Dhx36 (NM_028136) Mouse Untagged Clone

CAT#: MC223152

Dhx36 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 36 (Dhx36), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dhx36
• Synonyms: 2810407E23Rik; AI452301; AU022184; Ddx36; mKIAA1488
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223152 representing NM_028136
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACGACTATCATCAGAGCTGGAGCCGCGATGGGGGCCCACGGGGCTCCGGCCAGGGCTCCAGCG
  GCGGCGGCGGCGGAGGGAGCCGGGGCTCGGGCGGCGGCGGAGGGGGCCGCGGCGGCCGGGGCCGGCATCC
  CGCACACCTTAAGGGTCGCGAGATCGGCCTGTGGTACGCCAAGAAGCAGACGCAGAAGAACAAGGAGGCT
  GAGAGGCAGGAGAGAGCTGTAGTGCACATGGATGAACGTCGAGAAGAGCAAATTGTGCAGCTGCTGAATT
  CAGTCCAAGCTAAGACTGACAAAGATTCAGAAGCGCAGATATCCTGGTTTGCTCCTGAGGATCATGGGTA
  TGGTACTGAAGTTTCTTCAGAGAAAAAAATAAACTCAGAGAAGAAACTTGACAACCAGGAAAAGAAATTG
  CTAAACCAAGAAAAAAAGACATTTAGGATCACAGACAAATCATATATTGACCGAGATTCTGAGTATTTAT
  TGCAAGAAAATGAGCCAAACCTAAGCTTAGATCAACATTTACTGGAAGATTTACAAAGGAAAAAAACTGA
  CCCTCGATATATAGAGATGCAGCGTTTCAGAAAAAAGCTGCCTTCATATGGAATGCAGAAGGAGCTGGTA
  AATCTAATCAATAACCATCAGGTGACAGTAATAAGTGGTGAAACTGGTTGTGGCAAAACCACTCAGGTTA
  CGCAGTTCATCTTGGATAACTACATCGAAAGAGGAAAAGGGTCTGCCTGCAGAATAGTGTGTACTCAGCC
  GAGAAGAATTAGTGCCATTTCAGTCGCTGAGAGAGTGGCCACAGAAAGGGCAGAGTCTTGTGGCAATGGT
  AATAGTACTGGATACCAGATTCGTCTTCAAAGTCGGTTGCCAAGGAAACAAGGTTCTATCTTATACTGCA
  CAACAGGAATCATTCTTCAGTGGCTCCAGTCCGACTCACGTTTGTCCAGTGTTAGTCATATTGTACTTGA
  TGAAATTCATGAAAGGAATCTACAGTCGGATGTTTTAATGACTGTTATTAAAGATCTTCTCCATTTTCGA
  TCTGATCTCAAAGTAATATTGATGAGTGCAACTTTGAATGCTGAGAAATTTTCAGAATATTTTGGTAACT
  GTCCAATGATACATATACCCGGGTTTACTTTTCCAGTTGTGGAATATCTTTTGGAAGATATCATTGAAAA
  AATAAGATATGTTCCAGACCAAAAAGAACATAGATCCCAGTTCAAGAGGGGTTTCATGCAGGGTCATGTA
  AATAGACAAGAAAAAGAAGAAAAAGAGGCCATCTATAAGGAACGCTGGCCAGCGTATATAAAGGAACTGC
  GGACAAGATACTCTGCAAGTACCGTAGATGTTTTGCAAATGATGGATGATGATAAAGTTGATCTGAATTT
  GATTGCTGCCCTTATTCGATACATTGTTTTGGAAGAAGAGGATGGTGCAATATTGGTCTTTCTACCAGGC
  TGGGACAATATCAGTACTTTGCATGATCTCTTGATGTCACAAGTGATGTTTAAATCAGATAAGTTTCTCA
  TTATACCTTTACATTCACTGATGCCTACCGTCAACCAGACACAGGTATTTAAAAAAACTCCTCCCGGTGT
  TCGGAAAATAGTAATTGCTACCAACATTGCAGAGACTAGCATCACCATAGATGATGTGGTTTATGTAATA
  GATGGAGGAAAAATTAAAGAAACACACTTTGACACACAGAACAACATCAGTACCATGTCTGCTGAGTGGG
  TTAGTAAAGCTAATGCCAAACAGAGGAAAGGGAGAGCTGGAAGAGTTCAGCCTGGTCATTGTTATCATCT
  GTATAATGGTCTTAGAGCAAGTCTTCTAGATGACTACCAACTACCAGAAATTTTGAGAACTCCTTTGGAA
  GAACTCTGTTTGCAAATAAAGATCTTGAGGCTGGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGATC
  CACCATCAAATGAGGCAGTGGTGCTCTCCATAAAACACCTAATGGAACTGAGTGCTTTGGATAAGCAAGA
  AGAATTAACACCTCTTGGTGTCCATTTAGCCCGACTGCCTGTTGAGCCACATATTGGAAAAATGATTCTC
  TTTGGAGCTTTGTTCTGCTGCTTAGATCCAGTTCTCACCATTGCTGCCAGTCTCAGCTTTAAAGATCCCT
  TTGTCATTCCATTGGGAAAAGAAAAGATTGCAGATGCAAGAAGAAAAGAACTGGCAAAGGAAACTAGAAG
  TGATCACCTGACAGTTGTGAATGCATTTGAGGGCTGGGAAGAAGCCAAACGACGTGGTTTCAGGTATGAA
  AAGGACTACTGCTGGGAATATTTTCTGTCTTCGAACACACTACAGATGCTGCATAACATGAAGGGACAGT
  TTGCTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGTAGAAGTCCCAAAGATCCAAAAGCTAATATAAA
  TTCAGATAATGAGAAGATAATTAAAGCTGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATCCGA
  CTAAATTTGGGTAAAAAGAGGAAAATGGTAAAAGTTCACACGAAGTCTGACGGCCTGGTTTCTATTCATC
  CTAAGTCTGTCAACGTGGAGCAGACAGACTTCCACTACAACTGGCTTATCTATCACCTGAAGATGAGAAC
  AAGCAGTATATACTTGTACGACTGTACAGAAGTGTCACCATACTGCCTCCTGTTCTTTGGAGGAGATATT
  TCCATCCAGAAAGATAAGGATCAGGAAATTATTGCTGTAGATGAGTGGATTGTGTTTCAGTCTCCAGAAA
  GAATTGCCCATCTTGTTAAGGGACTAAGAAAGGAACTGGATAGCCTTCTACAAGAGAAGATTGAAAGCCC
  TCATCCTGTAGACTGGGACGACACTAAGTCAAGAGACTGTGCAGTACTGTCAGCTATTCTAGACTTGATC
  AAAACTCAAGAAAAGGCTACTCCAAGGAACTTGCCACCACGGTCACAGGACGGATATTATAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028136
• Insert Size: 3006 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028136.2, NP_082412.2
• RefSeq Size: 4975 bp
• RefSeq ORF: 3006 bp
• Locus ID: 72162
• UniProt ID: Q8VHK9
• Cytogenetics: 3 E1
• Gene Summary: Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:25611385). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:21703541, PubMed:21590736). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA) (By similarity). Plays a role in genomic integrity (By similarity). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (By similarity). Plays a role in transcriptional regulation. Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:25611385) (By similarity). Plays a role in post-transcriptional regulation (By similarity). Unwinds a G4-RNA structure located in the 3' UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (By similarity). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (By similarity). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3' UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (By similarity). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3' UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (By similarity). Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (By similarity). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541). Required for the early embryonic development and hematopoiesis (PubMed:22422825). Involved in the regulation of cardioblast differentiation and proliferation during heart development (PubMed:26489465). Involved in spermatogonia differentiation (PubMed:25611385). May play a role in ossification (PubMed:21590736).[UniProtKB/Swiss-Prot Function]