Sh3pxd2a (NM_001164717) Mouse Untagged Clone

CAT#: MC223542

Sh3pxd2a (untagged) - Mouse SH3 and PX domains 2A (Sh3pxd2a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3pxd2a
• Synonyms: 2310014D11Rik; AA589508; AI256723; AI413738; C230050L11; EG329070; Fish; Gm5098; Sh3md1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223542 representing NM_001164717
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCCTACTGCGTGCAAGATGCCACCGTGGTGGACGTGGAGAAGCGGAGGAGCCCCTCTAAACACT
  ATGTATACATTATCAACGTGACCTGGTCTGACTCCACCTCCCAGACTATCTACCGGAGGTACAGCAAGTT
  CTTCGACCTGCAGATGCAGCTTCTGGATAAGTTTCCTATTGAAGGTGGCCAGAAGGATCCGAAGCAAAGG
  ATTATTCCCTTTCTTCCAGGCAAGATCCTCTTCCGGAGAAGCCACATCCGCGACGTGGCTGTGAAGAGAC
  TAAAGCCCATCGATGAATACTGCAGGGCGCTTGTCCGGCTGCCGCCCCACATTTCACAGTGTGACGAAGT
  CTTCCGGTTCTTTGAGGCACGGCCTGAGGATGTCAACCCTCCAAAAGAAGACTATGGCAGTTCCAAGAGG
  AAATCAGTGTGGTTGTCCAGCTGGGCTGAGTCTCCCAAGAAGGACGTGACAGGTGCCGACACCAACGCCG
  AGCCCATGATCCTGGAACAGTACGTGGTGGTGTCCAACTATAAGAAACAAGAGAACTCGGAGCTGAGCCT
  CCAGGCCGGGGAGGTGGTAGATGTCATCGAGAAGAACGAAAGCGGCTGGTGGTTTGTGAGCACATCTGAA
  GAGCAAGGTTGGGTCCCCGCCACCTACTTGGAGGCCCAGAATGGCACACGAGACGACTCGGACATCAACA
  CCTCCAAGACTGGGGAAGAAGAGAAGTATGTCACTGTGCAGCCCTACACCAGCCAGAGCAAAGACGAGAT
  CGGCTTCGAGAAGGGTGTCACCGTGGAGGTGATTCGAAAGAACCTGGAAGGCTGGTGGTACATCAGGTAC
  CTCGGCAAAGAGGGTTGGGCACCAGCGTCCTACCTGAAGAAGGCCAAGGATGACCTGCCGACCCGGAAGA
  AGAACCTGGCGGGTCCGGTGGAGATCATAGGGAACATTATGGAGATCAGCAACCTTCTCAACAAGAAGGC
  ATCTGGGGATAAGGAGGCTCCGGCTGAAGGCGAGGGATCCGAGGCCCCCATCACCAAGAAAGAGATCAGC
  TTACCGATCCTCTGCAACGCCTCCAATGGCAGCGCCTTGGCCATTCCCGAGAGGACCACATCCAAGCTAG
  CCCAGGGCTCCCCAGCTGTGGCCAGGATCGCCCCTCAGAGGGCCCAGATCAGCTCCCCAAATCTGAGGAC
  AAGACCTCCCCCGCGCAGAGAATCCAGCCTGGGGTTCCAGCTGCCAAAGCCGCCAGAGCCCCCTTCTGTT
  GAGGTAGAATACTACACCATTGCTGAATTCCAGTCCTGCATTTCTGACGGGATCAGCTTTCGAGGCGGAC
  AGAAGGCAGAGGTCATCGACAAGAACTCGGGTGGTTGGTGGTACGTGCAGATCGGGGAGAAGGAGGGCTG
  GGCCCCAGCCTCATACATTGACAAGCGCAAGAAACCCAACCTCAGCCGCCGAACCAGCACTCTGACGCGG
  CCCAAGGTGCCGCCACCTGCGCCCCCCAGCAAGCCTAAGGAGGCCGAGGAGAATCCTGTGGGTGCCTGTG
  AGAGCCAGGGCTCCCCACTGAAGGTTAAATACGAGGAACCCGAGTATGACGTCCCTGCCTTTGGCTTTGA
  CTCAGAGCCCGAGATGAATGAAGAGCCTTCAGGGGACAGAGGTTCAGGTGACAAGCATCCCGCCCAGCCC
  CGAAGGATCTCGCCTGCCTCTTCCCTGCAACGGGCCCATTTCAAGGTGGGTGAGTCTTCTGAGGACGTGG
  CCCTGGAAGAGGAGACCATCTATGAGAATGAGGGCTTCAGGCCATACACAGAAGACACCCTGTCTGCCAG
  AGGCTCCTCTGGGGACAGTGACTCCCCTGGGAGCTCCTCTTTGTCCCTTGCCGTGAAAAACTCCCCTAAA
  TCAGATTCCCCCAAATCCTCATCACTCCTAAAGCTCAAAGCAGAGAAGAATGCCCAGGCAGAACTGGGGA
  AAAACCAGTCCAACATCTCCTTCTCCTCCTCTGTCACCATCAGCACCACCTGTTCCTCCTCCTCATCCTC
  GTCCTCCTTGTCCAAGAACAATGGTGACCTGAAACCACGTTCTGCCTCAGATGCAGGTATCCGTGACACC
  CCTAAGGTTGGGACCAAGAAAGATCCTGATGTGAAGGCCGGGCTGGCCTCCTGCGCCCGAGCCAAGCCAT
  CCGTGAGACCAAAGCCAGTCCTGAACCGAGCGGAGTCTCAAAGCCAGGAGAAGATGGATATTAGTTCCTT
  ACGGCGCCAGCTGAGGCCCACAGGCCAGCTCCGGGGGGGCCTCAAGGGCTCTAGGAGTGAGGACTCAGAG
  CTGCCTCCACAGATGGCTTCTGAGGGATCCAGGCGAGGTTCTGCGGACATCATCCCTCTCACGGCCACCA
  CTCCCCCGTGTGTCCCCAAAAAGGAATGGGAAGGGCAAGGCGCCACCTACGTGACGTGCAGCGCCTATCA
  GAAGGTCCAGGACTCGGAGATCAGCTTCCCCGAAGGCGCCGAGGTGCACGTGCTGGAGAAGGCGGAAAGT
  GGGTGGTGGTACGTGAGGTTTGGGGAGCTGGAGGGCTGGGCTCCTTCCCACTACTTGGTGGCCGAGGAGA
  ACCAGCAACCTGACACAGCTAGCAAAGAGGGAGACACAGGAAAGAGCTCGCAGAACGAGGGCAAGTCAGA
  CAGCCTGGAAAAGATTGAGAAGCGTGTGCAGGCGCTCAACACTGTGAACCAGAGCAAGAGGGCCACCCCA
  CCCATCCCCTCGAAGCCTCCCGGGGGCTTCGGCAAGACCTCGGGCACCGTAGCGGTGAAGATGAGGAACG
  GGGTCCGGCAAGTGGCCGTCAGGCCCCAATCTGTGTTTGTGTCTCCGCCACCCAAGGACAACAACCTGTC
  CTGTGCCCTTCGGAGGAACGAGTCGCTAACGGCCACCGACAGCCTCAGAGGTGTCCGCAGGAACTCCTCC
  TTTAGCACCGCACGGTCAGCAGCCGCTGAGGCCAAGGGCCGCCTGGCCGAGCGGGCTGCCAGCCAGGGCT
  CAGAATCGCCCCTGCTGCCTACCCAGCGCAAAGGCATCCCTGTCTCCCCCGTGCGTCCCAAGCCCATAGA
  GAAGTCCCAGTTTATCCACAACAACCTCAAGGATGTGTACATCTCGATTGCAGACTATGAGGGGGACGAA
  GAGACGGCTGGCTTCCAGGAGGGGGTGTCCATGGAGGTGCTGGAGAAGAACCCCAATGGCTGGTGGTACT
  GCCAGATCCTGGATGAGGTGAAGCCCTTCAAGGGCTGGGTACCCTCCAACTACCTTGAGAAGAAGAACTA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164717
• Insert Size: 3291 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164717.1, NP_001158189.1
• RefSeq Size: 10380 bp
• RefSeq ORF: 3291 bp
• Locus ID: 14218
• UniProt ID: O89032
• Cytogenetics: 19 C3
• Gene Summary: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.