Parp1 (NM_007415) Mouse Untagged Clone

CAT#: MC223198

Parp1 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Parp1
• Synonyms: 5830444G22Rik; Adprp; Adprt1; AI893648; ARTD1; C80510; PARP; parp-1; PPOL; sPARP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223198 representing NM_007415
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCCTCGGAGAGGCTTTATCGAGTGGAGTACGCGAAGAGCGGGCGCGCCTCTTGCAAGAAAT
  GCAGCGAGAGTATTCCCAAGGACTCCCTCCGCATGGCCATCATGGTGCAGTCACCCATGTTCGATGGGAA
  AGTCCCACACTGGTACCACTTCTCCTGCTTCTGGAAGGTGGGCCACTCCATCCGGCAGCCTGATGTTGAG
  GTGGATGGCTTCTCTGAGCTGCGCTGGGATGATCAGCAGAAGGTCAAGAAGACGGCCGAGGCTGGAGGCG
  TGGCAGGCAAAGGCCAGGATGGAAGTGGCGGCAAGGCGGAGAAGACATTGGGTGACTTTTTAGCGGAGTA
  CGCCAAGTCCAACAGGAGCATGTGCAAGGGCTGCCTGGAGAAGATAGAGAAGGGCCAGATGCGCCTGTCC
  AAGAAGATGGTGGATCCAGAGAAGCCACAGCTGGGTATGATTGACCGCTGGTACCATCCAACTTGCTTTG
  TCAAGAAGCGGGACGAGCTGGGCTTCCGGCCTGAGTACAGTGCCAGTCAGCTCAAGGGCTTTAGCCTCCT
  CTCTGCAGAAGACAAAGAAGCTCTGAAGAAGCAGCTCCCGGCCATCAAGAATGAAGGAAAGAGAAAAGGT
  GACGAGGTGGATGGAACAGATGAAGTGGCCAAAAAGAAATCTAAGAAAGGGAAGGACAAGGATAGTAGTA
  AGCTGGAGAAGGCCCTCAAGGCTCAGAATGAGCTGATCTGGAATATCAAAGACGAGCTGAAGAAAGCGTG
  TTCCACCAACGACCTGAAGGAGCTGCTCATCTTCAACCAGCAGCAGGTGCCGTCAGGAGAGTCAGCGATC
  TTGGACAGAGTTGCTGACGGCATGGCGTTTGGGGCCCTTCTGCCCTGCAAGGAGTGTTCAGGCCAGCTGG
  TCTTTAAGAGCGACGCTTATTACTGTACTGGGGATGTCACTGCCTGGACCAAGTGCATGGTCAAGACACA
  GAATCCTAGCCGAAAGGAATGGGTAACTCCAAAGGAATTCCGAGAAATATCCTACCTCAAGAAGTTAAAG
  GTCAAAAAACAGGACCGAATATTCCCTCCAGAAAGCAGCGCCCCAGCACCACTGGCACTGCCCCTCTCTG
  TCACCTCAGCACCCACAGCTGTGAACTCCTCTGCTCCAGCAGACAAGCCCCTGTCTAACATGAAGATCCT
  GACTCTTGGGAAGCTCTCCCAGAACAAGGACGAAGCAAAAGCTGTGATTGAGAAACTCGGAGGCAAGTTG
  ACAGGATCTGCCAACAAGGCCTCCTTGTGTATCAGCACTAAAAAGGAGGTGGAGAAGATGAGTAAGAAGA
  TGGAGGAAGTGAAAGCGGCCAACGTTCGAGTTGTGTGTGAGGACTTCCTCCAGGACGTGTCTGCCTCCAC
  TAAAAGCCTCCAAGAGCTGCTCTCGGCCCACAGCTTGTCCTCGTGGGGGGCTGAGGTGAAGGCAGAGCCT
  GGTGAAGTGGTGGCCCCCAAGGGGAAGTCAGCTGCACCCTCCAAGAAGAGCAAGGGTGCTGTCAAGGAGG
  AAGGTGTCAACAAATCTGAAAAGAGGATGAAATTAACTCTGAAGGGAGGAGCAGCCGTTGATCCTGACTC
  TGGTCTGGAACACTCTGCACACGTCCTGGAGAAAGGTGGGAAGGTGTTCAGCGCCACACTTGGCCTGGTG
  GACATTGTGAAAGGGACGAACTCCTATTACAAACTGCAGCTTCTGGAGGACGACAAGGAGAGCAGGTACT
  GGATCTTCCGGTCCTGGGGCCGGGTGGGCACAGTTATCGGCAGTAACAAACTTGAGCAGATGCCCTCCAA
  AGAGGACGCTGTTGAGCACTTCATGAAGCTGTATGAAGAGAAGACTGGGAATGCCTGGCACTCGAAAAAC
  TTCACAAAGTATCCCAAGAAGTTCTACCCTCTGGAGATTGACTATGGCCAGGACGAAGAGGCAGTAAAGA
  AGCTGACGGTGAAGCCTGGCACCAAGTCGAAGCTGCCGAAGCCAGTGCAGGAGCTCGTGGGGATGATCTT
  CGACGTGGAGAGCATGAAAAAGGCCTTGGTGGAGTACGAGATTGACCTCCAGAAGATGCCCTTGGGGAAG
  CTGAGCAGAAGGCAGATCCAGGCCGCCTACTCTATCCTCAGCGAGGTCCAGCAGGCAGTGTCTCAAGGCA
  GCAGTGAATCCCAGATCCTAGATCTCTCCAATCGCTTCTACACTCTGATCCCCCATGACTTTGGAATGAA
  GAAGCCCCCACTCCTGAACAACGCAGACAGCGTGCAGGCCAAGGTGGAGATGCTAGACAACCTCCTGGAC
  ATCGAGGTGGCCTATAGTCTTCTCAGGGGTGGCTCTGACGACAGCAGCAAGGATCCCATCGACGTCAACT
  ACGAGAAACTCAAAACTGACATTAAGGTGGTTGACAGAGATTCTGAAGAGGCCGAGGTCATCAGGAAGTA
  CGTGAAGAACACTCATGCTACCACGCACAACGCCTATGACCTGGAAGTGATCGATATCTTCAAGATAGAG
  CGCGAGGGGGAGAGCCAGCGCTACAAGCCCTTCAGGCAGCTTCACAACCGGAGGCTGCTGTGGCACGGCT
  CCAGGACCACCAACTTTGCTGGCATCCTGTCGCAGGGTCTGCGGATAGCCCCACCTGAAGCGCCTGTGAC
  AGGCTACATGTTTGGGAAAGGGATCTACTTTGCCGACATGGTGTCCAAAAGTGCAAACTACTGCCACACA
  TCTCAGGGAGACCCGATTGGCTTAATACTGCTGGGAGAGGTTGCCCTTGGAAACATGTATGAACTCAAGC
  ATGCTTCACATATCAGCAAGTTACCCAAGGGCAAGCACAGTGTCAAAGGTTTGGGAAAAACCACCCCTGA
  CCCTTCGGCCAGCATCACCCTGGAGGGTGTAGAGGTTCCACTGGGAACAGGGATCCCATCTGGTGTCAAC
  GACACCTGCCTGCTGTATAATGAGTACATTGTCTACGACATTGCTCAGGTGAATCTCAAATACCTGCTGA
  AACTCAAGTTCAATTTTAAGACATCCCTGTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007415
• Insert Size: 3045 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012041, AAH12041
• RefSeq Size: 3845 bp
• RefSeq ORF: 3045 bp
• Locus ID: 11545
• Cytogenetics: 1 84.44 cM