Cacna2d1 (NM_009784) Mouse Untagged Clone

CAT#: MC223473

Cacna2d1 (untagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant e, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacna2d1
• Synonyms: Ca(v)alpha2delta1; Cac; Cacna2; Cchl; Cchl2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223473 representing NM_009784
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTGGGCTGATCGGCCCCTCGAGCG
  AGGAGCCCTTCCCTTCGCCCGTCACTATCAAGTCATGGGTGGACAAGATGCAAGAAGACCTTGTCACACT
  GGCAAAAACAGCAAGTGGAGTGACTCAGCTTGCTGATATTTATGAAAAATACCAAGATTTGTATACTGTG
  GAGCCCAACAATGCACGCCAACTGGTTGAAATTGCAGCCCGAGACATTGAGAAGCTTCTAAGCAACAGAT
  CTAAGGCCCTGGTGCGCCTGGCTATGGAAGCAGAGAAAGTCCAAGCAGCCCACCAATGGAGGGAAGATTT
  TGCAAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCA
  GGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATG
  CAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAG
  TGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGC
  GCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTG
  ATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCT
  GGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTA
  GAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTT
  TCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGC
  AAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCC
  AGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTG
  CCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGG
  ACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGA
  ATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAG
  TTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTT
  CAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTT
  GATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTG
  CAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCC
  AGTCACACTGGATTTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATA
  GATGGAGAAAGTGGAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAG
  GAAATCGAACATACACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTA
  CAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATATTCAGAAACCCTGAAG
  CCAGACAATTTTGAAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTT
  CAGATAATAACACTGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTC
  CTGTAATACAGATTTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTAT
  TGGAGTAAGCAGAAAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAG
  TTTATCCCAAAGAGGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACG
  GAGCCTAGATAACGATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAA
  TCTGGAATTATGGTAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGG
  GAATTAAAATTGATGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGG
  TCCAGTTTGTGACTGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTT
  TTGATGGCAAATCACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGA
  TGAGACACCTGGTTAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCC
  AGGGGCAGCACCAAAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAG
  ATTGGCTGGTGGGCCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCAC
  GGCTCCTTGAGGCAGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCAC
  AGAACAAACCCAGTACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGT
  TCCAGGATCTTTCATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGA
  CATGTCCGTGTGACACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACAT
  GGTCAAGCAGCCCAGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGGAGGATTATACT
  GACTGTGGTGGTGTTTCTGGGTTAAACCCTTCCTTATGGTCTATCTTTGGACTCCAGTTTATACTCCTTT
  GGCTGGTATCTGGCAGCAGACACTACCTACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009784
• Insert Size: 3255 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009784.2, NP_033914.1
• RefSeq Size: 7415 bp
• RefSeq ORF: 3255 bp
• Locus ID: 12293
• UniProt ID: O08532
• Cytogenetics: 5 6.56 cM
• Gene Summary: This gene encodes a regulatory component of the voltage-dependent calcium channel complex. The product of this gene is a proprotein that is proteolytically processed into alpha-2 and delta subunits, which are linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (e) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (e) is shorter than isoform a, and is processed into alpha-2e and delta-1 subunits. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.