Lats2 (NM_015771) Mouse Untagged Clone

CAT#: MC223308

Lats2 (untagged) - Mouse large tumor suppressor 2 (Lats2), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lats2
• Synonyms: 4932411G09Rik; AV277261; AW228608
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223308 representing NM_015771
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCAAAGACTTTTCCTGCCACAACTTACTCTGGAAATAGCCGGCAGCGATTGCAAGAGATTCGAG
  AGGGGCTGAAGCAGCCATCCAAGGCTTCCACCCAGGGGCTGCTGGTGGGACCAAACAGTGACACTTCCCT
  GGATGCCAAAGTCCTGGGGAGCAAAGATGCCTCCAGGCAGCAGCAAATGAGAGCCACCCCGAAGTTTGGA
  CCTTATCAAAAAGCTCTCAGGGAAATCCGATATTCCCTCCTGCCTTTTGCCAACGAGTCAGGCACTTCGG
  CAGCTGCAGAGGTGAACCGGCAGATGCTTCAGGAGTTGGTGAATGCGGGATGTGACCAGGAGATGGCTGG
  CAGAGCGCTCAAGCAGACGGGCAGTAGGAGTATCGAAGCTGCCTTGGAGTACATCAGTAAGATGGGCTAC
  CTGGACCCCAGGAATGAGCAGATTGTGCGAGTCATCAAGCAGACCTCCCCAGGAAAGGGCCTGGCGCCCA
  CCCCGGTGACTCGGCGGCCCAGTTTCGAGGGCACAGGGGAAGCACTCCCATCCTACCACCAGCTGGGTGG
  TGCAAACTACGAGGGCCCCGCCGCACTGGAGGAGATGCCGCGGCAATATTTAGACTTTCTCTTCCCTGGA
  GCCGGAGCCGGCACCCACGGTGCCCAGGCTCACCAGCATCCTCCCAAAGGGTACAGCACAGCAGTAGAGC
  CAAGTGCGCACTTTCCGGGCACACACTATGGTCGTGGTCATCTACTATCGGAGCAGCCTGGGTATGGGGT
  GCAGCGCAGTTCCTCCTTCCAGAACAAGACGCCACCAGATGCCTATTCCAGCATGGCCAAGGCCCAGGGT
  GGCCCTCCCGCCAGCCTCACCTTTCCTGCCCATGCTGGGCTGTACACTGCCTCGCACCACAAGCCGGCGG
  CTACCCCACCTGGGGCCCACCCATTACATGTGTTGGGCACCCGGGGTCCCACGTTTACTGGCGAAAGCTC
  TGCACAGGCTGTGCTGGCACCGTCCAGGAACAGCCTCAATGCTGACTTGTACGAGCTGGGCTCCACGGTG
  CCCTGGTCTGCAGCTCCACTGGCACGCCGCGACTCGCTGCAGAAGCAGGGTCTAGAAGCCTCGCGGCCGC
  ATGTGGCTTTTCGGGCTGGCCCCAGCAGGACCAACTCCTTCAACAACCCACAACCTGAGCCCTCACTGCC
  CGCCCCCAACACGGTCACCGCCGTGACGGCCGCACACATCCTTCACCCTGTGAAGAGCGTGCGTGTGCTG
  CGGCCCGAGCCCCAGACAGCCGTGGGGCCCTCGCACCCCGCCTGGGTGGCTGCGCCCACAGCACCTGCCA
  CTGAGAGCCTGGAGACGAAGGAGGGCAGCGCAGGCCCACACCCGCTGGATGTGGACTATGGCGGCTCCGA
  GCGCAGGTGCCCACCGCCTCCGTACCCAAAGCACTTGCTGCTGCCCAGTAAGTCTGAGCAGTACAGCGTG
  GACCTGGACAGCCTGTGCACCAGTGTGCAGCAGAGTCTGCGAGGGGGCACTGAGCAAGACAGGAGTGACA
  AGAGCCACAAAGGTGCGAAGGGAGACAAAGCTGGCAGAGACAAAAAGCAGATTCAGACCTCCCCGGTGCC
  TGTCCGCAAGAATAGCAGAGATGAAGAGAAGAGAGAGTCTCGCATCAAGAGTTACTCCCCTTATGCCTTC
  AAATTCTTCATGGAGCAACACGTGGAGAATGTCATCAAAACCTACCAGCAGAAGGTCAGCCGGAGGCTAC
  AGCTGGAGCAGGAAATGGCCAAAGCTGGGCTCTGTGAGGCCGAGCAGGAGCAGATGAGGAAGATCCTCTA
  CCAGAAGGAGTCTAACTACAACCGGCTGAAGAGGGCCAAGATGGACAAGTCCATGTTTGTGAAAATCAAG
  ACTCTAGGCATCGGTGCCTTTGGGGAAGTGTGCCTCGCTTGTAAGCTGGACACTCACGCTCTGTACGCCA
  TGAAGACTCTCAGGAAGAAGGATGTCCTGAACCGGAATCAAGTGGCCCATGTCAAGGCTGAGAGGGACAT
  CCTGGCTGAAGCAGACAATGAGTGGGTGGTCAAACTCTACTACTCCTTCCAGGACAAGGACAGCCTGTAC
  TTTGTGATGGACTACATACCAGGCGGGGATATGATGAGCCTGCTGATCAGGATGGAGGTCTTCCCTGAGC
  ACCTGGCCCGCTTCTACATTGCAGAGTTGACCCTGGCCATTGAAAGTGTCCACAAGATGGGCTTTATCCA
  CCGGGACATCAAGCCTGACAACATACTCATCGACCTGGATGGTCATATTAAGCTGACAGATTTTGGCCTC
  TGCACTGGATTCAGGTGGACTCACAATTCCAAGTACTACCAGAAAGGGAACCACATGAGACAGGACAGCA
  TGGAGCCCGGTGACCTCTGGGACGATGTTTCCAACTGTCGCTGTGGAGACAGGTTAAAGACCCTGGAGCA
  GAGGGCGCAGAAGCAGCACCAGAGGTGCCTGGCACATTCTCTTGTCGGGACACCAAATTACATCGCTCCG
  GAGGTGCTTCTCCGCAAAGGGTACACGCAGCTCTGTGACTGGTGGAGCGTCGGTGTGATTCTCTTTGAGA
  TGCTGGTTGGGCAGCCGCCTTTCTTGGCCCCCACCCCCACAGAGACGCAGCTGAAGGTGATCAACTGGGA
  GAGCACGCTGCATATCCCTACGCAGGTGAGGCTCAGCGCTGAGGCCCGAGACCTCATCACGAAGCTGTGC
  TGCGCGGCTGACTGCCGCCTGGGCAGGGATGGGGCAGATGACCTCAAGGCACACCCGTTCTTCAACACCA
  TCGACTTTTCCCGTGACATCCGAAAGCAGCCTGCACCCTACGTCCCCACCATCAGCCACCCCATGGACAC
  CTCCAATTTTGACCCGGTGGATGAAGAAAGCCCCTGGCACGAGGCCAGCGGAGAGAGCGCCAAGGCCTGG
  GACACGCTGGCCTCCCCCAGCAGCAAGCATCCAGAGCACGCCTTCTATGAGTTCACCTTCCGCAGGTTCT
  TCGATGACAACGGCTATCCCTTCCGGTGCCCGAAGCCCTCAGAGCCCGCAGAGAGTGCAGACCCAGGGGA
  TGCGGACTTGGAAGGTGCGGCCGAGGGCTGCCAGCCGGTGTACGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_015771
• Insert Size: 3129 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015771.2, NP_056586.2
• RefSeq Size: 5213 bp
• RefSeq ORF: 3129 bp
• Locus ID: 50523
• UniProt ID: Q7TSJ6
• Cytogenetics: 14 C3
• Gene Summary: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (A) represents the longer transcript and it encodes the longer protein (isoform 1).