Heatr5b (BC086652) Mouse Untagged Clone

CAT#: MC223140

Heatr5b (untagged) - Mouse HEAT repeat containing 5B (cDNA clone MGC:77977 IMAGE:6856641), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Heatr5b
• Synonyms: 2010013B10Rik; A230048G03Rik; AV071430; D330050P16Rik; mKIAA1414
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC086652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTAGCTCACAGTTTATTGCTAAATGAGGAAGCTTTGGCTCAGATCACGGAGGCAAAGAGACCCG
  TGTTCATCTTTGAATGGTTGAGATTTCTTGATAAAGTCCTAGTTGCTGCCAACAAGACAGATGTAAAAGA
  AAAACAGAAAAAACTTGTTGAACAATTAACTGGATTAATAAGCAGCTCACCTGGACCACCTACTCGAAAA
  TTGTTAGCTAAAAACCTTGCAGCCCTTTATAGCATTGGGGATACTTACACAGTTTTTCAAACACTAGATA
  AATGTAATGACATTATCAGAAGTAAGGACGACACTGCAGCCTACTTACCAACAAAATTGGCAGCAGTGGC
  TTGTGTTGGAGCCTTCTATGAGAAGATGGGAAGGATGTTGGGCAGTGCATTTCCAGAAACAGTGAACAAT
  CTGTTGAAATCTCTGAAAAGTGCAGAGTCTCAAGGGCGAAGTGAGATCTTAATGAGTCTGCAGAAAGTGC
  TGACTGGACTGGGCGGCGCAGCAGCTTCCTCTCACCGCGACATTTACAAGAATGCTCGATCCCTCCTGAC
  CGACAGGTCGATGGCTGTCCGATGTGCAGTGGCCAAGTGCCTTCTGGAACTACAGAATGAAGCTGTGTTC
  ATGTGGACTGCCGAACTAGAAAATGTAGCTACCCTCTGCTTTAAGGCTCTGGAAAACTCAAATTATGGGG
  TACGAGTCGCAGTATCTAAGCTACTAGGAACAGTCATGGCTACAGCATTGATGCCGAAACAGGCAACAGT
  CATGCGGCAAAATGTGAAGCGAGCAACCTTCGATGAAGTCTTAGAACTCATGGCCACGGGATTTCTGCGC
  GGAGGGTCGGGTTTCCTAAAGAGTGGCGGAGAAATGTTGAAAGTTGGAGGGTCTGTTAATCGGGAAGTGA
  GAGTTGGTGTGACACAGGCCTATGTTGTCTTTGTGACAACTCTGGGTGGCCAGTGGCTGGAGCGTAGCTT
  TGCCACATTCTTGTCCCACGTACTTGATCTGGTTTCCCATCCTCGAGCAACACAAACACACGTGGACGCT
  GTGTACTCCAGGCGATGTGTGTCCTTCATGCTGAGAGCTACCGTGGGCAGTCTGTTAGGTGAGAAAGCCC
  AGATAGCAGCTGCCAAGGAGATCTGCCAGGCAATCGGAAAGCAGATGAAAGCTGTGGAAGCAGTAGTGAA
  TGATACGAGCAGTGAAAACAAATCGGGGACAGCAGACATTGCAGCCAGCCAGCACGTCATGGTCTGTGCC
  CTGCAGGAGCTTGGGAGCCTGGTGCAGAGCCTGAATGCCACCGCCTCTCCTCTTATTCAGGAAGCGTCCA
  TAGGACTTTTGGAGATTGTGACGTCAGTGCTTCTTCACCCAAGCATGGCTGCTCGGCTTGCTGCTGCCTG
  GTGTCTGCGTTGTGTAGCTGTGGCGCTGCCCTTCCAGTTGACACCATTTCTGGATAGGTGTGCAGAACGG
  CTCAACAACCTCAAGACATCCCCAGAAGCTGTTAGTGGATACAGTTTTGCAATGGCCGCCTTGTTGGGTG
  GAGTGCATCAGTGTCCTTTGGGCATTCCTCATGCCAAAGGAAAGATGGTGGTCAGCATTGCTGAAGATCT
  GCTACGAACTGCTGCCCAAAACAGCAGGCTGTCCCTGCAGCGCACCCAGGCTGGCTGGCTTCTTCTGGGA
  GCCCTCATGACTTTAGGACCGTCTGTTGTTCGTTACCATCTACCCAAGATGTTGCTGCTGTGGCGGAACG
  TGTTCCCTCGCTCTTTAAAGGAACTGGAGGCTGAGAAGGCCCGGGGGGATTCATTCACCTGGCAGGTCAC
  TCTGGAAGGTCGCGCTGGGGCTCTGTGTGCCATGAGGAGTTTTGTTGCACATTGTCCTGAGCTGCTAACA
  GAGGATGCAATTAGGAAATTAATGACTCCTATCGAATGTGCCATGACCATGATGTCGCACATTCCATCCG
  TCATCAAGGCCCATGGAGCCCATCTGAAAGCTAGTGCCGCAATGGTCCGCTTAAGACTTTATGATATCTT
  GGCTTTATTACCTCCAAAAACTTATGAAGGGTCTTTCAATGCACTTCTTAGAGAGCTGGTGGCAGAGTTC
  ACTCTGACTGACAACTCGGCCAACACAACTACCTCCCTCCTTCGCTCCCTCTGCCACTATGACGACAGCG
  TTCTCCTGGGCTCCTGGCTTCAAGAGACTGATCATAAATCCATTGAAGACCAGCTCCAGCCAAACAGTGC
  ATCTGGCAGTGGGGCTCTGGAACACGACCCTTCTTCCATTTACCTACGGATACCTGCAGGTGAAGCCGTG
  CCGGGTCCGCTCCCTCTCGGAGTCTCAGTGATTGACGCATCTGTGGCTCTGTTCGGGGTTGTGTTTCCAC
  ATGTTTCCTATAAACACAGGTTACAAATGCTGGATCACTTTGCTGAATGTGTTAAGCAAGCCAAAGGTGT
  CCGCCAGCAGGCTGTGCAGCTTAATATATTTACTGCTGTTCTTAGTGCACTGAAGGGCTTAGCTGAAAAC
  AAGAGTACTTTGGGACCTGAGGAAGTCCGCAAGTCTGCTCTGACACTGGTTATGGGAGCCCTCGACAACC
  CAAACCCCATCTTACGGTGTGCGGCAGGGGAAGCCCTTGGAAGAATGGCTCAGGTGGTGGGAGAAGCCTC
  TTTCATTGCCCGAATGGCCCAATACAGCTTTGACAAGTTGAAGTCAGCTCGAGATGTCGTGTCCAGGACT
  GGTCACTCACTGGCCCTCGGTTGTTTACATCGTTATGTTGGTGGCATAGGATCAGGACAACACCTAAAGA
  CCAGTGTCAGCATTTTACTGGCTCTAGCTCAAGATGGGACATCCCCTGAAGTTCAGACGTGGTCTCTTCA
  TTCACTCGCCCTGATAGTGGATTCCAGCGGCCCAATGTACCGTGGGTACGTGGAGCCCACGTTGTCCCTG
  GTACTCACCTTGCTGCTGACAGTTCCACCTTCACACACAGAAGTTCACCAGTGCCTGGGCAGATGCTTGG
  GGGCGATCATAACTACTGTTGGACCAGAGCTGCAGGGAAATGCAGCGACCATATCCACCATCCGCTCCTC
  CTGCTTGGTGGGCTGTGCCATTACCCAGGACCACTCTGACTCCCTTGTTCAGGCAGCTGCCATCTCTTGC
  CTTCAGCAGCTTCACATGTTTGCCCCCACCACACATTCCACACCGACAATTAAGCTGAAAACAAGCTTTT
  TTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC086652
• Insert Size: 3225 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC086652, AAH86652
• RefSeq Size: 3463 bp
• RefSeq ORF: 3224 bp
• Locus ID: 320473
• Cytogenetics: 17 E3
• Gene Summary: Component of clathrin-coated vesicles (By similarity). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (By similarity).[UniProtKB/Swiss-Prot Function]