Mdga2 (NM_207010) Mouse Untagged Clone

CAT#: MC223223

Mdga2 (untagged) - Mouse MAM domain containing glycosylphosphatidylinositol anchor 2 (Mdga2), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdga2
• Synonyms: 6720489L24Rik; 9330209L04Rik; Adp; Mamdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223223 representing NM_207010
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTAGTGTACGGTCTCGTGTGGCTGCTGACAGTCCTCCTGGAGGGAATCTCTGGCCAAGGAGTGT
  ACGCTCCCCCGACAGTTCGGATTGTGCACTCAGGATTGGCCTGTAATATCGAGGAGGAGCGCTACTCCGA
  AAGGGTCTATACCATCCGGGAAGGAGAAACTCTAGAATTGACCTGCCTGGTTACTGGACATCCACGCCCA
  CAGATCAGGTGGACCAAAACAGCAGGAAGCGCCTCTGACAGATTCCAAGACTCAAGTGTCTTCAATGAGA
  CTTTGAGGATTACAAACATTCAGAGACACCAAGGAGGCCGATATTACTGTAAAGCAGAGAATGGCTTGGG
  GTCACCAGCAATAAAGTCGATCCGAGTGGATGTGTACTACTTGGATGATCCAGTAGTAACTGTTCATCAA
  AGTATAGGTGAAGCTAAAGAACAATTTTACTATGAGAGAACAGTGTTTCTCCGATGTGTGGCCAATTCCA
  ACCCCCCTGTGCGCTATAGCTGGAGACGTGGTCAGGAGGTATTACTACAAGGATCTGACAAGGGAGTGGA
  GATTTATGAGCCCTTTTTTACCCAGGGGGAAACGAAGATCTTAAAGCTAAAGAATCTTCGCCCTCAAGAT
  TATGCTAACTACAGCTGCATTGCTTCAGTAAGGAACGTGTGTAATATTCCAGACAAGATGGTGTCGTTTA
  GACTTTCCAATAAAACTGCTTCTCCGTCAATTAAGCTCTTGGTGGATGATCCTATAGTTGTAAATCCTGG
  AGAGGCCATAACGTTAGTATGTGTTACAACTGGAGGAGAGCCTACACCCTCTCTTACCTGGGTCAGGTCC
  TTCGGGACTCTGCCTGAAAAGATTGTTTTGAATGGAGGGACATTGACCATACCTGCCATCACCTCTGATG
  ACGCTGGTACTTACAGCTGCATTGCCAATAATAATGTGGGAAACCCTGCAAAAAAGTCCACCAACATCAT
  TGTGAGAGCATTAAAAAAAGGACGATTTTGGATCACTCCTGATCCTTATCACAAAGATGACAACATCCAG
  ATTGGGCGTGAGGTGAAAATATCTTGCCAAGTAGAAGCTGTTCCCTCTGAGGAGTTAACATTCAGTTGGT
  TTAAAAATGGTCGTCCATTAAGAAGTTCTGAACGGATGGTTATTACACAGACTGACCCTGATGTGTCCCC
  CGGAACAACAAACTTGGATATCATTGACTTAAAATTCACAGACTTTGGGACTTACACATGCGTGGCCTCT
  CTGAAGGGAGGAGGAATATCTGATATCAGTATTGATGTTAACATTTCCAGCAGTACAGTTCCACCCAATC
  TGACTGTTCCACAGGAAAAATCACCTTTGGTCACCAGAGAAGGAGACACAATAGAACTTCAGTGTCAAGT
  AACTGGGAAACCAAAACCAATCATCCTTTGGTCTAGAGCAGATAAAGAAGTTGCAATGCCTGATGGGACA
  ATGCAAATGGAAAGTTATGATGGGACCCTGAGGATTGTGAATGTTTCAAGGGAAATGTCAGGAATGTACA
  GATGCCAGACCAGCCAGTACAATGGATTCAACGTGAAGCCAAGAGAAGCCTTGGTGCAGCTCATTGTGCA
  GTATCCCCCAGCTGTGGAACCAGCATTCTTGGAAATCCGTCAAGGACAGGACAGAAGTGTGACCATGAGC
  TGTAGGGTCCTGAGAGCCTATCCAATACGAGTGCTGACCTATGAGTGGCGTCTGGGCAATAAATTATTAC
  GGACCGGACAGTTTGACTCTCAGGAGTATACAGAGTACCCACTAAAGAGTCTTTCCAATGAAAACTATGG
  AGTTTATAACTGCAGCATCATAAATGAAGCTGGAGCAGGGAGATGCAGCTTTCTTGTTACAGGAAAGGCC
  TATGCCCCAGAGTTCTACTATGACACCTACAACCCCGTGTGGCAGAACCGACACCGAGTGTATTCTTACA
  GTCTACAGTGGACACAAATGAACCCTGATGCAGTTGATCGAATTGTTGCATACCGCTTGGGTATTCGGCA
  GGCTGGACAGCAACGCTGGTGGGAACAGGAGATTAAAATAAATGGAAATATTCAGAAAGGAGAACTGATT
  ACATATAACTTGACTGAACTTATTAAACCAGAGGCCTATGAAGTCCGATTGACTCCTCTCACTAAATTTG
  GTGAAGGAGATTCAACAATTCGTGTAATTAAATATACAGGGGAATTTCACTGTGGATTTGAAGATGGTAA
  TATTTGTTTGTTCACCCAAGACGATACGGATAACTTTGACTGGACAAAGCAAAGCACTGCAACAAGGAAC
  ACAAAATACACCCCTAACACGGGTCCCAGTGCAGATCGGAGTGGTTCCAAAGAAGGTTTTTATATGTACA
  TTGAGACATCGCGACCCAGACTAGAAGGCGAAAAGGCTCGACTTCTCAGCCCTGTATTCAGCATAGCTCC
  CAAAAATCCATATGGACCTACAAATAGTGCATATTGTTTCAGTTTCTTCTATCACATGTACGGGCAACAT
  ATAGGGGTTTTAAATGTATATCTACGTTTGAAAGGGCAGACAACGATAGAGAATCCGCTATGGTCGTCGA
  GTGGGAACAAAGGACAACGATGGAATGAAGCTCATGTTAATATATATCCAATTACTTCATTTCAGTTAAT
  TTTTGAAGGCATTCGAGGTCCTGGGATAGAGGGTGACATCGCCATTGATGATGTATCAATTGCTGAAGGA
  GAATGTGCAAAACAAGACCTACCAACTAAGAATTCCGTGGATGGTGCTGTTGGGATCTTAGTTCATATAT
  GGCTTTTTCCAGTTATCATCCTCATCTCTATCTTAAGCCCTCGAAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_207010
• Insert Size: 2850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207010.2, NP_996893.3
• RefSeq Size: 9813 bp
• RefSeq ORF: 2850 bp
• Locus ID: 320772
• UniProt ID: P60755
• Cytogenetics: 12 27.63 cM
• Gene Summary: May be involved in cell-cell interactions.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) lacks an in-frame exon in the coding region, compared to variant A. The resulting protein (isoform B) is shorter, compared to isoform A. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to annotate a downstream in-frame AUG. The downstream AUG is predicted to encode a signal peptide which is expected for this GPI-anchored protein. PMID: 23358245 shows evidence that this protein is processed through the ER and would likely require a signal peptide. The upstream in-frame AUG is likely bypassed by leaky scanning to allow for translation from the downstream AUG.