Sympk (BC049852) Mouse Untagged Clone

CAT#: MC223093

Sympk (untagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sympk
• Synonyms: SPK, SYM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC049852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCAGCAGCGGGGACAGTGTCACCCGCCGGAGTGTGGCATCACAGTTTTTCACACAAGAAGAAG
  GGCCAAGCATCGATGGCATGACCACTTCTGAGAGGGTGGTAGATCTTCTGAACCAGGCTGCATTGATCAC
  CAATGACTCCAAGATCACAGTGCTCAAACAGGTCCAAGAGCTGATCATCAACAAGGACCCCACCCTGCTT
  GACAACTTCCTGGATGAGATCATCGCTTTCCAAGCAGACAAATCCATTGAAGTGCGCAAGTTTGTCATTG
  GCTTCATTGAGGAGGCATGCAAGCGGGACATTGAGCTGCTGCTAAAACTGATTGCCAACCTCAACATGCT
  ATTGCGGGATGAAAATGTGAACGTGGTTAAGAAGGCCATCCTCACCATGACCCAGCTCTACAAGGTGGCC
  CTACAGTGGATGGTGAAGTCTCGAGTCATCAGTGATCTCCAGGAGGCCTGCTGGGACATGGTGTCCTCCA
  TGGCTGGGGAAATCATCCTGCTTTTGGATTCTGACAACGATGGCATCCGTACACATGCCATCAAGTTTGT
  GGAGGGCCTCATTGTCACCCTGTCACCCCGCATGGCTGACTCAGAGGTCCCACGACGCCAGGAGCATGAC
  ATCAGTCTGGACCGCATTCCTCGAGATCACCCCTACATCCAGTACAATGTCCTATGGGAAGAAGGCAAGG
  CAGCTGTGGAGCAGCTGCTGAAGTTCATGGTGCACCCCGCCATCTCCTCCATCAACCTGACCACAGCGCT
  CGGCTCCCTTGCCAACATCGCCCGCCAGAGACCCATGTTCATGTCCGAGGTGATCCAGGCCTATGAGACT
  CTGCATGCCAACCTGCCCCCAACGCTGGCCAAGTCCCAGGTGAGCAGTGTGCGGAAGAACCTTAAGCTGC
  ACTTGCTCAGTGTGCTCAAGCACCCTGCCTCCTTGGAGTTCCAGGCCCAGATCACCACCCTGCTGGTGGA
  TCTGGGCACGCCCCAGGCTGAGATCGCCCGAAACATGCCTAGCAGCAAGGACTCCCGCAAGCGGCCCCGG
  GACGATACAGATTCCACGCTCAAGAAGATGAAGCTGGAGCCCAACTTGGGAGAAGACGATGAGGACAAAG
  ACTTGGAGCCTGGCCCATCAGGGACGTCAAAGGCGTCAGCCCAGATCTCAGGCCAGTCAGACACGGACAT
  CACTGCTGAGTTCTTGCAGCCTCTGCTGACACCTGACAATGTGGCCAATCTGGTCCTCATCAGCATGGTG
  TACCTGCCTGAGACCATGCCTGCCTCCTTCCAAGCCATCTACACCCCTGTGGAGTCAGCAGGCACTGAAG
  CCCAGATCAAGCACCTGGCTCGACTCATGGCCACACAGATGACAGCTGCAGGACTGGGTCCTGGTGTGGA
  ACAGACGAAACAGTGCAAGGAGGAGCCCAAGGAGGAGAAGGTGGTAAAGCCGGAGAGCGTCCTGATCAAG
  CGGCGCCTGTCGGTGCAGGGCCAGGCCATCTCTGTGGTAGGCTCTCAGAGCACCATGTCTCCCTTGGAGG
  AGGAGGTACCCCAGGCCAAGAGGAGGCCAGAACCCATCATCCCTGTCACGCAGCCACGGCTGGCAGGCGC
  AGGTGGGCGCAAGAAGATCTTCCGTCTGAGCGATGTGCTGAAGCCCCTGACAGATGCCCAGGTGGAAGCC
  ATGAAGCTAGGAGCTGTGAAGCGGATCCTGAGGGCTGAGAAGGCTGTAGCCTGCAGTGGGGCAGCCCAGG
  TGCGCATCAAGATCCTGGCCAGCCTCGTAACACAGTTCGACTCCGGATTCAAGGCAGAGGTGCTGTCCTT
  TATCCTGGAAGATGTCCGGGCCCGTCTGGACCTGGCTTTTGCCTGGCTCTACCAGGAATATAATGCCTAC
  CTGGCAGCAGGGACCTCAGGCACTCTGGACAAATATGAGGATTGCCTCATCTGCCTGCTCTCTGGACTAC
  AAGAGAAACCAGACCAGAAAGATGGGATCTTTACCAAGGTGGTTTTGGAGGCCCCACTGATCACTGAGAG
  TGCCTTGGAGGTGATACGGAAGTACTGTGAGGATGAGAGTCGTGCCTACCTGGGCATGTCGACACTTGGA
  GACCTGATCTTCAAGCGCCCTTCTCGCCAGTTCCAGTATCTGCACGTTCTTCTTGACCTCAGCTCTCACG
  AGAAGGACAGGGTGCGCTCCCAGGCCTTGCTCTTCATTAAGCGAATGTATGAGAAGGAGCAGCTTCGAGA
  GTATGTGGAGAAATTTGCCCTCAACTACCTGCAGCTCCTGGTCCACCCCAACCCACCCTCGGTGCTGTTT
  GGAGCCGACAAGGACACAGAGGTGGCCGCACCCTGGACAGAGGAAACAGTGAAGCAGTGTTTGTATCTGT
  ATCTGGCTCTCCTGCCTCAGAACCACAAGCTGATCCATGAGCTGGCAGCCGTGTACACTGAAGCCATCGC
  TGACATCAAGCGGACAGTGCTGAGGGTCATTGAGCAGCCGATCCGAGGGATGGGCATGAACTCTCCAGAG
  CTGCTGCTGCTGGTAGAAAACTGTCCCAAGGGGGCAGAGACGCTGGTCACCCGCTGCCTGCACAGCCTCA
  CCGACAAAGTGCCACCATCCCCAGAACTGGTGAAGCGGGTCCGAGACCTGTACCACAAACGGCTGCCTGA
  TGTGCGCTTCCTCATCCCCGTGCTCAATGGGTTGGAGAAGAAAGAGGTGATCCAGGCGCTGCCCAAGCTC
  ATCAAACTCAACCCCATTGTGGTGAAGGAAGTCTTCAACCGCCTGCTAGGCACTCAGCACGGTGAGGGGA
  ACTCGGCCCTGTCCCCCCTGAATCCAGGAGAGCTGCTGATTGCATTGCACAATATTGACTCTGTGAAGTG
  TGACATGAAGTCCATCATCAAAGCCACCAACCTGTGTTTTGCGGAGCGGAATGTGTACACGTCGGAAGTG
  CTAGCTGTGGTGATGCAGCAGCTGATGGAGCAAAGCCCTCTGCCCATGCTGCTCATGCGGACCGTCATCC
  AGTCCCTCACCATGTACCCCCGCCTGGGGGGCTTCGTCATGAACATCCTGGCCCGACTCATCATGAAGCA
  GGTATGGAAGTACCCCAAGGTATGGGAGGGCTTCATCAAGTGCTGCCAGCGCACCAAGCCACAGAGTTTC
  CAGGTCATCCTGCAGCTACCTCCACAGCAGCTTGGCGCAGTCTTTGACAAGTGCCCTGAGCTCCGGGAGC
  CACTGTTGGCCCATGTGCGCTCCTTCACTCCCCACCAGCAAGCACACATCCCCAACTCCATCATGACCAT
  CCTGGAGGCCACTGGAAAGCAGGAGCCCGAAGTCAAGGAAGCACCTTCAGGGCCCCTGGAAGAGGATGAC
  CTGGAGCCCTTGGCTCTGGCTTTGGCCCCGGCTCCGGCCCCGGCCCCGGCCCCGGCCCCTGCCCCACGGC
  CCCCTCAGGACCTCATCGGCCTGCGATTGGCTCAGGAGAAGGCCCTGAAGAGGCAGCTGGAGGAGGAACA
  GAAGCAGAAGCCCACAGGCATCGGAGCGCCCGCTGCCTGTGTGTCCTCAACGCCCTCGGTGCCAGCAGCA
  GCCCGAGCTGGGCCCACCCCAGCTGAGGAGGTCATGGAATACCGGGAGGAGGGTCCCGAATGCGAGACCC
  CGGCCATCTTCATCAGCATGGACGACGACTCGGGGCTGGCTGAAACCACACTGCTGGACTCTAGTCTCGA
  GGGGCCCCTGCCTAAGGAGGCAGCAGCAGTCGGGTCGAGCTCCAAGGATGAGCGGAGCCCCCAGAACCTC
  AGCCATGCTGTGGAAGAAGCCTTGAAGACCTCCAGCCCAGAAACCAGGGAACCCGAGAGCAAGGGGAACA
  GCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC049852
• Insert Size: 3855 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049852, AAH49852
• RefSeq Size: 4071 bp
• RefSeq ORF: 3854 bp
• Locus ID: 68188
• Cytogenetics: 7 A3
• Gene Summary: Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function]