Pik3cg (NM_020272) Mouse Untagged Clone

CAT#: MC223553

Pik3cg (untagged) - Mouse phosphoinositide-3-kinase, catalytic, gamma polypeptide (Pik3cg), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3cg
• Synonyms: 5830428L06Rik; p110gamma; PI3Kgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223553 representing NM_020272
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGAACTATGAACAACCGGTGGTTCTAAGAGAGGACAACCTCCGCCGGCGCCGGAGGATGA
  AGCCACGCAGCGCAGCAGGCAGCCTGTCTTCCATGGAGCTCATCCCCATTGAGTTCGTACTGCCCACCAG
  CCAGCGCATCAGCAAGACTCCAGAAACAGCGCTGCTGCATGTGGCTGGCCATGGCAATGTGGAACAGATG
  AAAGCTCAGGTGTGGCTGCGCGCACTGGAGACCAGTGTGGCTGCGGAGTTCTACCACCGATTGGGCCCGG
  ACCAATTCCTCCTGCTCTACCAGAAGAAAGGACAATGGTATGAGATCTATGACAGGTACCAAGTGGTGCA
  GACCCTAGACTGCCTGCATTACTGGAAGTTGATGCACAAGAGCCCTGGCCAGATCCACGTGGTACAGCGA
  CACGTACCTTCTGAGGAGACCTTGGCTTTCCAGAAGCAGCTCACCTCCCTGATTGGCTATGACGTCACTG
  ACATCAGCAATGTGCACGATGATGAGCTAGAGTTCACTCGCCGCCGTCTGGTTACGCCCCGCATGGCTGA
  AGTGGCTGGCCGGGATGCCAAACTCTATGCTATGCACCCTTGGGTAACGTCCAAACCTCTCCCAGACTAC
  CTGTCAAAAAAGATTGCCAACAACTGCATCTTCATCGTCATCCACCGCGGTACCACCAGCCAAACCATCA
  AGGTCTCCGCAGATGATACTCCTGGTACCATCCTCCAGAGCTTCTTCACCAAGATGGCCAAGAAGAAGTC
  CCTAATGAATATCTCAGAAAGTCAAAGTGAGCAGGATTTTGTATTGCGGGTTTGTGGCCGCGATGAGTAC
  CTGGTGGGTGAAACACCCCTCAAAAATTTCCAGTGGGTGAGGCAGTGCCTCAAGAACGGAGATGAAATAC
  ACCTGGTGCTCGACACGCCTCCAGACCCAGCCCTTGATGAGGTGAGGAAGGAAGAATGGCCGCTGGTGGA
  TGACTGCACTGGAGTCACCGGCTACCACGAGCAGCTGACCATCCATGGCAAGGACCACGAGAGTGTGTTC
  ACAGTGTCTTTGTGGGACTGCGACCGAAAGTTCAGGGTCAAGATCAGAGGCATTGATATCCCTGTCCTGC
  CTCGGAACACCGACCTCACTGTGTTTGTGGAAGCGAACATCCAGCACGGGCAACAAGTCCTCTGCCAAAG
  GAGAACCAGCCCTAAGCCCTTCGCAGAAGAGGTACTCTGGAATGTGTGGCTGGAGTTTGGCATCAAAATC
  AAAGACTTGCCCAAAGGGGCTCTATTGAACCTACAGATCTACTGCTGCAAAACCCCATCACTGTCCAGCA
  AGGCTTCTGCAGAGACTCCAGGCTCCGAGTCCAAGGGCAAAGCCCAGCTTCTCTATTACGTGAACTTGCT
  GTTAATAGACCACCGTTTCCTCCTCCGCCACGGGGACTATGTGCTCCACATGTGGCAGATATCTGGCAAG
  GCAGAGGAGCAGGGCAGCTTCAATGCTGACAAGCTCACATCCGCAACCAATCCTGACAAGGAGAACTCAA
  TGTCCATTTCCATCCTGCTGGACAATTACTGTCACCCCATAGCTTTGCCTAAGCACCGGCCCACCCCTGA
  CCCAGAGGGAGACAGGGTTCGGGCTGAAATGCCCAATCAGCTTCGAAAGCAATTGGAGGCGATCATAGCC
  ACAGATCCACTTAACCCCCTCACAGCAGAGGACAAAGAATTGCTCTGGCATTTTCGATATGAAAGCCTGA
  AGCATCCGAAGGCTTACCCTAAGCTATTCAGCTCAGTGAAATGGGGGCAGCAAGAAATTGTTGCCAAAAC
  GTACCAGCTGTTAGCCAGAAGGGAGATCTGGGATCAAAGTGCTTTGGACGTTGGCTTAACCATGCAGCTC
  CTGGACTGCAACTTTTCAGACGAGAATGTCCGGGCCATTGCAGTTCAGAAACTGGAGAGCTTAGAGGACG
  ATGACGTTTTACATTACCTTCTCCAGCTGGTACAGGCTGTGAAATTTGAACCGTACCACGACAGTGCGCT
  GGCCAGATTCCTGCTGAAGCGTGGCTTGAGGAACAAAAGAATCGGTCACTTCTTGTTCTGGTTCCTGCGA
  AGTGAGATCGCACAGTCCAGACACTATCAGCAGAGGTTCGCTGTGATCCTGGAGGCGTACCTGCGAGGCT
  GTGGCACAGCCATGTTGCAGGACTTCACACAGCAGGTCCATGTGATTGAGATGTTACAGAAAGTCACCAT
  TGATATTAAATCGCTCTCGGCAGAGAAGTATGACGTCAGTTCCCAAGTTATTTCACAGCTTAAGCAAAAG
  CTTGAAAGCCTTCAGAACTCCAATCTCCCCGAGAGCTTTAGAGTTCCCTATGATCCTGGACTAAAAGCCG
  GTACCCTGGTGATCGAGAAATGCAAAGTGATGGCCTCCAAGAAGAAGCCCCTGTGGCTTGAGTTTAAGTG
  TGCTGATCCCACAGTCCTATCCAACGAAACCATTGGAATCATCTTTAAACATGGTGATGATCTGCGCCAA
  GACATGTTGATCTTGCAGATTCTACGCATCATGGAGTCCATTTGGGAGACTGAATCTCTGGACCTGTGCC
  TTCTGCCTTACGGTTGCATCTCAACTGGTGACAAAATAGGAATGATCGAGATTGTAAAGGATGCCACAAC
  GATCGCTCAAATTCAGCAAAGCACAGTGGGTAACACGGGGGCATTCAAAGATGAAGTCCTGAATCACTGG
  CTCAAGGAAAAATGTCCTATTGAAGAAAAGTTTCAGGCCGCAGTGGAAAGGTTTGTTTACTCCTGTGCAG
  GCTACTGTGTGGCCACATTTGTTCTTGGGATCGGTGACAGGCACAACGACAACATTATGATCTCAGAGAC
  AGGAAACCTATTTCATATAGACTTCGGACACATTCTTGGGAATTACAAGAGTTTCCTGGGCATCAATAAA
  GAGAGAGTGCCCTTCGTCCTAACCCCAGACTTCTTGTTTGTGATGGGATCTTCTGGAAAAAAGACAAGTC
  CACACTTCCAGAAATTCCAGGATGTCTGTGTTAGAGCTTACCTAGCTCTTCGCCATCACACAAACCTGTT
  GATCATCTTGTTCTCCATGATGCTGATGACAGGAATGCCCCAGCTGACAAGCAAAGAGGACATTGAATAT
  ATCCGGGATGCCCTCACCGTGGGAAAAAGCGAGGAGGACGCTAAGAAATATTTCCTTGATCAGATCGAAG
  TCTGCAGAGACAAAGGATGGACTGTGCAGTTTAACTGGTTCCTACATCTTGTTCTTGGCATCAAACAAGG
  AGAAAAGCACTCCGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020272
• Insert Size: 3309 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020272.2, NP_064668.2
• RefSeq Size: 6811 bp
• RefSeq ORF: 3309 bp
• Locus ID: 30955
• UniProt ID: Q9JHG7
• Cytogenetics: 12 A3
• Gene Summary: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contribute to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.