Trpm8 (NM_134252) Mouse Untagged Clone

CAT#: MC223594

Trpm8 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpm8
• Synonyms: CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPP8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223594 representing NM_134252
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCGAGGGAGCCAGGCTCAGCATGAGGAGCCGCAGAAATGGTACTATGGGCAGCACCCGGACCC
  TGTACTCCAGTGTATCTCGGAGCACAGACGTGTCCTACAGTGACAGTGATTTGGTGAATTTTATTCAGGC
  AAATTTTAAAAAACGAGAATGTGTCTTCTTTACCAGAGACTCCAAGGCCATGGAGAACATATGCAAGTGT
  GGTTATGCCCAGAGCCAGCACATCGAAGGCACCCAGATCAACCAAAATGAGAAGTGGAACTACAAAAAAC
  ATACCAAGGAGTTTCCAACAGACGCCTTCGGGGACATTCAGTTTGAGACTCTGGGGAAGAAAGGCAAGTA
  CTTACGCTTGTCCTGTGACACCGACTCTGAAACTCTCTACGAACTGCTGACCCAGCACTGGCACCTCAAA
  ACACCCAACCTGGTCATTTCAGTGACGGGTGGAGCCAAAAACTTTGCTTTGAAGCCACGCATGCGCAAGA
  TCTTCAGCAGGCTGATTTACATCGCACAGTCTAAAGGTGCGTGGATTCTCACTGGAGGCACTCACTACGG
  CCTGATGAAGTACATAGGCGAGGTGGTGAGAGACAACACCATCAGCAGGAACTCAGAAGAGAACATCGTG
  GCCATTGGCATCGCAGCATGGGGCATGGTCTCCAACAGGGACACCCTCATCAGGAGCTGTGATGATGAGG
  GACATTTTTCAGCTCAATACATCATGGATGACTTTACCAGAGACCCTCTATACATCCTGGACAACAACCA
  TACCCACCTGCTGCTTGTGGACAACGGTTGTCATGGACACCCCACAGTGGAAGCCAAGCTCCGGAATCAG
  CTGGAAAAGTACATCTCTGAGCGCACCAGTCAAGATTCCAACTATGGTGGTAAGATCCCCATCGTGTGTT
  TTGCCCAAGGAGGTGGAAGAGAGACTCTAAAAGCCATCAACACCTCTGTCAAAAGCAAGATCCCTTGTGT
  GGTGGTGGAAGGCTCGGGGCAGATTGCTGATGTGATCGCCAGCCTGGTGGAGGTGGAGGATGTTTTAACC
  TCTTCCATGGTCAAAGAGAAGCTGGTACGCTTTTTACCACGCACTGTGTCCCGGCTGCCTGAAGAGGAAA
  TTGAGAGCTGGATCAAATGGCTCAAAGAAATTCTTGAGAGTTCTCACCTACTCACAGTAATTAAGATGGA
  AGAGGCTGGAGATGAGATTGTGAGCAACGCCATTTCCTATGCGCTGTACAAAGCCTTCAGCACTAATGAG
  CAAGACAAGGACAACTGGAATGGACAGCTGAAGCTTCTGCTGGAGTGGAACCAGTTGGACCTTGCCAGTG
  ATGAGATCTTCACCAATGATCGCCGCTGGGAGTCTGCCGACCTTCAGGAGGTCATGTTCACGGCTCTCAT
  AAAGGACAGACCCAAGTTTGTCCGCCTCTTTCTGGAGAATGGCCTGAATCTGCAGAAGTTTCTCACCAAT
  GAAGTCCTCACAGAGCTCTTCTCCACCCACTTCAGCACCCTAGTGTACCGGAATCTGCAGATCGCCAAGA
  ACTCCTACAATGACGCACTCCTCACCTTTGTCTGGAAGTTGGTGGCAAACTTCCGTCGAAGCTTCTGGAA
  AGAGGACAGAAGCAGCAGGGAGGACTTGGATGTGGAACTCCATGATGCATCTCTCACCACCCGGCACCCG
  CTGCAAGCTCTCTTCATCTGGGCCATTCTTCAGAACAAGAAGGAACTCTCCAAGGTCATTTGGGAGCAGA
  CCAAAGGCTGTACTCTGGCAGCCTTGGGGGCCAGCAAGCTTCTGAAGACCCTGGCCAAAGTTAAGAATGA
  TATCAACGCTGCTGGGGAATCGGAGGAACTGGCCAATGAATATGAGACCCGAGCAGTGGAGTTGTTCACC
  GAGTGTTACAGCAATGATGAAGACTTGGCAGAACAGCTACTGGTCTACTCCTGCGAAGCCTGGGGTGGGA
  GCAACTGTCTGGAGCTGGCAGTGGAGGCTACAGATCAGCATTTCATCGCTCAGCCTGGGGTCCAGAATTT
  CCTTTCTAAGCAATGGTATGGAGAGATTTCCCGAGACACGAAGAACTGGAAGATTATCCTGTGTCTATTC
  ATCATCCCCTTAGTGGGCTGTGGCCTCGTATCATTTAGGAAGAAACCCATTGACAAGCACAAGAAGCTGC
  TGTGGTACTATGTGGCCTTCTTCACGTCGCCCTTCGTGGTCTTCTCCTGGAACGTGGTCTTCTACATCGC
  CTTCCTCCTGCTGTTTGCCTATGTGCTGCTCATGGACTTCCACTCAGTGCCACACACCCCCGAGCTGATC
  CTCTACGCCCTGGTCTTCGTCCTCTTCTGTGATGAAGTGAGGCAGTGGTACATGAACGGAGTGAATTATT
  TCACCGACCTATGGAACGTTATGGACACCCTGGGACTCTTCTACTTCATAGCGGGTATTGTATTCCGGCT
  CCACTCTTCTAATAAAAGCTCGTTGTACTCTGGGCGCGTCATTTTCTGTCTGGATTACATTATATTCACG
  CTAAGGCTCATCCACATTTTCACCGTCAGCAGGAACTTGGGACCCAAGATTATAATGCTGCAGCGGATGC
  TGATCGACGTTTTCTTCTTCCTGTTCCTCTTTGCTGTGTGGATGGTGGCCTTTGGCGTGGCCAGACAGGG
  GATCCTAAGGCAAAATGAACAGCGCTGGAGATGGATCTTCCGCTCTGTCATCTATGAGCCCTACCTGGCC
  ATGTTTGGCCAGGTTCCCAGTGACGTGGATAGTACCACATATGACTTCTCCCACTGTACCTTCTCGGGAA
  ATGAGTCCAAGCCACTGTGTGTGGAGCTGGATGAGCACAACCTGCCCCGCTTCCCTGAGTGGATCACCAT
  TCCGCTGGTGTGCATCTACATGCTCTCCACCAATATCCTTCTGGTCAACCTCCTGGTCGCCATGTTTGGC
  TACACGGTAGGCATTGTACAGGAGAACAACGACCAGGTCTGGAAATTCCAGCGGTACTTCCTGGTGCAGG
  AGTACTGCAACCGCCTAAACATCCCCTTCCCCTTCGTTGTCTTCGCTTATTTCTACATGGTGGTGAAGAA
  GTGTTTCAAATGCTGCTGTAAAGAGAAGAATATGGAGTCTAATGCCTGCTGTTTCAGAAATGAGGACAAT
  GAGACTTTGGCGTGGGAGGGTGTCATGAAGGAGAATTACCTTGTCAAGATCAACACGAAAGCCAACGACA
  ACTCAGAGGAGATGAGGCATCGGTTTAGACAACTGGACTCAAAGCTTAACGACCTCAAAAGTCTTCTGAA
  AGAGATTGCTAATAACATCAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134252
• Insert Size: 3315 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134252.3, NP_599013.1
• RefSeq Size: 3869 bp
• RefSeq ORF: 3315 bp
• Locus ID: 171382
• UniProt ID: Q8R4D5
• Cytogenetics: 1 D
• Gene Summary: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.[UniProtKB/Swiss-Prot Function]