Srgap3 (NM_080448) Mouse Untagged Clone

CAT#: MC223576

Srgap3 (untagged) - Mouse SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srgap3
• Synonyms: AI452337; Arhgap14; D130026O08Rik; Gbi; MEGAP; mKIAA0411; WRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223576 representing NM_080448
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA
  TCCGCACGCAGCTGGTGGAGCAGTTCAAATGCCTGGAGCAGCAGTCCGAGTCGCGGCTGCAGCTGCTGCA
  AGACTTGCAGGAGTTCTTCCGCAGGAAAGCTGAGATTGAGCTGGAGTATTCCCGAAGCCTGGAGAAGCTG
  GCTGAGCGCTTCTCCTCCAAGATTCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGATCAATACCTCCTCT
  CGCCCGTGAACTGTTGGTATCTGGTACTGCATCAGACTCGGCGGGAGAGCCGAGACCATGCCACCCTCAA
  CGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAAGATGTCATCAGACTCTTCAAA
  AAGAGCAAAGAGATTGGCCTGCAGATGCATGAGGAGCTGCTGAAGGTCACCAACGAGCTCTACACAGTCA
  TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCAGAAAGCAAGCTGAAGGAGGCTGAGAAGCA
  GGAGGAGAAACAGTTTAACAAGTCAGGAGAGCTAAGCATGAACCTGCTCCGGCACGAGGACAGACCCCAG
  CGCCGCAGCTCCGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTATTCTGAGAACAAGC
  TCAAGTGCACGAAGGCCCGGAATGATTACCTGCTGAATCTGGCAGCCACCAACGCAGCTATCAGCAAATA
  CTACATCCACGATGTCTCTGATCTCATCGATTGCTGTGATTTGGGCTTTCACGCCAGCCTGGCCCGCACT
  TTTCGGACCTACCTCTCAGCAGAATATAACCTGGAGACCTCTCGCCATGAGGGTCTGGACGTCATAGAGA
  ATGCAGTGGACAACCTGGACTCCCGGAGTGACAAGCACACAGTCATGGATATGTGCAGCCAGGTCTTTTG
  CCCTCCACTCAAGTTTGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGTGCACAGCAGCCT
  GTCCAGACAGAGCTGCTCATGCGGTACCACCAGCTGCAGTCCAGGCTGGCCACTCTCAAGATTGAGAATG
  AAGAGGTTCGGAAAACCCTGGATGCCACCATGCAGACCTTACAGGACATGCTGACCGTGGAAGACTTCGA
  TGTCTCTGACGCCTTCCAACACAGCCGATCTACAGAGTCCATAAAGTCAGCTGCTTCTGAGACCTACATG
  AGCAAAATCAACATCGCCAAAAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTA
  AAGAATATGTGAATGGCAGTAACCTCATCACCAAGCTGCAAGCCAAGCATGACTTACTCAAGCAGACCCT
  GGGTGAAGGGGAGAGAGCAGAGTGCGGCACGACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG
  AGGAGACCTAGGCCTCTTTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCGTTCATTAAGG
  ATTCAGGACAAGCTATACCACTTGTAGTTGAGAGCTGTATTCGATTCATCAATTTATATGGACTCCAGCA
  GCAGGGGATCTTCAGAGTTCCAGGGTCTCAGGTTGAAGTCAATGACATCAAGAATTCCTTTGAGAGAGGT
  GAAGACCCCCTCGTGGATGACCAAAATGAGCGAGATATCAATTCAGTTGCTGGTGTTTTAAAACTGTATT
  TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA
  GAACCCAGCTGACAGGGTGCACCCAATCCAGCAGATCCTCATCACCCTGCCCCGCGTGGTCATTGTGGTC
  ATGAGATACCTCTTTGCTTTCCTCAATCACCTTTCCCAGTACAGTGATGAGAACATGATGGATCCCTACA
  ACTTGGCCATCTGCTTCGGGCCCACCCTCATGCACATCCCCGATGGGCAGGACCCTGTATCCTGCCAGGC
  GCATGTCAATGAAGTCATCAAAACTATCATCATCCACCATGAAGCCATTTTCCCCAGCCCTCGGGAGCTA
  GAGGGACCCGTGTACGAAAAATGCATGGCTGGAGGGGAAGAGTACTGTGACAGCCCACACAGTGAGCCGG
  GGACCATTGATGAAGTTGACCATGACAATGGCACGGAGCCTCACACCAGTGACGAAGAAGTGGAGCAGAT
  TGAGGCCATCGCCAAGTTTGACTATGTGGGGCGGTCCCCTCGTGAACTATCCTTCAAAAAAGGGGCCTCA
  CTACTCCTGTATCACCGTGCCTCAGAGGACTGGTGGGAGGGCCGCCACAACGGTGTAGATGGACTCATCC
  CTCATCAGTACATAGTTGTACAGGACATGGACGATGCCTTCTCCGATAGCCTGAGCCAAAAGGCTGACAG
  CGAGGCCAGCAGTGGGCCACTGCTGGATGACAAGGCCTCTTCCAAGAATGACCTCCAGTCCCCCACAGAG
  CACATCTCTGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGACTACGGTCCGACGGAGCAGCCATCC
  CCAGGCGCCGAAGTGGGGGCGACACACACAGCCCACCCCGGGGCTTGGGTCCCAGCATAGACACGCCACC
  CCGAGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCAGCCGGGGACGGATCGAGAGCCCTGAG
  AAGAGAAGGATGGCGACTTTCGGGAGCGCTGGCAGTATCAACTACCCTGACAAGAAGGCGCTGACAGAAG
  GGCTCTCCATGAGGTCGACTTGCGGTTCCACGCGACACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGC
  TGAGGCCCTGGCAGAAGACATCGAGAAGACCATGAGCACCGCTCTGCATGAGTTGCGGGAACTTGAGAGA
  CAGAACACTGTCAAGCAGGCACCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAATCCACCAGGCC
  CCATCAGCTCAGAGCCCGCCAGCCCCCTGCACACCATTGTCATCCGAGACCCTGATGCTGCTATGCGCCG
  AAGCAGCAGCTCATCGACGGAGATGATGACCACCTTCAAGCCAGCCCTGTCAGCTCGCCTGGCTGGTGCC
  CAGCTGCGTCCGCCCCCCATGCGTCCGGTCAGACCTGTGGTTCAGCACCGATCCAGCAGCAGCAGCAGCT
  CAGGTGTGGGCAGCCCGGCAGTGACACCCACGGAGAAGATGTTCCCTAATAGCTCCTCGGACAAGTCTGG
  CACCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080448
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080448.4, NP_536696.4
• RefSeq Size: 8887 bp
• RefSeq ORF: 3300 bp
• Locus ID: 259302
• UniProt ID: Q812A2
• Cytogenetics: 6 E3
• Gene Summary: GTPase-activating protein for RAC1 and perhaps CDC42, but not for RhoA small GTPase. May attenuate RAC1 signaling in neurons (By similarity).[UniProtKB/Swiss-Prot Function]