Pde3b (NM_011055) Mouse Untagged Clone

CAT#: MC223550

Pde3b (untagged) - Mouse phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde3b
• Synonyms: 9830102A01Rik; AI847709
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223550 representing NM_011055
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAAGACGAGCGCGAGCGGGACGCGCCAGCCATGAGGTCCCCGCCGCCGCCGCCAGCCTCGGCCG
  CCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCGGCAGGACCCTCC
  GCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCGCTCCGCGCCGGG
  GCACGCCTCTCGCTCGGCGTCCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGAGGCCCGAGCGCT
  GGGCGGCCGCGGCAGCCGGGCTCCGGACGCTGCTGAGCGCCTGCTCGCTCAGCCTCAGCCCGCTCTTCAG
  CATCGCCTGTGCCTTCTTCTTCCTCACCTGTTTCCTGACGCGCGCGCAGCGCGGCCCGGGCCGCGGCGCC
  GGCTCCTGGTGGCTGCTGGCGCTGCCCGCCTGCTGCTACCTGGGCGACTTCGCGGCGTGGCAGTGGTGGT
  CGTGGCTGCGTGGGGAGCCGGCGGCGGCGGGCCGGCTCTGCCTGGTGCTGAGCTGCGTGGGGCTGCTGAC
  GCTCGCGCCCCGCGTGAGGCTGCGGCACGGCGTCCTGGTGCTGCTCTTCGCCGGCCTGGTGTGGTGGGTG
  TCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGTGCCTGGTCGGGGGCGCGG
  GATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTCCCCTCCGCCGCGCTCGGC
  GAGTACCGCGGAGGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGCTGCGTGTCGCTGGGAGAA
  AGCGCAGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGCCTTGTATTTCCCGAGAAC
  AGATGATCCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCAGAATTCTGGAGGTGGAAA
  TGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTGCTGATTGACCCAAGCCTT
  CCCCCACAAGTCATTTCTTCTCTGCGGAGTATCAGTAGCTTGATGGGTGCTTTCTCAGGTTCCTGTAGGC
  CAAAGATTAATTCTTTTACACCATTTCCTGGATTTTATCCCTGCTCTGAAGTAGAAGATCCAGTTGAGAA
  AGGAGATCGAAAACTTCACAAGGGATTGAGTGGCAGAACCAGTTTCCCAACTCCACAGCTGAGGAGGAGC
  TCTGGAGCCTCATCGTTGCTGACTAATGAGCACTGTTCAAGGTGGGATCGCAGCAGTGGTAAGAGGTCTT
  ACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAGTTCAAATCTTTTTACAAT
  TCCAAAGCAGAGGTCATCGTCTGTGTCACTGACGCACCATGCAGGTCTGAGAAGAGCTGGTGCTTTGCCC
  AGCCACAGTCTTCTGAATTCTTCAAGCCATGTACCAGTGTCTGCTGGCTCTCTAACTAATCGATCACCCA
  TAGGATTTCCTGATACCACTGATTTTCTTACTAAGCCAAATATTATTTTACATAGATCACTGGGCAGTGT
  ATCAAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAATCTGTGTAGCAGCTGTGGACAC
  CAAATACTCAAATATGTTTCAACATGTGAACCCGATGGTACAGACCACCCCAGTGAAAAATCAGGTGAAG
  AAGACAGCAGTGTTTTCTCAAAAGAACCATTGAACATTGTGGAAACCCAAGAAGAAGAGACCATGAAGAA
  AGCCTGCAGGGAGTTATTTTTGGAAGGTGATAGTCACCTGATGGAAGAGGCACAGCAACCAAATATCGAT
  CAGGAAGTGTCACTGGATCCAATGTTAGTAGAAGATTATGATTCATTAATAGAAAAGATGAACAACTGGA
  ATTTTCAGATTTTTGAGCTTGTAGAAAAGATGGGAGAAAAATCAGGAAGGATTCTCAGTCAGGTTATGTA
  TACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAAGAATTTATGAATTATTTT
  CGTGCCTTAGAAAATGGCTACCGGGACATTCCATATCACAATCGTGTGCATGCCACAGATGTCCTACATG
  CTGTTTGGTATTTGACAACCCGACCAATTCCTGGCTTACCTCAGATCCATAATAACCATGAAACGGAAAC
  CAAAGCAGATTCAGATGGTAGACTTGGTTCTGGACAGATTGCTTACATTTCTTCGAAGAGTTGCTGTATT
  CCAGATATGAGTTATGGCTGCCTATCTTCAAACATCCCTGCATTAGAATTGATGGCTTTGTATGTGGCAG
  CTGCCATGCACGATTACGATCACCCAGGAAGAACAAATGCGTTTCTAGTGGCTACAAATGCACCTCAGGC
  AGTTTTATACAATGACAGATCTGTTCTAGAGAATCATCATGCTGCATCAGCTTGGAATCTGTATCTTTCT
  CGCCCAGAGTACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGGTTTCGATTTTTAGTTATAG
  AAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCAGAATTCAATGCCAAGGCCAATGA
  TGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGCCTCTTGGTCTGCCAGGTGTGCATCAAA
  TTAGCAGATATCAATGGCCCAGCAAAAGATCGGGACCTACATTTGAGATGGACAGAAGGCATTGTGAATG
  AATTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCCCTTCATGGATCGTTCTTC
  TCCTCAACTAGCAAAACTCCAAGAATCTTTTATCACCCACATTGTGGGCCCCCTGTGCAACTCCTATGAT
  GCTGCTGGCTTGCTGCCGGGTCAGTGGATAGAAACAGAAGAGGGTGATGATACAGAAAGTGATGATGATG
  ATGATGATGATGATGGTGGTGGTGAAGAATTAGATTCAGATGATGAAGAAACAGAAGACAATCTAAATCC
  TAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGCCAACTAATGCACCACCTCACTGAAAACCAC
  AAGATATGGAAGGAAATCATAGAAGAAGAAGAAGAAAAATGTAAAGCTGAGGGGAACAAACTGCAGGTGG
  ATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTTATTGAAGAAGCAGATGAAGAGGAAGAACAAAT
  GTTTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011055
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011055.2, NP_035185.2
• RefSeq Size: 5314 bp
• RefSeq ORF: 3300 bp
• Locus ID: 18576
• UniProt ID: Q61409
• Cytogenetics: 7 59.46 cM
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function]