Upf1 (NM_030680) Mouse Untagged Clone

CAT#: MC223607

Upf1 (untagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Upf1
• Synonyms: B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223607 representing NM_030680
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTGGAGGCGTACGGCCCCAGCTCGCAAACACTCACCTTCTTGGACACTGAGGAGGCCGAGCTGC
  TCGGCGCCGACACCCAGGGCTCCGAGTTCGAATTCACCGACTTCACCCTTCCCAGCCAGACGCAGACGCC
  CCCCGGCGGCCCCGGCGGCGCGGGAGGCCCGGGCGGAGCGGGCGCAGGCGGCGCGGCCGGCCAGCTCGAC
  GCACAAGTTGGACCAGAGGGCATCTTGCAAAATGGGGCTGTGGATGACAGTGTGGCCAAGACCAGCCAGC
  TGCTAGCTGAGCTGAACTTCGAGGAAGATGAAGAGGACACATACTACACTAAGGACCTCCCAGTCCACGC
  CTGCAGTTACTGTGGAATCCATGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGC
  AATGGCCGAGGAAATACTTCTGGCAGCCACATTGTGAATCACCTCGTGAGGGCAAAATGCAAGGAAGTGA
  CGCTGCACAAGGACGGGCCTCTGGGCGAGACCGTGCTGGAGTGCTACAACTGTGGCTGCCGCAACGTCTT
  CCTGCTGGGCTTCATCCCTGCGAAGGCCGACTCTGTGGTGGTGCTGTTGTGCAGGCAGCCCTGTGCCAGC
  CAGAGCAGCCTGAAGGACATCAACTGGGACAGCTCACAGTGGCAGCCCCTAATCCAGGACCGGTGCTTTC
  TGTCATGGCTGGTCAAGATTCCCTCTGAGCAGGAGCAGCTGCGAGCACGGCAGATCACGGCACAGCAGAT
  CAACAAGCTGGAAGAGCTCTGGAAGGAAAATCCTTCAGCCACTCTGGAGGACCTGGAGAAGCCAGGCGTA
  GACGAGGAGCCACAGCACGTGCTCCTGCGTTACGAGGATGCTTACCAGTACCAGAACATCTTCGGGCCAC
  TGGTCAAGCTGGAGGCTGACTATGACAAGAAGTTGAAGGAGTCACAGACTCAAGATAACATCACGGTCAG
  GTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTTCTTCACTTTGCCCAAGACTGACTCTGACATGCGG
  CTCATGCAGGGTGATGAGATCTGTCTGCGGTACAAAGGGGATCTGGCGCCCCTGTGGAAGGGGATTGGCC
  ACGTCATCAAGGTTCCTGATAATTATGGTGATGAGATTGCTATTGAGCTCCGCAGCAGCGTGGGTGCCCC
  TGTGGAAGTGACCCACAACTTCCAAGTGGATTTTGTGTGGAAGTCAACCTCTTTTGATAGGATGCAGAGT
  GCACTGAAGACCTTCGCTGTGGACGAGACCTCTGTGTCAGGGTATATTTACCACAAGCTGCTGGGCCACG
  AGGTGGAGGATGTGGTCATCAAGTGCCAGCTGCCAAAGCGCTTCACAGCTCAGGGGCTCCCTGACCTCAA
  CCACTCTCAGGTGTATGCTGTGAAGACCGTGCTGCAGAGACCACTCAGCCTCATCCAGGGCCCTCCAGGC
  ACAGGCAAGACTGTGACATCAGCCACTATTGTCTACCACCTTGCTCGGCAGGGCAATGGGCCTGTACTGG
  TTTGTGCTCCAAGTAACATCGCTGTGGACCAGCTCACAGAGAAGATCCACCAGACAGGACTGAAGGTCGT
  ACGCCTCTGTGCCAAGAGCCGTGAGGCCATTGACTCCCCAGTGTCCTTCCTGGCTTTGCACAACCAGATC
  AGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGCAGCAGCTAAAGGATGAGACAGGCGAGCTGTCAT
  CTGCAGATGAGAAGCGGTACCGGGCGCTTAAGCGCACAGCTGAGAGAGAACTTCTCATGAATGCAGATGT
  CATATGCTGCACATGTGTGGGTGCTGGTGACCCGAGGCTGGCCAAGATGCAGTTCCGTTCCATCCTCATT
  GACGAGAGCACCCAGGCCACTGAGCCTGAGTGCATGGTGCCTGTAGTCCTTGGGGCCAAGCAGCTAATCC
  TCGTCGGTGACCACTGCCAGCTGGGCCCAGTGGTGATGTGCAAGAAGGCAGCCAAGGCCGGACTGTCACA
  ATCGCTCTTCGAGCGCTTGGTGGTGCTGGGCATCCGGCCCATCCGCCTGCAGGTGCAATACCGCATGCAC
  CCTGCACTCAGCGCCTTTCCGTCCAACATCTTCTACGAGGGCTCATTGCAGAATGGCGTCACTGCAGCGG
  ATCGTGTCAAAAAAGGCTTTGACTTCCAGTGGCCACAACCTGACAAGCCTATGTTCTTCTACGTGACGCA
  GGGCCAGGAGGAGATTGCCAGCTCTGGCACATCCTACCTCAACAGGACGGAGGCAGCCAATGTGGAGAAG
  ATAACTACGAAGCTGTTGAAGGCAGGTGCAAAGCCTGACCAGATCGGCATCATCACCCCCTACGAGGGCC
  AGCGCTCTTACTTGGTGCAGTACATGCAGTTCAGCGGCTCCCTGCACACAAAGCTCTACCAGGAAGTGGA
  AATTGCCAGTGTGGACGCCTTCCAGGGCCGGGAGAAGGACTTCATCATTCTGTCCTGCGTGCGCGCCAAT
  GAACATCAGGGCATTGGGTTCCTAAACGACCCCCGGCGTCTGAATGTGGCTCTCACCAGAGCAAGATATG
  GCGTGATCATTGTGGGTAACCCAAAGGCCCTGTCGAAGCAGCCCCTGTGGAATCACCTGCTGAGCTACTA
  CAAGGAACAGAAGGCGCTAGTGGAAGGGCCGCTCAACAACCTACGTGAGAGCCTCATGCAGTTCAGCAAG
  CCTCGCAAACTTGTCAACACTGTCAACCCGGGTGCCCGCTTCATGACTACTGCCATGTACGATGCCCGTG
  AGGCCATCATCCCCGGGTCTGTCTATGACCGCAGCAGCCAGGGCCGGCCCTCGAACATGTACTTCCAGAC
  CCATGACCAGATTAGTATGATCAGCGCAGGCCCCAGCCACGTGGCTGCCATGAACATCCCTATTCCCTTC
  AACTTGGTCATGCCTCCCATGCCGCCACCTGGCTACTTCGGACAGGCCAACGGGCCGGCAGCTGGTCGGG
  GCACCCCAAAAACCAAGACTGGCCGTGGGGGCCGCCAGAAGAACCGCTTTGGGCTTCCTGGGCCCAGCCA
  GACCACCCTTCCCAACAGCCAGGCCAGCCAGGACGTGGCCTCCCAGCCCTTTTCACAGGGTGCCCTCACA
  CAGGGTTACGTGTCCATGAGCCAGCCCTCTCAGATGAGCCAGCCTGGCCTCTCCCAGCCAGAACTGTCCC
  AGGACAGCTACCTCGGTGATGAGTTTAAATCACAGATTGACGTGGCACTCTCACAAGACTCCACATACCA
  GGGAGAGCGGGCATACCAGCACGGCGGGGTCACCGGGCTGTCCCAGTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030680
• Insert Size: 3342 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030680.2, NP_109605.2
• RefSeq Size: 4598 bp
• RefSeq ORF: 3342 bp
• Locus ID: 19704
• UniProt ID: Q9EPU0
• Cytogenetics: 8 34.15 cM
• Gene Summary: RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) is shorter compared to isoform a.