTGTTGCGGGGCCCCC
  ATCGGATCCTGCTGACATCTCAGGAGCTCACCTTCCTCCAGCGGACCCCCAGCCGGAGGAGGCCACATGT
  GGACAGCGGCAGTGAGTCAAGAAGTGTGGTGGATGGTGGAAGTCCACGGTCGGTGACCCAGGGGTCAGCA
  AGGAGCCTACCAGCCTTCCTGGAGCACACCAACGTGGCCCTGTACCAGGGAGAGTGGGTATGGCTGAAGA
  AGTTTGAAGCAGGCGTGGCTCCTGATCTTCGGCCAAGTAGCCTTAGCTTCCTGAGAAAGTTGCGGGAGAT
  GCGGCATGAGAATGTTACTGCCTTCCTGGGCCTCTTTGTGGGCCCTGGGGTCAGTGCAATGGTGCTGGAG
  CACTGTGCCCGTGGCAGCCTGGAGGACCTGCTGCAGAATGAGAACCTGAGGCTAGACTGGACCTTCAAGG
  CCTCCTTACTGTTGGATCTGATCCGAGGTTTGCGGTATCTGCACCATCGGCGCTTCCCCCACGGGCGCCT
  CAAGTCCAGGAACTGTGTGGTGGACACTCGCTTTGTGCTCAAGATCACTGATCATGGCTATGCAGAGTTC
  CTGGAGTCTCACTGTTCTTCCAGGCCCCAGCCAGCCCCAGAAGAGCTTCTGTGGACGGCTCCTGAGCTGC
  TGCGGGGGCCTGGGAAGGCCACCTTCAAAGGTGATGTTTTCAGCTTGGCCATCATCCTGCAGGAGGTACT
  GACCCGAGACCCACCCTACTGCTCCTGGGGACTCTCAGCAGAAGAGATCATCCGGAAGGTGGCATCTCCC
  CCTCCTCTGTGCCGGCCACTGGTGTCCCCTGACCAGGGTCCCCTCGAGTGTATCCAGTTGATGCAGCTAT
  GCTGGGAGGAAGCTCCAGATGACAGGCCAAGCTTGGACCAGATCTACACACAGTTCAAAAGCATCAACCA
  AGGCAAGAAGACAAGTGTTGTAGACTCCATGCTGCGGATGCTGGAGAAGTATTCCGAAAGCCTGGAAGAT
  CTGGTCCAGGAGCGGACTGAGGAGCTGGAACTGGAGAGGCGGAAGACAGAGAGGCTGCTCTCGCAGATGC
  TCCCTCCGTCTGTCGCCCATGCTCTAAAGATGGGGACAACAGTGGAGCCCGAGTACTTCGACCAGGTCAC
  CATCTATTTCAGTGACATCGTGGGCTTCACCACCATCTCAGCCTTGAGTGAGCCCATTGAGGTGGTGGGC
  TTCCTCAATGATCTCTACACGCTGTTTGATGCTGTTCTCGACAGCCATGATGTGTATAAGGTGGAGACCA
  TAGGGGATGCCTACATGGTGGCTTCTGGTCTCCCTCGGCGCAATGGAAATCGGCACGCTGCTGAGATCGC
  CAACCTGGCCCTGGACATCCTTAGCTATGCAGGCAACTTTCGGATGAGGCATGCACCCGATGTACCCATC
  CGTGTCAGGGCAGGTCTGCATTCAGGACCCTGTGTGGCAGGTGTTGTGGGTCTCACCATGCCTCGGTACT
  GCCTCTTTGGGGACACCGTCAACACTGCCTCTAGGATGGAGTCCACTGGGCTGCCTTACAGGATCCATGT
  CAGTCAAAGCACTGTCCAGGCCCTGCTCAGCCTTGATGAAGGCTACAAAATTGATGTCAGAGGTCAGACT
  GAACTGAAGGGGAAGGGCTTGGAGGAGACCTACTGGCTGACAGGGAAGGTAGGATTCTGCAGACCCCTGC
  CTACACCTCTGTCTATCAAGCCTGGAGACCCATGGCAGGACCGCATAAACCAAGAAATCCGGACTGGCTT
  TGCCAAAGCACGCCAGGGACTGGCTGAGCCCAGAAAATCAGGGGAGGCTGGGCCAGGTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001130693
• Insert Size: 3354 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130693.3, NP_001124165.1
• RefSeq Size: 3633 bp
• RefSeq ORF: 3354 bp
• Locus ID: 14918
• UniProt ID: A0A0U1RPR8
• Cytogenetics: 7 53.86 cM
• Gene Summary: Functions as an olfactory receptor activated by urine odorants, uroguanylin and guanylin and as well by the volatile semiochemicals carbon disulfide (CS2) and carbon dioxide (CO2) (PubMed:17724338, PubMed:17702944, PubMed:20637621). Has guanylate cyclase activity upon binding of the ligand (By similarity). Activation of GUCY2D neurons leads to the cGMP-dependent activation of the CNGA3 channels, membrane depolarization and an increase in action potential frequency (PubMed:17724338, PubMed:20637621). Signaling pathways activated by GUCY2D may trigger social behaviors such as acquisition of food preference (PubMed:20637621).[UniProtKB/Swiss-Prot Function]