9030624J02Rik (NM_027815) Mouse Untagged Clone

CAT#: MC222999

9030624J02Rik (untagged) - Mouse RIKEN cDNA 9030624J02 gene (9030624J02Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 9030624J02Rik
• Synonyms: 2610029J22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222999 representing NM_027815
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTGGGGCGGGGCCGAGCTTCCCTGGATGTGAGAAAAGGGGCTGGACGCGGAAAACAGGCGATC
  ATGTGACTGGGAAGATGGCTGTCTTTCCCTGGCATTCCCGGAACAGGAACTACAAGGCGGAGCTGGCGTC
  TTGCAGGTTGGAGACAGTGCCTCTGGAGTGCGGCGACTACCACCCGCTGAAGCCCATCACTGTCACGGAG
  TCAAAGACAAAGAAAGTGAGCCGGAAAGGAAGCACGTCTTCTACCTCCTCCTCCTCTTCCAGCTCCGTGA
  TCGATCCTCTGAGCAGCGTCCTCGATGGCACCGACCCGCTCTCCATGTTTGCAGCCACCTCGGACCCGGC
  AGCCACAGGAACAGTCACAGACAGCTCCAGAAAGAAACGTGATAAGGATGAGAACTCCTTCGTGGGACCT
  GATTTCGAGCCCTGGGCCAACAAACGTGTGGAAATCCTTGCCCGGTACACAACTACAGAGAAGCTGTCTA
  TTAATCTGTTTATGGGATCAGAAAAAGGCAGAGGTGGGGCTGCGGCTTCTGCCATGTCAGAGAAGGTTCG
  GACGCGGCTGGAAGAGCTGGATGACTTTGAGGAGGGATCCCAGAAGGAGCTGCTGAACCTGACCCAGCAG
  GATTATGTGAACCGCATAGAGGAGCTCAACCAGTCTCTGAAGGATGCCTGGGCCTCGGACCAGAAGGTGA
  AGGCTCTGAAGATCGTCATCCAGTGTTCAAAGCTGCTGTCAGACACGAGTGTTATTCAGTTCTACCCAAG
  CAAGTTTGTCCTCATCACCGACATCCTTGATACGTTTGGAAAGCTGGTCTATGAGCGCATCTCCTCGATG
  TGTGTGGACAGCCGCAGTGCCTTGCCAGATCATTTCTCCCCAGAGAACGTGAACGACACAGCCAAGGAGA
  CATGCCTGAATTGGTTTTTCAAGATCGCTTCCATCCGGGAACTCATTCCAAGATTTTATGTGGAAGCATC
  CATCCTGAAGTGTAACAAGTTCCTCTCCAAAACGGGGATTTCAGAGTGCCTGCCCCGACTAACCTGCATG
  ATCAGAGGGATCGGAGACCCACTCGTGTCCGTGTACGCCCGGGCCTACCTGTGTCGGGTGGGGATCGAAG
  TGGCCCCGCACCTCAAGGAGAGCCTCAATAAGAACTTCTTTGACTTCCTGCTGACATTTAAACAGATTCA
  TGGGGACACTGTCCAGAACCAGCTGGTGGCCCAGGGTGTGGAGCTGCTGTCCTATCTCCCTCTGTACTCG
  CCTGCCATGGGCTGGATCTTCCAGTGTGTCTCCTACCACGCTCCAGAGGCTTTGCTGACGGAGATGATGG
  AAAGATGTAAGAAACTAGGGAACAATGCTTTGCTGTTGAACTCCGTGATGTCAGCTTTCAGGGCTGAGTT
  CGTTGCCACCAGGTCTATGGACTTCATCGGCATGATCAAAGAGTGTGACGAGTCCGGCTTCCCCAAGCAT
  CTCCTTTTTCGTTCATTGGGGTTAAACTTGGCTTTGGCTGATCCTCCTGAGAACGATCGACTTCAGATTC
  TCAATGAAGCTTGGAAAGTCATCACTAAACTGAAGAGTCCTCAGGACTACATTAATTGTGCCGAGGTTTG
  GGTAGAGTACACCTGCAGGCATTTCACGAAACGAGAAGTTAACACTGTTCTGGCTGACGTCATCAAGCAC
  ATGACTCCGGATCGAGCATTTGAGGACTCTTACCCCCAGCTCCAGTCAATCATTCAGAAAGTCATCGCCC
  ACTTCCACGATTTCTCAGTTCTTTTCTCAGTGGAAAAGTTTCTACCATTTCTGGACATGTTCCAGAAAGA
  GAGTGTGCGGGTGGAGGTTTGCAAGTGCATCATGGAAGCCTTTATCAAGCACCAGCAGGAGCCAACCAAG
  GACCCTGTCATCCTCAATGCCCTTTTGCACATTTGCAAGACAATGCACGACTCCGTGAACGCTCTCACTC
  TTGAGGATGAGAAAAGGACGCTGGCCCACTTGATCAATGGATTTATAAAAATGGTGTCCTTTGGCCGTGA
  TTTTGAACAGCAGCTCAGCTTTTACGTCGAGTCCAGGTCTATGTTTTGCAACCTGGAACCTGTTCTTGTG
  CAGTTGATCCATAGTGTGAACCGGTTGGCAATGGAAACAAGGAAAGTCATGAAGGGAAACCACTCCAGAA
  AGACAGCTGCGTTTGTCCGGGCCTGTGTTGCCTACTGCTTCATCACCATCCCGTCCCTGGTAGGGATCTT
  CACCCGCCTCAACCTCTACCTGCATTCCGGTCAGGTTGCCTTGGCCAACCAGTGCCTCTCCCAAGCTGAT
  GCTTTCTTCAAAGCCGCCATCGGCCTCGTTCCCGAAGTCCCAAAGACGATAAGCATCGATGGGAAGCTGC
  GGCCGTCGGAGCCCTTCCTACTGGAATTCCTGTGCAATTTCTTCTCTACCTTATTGATTGTGCCGGATCA
  TCCAGAGCACGGAGTCCTATTTCTGGTTCGAGAACTTCTCAACGTGATCCAGGACTACACCTGGGAGGAC
  AGTAGTGATGACAAAATTCGAATCTACACCTCTGTCCTGCACCTGCTGTCTGCCATGAGCCAGGACACCT
  ATCTTTACCACATAGACAAAGTTGACTCCAATGACAGCTTGTACGGGGGAGACTCCAAGTTCCTGGCTGA
  GAACAGCAAGCTGTGTGAGGCTGTGATGGCTCAGATCCTGGAGCACCTGAAAACCCTGGCCAAAGACGAG
  GCTCTGAAGCGACAGAGCCTGCTGGGCCTCTCCTTCTTCAATAGCATCCTAGCCCACGGGGACCTTCGTA
  ACAACAAGCTGAACCAACTCTCTGTCAACCTATGGCACCTGGCACAGAGACACGGCTGTGCAGACACAAG
  GACTATGGTGAAAACTCTGGATTACATCAAGAAGCGAAGCAAACAACCTGACATGAATCATCTGTCAGAG
  CTGGCCCTGAGGCTCCCTCTGCAAACCAGGACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027815
• Insert Size: 2976 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027815.4, NP_082091.3
• RefSeq Size: 3578 bp
• RefSeq ORF: 2976 bp
• Locus ID: 71517
• UniProt ID: Q8BWQ6
• Cytogenetics: 7 F2
• Gene Summary: Acts as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. In the endosomes, drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association with the CCC complex and cooperation with the WASH complex on early endosomes. Seems not to be required for CCC complex stability.[UniProtKB/Swiss-Prot Function]