Map3k21 (NM_145608) Mouse Untagged Clone

CAT#: MC223133

BC021891 (untagged) - Mouse cDNA sequence BC021891 (BC021891), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k21
• Synonyms: mKIAA1804; Mlk4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223133 representing NM_145608
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTACCTGTAGCCGAGGGCACGGCTGACACGCCGCTGTCCCCGGCCCGGGACGACTCGGGGAGTA
  CCTCCTCCGGGATGTGGGCCGCGCTTTATGATTACGAAGCGCGCGGTGAGGACGAGCTGAGCCTGAGGCG
  CGGTCAGCTGGTCGAGGTACTGTCGCAGGACGCCGCGGTGTCAGGAGACGAGGGCTGGTGGGCCGGCCAA
  GTGCAGCGGCGCCTGGGCATCTTTCCGGCCAGCTACGTGGCGCCCTGTGGCCCGGTGCCGCCGCCGGCGC
  CGCCCCCGCCGAGACCCTGCTCGCCTGTGCACGTAGACTTCGAGCGCCTGGAGCTCAAGGAGCTGATCGG
  CGCCGGCGGCTTCGGTCAGGTGTACCGCGCCACCTGGCAGGGCCAGGAGGTGGCGGTGAAGGCGGCGCGC
  CGGGACCCGGAGCAGGACGCGGCGGCGGCGGCCGAGAGCGTACGGCGCGAGGCGCGGCTCTTCGCTATGC
  TGCGGCACCCCAACATCATCCAGCTGCGGGGTGTGTGCCTGCGGCAGCCGCACCTCTGCCTGGTGCTGGA
  GTTCGCGCGTGGCGGCGCGCTCAACCGCGCGCTGGCGGCGGCAGCATCGGATCCGCGAGCGCCCGGCCCC
  CGCCGCGCGCGCCGCATCCCCCCACAGGTTCTGGTCAACTGGGCGGTGCAGATCGCCCGCGGCATGCTGT
  ACCTGCACGAGGAGGCGGTGGTGCCCATCCTGCACCGTGACCTCAAGTCCAGCAACATTTTGCTGCTGGA
  GAAGATAGAACATGATGACATTTGCAACAAGACCCTGAAGATCACGGACTTTGGGCTAGCGAGGGAATGG
  CACAGGACCACCAGGATGAGCGCAGCAGGGACCTACGCCTGGATGGCTCCCGAGGTCATCAGGTCCTCCC
  TGTTCTCCAAGGGCAGCGACATCTGGAGCTATGGAGTGCTGTTGTGGGAGCTGCTCACTGGTGAGGTCCC
  GTACCGTGGCATCGATGGACTGGCTGTGGCGTACGGGGTGGCTGTCAACAAGCTCACACTGCCTATTCCA
  TCCACCTGCCCTGAACCGTTTGCAAAACTCATGAAAGAGTGCTGGGAACAAGACCCTCATATCCGCCCCT
  CGTTTGCCTTAATTCTTCAGCAGCTGACTGCGATCGAAGAGGCAGTGTTGACCAACATGCCCCAGGAGTC
  CTTCCATTCCATGCAGGAAGACTGGAAACTGGAGATTCAGCAAATGTTCAGTGAGCTGCGAACCAAGGAG
  AAGGAGCTGCGTTCCCGAGAGGAGGAGCTGAGCCGAGCTGCCTTGCAACAGAAATCGCAGGAACTGCTGC
  TCAGACGCCGCGAGCAGCAGCTCGCGGAGCGTGAGATCGACGTGCTGGAGCGCGAGCTGAACGTGCTCAT
  CTTCCAGCTGAGCCAGGAGGCTCCCCACGTGAAGAAGAGGAAGGGAAGGTTCAGGAGAGGACGGCTGAGG
  CTCAAAGACGGCCACCGCATCAGCCTGCCTTCAGATTTCCAGCACAAGATCACAGTGCAGGCTTCCCCGA
  CGCTGGACAAGCGCCGAAGCTCAGACAGCGGACTGTGCAGCCCACCCGGCAGCCCCTTGATGCTCCCCCG
  CCTCCGGGCTATTCAGTTAACTTCAGATGAGAACAATAAAACCCGGGGAAGGAACATGGTCTTCCGCCAA
  GAAGACTTTGAAGACGTGAAAAGGAGTTTCAAGAAAAAAGGATGTACGTGGGGACCAAGCTCCGTTCAAA
  CAAAGGAAAGACCAGAAGGCAGAGAGAGGGTCAGACCTCTCTCAGATGGCAACAGCCCTTGGTCATCTCT
  TTTAATAAAAAGCCAGAAAACCACACCCTTGGCTTCACTGTTTGTGGACCAGCCAGGTTCTTGTGAGGAG
  CAGAAACTTGTCCCTGAAGGATTAGAACATAGAAAACCAAAGCAAACGAAGTTCCCTGGCCAGGCCCACG
  TTGGTCTACCTCTTTGCAAAGACAGCCAGAGAGAAGACTCATCAGAGGCTGAGAGTCGGGAGGAGGGATC
  CCCTAAGGGGTCCCCGGTGAACAACGTGGGTGCACCCATGCTGAGGAAGAAAACGGAGTCAGCGCTGTGT
  GAGTGTGGGATGCTGCTGGCATCGATGGCTTTGGGACTGGATGTCAGGAAACTTCACGGAGCCCAGGCCC
  CCGCAAAACCATCACCCAAGATGGAAAAGAAGGAAGAGGGTGCCCTCCAACCTGCCTCCAGGTGCCAATC
  AAGTCCCAGTTCCCTTCTGAGGCAGCCATCTGCAGGCAGGGCACCCAGCGGTGGCTCCACGCTCCTGCTG
  CCCAGTGCCCCAAGCCATTCCTCAAAGTCTTCTCTCTCCATGAAGTGTTTGCTGCAGGCTGGTAAGGAGG
  AGTCATCTTTGGGGAATGCTCGGGACTTGTGTGGTCCTACGACGCTCACTCCAGATCCTGGCTCTGCCGC
  ACCAGAGAGCGGCTGTGAACTCATCCCCGGACTGAGACCTAAGACTGACTATGGTGTGTTGAGAAGCATG
  CCGCATGCCATCCTGGAGCAGACAGGGGAGAGACTGCCAGGCTGTGCCATTGTAGGAGACAAAGGATGCC
  ACCACATGCAGATGGGATCTGAAGAAACTCCTCTCTGGCTCCAAAGTGCCCCTGAAGACTCTGGATTACC
  ACACTCTCCCTCCCCTGGACCTCAAAGGGACCTGGCCAGCCAGGCCTCTCTTGTGAAACCTGAAGGTGTT
  CTGGGCGAGTGCCAGGCATGCCCGGCTCTGCCACAAAGGCCACACACTGCTTCGGTGAGAACAACATCTC
  CACCTACCTGGGTTTGTGACAAGGACCATCAGGTGCCAGCCCTTGCCTGCTTGCTTGGTGCTCAGGAGAG
  AAGCAGGTGTCAGACTCCTTCCTTGCTGGATGCCAGCATAGAAGGTCAGAAGAAGGACTGTGCCATGCCC
  TTGTGTAGAGTGAAGAGCGTGATGTGTCAGCCGTCCATCTACGCCCTGGAGAAAGACTTTCTGACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145608
• Insert Size: 3009 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145608.2, NP_663583.2
• RefSeq Size: 5692 bp
• RefSeq ORF: 3009 bp
• Locus ID: 234878
• UniProt ID: Q8VDG6
• Cytogenetics: 8 E2
• Gene Summary: Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways (By similarity).[UniProtKB/Swiss-Prot Function]