Grip1 (NM_133442) Mouse Untagged Clone

CAT#: MC223283

Grip1 (untagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grip1
• Synonyms: 4931400F03Rik; eb; GRIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223283 representing NM_133442
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCTGGAAGAAGAACATCCCCATCTGCTTACAAGCGGAGGAGCAGGAGAGAGAGGAATTCAAGG
  GCTCCACAGTGGTGGAGTTGATGAAGAAGGAGGGAACCACTCTTGGCCTGACGGTATCGGGAGGCATTGA
  TAAAGATGGCAAGCCAAGAGTGTCCAACCTGCGGCAGGGAGGAATCGCTGCCAGAAGTGACCAGCTGGAT
  GTGGGCGACTACATCAAGGCGGTGAATGGGATCAACCTGGCCAAGTTCCGCCACGATGAGATCATCAGCC
  TGCTGAAAAATGTCGGGGAAAGAGTGGTCCTGGAGGTCGAGTACGAGCTTCCACCGGTCTCTGTACAAGG
  ATCCAGTGTTATGTTCCGAACTGTGGAGGTCACGCTGCACAAAGAAGGCAACACCTTTGGTTTTGTCATC
  CGAGGGGGAGCGCATGATGACAGGAACAAGTCCCGTCCGGTTGTGATAACCTGTGTTCGTCCTGGAGGGC
  CTGCTGACAGAGAGGGCACCATCAAACCTGGAGACAGGTTGCTCAGCGTGGATGGAATTCGGCTCCTGGG
  AACCACCCATGCCGAGGCCATGAGCATCCTTAAACAGTGCGGACAAGAAGCCACGCTGCTGATAGAATAC
  GATGTCTCTGTGATGGATTCTGTGGCGACAGCATCCGGGCCACTACTAGTTGAAGTTGCCAAAACTCCAG
  GTGCCAGCCTTGGGGTTGCCCTAACTACCTCCGTGTGCTGTAACAAGCAGGTCATTGTCATAGACAAAAT
  CAAATCTGCAAGCATTGCGGACAGATGTGGCGCGCTACACGTGGGAGACCACATCCTCTCCATTGACGGC
  ACGAGTATGGAGTACTGTACCCTCGCAGAAGCGACCCAGTTCCTGGCCAATACCACTGACCAGGTCAAGC
  TGGAGATTCTCCCACACCATCAGACCCGCCTGGCCCTAAAGGGCCCTGACCATGCGGCTATGGTGCCCTC
  ATCTTCTCCTACCTCCATGAGTGCGTACAGTCTGAGTTCCCTGAACATGGGGACTTTACCTCGAAGCCTC
  TACTCCACCAGCCCACGAGGAACCATGATGAGGAGGAGACTGAAAAAGAAAGACTTCAAAAGCTCACTGT
  CTTTAGCCTCCAGCACTGTGGGGTTGGCTGGCCAGGTCGTTCACACTGAAACCACAGAGGTTGTGCTGAC
  GGCTGACCCTGTCACGGGCTTCGGAATCCAACTGCAGGGCAGCGTGTTTGCCACAGAGACGCTCTCCTCT
  CCGCCTCTGATTTCCTATATTGAAGCTGACAGCCCAGCAGAGAGATGTGGTGTGCTACAGATTGGAGACA
  GAGTCATGGCCATTAATGGAATCCCAACAGAAGACAGCACCTTCGAGGAAGCCAATCAACTCCTGAGAGA
  CTCTTCCATCACGAGCAAAGTCACACTAGAAATCGAGTTTGATGTTGCAGAGTCTGTCATCCCAAGTAGT
  GGAACATTTCATGTAAAACTGCCCAAGAAGCACAGCGTGGAACTTGGAATAACCATCAGTTCGCCATCCA
  GTAGAAAACCGGGGGACCCCCTTGTCATTTCAGATATCAAGAAAGGCAGTGTGGCACACAGAACCGGAAC
  TCTGGAACTGGGAGACAAACTGCTTGCGATAGATAACATCCGGCTGGATAACTGTTCCATGGAAGATGCG
  GTCCAGATCCTCCAGCAGTGTGAAGACCTGGTGAAGCTCAAAATCCGCAAAGATGAAGATAACTCAGACG
  AGCAAGAGAGTTCCGGAGCGATTATTTACACGGTGGAGCTGAAGCGCTATGGGGGGCCCCTTGGCATCAC
  AATTTCTGGAACTGAAGAGCCGTTTGATCCTATTATCATCTCGAGCCTCACTAAAGGGGGATTAGCTGAA
  AGGACTGGAGCGATCCACATCGGAGATAGAATCCTAGCCATCAATAGCAGCAGCTTGAAGGGGAAGCCTC
  TGAGTGAAGCCATCCACTTGCTCCAGATGGCAGGAGAGACTGTCACCCTGAAAATTAAGAAACAGACAGA
  TGCCCAATCTGCATCAAGTCCCAAGAAGTTCCCCATCCCTGGCCACTCGGGGGACCTAGGAGATGGTGAG
  GAGGACCCCTCCCCAATACAGAAACCTGGCAAGCTCTCCGATGCGTACCCCTCCACGGTGCCCAGCGTGG
  ACAGTGCTGTGGACTCCTGGGATGGGTCTGGAATAGATGCCAGCTATGGGAGTCAAGGCTCAACTTTTCA
  GACTTCAGGATACAATTACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGTCCCCAGTC
  CCCAAGCCTCGAAGCCAGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATCCACAGCCA
  GTGGCTTTGTAGGGGCTTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAAGCATTGGA
  GGACCTGGAGACCTGCGGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGGGGAGTACT
  ATGAGTTTGAATCATGAGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCAGGAACGGA
  GCAGTTCACGGCCACACTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGCAGAAAGTC
  TGTAACCCTGCGGAAAATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGCTACACAAG
  GTGACCTTATACAAGGACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCTGGAGAAAG
  GCGTGTATGTCAAAAATATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTACGACAGGCT
  CTTACAGGTAAATCACGTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAGCTGAATCT
  GGCAACAAGCTGGACCTGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACAGCCGGCTC
  TGCCCAGCGACTGGAGCGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAATCTAGAGAC
  ACGAGAACCCACTAACACACTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133442
• Insert Size: 3105 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133442.2, NP_597699.1
• RefSeq Size: 5080 bp
• RefSeq ORF: 3105 bp
• Locus ID: 74053
• UniProt ID: Q925T6
• Cytogenetics: 10 67.33 cM
• Gene Summary: This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (3) contains multiple differences, compared to variant 1, including the lack of multiple exons. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (3, also known as 1b-short) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.