Mkl2 (NM_001122667) Mouse Untagged Clone

CAT#: MC223530

Mkl2 (untagged) - Mouse MKL/myocardin-like 2 (Mkl2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mkl2
• Synonyms: ENSMUSG00000075401; Gt4-1; mKIAA1243; MRTF-B; Mrtfb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223530 representing NM_001122667
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCACACAGGAGCCATAGACACAGAGGAAGAGCTGGGGCCTCTAGCCCATCTTGCACCAAGTCCTC
  AAAGTGAGTCTGTGGCTCACGAATTCCAGGAACTGTCTCTACAGTCCAGCCAGCACTTACCCACTCTGAA
  CGAACGGAAAAATGTGCTCCAGCTGAGGCTGCAACAAAGGAGGACAAGAGAACAACTAGTGGACCAGGGC
  ATCATGCCACCTTTGAAGAGTCCAGCAGCATTCCATGAGCAGATAAAAAGCCTGGAGCGAGCCAGAACTG
  AGAATTTTCTGAAGCACAAGATCCGGAGCCGCCCAGACCGCTCTGAGCTTGTCAGGATGCATATTTTAGA
  AGAAACGTTTGCAGAGCCATCTCTTCAGGCCACACAGATGAAGCTGAAGAGAGCTCGGCTGGCAGATGAC
  CTGAACGAGAAGATTGCTCAAAGGCCTGGTCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTGGATT
  CGAGTGTCAAGGAAGCGATTATAGGTGTGGTGAAGGAGGACTATCCTCACACTCATGGCGAGTTCTCATT
  TGATGAAGATAGCAGTGATGCTTTGTCTCCAGACCAGCCAGCGAGCCAGGAGTCTCAGGGCTCAGCTGCA
  TCCCCCAGTGAGCCAAAAGTCAGTGCCTCGCCACCTCCTGTGACTGCAAGCACTCCAGCCCAGTTTACCT
  CAGTGTCCCCAGCAGTTCCTGAATTCTTGAAAACCCCTCTAACTGCAGACCAGCCTCCCACAAGGTCTAC
  AGCTCCCGTGCTCCCTACAAACACTGTGTCCTCAGCGAAGTCTGGCCCAATGCTGGTGAAGCAAAGCCAT
  CCCAAGAATCCAAACGACAAACACCGTAGCAAAAAATGCAAAGATCCGAAACCGCGAGTGAAGAAGCTTA
  AGTACCACCAGTACATTCCACCCAACCAGAAGGGGGAGAAGAGCGAGCCACAGATGGACTCCAACTATGC
  CCGCCTGCTCCAGCAGCAGCAGCTCTTCCTACAGCTGCAGATCCTGAGCCAGCAGCAGCAGCAACAGCAG
  CAGCAGCACTACAACTACCAGACCATCCTACCTGCACCCATCAAAACTGACAAGAACAGCAGCAGCGGCA
  GTAACAGCGGCAGTAGCAGCAGCATGCCGGCGAGGAGGCCAGGACCGCTGCCCTCCAGCTTGGATGACTT
  AAAGGTGTCAGAGCTGAAGACAGAACTCAAACTGAGGGGTCTGCCAGTGTCAGGAACTAAACCAGACCTC
  ATTGAACGCCTGAAACCCTACCAGGAAGTGACCAGTAGTAACCTTGCCACTGGCAGTATTGTGGCAGTGT
  CATCAGCAACCATTGTCACCAGTAACCCAGAGGTCACAGTGGCACTGCCAGTCACAACACTGCATAATGC
  TGTAACAAGCTCTGTGTCCACTTTCAAGGCAGACTTGGCACTTCCTGCAACCAGCAGTGTGCCCCATGTT
  GAAAATGCCCACTCTCCTCTGCCCATCTCCCCATCACCCTCTGAGCAGTCCAGTCTCAGTACCGATGACA
  CAAACATGACAGACACCTTCACAGAGATCATGACTATGATGTCACCTTCCCAGTTGTTGTGTTCATCTCC
  CCTGAGGGTGGTGAGCCATGATGACAGCCTGAGCCCTTCCAGTAGCACCCTGTCTACTCTAGAACTGGAT
  GCTGCTGAGAAGGACCGCAAACTTCAGGAGAAGGAGAAACAGATTGAAGAACTGAAGAGGAAACTGGAAC
  AAGAACAGAAGCTTGTGGAGGTCCTCAAGATGCAGCTTGAGGTGGAAAAACGTGGGCAGCAGCGACCACC
  AGACCCTCAGCCCAGTGATCCCCCACATCCCTTTAATACATCAGATCCTAAACATGGCAGTGTTGGGTCC
  TCCATCAAGGATGAGGCCTCACTCCCTGACTGCTCCAGCCCCCAGCAGCCCATCACAGTGCCTGGCCACT
  CTGTAGGCCAGCCCATCTCCACGGGTAGCCAGACCCTTGTTGCCAAAAAGACTGTGGTCGTCAAGCAAGA
  GGTCCCCATGGCCCAAGCAGAGCAGCAGAACGTTGTCTCGCAGTTCTACCTTAGTTCCCAGGGACAGCCA
  CCTGCCCTTGTTGCTCAGCCTCAGGCCTTACTGACCACACAGACAACTCAGCTGCTGCTGCCAGTGTCCA
  TCCAGGGCTCAAATGTTACTTCAGTGCAACTCCCAGTAGGCAGCCTTCAACTCCAGACCCCAGCACAAGG
  AAGAGTCCAGGCTCAGCCTCATGTAGCTGCTGCCACACAGGTTCCAGCTGCAGCCCTGCCCTCAGCCTTG
  ACCTCAGCACTGCCTCAGAAGCAGGAAGCCTTCCCACAGCACGTGTTGGGCCAGCCTCAGCCAGTCAGAA
  AGGTCTTCACAAACTCGGCACCAAACACAGTTCTTCAGTATCAGAGGCAGCCTGGCCCAACGAACCAGCA
  GCCCTTTGTCAGTAAGACCTCTAACCCTGCTCTTCAATCCAGAACTGCCCCACTTGCACCCCTGCAAAAT
  GGACCCAGCCTAGCCAGCAAGCCTAGCTCACCACCCCCACCCCAGCAGTTTGTTGTTCAGCACTCTTTGT
  TTGCAACCCCAATCACGAAGACAAAAGATCCACCTCGATATGAGGAAGCCATCAAGCAGGCACGTAGCAC
  ACAGCCAGCCCTTCCAGAGGTTTCCAGTGTGCACAGTCAGCAGATGGACGACCTCTTCGACATCCTCATA
  AAGAGTGGAGAGATTTCCTTCCCAATAAAAGAAGAGCCTTCTCCAATTTCCAAGATGAAACCAGTGACAG
  CCAGCATCACCACAATGCCAGTCAATACCGTGGTCTCTCGACCACCGCCCCAGGTCCAAATAGCACCACC
  TGTATCCTTAGAACCTGTGAACAGTTTATCTGCCAGCTTAGAGAACCAGCTAGAAGCCTTCTTGGATGGC
  ACTTTGCCCTCGGCCACTGACACTGGCCCACTGCAGAACAGCAGTGAAGATAGAGAGTCTTTCTCTCTGA
  TCGAGGATCTGCAGAACGACTTGCTGAGTCATTCCAGCATGCTGTACCAGTCTCACTCTCCCATGGAGAC
  CTCTGAGGCCCAGTTGGTGTCAGGGACCCCCTGTCTATCTCTTGACCTGTCAGACTCCAACCTGGACAAC
  ATGGAGTGGCTAGACATTACCATGCCCACCACATCCTCCGGGCTCACTCCACTGAGTACCACTGCGCCAA
  GCATGTTCTCTGCTGATTTTCTGGACCCACAGGACCTGCCGCTGCCATGGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122667
• Insert Size: 3276 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122667.2, NP_001116139.1
• RefSeq Size: 8281 bp
• RefSeq ORF: 3276 bp
• Locus ID: 239719
• Cytogenetics: 16 A1
• Gene Summary: Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.[UniProtKB/Swiss-Prot Function]