Unc13d (NM_001009573) Mouse Untagged Clone

CAT#: MC223479

Unc13d (untagged) - Mouse unc-13 homolog D (C. elegans) (Unc13d), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unc13d
• Synonyms: 2610108D09Rik; Jinx; mFLJ00067; Munc13-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223479 representing NM_001009573
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACACACCTTTCCCACCCCCAGCGGCGTCCACTCTTGCGCCAGGCCATCAAGATAAGGCGCCGCA
  GGGTCAGAGACCTGCAGGATCCACCACCCCAAGCCACCCAGGAGGTCCAGGTTCAGTCCCACCATTTCTC
  CCCAGAGGAGCGGGACCTGCTGTATGAGGAAGCCCTCTATACTGTCCTGCACCGCCTGGGTCAGCCAGAG
  CCCAACCATGTGAAGGAGGCCTCGGAACTGCTGAGCTATCTGCAGGAGGCTTTCCAGGTGCAGCCTGAGG
  AGCACCAGCAGATGCTGCGGCGCGTCAGGGAGCTCGAGAAGCCAGTATTTTGCCTGAAGGCCACAGTGAA
  ACAGGCCAAGGGTATTCTGGGAAAAGATGTCAGCGGGTTCAGCGACCCCTACTGCCTGCTGGGCATCGAG
  CAGAAGGTTGGTGTAGCAGAGGGCAGCCCTGTGTCCCGACGTCGGCAGAAGGCAGTGGTGAAGCACACCA
  TCCCGGAAGAGGAGACCCATCGCACCCAAGTCAAGAGCCAGACACTTAATCCCGTCTGGGACGAGACCTT
  TATCTTGGAGTTTGAGGACATAGCCAATGCAAGCTTTCACCTGGACATGTGGGACCTGGACACTGTGGAG
  TCTGTCAGGCAGAAGCTCGGGGAGCTCACGGACCTGCACGGGCTCCGGAGGATCTTTAAAGAAGCTCGGA
  AGGATAAAGGCCAGGACGACTTTCTGGGGAATGTGGTTCTGAGGTTGCAGGACCTGCGCTGCCGAGAGGA
  CCAGTGGTTCCCGCTAGAGCCCTGCACAGAGACCTACCCAGACCGCGGCCAGTGCCACCTTCAGTTCCAG
  TTCATTCACAAGAGGAGAGCCACGGCGGCCAGCCGCTCTCAGCCCAGCTACACTGTACACTTTCACCTAC
  TGCAGCAGCTGGTGTCCCATGAAGTCACACAGCACCAGGCCGGCAGTACCTCCTGGGACGCATCACTGAG
  TCCCCAGGCTGTCACCATCCTCTTTCTCCACGCCACTCAGAAGGACCTGTCGGACTTCCACCAGTCCATG
  GCGCAGTGGTTGGCCTACAGCCGCCTCTACCAGAGCCTGGAGTTCCCCAGCAGCTGCCTCCTGCACCCCA
  TCACCAGCATAGAGTACCAGTGGATCCAGGGCCGACTCAAAGCAGAACAGCGGGAGGAGCTGGCCACCTC
  CTTCACATCCCTGTTGGCCTATGGCCTCTCCCTTATCCGGAAGTTCCGCTCCGTCTTTCCCCTGTCTGTC
  TCTGACTCCCCATCCAGGCTGCAGTCCCTCCTCAGAGTCTTGGTCCAGATGTGCAAAATGAAGGCCTTTG
  GAGAACTGTGCCCAGACAGCGCTCCACTGTCCCAGCTGGTTTCTGAAGCTCTGCGGATGGGCACAGTTGA
  GTGGTTTCACCTGATGCAGCAACACCATCAGCCCATGGGCATCCTGGAGGCTGGCAAGGCCTTGCTAAAT
  CTGGTACAGGACGTCATGGGTGATCTGTACCAGTGTCGTCGCACATGGAACAAGATTTTCCACAATGTCC
  TCAAGATAGACCTGTTCTCCATGGCCTTCCTGGAGCTGCAGTGGCTGGTGGCCAAGAGGGTACAGGACCA
  CACGGTGGCGGCTGGCAACCTTGTTTCTCCAGATATTGGAGAGAGTCTGTTTCAGCTGTATGTCAGCCTG
  AAGGAGCTCTGCCAGCTGGGCCCTGTCCCCTCAGACAGCCGTGAAGTCCTGGCCCTGGATGGCTTCCACC
  GCTGGTTTCAGCCAGCCATCCCTTCCTGGCTGCAGAAGACTTACAGTGTCGCTCTGGAGCGGGTGCAGCG
  TGCCGTACAGATGGACACGCTGGTACCCCTGGGCGAACTGACCAAGCACAGCACTTCTGCCGTGGATCTG
  TCTACCTGCTTTGCCCAAATTAGCCACACTGCCCGGCAGCTGGACTGGCCAGACCCAGAGGAGGCCTTCA
  TGATCACTGTCAAGTTCGTGGAGGACACGTGCCGGCTGGCCCTGGTCTACTGTAGCCTTATAAAGGCCCG
  GGCCCGAGAGCTGTCTGCAGTTCAGAAGGACCAGAGCCAGGCAGCTGACATGCTGTGTGTGGTGGTAAAT
  AACATGGAGCAACTACGGTTGATCATCGACAAGCTACCCACTCAGCTGGCATGGGAGGCATTGGAGCAGC
  GGGTCGGGGCCGTGTTGGAGGAGGGGCAGCTGCAGAACACGTTACATGCTCAGCTGCAGGGCGCCTTGGC
  GGGGCTGGGCCATGAGATCCGTACTGGTGTCCGTACCCTGGCAGAGCAGTTGGAGGTGGGCATTGCCACA
  CACATCCAGAAACTCATTGGCGTCAAGGAGTCTGTTCTGCCCGAGGATGCCATTCTGCCCCTGATGAAAT
  TCTTGGAGGTGAAGCTTTGCTACATGAACACCAACCTGGTCCAGGAGAACTTCAGCAGCCTTCTGACTCT
  GTTGTGGACCCACACGCTTACTGTGCTGGTGGAGGTGGCTTCTTCCCAGCGTAGCTCGTCCCTGGCTTCT
  GGCAGGCTGAAGGTCGCCCTTCAGAACCTGGAGGTCTGCTTCCACGCTGAGGGCTGTGGTCTGCCACCAG
  AGGCCCTGCACACAGACACCTTCCAGGCTCTGCAGAACGACCTGGAGCTGCAGGCGGCCTCCAGCCGGGA
  GCTTATCCAGAAGTACTTCTGCAGCCGAATCCAGCAGCAGGCCGAAACCACTTCTGAGAGGCTGGGCGCA
  GTCACCGTCAAGGTCTCCTACCGCGCCTCTGAGCAGAGGCTTCGCGTGGAACTGCTCAGTGCTTCTAGCC
  TGCTGCCCCTGGACTCCAATGGTTCCAGTGACCCCTTTGTTCAGTTGACACTGGAACCCAGACATGAATT
  CCCTGAAGTGGCCCCCCGGGAGACCCAGAAGCACAAGAAGGAACTTCACCCACTCTTTGATGAGACCTTT
  GAATTCCTGGTGCCTGCTGAGCCTTGCCAAAAAGCCTGGGCATGCCTCCTGCTCACTGTGCTGGACCACG
  ACAGACTGGGAGCAGACGACCTGGAGGGAGAGGCCTTCTTACCGCTCTGCAGGGTACCTGGACTGACGGA
  CTGTGCAGAGCCGGGCGAAGCACCTCAAATGCGCCTGCCTCTCACATACCCTGCCCCCAACGGGGACCCA
  ATTCTGCGGCTGTTGGAGAGCCGGAAGGGGGATCGCGAGGCCCAGGCCTTTGTAAAGCTGAGGAGGCAGA
  GAGCCAAGCAGGCCTCCCAACATGCCCCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009573
• Insert Size: 3252 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009573.2, NP_001009573.2
• RefSeq Size: 3967 bp
• RefSeq ORF: 3252 bp
• Locus ID: 70450
• UniProt ID: B2RUP2
• Cytogenetics: 11 E2
• Gene Summary: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis (By similarity). Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.[UniProtKB/Swiss-Prot Function]