Gucy2f (NM_001007576) Mouse Untagged Clone

CAT#: MC223603

Gucy2f (untagged) - Mouse guanylate cyclase 2f (Gucy2f), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gucy2f
• Synonyms: A930002I06; GC-F; GC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223603 representing NM_001007576
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTCGGACCCTGGCCTTTTTCTCGCCTTCTGTCCTGGTTTGCCATTTCCAGCAGACTGTCAGGAC
  AGCATGGCCTCCCTTCCTCCAAGTTCCTGAGATGTTTGTGCCTCTTGGCCCTTTTGCCTCTCCTTCGGTG
  GGGGCAAGCACTCCCCTACAAGATCGGGGTCATAGGTCCCTGGACATGTGATCCGTTCTTTTCCAAGGCC
  CTGCCTGAGGTTGCTGCTGCTTTAGCAATTGAGCGAATCAGCCGGGACAAGACATTTGACAGGAGTTACT
  CCTTCGAATACGTGATTCTTAACGAAGACTGTCAGACTTCAAAGGCCCTCGCCAGTTTCATTTCCCACCA
  GCAGATGGCCTCAGGATTTGTTGGGCCTGCCAACCCTGGCTTCTGCGAGGCAGCCTCACTCCTGGGAACC
  AGCTGGGACAAAGGGATTTTCTCTTGGGCTTGTGTGAATCATGAATTAGATAACAAACATAGCTTCCCAA
  CCTTCTCTCGGACACTCCCTTCTCCGATACGGGTGCTTGTAACCGTCATGAAGTATTTCCAGTGGGCACA
  TGCTGGGGTCATTTCCTCAGATGAAGACATTTGGATGCATACAGCCAATCGAGTCTCAAGTGCTCTTCGG
  AGCCAGGGCCTACCTGTAGGGGTCGTCCTGACCTCGGGACGAGACAGCCAGAGCATTCAGAAAGCTCTCC
  AGCAGATTCGCCAGGCAGACAGAATTCGCATAATCATAATGTGTATGCATTCAGCCTTGATTGGGGGAGA
  GACACAAACACACTTCTTGGAATTGGCTCATGATCTGAAAATGACAGATGGGACTTACGTCTTTGTCCCC
  TATGATGTACTGCTCTACAGTTTACCTTATAAGCACAGCCCCTACCAGGTCCTGAGGAACAATCCAAAGC
  TCCGGGAAGCCTATGATGCTGTGTTGACTATTACAGTGGAGTCCCATGAAAAGACCTTCTACGAAGCCTA
  TGCAGAAGCAGCAGCCCGCGGAGAAATTCCTGAGAAGCCTGACTCAAACCAAGTTTCACCGTTGTTTGGA
  ACCATCTACAATTCAATTTATTTTATCGCACAAGCCATGAATAATGCTATGAAAAAAAATGGACGGGCTA
  GTGCTGCCAGCCTGGTTCAGCATTCCAGAAACATGCAGTTCTATGGATTCAACCAGTTGATAAAGACAGA
  CTCAAATGGAAATGGGATTTCTGAATATGTAATCCTGGACACCAATGGGAAAGAATGGGAACTTCGTGGC
  ACCTACACTGTGGACATGGAAACTGAACTGCTAAGGTTCAGAGGGACCCCAATTCATTTCCCTGGCGGCA
  GACCTACTAGTGCAGATGCAAAATGCTGGTTTGCAGAAAGGAAGATCTGCCAAGGAGGTATTGACCCTGC
  CTTGGCCATGATGGTCTGCTTTGCTTTACTTATAGCCTTGCTGTCTATCAATGGATTTGCTTACTTTATA
  AGGCGACGTATAAATAAAATCCAGTTGATTAAAGGACCCAACAGAATCCTACTGACTTTGGAAGATGTGA
  CATTTATTAATCCCCACTTTGGCAGTAAGAGAGGAAGCCGTGCCAGTGTAAGCTTCCAGATCATCTCAGA
  AGTTCAAAGTGGGAGGTCCCCAAGGCTGTCCTTTTCTTCAGGTAGTCTAACTCCAGCTACATATGAGAAC
  TCCAACATAGCAATCTATGAGGGTGACTGGGTATGGCTGAAAAAGTTCCCTCCTGGAGATTTTGGAGACA
  TTAAGTCTATCAAATCGAGTGCAAGTGATGTGTTTGAAATGATGAAGGACCTGCGGCATGAAAATGTCAA
  CCCTCTATTGGGTTTCTTCTATGATTCGGGGATGTTTGCCATCGTGTCAGAATTTTGTTCTCGAAGGAGC
  CTAGAAGACATACTTACAAATGATGATGTGAAACTTGATTGGATGTTCAAATCATCACTACTGCTTGACC
  TCATCAAGGGGATGAAATACTTACACCACAGAGAATTTATTCACGGGAGGCTAAAGTCTCGGAACTGTGT
  GGTAGATGGGCGTTTTGTCCTAAAAGTGACAGATTATGGCTTTAATGACATTTTAGAAATGCTGAGACTC
  TCTGAAGAGGAACCTTCTGAAGAAGAGCTGCTGTGGACGGCCCCTGAACTGTTAAGAGCCCCTGGGGGCA
  TCAGGTTAGGTTCATTTGCAGGAGACGTCTATAGCTTTGCCATCATCATGCAAGAAGTGATGGTTCGGGG
  TGCTCCTTTCTGCATGATGGATCTGCCTGCCAAAGAAATCATAGACAGACTTAAAATGCCTCCCCCTGTG
  TACAGACCCGTGGTTTCTCCTGAGTATGCCCCTGCAGAATGTCTCCAGCTGATGAAGCAGTGCTGGGCTG
  AGGCGTCAGAACAGCGACCAACTTTTGATGAAATATTTAACCAGTTTAAAACTTTCAACAAAGGGAAAAA
  GACTAATATTATTGATTCTATGCTTCGGATGTTGGAGCAGTATTCTAGCAACTTGGAAGATTTGATCCGG
  GAACGGACTGAAGAATTGGAAATTGAAAAACAGAAAACGGAAAAGCTTCTAACACAGATGCTACCACTAT
  CAGTTGCTGAATCCCTCAAGAAGGGCTGTACAGTTGAACCTGAGGGATTTGACTTGGTCACCTTGTACTT
  CAGTGACATTGTGGGCTTCACCACCATCTCAGCCATGAGTGAGCCCATTGAAGTTGTGGACCTTCTGAAT
  GACCTGTACACACTCTTTGATGCCATCATTGGCAGTCATGATGTCTATAAAGTAGAGACCATTGGAGATG
  CCTACATGGTGGCCTCAGGCCTTCCCAAGAGGAATGGCAGTAGGCATGCAGCTGAGATTGCAAACATGTC
  CCTGGATATCCTGAGTTCTGTGGGTACCTTTAAGATGCGACACATGCCAGAGGTGCCAGTCCGAATTCGA
  ATAGGCCTGCACTCGGGGCCTGTTGTTGCTGGAGTGGTGGGCCTCACCATGCCCAGATATTGCTTGTTTG
  GGGACACTGTGAACACAGCTTCTCGAATGGAATCAACAGGGCTACCATACCGAATTCATGTCAGTCTCAG
  CACAGTAACAATTCTTCAAACTCTGAGTGAAGGCTATGAAGTAGAGCTTCGTGGAAGAACAGAGCTCAAG
  GGCAAAGGCACAGAGGAGACCTTTTGGCTTGTTGGAAAAAAGGGTTTCACCAAACCTCTTCCTGTACCCC
  CACCAGTGGGCAAAGATGGGCAAGTGGGCCATGGCTTGCAACCAGCAGAGATTGCAGCATTCCAAAGGAG
  AAAAGCAGAGAGGCAGTTGGTGCGAAACAAGCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007576
• Insert Size: 3327 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007576.2, NP_001007577.1
• RefSeq Size: 9998 bp
• RefSeq ORF: 3327 bp
• Locus ID: 245650
• UniProt ID: Q5SDA5
• Cytogenetics: X 62.85 cM
• Gene Summary: Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction (By similarity).[UniProtKB/Swiss-Prot Function]