Atp12a (NM_138652) Mouse Untagged Clone

CAT#: MC223291

Atp12a (untagged) - Mouse ATPase, H+/K+ transporting, nongastric, alpha polypeptide (Atp12a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp12a
• Synonyms: Atp1al1; cHKA; HKalpha2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223291 representing NM_138652
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGGAAAACAGAAATCTATTCTGTAGAGCTCAATGGAACTAAAGACGTCGAGCTGGCAGACCAGA
  AAGATGACAAAAAGTTCAAGGGCGGGAAGAACAAAGACTCAGAACCCAACAAGAGTCAGGAGGAGGAGCT
  GAAGAAAGAACTTGATCTGGATGACCACAGACTCAGTAATACAGATTTGGAACAGAAATATGGTACAAAC
  ATCATCCAGGGTCTCTCCAGTATCAGAGCTGCAGAGCTCCTGGCTCGGGATGGGCCCAACGCCCTCACCC
  CTCCCAAGCAAACTCCAGAGATCATCAAGTTCCTCAAACAGATGGTGGGGGGCTTTTCCATCCTTCTGTG
  GATAGGTGCAGCCTTGTGCTGGATCGCATATGTGATCCAGTATGTCAGCAGTACTGCATCCCTGGACAAT
  GTCTATTTGGGCGCCATACTTGTCCTGGTCGTTATTTTAACGGGGATTTTTGCTTATTACCAAGAAGCCA
  AAAGTACCAACATTATGGCAAGCTTTAGTAAGATGATCCCCCAGCAAGCTCTTGTCATCCGAGATGCAGA
  AAAGAAGATCATTCCAGCAGAGCAGCTGGTGGTGGGGGACGTTGTGGAGATTAAAGGAGGGGACCAGATC
  CCTGCAGACATCAGACTGGTGTTTTCCCAAGGGTGCAAAGTAGATAACTCCTCTCTCACTGGGGAGTCTG
  AGCCCCAGGCTCGGTCAACTGAGTTTACCCATGAAAACCCCTTGGAAACTAAGAACATAGGCTTCTATTC
  TACAACGTGCCTGGAAGGTACGGCAACTGGCATTGTCATCAACACGGGTGACCGCACCATCATCGGGCGC
  ATAGCCTCCTTGGCTTCAGGCGTTGGGAGTGAGAAGACACCCATAGCCATTGAGATTGAGCATTTTGTCC
  ATATTGTGGCAGCAGTGGCTGTGTCCGTTGGCGTCATTTTCTTCATCACCGCCGTGTGCATGAAGTACTA
  CGTCCTGGATGCCATCATCTTCCTCATTAGCATCATTGTGGCCAATGTGCCAGAGGGCCTCCTGGCTACT
  GTCACTGTTACCCTGTCACTGACGGCAAAGCGGATGGCTAAGAAGAACTGCTTGGTAAAGAACCTGGAGG
  CCGTGGAGACGCTTGGCTCCACCTCCATCATCTGCTCAGATAAGACAGGGACCCTCACCCAGAACAGGAT
  GACTGTGGCCCACTTGTGGTTTGACAACCAGATCTTTGTGGCTGATACAAGTGAAAACCAGACAAAACAA
  GCCTTTGACCAAAGCTCTGGAACCTGGGCTTCCTTGTCCAAGATAATAACACTGTGTAACCGAGCAGAGT
  TCAGGCCAGGCCAGGAGAGTGTCCCCATCATGAAGAGAGTTGTGGTTGGAGATGCCTCAGAAACTGCTCT
  GCTAAAGTTCTCAGAGGTTATTTTGGGTGATGTGATGGACATTAGGAAAAGAAACCACAAAGTAGCTGAG
  ATTCCTTTTAATTCTACCAACAAATTTCAGCTCTCCATACATGAGACCGAGGACCCGAATGACAAGCGCT
  TCCTCATGGTGATGAAAGGGGCCCCTGAGAGGATCTTAGAGAAGTGCAGCACCATCATGATCAACGGCCA
  GGAGCAGCCACTGGACAAGAGCTCTGCCGATGCCTTCCACACGGCCTACATGGAGCTGGGTGGTCTGGGA
  GAGCGTGTGTTGGGTTTCTGTCACCTCTACTTGCCAGCAGATAAGTTTCCCCAAAGCTACACATTTGATG
  TAGACTCCATAAACTTTCCCACTTCCAATCTCTGCTTTGTGGGGCTTCTGTCCATGATTGACCCTCCTCG
  GTCTACCGTGCCAGATGCGGTCTCCAAATGTCGGAGTGCGGGGATCAAGGTTATCATGGTCACAGGTGAT
  CACCCTATCACAGCCAAAGCTATTGCCAAGAGTGTAGGAATCATATCAGCCAACAATGAGACGGTGGAAG
  ACATCGCAAAACGCCGCAACATTGCTGTGGAGCAAGTCAACAAAAGAGAAGCGAAAGCCGCTGTGGTGAC
  TGGCATGGAACTGAAGGACATGACCCCAGAACAACTGGATGAGCTTCTAATCAACTACCAAGAAATTGTG
  TTTGCCCGGACATCACCCCAGCAGAAGTTGATCATTGTAGAAGGCTGTCAGAGGCAGGATGCAGTTGTTG
  CTGTGACGGGTGATGGAGTAAACGACTCTCCAGCATTAAAGAAGGCAGACATCGGGATTGCTATGGGGAT
  AGCAGGTTCTGATGCTGCTAAGAATGCAGCTGACATGGTCTTGCTCGATGACAACTTTGCATCTATAGTC
  ACAGGGGTGGAGGAAGGTCGCCTGATCTTCGACAACCTGAAGAAGACCATTGCTTACACCCTGACAAAGA
  ACATCGCTGAGCTCTGCCCTTTTTTGATCTACATTGTTGCTGGGCTCCCCCTGCCTATTGGCACCATCAC
  TATCCTATTCATCGACCTGGGCACAGACATTATCCCCTCCATTGCCTTGGCCTATGAGAAAGCGGAAAGT
  GACATTATGAATAGGAAACCTCGCCACAAGAAGAAAGACAGACTGGTGAACAAGCAGCTGGCCATCTACT
  CTTACCTTCACATTGGTCTCATGCAAGCCCTGGGAGGTTTCCTCGTCTATTTCACTGTCTATGCACAGCA
  GGGCTTTTGGCCCACCTCTCTCATCAACCTGAGGGTATCATGGGAAACAGATGACATAAATGACTTGGAA
  GATAGCTACGGGCAGGAATGGACGAGGTATCAGAGAAAATACCTGGAATGGACAGGCTCAACGGCTTTCT
  TTGTTGCCATCATGGTCCAACAAATTGCAGATCTGATCATCAGGAAAACCCGGAGGAATTCCATCTTCCA
  GCAGGGGCTCTTCAGAAACAAAGTCATCTGGGTGGGGATCATCTCGCAGATCATCGTTGCGTTGGTCCTC
  TCCTACGGTCTCGGGAGTGTCACAGCTCTGAGCTTTACCATGCTCAGGGCTCAGTACTGGTTTGTGGCTG
  TCCCGCATGCCATTTTGATTTGGGTGTATGACGAGATGCGGAAGTTGTTCATCAGGCTCTACCCTGGAAG
  CTGGTGGGACAAGAACATGTATTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_138652
• Insert Size: 3108 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138652.2, NP_619593.2
• RefSeq Size: 3954 bp
• RefSeq ORF: 3108 bp
• Locus ID: 192113
• UniProt ID: Q9Z1W8
• Cytogenetics: 14 C3
• Gene Summary: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.[UniProtKB/Swiss-Prot Function]